公开(公告)号：US20080186801A1

公开(公告)日：2008-08-07

申请号：US12014813

申请日：2008-01-16

申请人： Cheng-Hsien Liu ,  Long Hsu ,  Chih-Ming Cheng ,  Sheng-Hung Chiu ,  Wai William Wang ,  Chung-Cheng Chou

发明人： Cheng-Hsien Liu ,  Long Hsu ,  Chih-Ming Cheng ,  Sheng-Hung Chiu ,  Wai William Wang ,  Chung-Cheng Chou

IPC分类号： B01F13/00 ,  B01F3/08 ,  B01F15/02 ,  F04B17/00

CPC分类号： B01F13/0059 ,  B01F5/10 ,  B01F11/0071 ,  F04B17/00 ,  F04B19/006 ,  F04B19/24

摘要： A bubble micro-pump includes a first component, a second component and a bubble-generating unit. The first component includes a flow path having a first area and a second area. The second component above the first component has a first roughness surface and a second roughness surface. The first roughness surface opposite the first area has a first roughness factor, and the second roughness surface opposite the second area has a second roughness factor smaller than the first roughness factor. The bubble-generating unit on the first component generates bubble in the first area and the second area when a fluid fills the vacancy between the first component and the second component. Due to the difference in roughness factor, the backfilling velocity of the fluid in the first area is faster than that in the second area when the bubble starts to vanish, such that the fluid is driven to flow.

摘要翻译： 气泡微型泵包括第一部件，第二部件和气泡发生单元。 第一部件包括具有第一区域和第二区域的流动路径。 第一部件上方的第二部件具有第一粗糙度表面和第二粗糙度表面。 与第一区域相对的第一粗糙表面具有第一粗糙度因子，并且与第二区域相对的第二粗糙度表面具有小于第一粗糙度因子的第二粗糙度因子。 当第一部件和第二部件之间的空隙充满时，第一部件上的气泡发生单元在第一区域和第二区域中产生气泡。 由于粗糙度因子的差异，当气泡开始消失时，第一区域中的流体的回填速度比第二区域的回填速度快，使得流体被驱动流动。

公开(公告)号：US20080174870A1

公开(公告)日：2008-07-24

申请号：US12019316

申请日：2008-01-24

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： G02B27/42

CPC分类号： G02B21/32 ,  G02B5/1842 ,  G02B21/0088

摘要： An apparatus of generating optical tweezers with momentum is provided for providing optical tweezers with a first momentum on a test piece. The apparatus comprises a laser source, a diffractive optical element (DOE) and a lens. The laser source is for outputting a laser beam. The DOE has a first phase-delay picture, and the laser beam forms a diffraction pattern after passing the first phase-delay picture. The lens is for receiving and focusing the diffraction pattern on the test piece to form the first optical tweezers with the first momentum. The lens has an optical axis intersecting the DOE at an optical intersection point, a geometric center of the phase-delay picture has a displacement vector relative to the optical intersection point and a direction of the first momentum is related to a direction of the displacement vector.

摘要翻译： 提供产生具有动量的光学镊子的装置，用于在测试片上提供具有第一动量的光学镊子。 该装置包括激光源，衍射光学元件（DOE）和透镜。 激光源用于输出激光束。 DOE具有第一相位延迟图像，并且激光束在通过第一相位延迟图像之后形成衍射图案。 透镜用于接收和聚焦在测试片上的衍射图案以形成具有第一动量的第一光学镊子。 透镜在光学交点处具有与DOE相交的光轴，相位延迟图像的几何中心具有相对于光学交叉点的位移矢量，并且第一动量的方向与位移矢量的方向相关 。

公开(公告)号：US20080108121A1

公开(公告)日：2008-05-08

申请号：US11797418

申请日：2007-05-03

申请人： Cheng-Hsien Liu ,  Hwan-You Chang ,  Long Hsu ,  Chen-Ta Ho ,  Ruei-Zeng Lin ,  Wai William Wang ,  Ta-Yuan Lee ,  Chung-Cheng Chou ,  Chen Peng ,  Fung-Hsu Wu

发明人： Cheng-Hsien Liu ,  Hwan-You Chang ,  Long Hsu ,  Chen-Ta Ho ,  Ruei-Zeng Lin ,  Wai William Wang ,  Ta-Yuan Lee ,  Chung-Cheng Chou ,  Chen Peng ,  Fung-Hsu Wu

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： C12N5/0697 ,  C12N5/067 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2529/00 ,  C12N2535/10

摘要： A method of arranging cells comprises: (a) applying a voltage to two electrodes so as to allow a plurality of cells suspended in a dielectrophoresis-manipulating buffer (DEP-manipulating buffer) to be driven to be arranged into a pattern; (b) replacing the DEP-manipulating buffer with a solution comprising calcium ion and magnesium ion which helps the patterned cells adhere to the substrate; and (c) replacing the solution comprising calcium ion and magnesium ion with a medium so as to allow the patterned cells to grow on the substrate.

