公开(公告)号：US20160310637A1

公开(公告)日：2016-10-27

申请号：US15004796

申请日：2016-01-22

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA

发明人： Yu-Chong Tai ,  Bo Lu ,  Mark Humayun

IPC分类号： A61L27/50 ,  A61L27/38 ,  A61L27/02 ,  A61F2/02 ,  A61L27/34

CPC分类号： A61L27/50 ,  A61F2/02 ,  A61F2/04 ,  A61F2240/001 ,  A61L27/025 ,  A61L27/18 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  A61L2420/02 ,  A61L2430/16 ,  C12N5/0068 ,  C12N2533/30 ,  G03F7/2002 ,  C08L65/04

摘要： Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The application further provides an implantable cage-like apparatus for culturing cells comprising the parylene membrane.

摘要翻译： 公开了具有较厚区域散布的具有光滑前侧和超薄区域（例如，0.01μm至5μm厚）的薄聚对二甲苯C膜。 与光滑的前侧相比，膜的背面可能是粗糙的。 膜可以在体外用于在实验室或体内生长细胞的单层，作为外科手术植入的生长层，例如替代眼睛中的Bruch膜。 本申请还提供了一种用于培养包含对二甲苯膜的细胞的可植入笼状装置。

公开(公告)号：US09182387B2

公开(公告)日：2015-11-10

申请号：US14020313

申请日：2013-09-06

申请人： California Institute of Technology ,  University of Southern California

发明人： Amir Goldkorn ,  Yu-Chong Tai ,  Tong Xu ,  Bo Lu

IPC分类号： G01N33/50 ,  C12N5/09 ,  C12Q1/68

CPC分类号： G01N33/57496 ,  C12N5/0693 ,  C12N2503/00 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Y207/07049 ,  G01N1/30 ,  G01N1/4077 ,  G01N33/5094 ,  G01N2001/4088 ,  G01N2333/9128 ,  Y10T436/25375

摘要： The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.

摘要翻译： 本发明提供一种用于诊断癌症的方法，预测患者样品中的疾病结果或对治疗的反应。 该方法包括使用聚对二甲苯微型过滤器装置从样品中分离循环肿瘤细胞（CTC），例如可行的CTC，所述聚对二甲苯微型过滤器包括具有具有预定形状的孔阵列的具有聚对二甲苯衬底或由聚对二甲苯衬底组成的膜过滤器， 尺寸; 并检测和定量血液循环肿瘤细胞中的端粒酶活性。 本发明还提供了使用在各种应用中实时捕获的细胞的方法。

公开(公告)号：US20140038271A1

公开(公告)日：2014-02-06

申请号：US14020313

申请日：2013-09-06

申请人： University of Southern California ,  California Institute of Technology

发明人： Amir Goldkorn ,  Yu-Chong Tai ,  Tong Xu ,  Bo Lu

IPC分类号： G01N33/50

CPC分类号： G01N33/57496 ,  C12N5/0693 ,  C12N2503/00 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Y207/07049 ,  G01N1/30 ,  G01N1/4077 ,  G01N33/5094 ,  G01N2001/4088 ,  G01N2333/9128 ,  Y10T436/25375

摘要： The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.

摘要翻译： 本发明提供一种用于诊断癌症的方法，预测患者样品中的疾病结果或对治疗的反应。 该方法包括使用聚对二甲苯微型过滤器装置从样品中分离循环肿瘤细胞（CTC），例如可行的CTC，所述聚对二甲苯微型过滤器包括具有具有预定形状的孔阵列的具有聚对二甲苯衬底或由聚对二甲苯衬底组成的膜过滤器， 尺寸; 并检测和定量血液循环肿瘤细胞中的端粒酶活性。 本发明还提供了使用在各种应用中实时捕获的细胞的方法。

公开(公告)号：US11318225B2

公开(公告)日：2022-05-03

申请号：US15184934

申请日：2016-06-16

申请人： California Institute of Technology ,  University of Southern California

发明人： Yu-Chong Tai ,  Bo Lu ,  Mark Humayun

IPC分类号： C12N5/00 ,  A61L27/16 ,  B01D71/44 ,  A61L27/50 ,  A61L27/56

摘要： Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.

公开(公告)号：US09642940B2

公开(公告)日：2017-05-09

申请号：US15004796

申请日：2016-01-22

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA

发明人： Yu-Chong Tai ,  Bo Lu ,  Mark Humayun

IPC分类号： G03F7/20 ,  A61L27/18 ,  A61L27/50 ,  A61F2/04 ,  C12N5/00 ,  A61L27/38 ,  A61F2/02 ,  A61L27/02 ,  A61L27/34

CPC分类号： A61L27/50 ,  A61F2/02 ,  A61F2/04 ,  A61F2240/001 ,  A61L27/025 ,  A61L27/18 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  A61L2420/02 ,  A61L2430/16 ,  C12N5/0068 ,  C12N2533/30 ,  G03F7/2002 ,  C08L65/04

摘要： Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The application further provides an implantable cage-like apparatus for culturing cells comprising the parylene membrane.

