公开(公告)号：US20030061687A1

公开(公告)日：2003-04-03

申请号：US10117978

申请日：2002-04-05

Applicant: California Institute of Technology, A California Corporation

Inventor： Carl L. Hansen ,  Stephen R. Quake ,  James M. Berger

IPC: B01D009/02

CPC classification number: C30B7/08 ,  B01J2219/00274 ,  B01L3/06 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/049 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/0688 ,  C12Q1/6874 ,  C30B7/14 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0026 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T117/1004 ,  Y10T117/1008 ,  Y10T137/0318 ,  Y10T137/0396 ,  C12Q2535/125

Abstract: High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device. The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers, thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.

Abstract translation: 目标材料的结晶化的高通量筛选是通过将目标材料的溶液同时引入微细加工的流体装置的多个室来实现的。 然后对微制造的流体装置进行操作以改变室中的溶液状态，从而同时提供大量的结晶环境。 改变的溶液条件的控制可以由各种技术产生，包括但不限于通过体积排阻将结晶剂计量到室中的体积，通过捕获通过微结构结构的尺寸确定的结晶剂的体积， 将样品和结晶剂通道注入由相交的正交流动通道限定的连接阵列。

公开(公告)号：US20030096310A1

公开(公告)日：2003-05-22

申请号：US10265473

申请日：2002-10-04

Applicant: California Institute of Technology

Inventor： Carl L. Hansen ,  Stephen R. Quake ,  James M. Berger

IPC: G01N033/536

CPC classification number: C30B7/14 ,  B01F5/00 ,  B01F13/0093 ,  B01J2219/00355 ,  B01J2219/00378 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00439 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502769 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0688 ,  B81B1/00 ,  C30B29/02 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0017 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0086 ,  Y10T117/1004 ,  Y10T117/1008 ,  Y10T117/1012 ,  Y10T137/0318 ,  Y10T137/0324 ,  Y10T137/2224 ,  Y10T436/25

Abstract: A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.

Abstract translation: 将静态流体和第二流体沿微流体界面放置接触，并允许通过扩散混合而不对流流过该界面。 根据本发明的一个实施例，流体是静态的，并且最初位于微流体通道中的封闭阀结构的任一侧上，该微流体通道的宽度被紧紧地约束在至少一个维度上。 然后打开阀门，并且微流体通道两侧的防滑层抑制沿着所得界面的两种流体之间的对流混合。 根据本发明的实施方案的用于微流体自由界面的应用包括但不限于蛋白质结晶研究，蛋白质溶解度研究，流体系统的性质的确定以及各种生物测定如扩散免疫测定，底物转换测定 和竞争性结合测定。