公开(公告)号：US20240247247A1

公开(公告)日：2024-07-25

申请号：US18470879

申请日：2023-09-20

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem ISMAGILOV ,  Erik JUE ,  Daan WITTERS

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6806 ,  G01N21/64

CPC classification number: C12N15/1003 ,  B01L3/502753 ,  B01L3/508 ,  B01L3/50851 ,  B01L7/52 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N21/6428 ,  G01N21/6456 ,  B01L2200/0631 ,  B01L2200/0689 ,  B01L2300/04 ,  B01L2300/0672 ,  B01L2300/069 ,  B01L2300/18 ,  B01L2400/0605

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

公开(公告)号：US20220098573A1

公开(公告)日：2022-03-31

申请号：US17473832

申请日：2021-09-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem ISMAGILOV ,  Erik JUE ,  Daan WITTERS

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6806 ,  B01L7/00 ,  G01N21/64

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

公开(公告)号：US20210189379A1

公开(公告)日：2021-06-24

申请号：US17131449

申请日：2020-12-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Erik JUE ,  Daan WITTERS

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.

公开(公告)号：US20170225161A1

公开(公告)日：2017-08-10

申请号：US15504283

申请日：2015-08-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Stefano BEGOLO ,  Dmitriy V. ZHUKOV ,  Daan WITTERS ,  Erik B. JUE ,  Rustem F. ISMAGILOV

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/14 ,  B01L2400/0475 ,  B01L2400/0478 ,  B01L2400/0487 ,  C12Q1/686

Abstract: Provided herein are devices and methods for generating positive and negative pressures. The devices and methods are suited for the generation of pressures; in particular, the pressures generated can be useful for controlling the flow of fluids, such as in fluidic device.