公开(公告)号：US20160252514A1

公开(公告)日：2016-09-01

申请号：US14877711

申请日：2015-10-07

申请人： California Institute of Technology ,  University of Southern California

发明人： Amir Goldkorn ,  Yu-Chong Tai ,  Tong Xu ,  Bo Lu

IPC分类号： G01N33/574 ,  G01N1/30 ,  G01N1/40 ,  C12Q1/68

CPC分类号： G01N33/57496 ,  C12N5/0693 ,  C12N2503/00 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Y207/07049 ,  G01N1/30 ,  G01N1/4077 ,  G01N33/5094 ,  G01N2001/4088 ,  G01N2333/9128 ,  Y10T436/25375

摘要： The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.

摘要翻译： 本发明提供一种用于诊断癌症的方法，预测患者样品中的疾病结果或对治疗的反应。 该方法包括使用聚对二甲苯微型过滤器装置从样品中分离循环肿瘤细胞（CTC），例如可行的CTC，所述聚对二甲苯微型过滤器包括具有具有预定形状的孔阵列的具有聚对二甲苯衬底或由聚对二甲苯衬底组成的膜过滤器， 尺寸; 并检测和定量血液循环肿瘤细胞中的端粒酶活性。 本发明还提供了使用在各种应用中实时捕获的细胞的方法。

公开(公告)号：US20150147810A1

公开(公告)日：2015-05-28

申请号：US14498918

申请日：2014-09-26

申请人： California Institute of Technology ,  University of Southern California

发明人： Yu-Chong Tai ,  Bo Lu ,  Mark Humayun

IPC分类号： C12N5/00

CPC分类号： A61L27/16 ,  A61L27/50 ,  A61L27/56 ,  A61L2430/16 ,  B01D71/44 ,  B01D2325/06 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2535/10 ,  C12N2539/00

摘要： Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.

摘要翻译： 公开了具有较厚区域散布的具有光滑前侧和超薄区域（例如，0.01μm至5μm厚）的薄聚对二甲苯C膜。 与光滑的前侧相比，膜的背面可能是粗糙的。 膜可以在体外用于在实验室或体内生长细胞的单层，作为外科手术植入的生长层，例如替代眼睛中的Bruch膜。 聚对二甲苯的薄区域是半渗透性的，以允许血清中的蛋白质通过，并且厚区域给予外科医生处理的机械支持。 光滑的前侧允许单层细胞生长，粗糙的背面有助于防止细胞附着在那里。

公开(公告)号：US10261088B2

公开(公告)日：2019-04-16

申请号：US14877711

申请日：2015-10-07

申请人： California Institute of Technology ,  University of Southern California

发明人： Amir Goldkorn ,  Yu-Chong Tai ,  Tong Xu ,  Bo Lu

IPC分类号： G01N33/574 ,  C12N5/09 ,  C12Q1/6806 ,  C12Q1/6886 ,  G01N33/50 ,  G01N1/30 ,  G01N1/40

摘要： The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.

公开(公告)号：US20160361463A1

公开(公告)日：2016-12-15

申请号：US15184934

申请日：2016-06-16

申请人： California Institute of Technology ,  University of Southern California

发明人： Yu-Chong Tai ,  Bo Lu ,  Mark Humayun

IPC分类号： A61L27/16 ,  A61L27/56 ,  C12N5/00 ,  A61L27/50

CPC分类号： A61L27/16 ,  A61L27/50 ,  A61L27/56 ,  A61L2430/16 ,  B01D71/44 ,  B01D2325/06 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2535/10 ,  C12N2539/00

摘要： Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.

公开(公告)号：US09248013B2

公开(公告)日：2016-02-02

申请号：US13740069

申请日：2013-01-11

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA

发明人： Yu-Chong Tai ,  Bo Lu ,  Mark Humayun

IPC分类号： A61F2/04 ,  C12N5/00 ,  A61L27/18 ,  A61L27/38

CPC分类号： A61L27/50 ,  A61F2/02 ,  A61F2/04 ,  A61F2240/001 ,  A61L27/025 ,  A61L27/18 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  A61L2420/02 ,  A61L2430/16 ,  C12N5/0068 ,  C12N2533/30 ,  G03F7/2002 ,  C08L65/04

摘要： Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The application further provides an implantable cage-like apparatus for culturing cells comprising the parylene membrane.

摘要翻译： 公开了具有较厚区域散布的具有光滑前侧和超薄区域（例如，0.01μm至5μm厚）的薄聚对二甲苯C膜。 与光滑的前侧相比，膜的背面可能是粗糙的。 膜可以在体外用于在实验室或体内生长细胞的单层，作为外科手术植入的生长层，例如替代眼睛中的Bruch膜。 本申请还提供了一种用于培养包含对二甲苯膜的细胞的可植入笼状装置。