公开(公告)号：US10434219B2

公开(公告)日：2019-10-08

申请号：US15859304

申请日：2017-12-29

申请人： CellSeed Inc. ,  Kohji Nishida

发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

IPC分类号： A61L27/38 ,  C12N5/079

摘要： A diseased site where an anterior segment tissue is partly or entirely damaged or deficient can be treated using a corneal epithelium forming cell sheet that will adhere well to the anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0° C.-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

公开(公告)号：US09981064B2

公开(公告)日：2018-05-29

申请号：US10546275

申请日：2004-02-20

申请人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

IPC分类号： C12N5/00 ,  A61L27/38 ,  A61L27/50 ,  C12N5/079

CPC分类号： A61L27/3808 ,  A61L27/3839 ,  A61L27/3895 ,  A61L27/50 ,  A61L2430/16 ,  C12N5/0621 ,  C12N2539/10

摘要： An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80° C., and after the culture,(1) adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated,(2) bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and(3) detaching the sheet together with the carrier.The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.

公开(公告)号：US20070148137A1

公开(公告)日：2007-06-28

申请号：US10546275

申请日：2004-02-20

申请人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

IPC分类号： A61K35/30 ,  C12N5/08

CPC分类号： A61L27/3808 ,  A61L27/3839 ,  A61L27/3895 ,  A61L27/50 ,  A61L2430/16 ,  C12N5/0621 ,  C12N2539/10

摘要： An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80° C., and after the culture, (1) adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated, (2) bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and (3) detaching the sheet together with the carrier. The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.

摘要翻译： 一种用于生产再生角膜内皮细胞片的改进方法，包括以下步骤：将从组织收集的角膜内皮细胞培养在细胞培养支持物上，所述细胞培养支持物的表面被聚合物覆盖，所述聚合物的水合力在温度范围 0-80℃，培养后，（1）将培养液的温度调节至基材表面的聚合物水合的温度，（2）使培养的角膜内皮细胞片与 载体，和（3）与载体一起分离片材。 通过该方法获得的再生角膜内皮细胞片材将很好地粘附到活组织。

公开(公告)号：US20060234377A1

公开(公告)日：2006-10-19

申请号：US10544541

申请日：2004-02-06

申请人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

IPC分类号： C12N5/08

CPC分类号： A61L27/3891 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/16 ,  C12N5/0621 ,  C12N2539/10

摘要： The present invention has as its objective providing acorneal epithelium forming cell sheet that will adhere well to an anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

摘要翻译： 本发明的目的是提供能够很好地粘附于前段组织的上皮细胞上皮细胞片。 为了实现该目的，通过包括以下步骤的方法制备角膜上皮细胞片，该方法包括以下步骤：在细胞培养载体上在特定条件下培养角膜上皮形成细胞，所述细胞培养载体包含其表面覆盖有温度响应性聚合物的底物，所述温度响应性聚合物的水合力 在0-80℃的温度范围内变化，任选地分层培养细胞层，然后（1）将培养溶液的温度调节至高于上临界溶解温度或低于临界溶解温度以下， （2）使培养的角膜上皮形成细胞与载体紧密接触，和（3）在规定的条件下将载片与载体一起分离。

公开(公告)号：US10368980B2

公开(公告)日：2019-08-06

申请号：US15098604

申请日：2016-04-14

申请人： Tokyo Women's Medical University ,  The Jikei University ,  CellSeed Inc.

发明人： Takanori Hama ,  Hiromi Kojima ,  Hiroshi Moriyama ,  Masayuki Yamato ,  Teruo Okano

IPC分类号： A61F2/18 ,  C12N5/0793 ,  A61L27/38 ,  A61K35/12

摘要： The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

公开(公告)号：US09889228B2

公开(公告)日：2018-02-13

申请号：US14228212

申请日：2014-03-27

申请人： CELLSEED INC.

发明人： Masato Kanzaki ,  Masayuki Yamato ,  Tinatsu Kohno ,  Hidekazu Sekine ,  Akihiko Kikuchi ,  Teruo Okano ,  Takamasa Onuki

IPC分类号： A61L27/38 ,  A61L27/34 ,  C12N5/00 ,  C12N5/071

CPC分类号： A61L27/3895 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  C12N5/0068 ,  C12N5/0688 ,  C12N2533/30

摘要： The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.

公开(公告)号：US20160220356A1

公开(公告)日：2016-08-04

申请号：US15098604

申请日：2016-04-14

申请人： Tokyo Women's Medical University ,  The Jikei University ,  CellSeed Inc.

发明人： Takanori Hama ,  Hiromi Kojima ,  Hiroshi Moriyama ,  Masayuki Yamato ,  Teruo Okano

IPC分类号： A61F2/18 ,  A61L27/38

CPC分类号： A61F2/18 ,  A61F2002/183 ,  A61F2310/00365 ,  A61K35/12 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3839 ,  A61L27/3886 ,  A61L2430/14 ,  C12N5/062 ,  C12N2533/30

摘要： The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

摘要翻译： 本发明的目的是获得中耳粘膜替代物，其中植入中耳骨的表面，造粒组织和骨的增生以及成纤维细胞的发育 在中耳被抑制，并且获得在表层中保留纤毛的中耳粘膜样细胞片，包括在涂覆有聚合物的细胞培养基质上培养鼻上皮细胞，其水合力在0至 80℃，其中在聚合物的水合力弱的温度范围内培养细胞，然后将温度改变到水合力强的温度以回收培养的细胞片。