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    CULTURED CELL SHEET, PRODUCTION METHOD, AND TISSUE REPAIR METHOD USING THEREOF
    2.
    发明申请
    CULTURED CELL SHEET, PRODUCTION METHOD, AND TISSUE REPAIR METHOD USING THEREOF 有权
    培养细胞片，生产方法和使用其的组织修复方法

    公开(公告)号：US20140212470A1

    公开(公告)日：2014-07-31

    申请号：US14228212

    申请日：2014-03-27

    Applicant: CELLSEED INC.

    Inventor： Masato KANZAKI ,  Masayuki YAMATO ,  Tinatsu KOHNO ,  Hidekazu SEKINE ,  Akihiko KIKUCHI ,  Teruo OKANO ,  Takamasa ONUKI

    IPC: A61L27/38 ,  C12N5/071

    CPC classification number: A61L27/3895 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  C12N5/0068 ,  C12N5/0688 ,  C12N2533/30

    Abstract: The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.

    Abstract translation: 本发明要解决的问题是提供具有优异组织粘附性和优异柔韧性的培养细胞片。 可以通过在表面活性蛋白或交联抑制剂的存在下培养细胞培养的细胞片来解决问题，所述细胞片在涂覆有具有上或下的温度响应性聚合物的细胞培养载体的表面上 包括以下步骤：将培养基的温度调节到低于临界溶解温度以下或高于临界溶解温度以上的温度; 并分离培养的细胞片。

    METHOD OF CONSTRUCTING ANIMAL HAVING CANCER CELLS TRANSPLANTED THEREINTO
    3.
    发明申请
    METHOD OF CONSTRUCTING ANIMAL HAVING CANCER CELLS TRANSPLANTED THEREINTO 审中-公开
    构建动物移植癌细胞的方法

    公开(公告)号：US20140056814A1

    公开(公告)日：2014-02-27

    申请号：US13945880

    申请日：2013-07-18

    Applicant: CELLSEED INC.

    Inventor： Teruo OKANO ,  Akihiko KIKUCHI ,  Masayuki YAMATO ,  Akira MASUDA

    IPC: A01K67/027 ,  C12N5/09

    CPC classification number: A01K67/0271 ,  A01K2227/105 ,  A01K2267/0331 ,  A61K49/0008 ,  C12N5/0693

    Abstract: A cell culture support is first prepared that is coated on a surface with a polymer the hydration force of which changes in a temperature range of 0-80° C.; cancer cells are then cultivated on the support in a temperature region where the polymer has weak hydration force; thereafter, the culture solution is adjusted to a temperature at which the polymer has a stronger hydration force, whereby the cultured cancer cells are detached; the detached cancer cells are then transplanted to a specified site of an animal on which transplantation is to be performed; this method is an efficient way of cancer cells transplantation.

    Abstract translation: 首先制备细胞培养载体，其在表面上涂覆聚合物，其水合力在0-80℃的温度范围内变化; 然后将癌细胞在聚合物具有弱水合力的温度区域中在载体上培养; 此后，将培养液调整为聚合物具有更强的水合力的温度，由此分离培养的癌细胞。 然后将分离的癌细胞移植到要进行移植的动物的特定部位; 这种方法是癌细胞移植的有效途径。

    METHOD OF TREATMENT USING CORNEAL EPITHELIUM FORMING CELL SHEETS
    5.
    发明申请
    METHOD OF TREATMENT USING CORNEAL EPITHELIUM FORMING CELL SHEETS 审中-公开

    公开(公告)号：US20180126040A1

    公开(公告)日：2018-05-10

    申请号：US15859304

    申请日：2017-12-29

    Applicant: Kohji NISHIDA ,  CellSeed Inc.

    Inventor： Teruo OKANO ,  Kohji NISHIDA ,  Masayuki YAMATO

    IPC: A61L27/38 ,  C12N5/079

    CPC classification number: A61L27/3891 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/16 ,  C12N5/0621 ,  C12N2539/10

    Abstract: A diseased site where an anterior segment tissue is partly or entirely damaged or deficient can be treated using a corneal epithelium forming cell sheet that will adhere well to the anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0° C.-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

    THREE-DIMENSIONAL TISSUE STRUCTURE
    6.
    发明申请
    THREE-DIMENSIONAL TISSUE STRUCTURE 审中-公开
    三维组织结构

    公开(公告)号：US20150110756A1

    公开(公告)日：2015-04-23

    申请号：US14524212

    申请日：2014-10-27

    Applicant: CELLSEED, INC.

    Inventor： Hikaru MATSUDA ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa

    IPC: C12N5/077 ,  C12N5/00 ,  A61K35/34

    CPC classification number: C12N5/0658 ,  A61K35/12 ,  A61K35/34 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/20 ,  C12N5/0068 ,  C12N5/0657 ,  C12N2506/02 ,  C12N2506/1392 ,  C12N2513/00 ,  C12N2523/00 ,  C12N2533/40 ,  C12N2539/10

    Abstract: The present invention provides a prosthetic tissue or sheet capable of withstanding implantation operations, which can be used in actual operation and can be produced by culture. The present invention also provides a novel therapy which can substitute for cell therapy. Particularly, the present invention provides a method for producing a prosthetic tissue comprising a cell derived from a part other than myocardium and capable of withstanding implantation operation. The above-described objects of the present invention were partially achieved by finding that by culturing cells under specific culture conditions, the cells are unexpectedly organized into a tissue, and the resultant prosthetic tissue is capable of being detached from culture dishes. The present invention also provides a three-dimensional structure applicable to heart, comprising a cell derived from a part other than the myocardium of an adult.

    Abstract translation: 本发明提供能够承受植入操作的假体组织或片材，其可以在实际操作中使用并且可以通过培养产生。 本发明还提供了可代替细胞治疗的新型治疗方法。 特别地，本发明提供了一种用于制造假体组织的方法，所述假体组织包含源于除了心肌之外的部分的细胞并且能够承受植入操作。 通过发现通过在特定培养条件下培养细胞，细胞意外地组织成组织，并且所得到的假体组织能够从培养皿中分离，部分实现了本发明的上述目的。 本发明还提供了适用于心脏的三维结构，其包含源自成人心肌之外的部分的细胞。

    Method of treatment using corneal epithelium forming cell sheets
    9.
    发明授权
    Method of treatment using corneal epithelium forming cell sheets 有权

    公开(公告)号：US10434219B2

    公开(公告)日：2019-10-08

    申请号：US15859304

    申请日：2017-12-29

    Applicant: CellSeed Inc. ,  Kohji Nishida

    Inventor： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

    IPC: A61L27/38 ,  C12N5/079

    Abstract: A diseased site where an anterior segment tissue is partly or entirely damaged or deficient can be treated using a corneal epithelium forming cell sheet that will adhere well to the anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0° C.-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

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