公开(公告)号：US09494557B2

公开(公告)日：2016-11-15

申请号：US14001963

申请日：2012-04-05

申请人： Cagri A. Savran ,  Chung-Li Chang ,  Philip S. Low

发明人： Cagri A. Savran ,  Chung-Li Chang ,  Philip S. Low

IPC分类号： G01N33/553 ,  G01N27/74 ,  G01N33/543 ,  B01L3/00 ,  G01N33/574 ,  G01N1/40

CPC分类号： B01L3/502761 ,  B01L3/50273 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L2400/043 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/574 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2001/4016

摘要： A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target cells bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target cells from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target cells bound to magnetic beads on the surface of the plate. The microfluidic detection system includes a pump flowing the fluid through the detector component at high flow rates of milliliters per minute for high throughput detection of target cells.

摘要翻译： 用于微米尺寸实体（例如生物细胞）的微流体检测系统包括结合有多个开口的板的检测器部件，该板分离两个室，一个与包含与磁珠结合的靶细胞的流体源连通。 这些开口的尺寸设置成总是允许磁珠通过其进入下部的一个室，并且进一步的尺寸设计成总是防止靶细胞从上部的一个室中通过。 检测器部件还包括磁铁，该磁体定位成将未结合的磁珠拉过开口并捕获与板表面上的磁珠结合的靶细胞。 微流体检测系统包括泵以高分子量/分钟的速度流过检测器部件，用于目标细胞的高通量检测。

公开(公告)号：US20140024049A1

公开(公告)日：2014-01-23

申请号：US14001963

申请日：2012-04-05

申请人： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang

发明人： Cagri A. Savran ,  Philip S. Low ,  Chun-Li Chang

IPC分类号： G01N27/74

CPC分类号： B01L3/502761 ,  B01L3/50273 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L2400/043 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/574 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2001/4016

摘要： A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target cells bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target cells from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target cells bound to magnetic beads on the surface of the plate. The microfluidic detection system includes a pump flowing the fluid through the detector component at high flow rates of milliliters per minute for high throughput detection of target cells.

摘要翻译： 用于微米尺寸实体（例如生物细胞）的微流体检测系统包括结合有多个开口的板的检测器部件，该板分离两个室，一个与包含与磁珠结合的靶细胞的流体源连通。 这些开口的尺寸设置成总是允许磁珠通过其进入下部的一个室，并且进一步的尺寸设计成总是防止靶细胞从上部的一个室中通过。 检测器部件还包括磁铁，该磁体定位成将未结合的磁珠拉过开口并捕获与板表面上的磁珠结合的靶细胞。 微流体检测系统包括泵以高分子量/分钟的速度流过检测器部件，用于目标细胞的高通量检测。

公开(公告)号：US20120241596A1

公开(公告)日：2012-09-27

申请号：US13430316

申请日：2012-03-26

申请人： Cagri A. Savran ,  Khalid M. Arif

发明人： Cagri A. Savran ,  Khalid M. Arif

IPC分类号： H01L27/146 ,  H01L27/148 ,  H01L31/14

CPC分类号： G01N21/55 ,  G01N21/4788 ,  G01N21/553 ,  G01N21/554 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/479 ,  G01N2021/558 ,  G01N2201/0221 ,  G01N2201/0693 ,  G02B21/0008 ,  G02B21/18 ,  G02B21/36

摘要： A system for and method of performing multi-technique imaging are disclosed. Such multi-technique imaging system includes a surface for supporting a specimen and at least two illumination sources for producing light radiation. The system also includes a plurality of reflective and refractive devices arranged to direct at least part of the light radiation from each of the at least two illumination sources to the surface such that the at least part of the light radiation from each of the at least two illumination sources illuminates substantially the same area on the surface. The system also includes a sensor configured to receive light radiation from the at least two illumination sources reflected by the specimen and/or that pass by the specimen. The system also includes a power source configured to power the at least two illumination sources and the sensor.

摘要翻译： 公开了一种执行多技术成像的系统和方法。 这种多技术成像系统包括用于支撑样本的表面和用于产生光辐射的至少两个照明源。 该系统还包括多个反射和折射装置，其布置成将来自至少两个照射源中的每一个的光辐射的至少一部分引导到表面，使得来自至少两个照射源中的每一个的光辐射的至少一部分 照明源基本上照射在表面上相同的区域。 该系统还包括被配置为接收来自由样本反射的至少两个照明源的光辐射和/或由样本通过的传感器。 该系统还包括被配置为对至少两个照明源和传感器供电的电源。

公开(公告)号：US08933384B2

公开(公告)日：2015-01-13

申请号：US13430316

申请日：2012-03-26

申请人： Cagri A. Savran ,  Khalid M. Arif

发明人： Cagri A. Savran ,  Khalid M. Arif

IPC分类号： G02B27/00 ,  G02B7/00 ,  H01L27/00 ,  G01N21/55 ,  G02B21/00 ,  G02B21/18 ,  G02B21/36 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65

CPC分类号： G01N21/55 ,  G01N21/4788 ,  G01N21/553 ,  G01N21/554 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/479 ,  G01N2021/558 ,  G01N2201/0221 ,  G01N2201/0693 ,  G02B21/0008 ,  G02B21/18 ,  G02B21/36

摘要： A system for and method of performing multi-technique imaging. Such multi-technique imaging system includes a surface for supporting a specimen and at least two illumination sources for producing light radiation. The system also includes a plurality of reflective and refractive devices arranged to direct at least part of the light radiation from each of the at least two illumination sources to the surface such that at least part of the light radiation from each of the at least two illumination sources illuminates substantially the same area on the surface. The system also includes a sensor configured to receive light radiation from the at least two illumination sources reflected by the specimen and/or that pass by the specimen. The system also includes a power source configured to power the at least two illumination sources and the sensor.

摘要翻译： 一种执行多技术成像的系统和方法。 这种多技术成像系统包括用于支撑样本的表面和用于产生光辐射的至少两个照明源。 该系统还包括多个反射和折射装置，其布置成将来自至少两个照明源中的每一个的光辐射的至少一部分引导到表面，使得来自至少两个照射中的每一个的至少部分光辐射 光源基本上照射在表面上相同的区域。 该系统还包括被配置为接收来自由样本反射的至少两个照明源的光辐射和/或由样本通过的传感器。 该系统还包括被配置为对至少两个照明源和传感器供电的电源。