摘要翻译： 一种布置电池的方法包括：（a）向两个电极施加电压，以允许驱动悬浮在介电电泳操纵缓冲器（DEP-操纵缓冲器）中的多个电池被布置成图案; （b）用包含钙离子和镁离子的溶液代替DEP操纵缓冲液，其帮助图案化细胞粘附到基底上; 和（c）用培养基替换包含钙离子和镁离子的溶液，以使图案化细胞在基底上生长。

公开(公告)号：US07838819B2

公开(公告)日：2010-11-23

申请号：US12019316

申请日：2008-01-24

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： H01S1/00 ,  H01S3/00 ,  H05H3/02

CPC分类号： G02B21/32 ,  G02B5/1842 ,  G02B21/0088

摘要： An apparatus of generating optical tweezers with momentum is provided for providing optical tweezers with a first momentum on a test piece. The apparatus comprises a laser source, a diffractive optical element (DOE) and a lens. The laser source is for outputting a laser beam. The DOE has a first phase-delay picture, and the laser beam forms a diffraction pattern after passing the first phase-delay picture. The lens is for receiving and focusing the diffraction pattern on the test piece to form the first optical tweezers with the first momentum. The lens has an optical axis intersecting the DOE at an optical intersection point, a geometric center of the phase-delay picture has a displacement vector relative to the optical intersection point and a direction of the first momentum is related to a direction of the displacement vector.

摘要翻译： 提供产生具有动量的光学镊子的装置，用于在测试片上提供具有第一动量的光学镊子。 该装置包括激光源，衍射光学元件（DOE）和透镜。 激光源用于输出激光束。 DOE具有第一相位延迟图像，并且激光束在通过第一相位延迟图像之后形成衍射图案。 透镜用于接收和聚焦在测试片上的衍射图案以形成具有第一动量的第一光学镊子。 透镜在光学交点处具有与DOE相交的光轴，相位延迟图像的几何中心具有相对于光学交叉点的位移矢量，并且第一动量的方向与位移矢量的方向相关 。

公开(公告)号：US20110203354A1

公开(公告)日：2011-08-25

申请号：US13098599

申请日：2011-05-02

申请人： Yuh-Shyong Yang ,  William Wang ,  Long Hsu ,  Cheng-Hsien Liu ,  Chung-Cheng Chou

发明人： Yuh-Shyong Yang ,  William Wang ,  Long Hsu ,  Cheng-Hsien Liu ,  Chung-Cheng Chou

IPC分类号： G01N33/49

CPC分类号： B03C5/005 ,  B01L3/502761 ,  B01L3/502792 ,  B01L9/527 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B03C5/026 ,  B03C2201/26 ,  B03C2201/32 ,  G01N21/05 ,  G01N21/31 ,  G01N33/491 ,  G01N2021/0325 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/062

摘要： A continuous testing method for testing the concentration of a target object in a fluid is provided. The method comprises the following steps. A focused light is provided in the fluid to separate the target object from a non-target object in the fluid by changing the movement direction of the target object and the non-target object. The fluid having separated out the non-target object is enabled to react with a reagent. A signal is provided to pass through the fluid having reacted with the reagent. The signal passing through the fluid is received and an electronic signal is outputted corresponding to the input signal. The concentration of the target object is acquired according to the electronic signal.

摘要翻译： 提供了用于测试流体中目标物体的浓度的连续测试方法。 该方法包括以下步骤。 在流体中设置聚焦光以通过改变目标物体和非目标物体的运动方向将目标物体与流体中的非目标物体分离。 分离出非目标物体的液体能够与试剂反应。 提供信号以通过与试剂反应的流体。 接收通过流体的信号，并根据输入信号输出电子信号。 根据电子信号获取目标物体的浓度。

公开(公告)号：US07829839B2

公开(公告)日：2010-11-09

申请号：US12285958

申请日：2008-10-17

申请人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

发明人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

IPC分类号： H05H3/00 ,  C25B9/16

CPC分类号： B03C5/026 ,  G01N35/10 ,  G01N2035/1046

摘要： An optical tweezers lifting apparatus is provided. The optical tweezers lifting apparatus includes an optical tweezers and a particle-lifting device. The particle-lifting device includes a substrate and a plurality of electrodes that are disposed on the bottom of a flow path in the substrate. When a dielectrophoresis (DEP) solution with a plurality of floating particles is conducted into the flow path and upon those electrodes and a voltage is applied to these electrodes, these particles would be driven by a negative DEP force to move upward to a specific depth in the flow path. Meanwhile, the optical tweezers of the apparatus is selectively focused at the specific depth in the flow path.

摘要翻译： 提供一种光学镊子提升装置。 光学镊子提升装置包括光学镊子和颗粒提升装置。 颗粒提升装置包括基板和设置在基板中的流路底部的多个电极。 当具有多个浮动颗粒的介电电泳（DEP）溶液被引导到流动路径中并且在那些电极上并且向这些电极施加电压时，这些颗粒将被负的DEP力驱动以向上移动到特定的深度 流路。 同时，该设备的光学镊子选择性地聚焦在流路中的特定深度处。

公开(公告)号：US20100108872A1

公开(公告)日：2010-05-06

申请号：US12285958

申请日：2008-10-17

申请人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

发明人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

IPC分类号： H05H3/00 ,  C25B9/16

CPC分类号： B03C5/026 ,  G01N35/10 ,  G01N2035/1046

摘要： An optical tweezers lifting apparatus is provided. The optical tweezers lifting apparatus includes an optical tweezers and a particle-lifting device. The particle-lifting device includes a substrate and a plurality of electrodes that are disposed on the bottom of a flow path in the substrate. When a dielectrophoresis (DEP) solution with a plurality of floating particles is conducted into the flow path and upon those electrodes and a voltage is applied to these electrodes, these particles would be driven by a negative DEP force to move upward to a specific depth in the flow path. Meanwhile, the optical tweezers of the apparatus is selectively focused at the specific depth in the flow path.

公开(公告)号：US20090273831A1

公开(公告)日：2009-11-05

申请号：US12432876

申请日：2009-04-30

申请人： Long Hsu ,  Kuang-Lung Huang ,  William Wang ,  Cheng-Hsien Liu ,  Yuh-Shyong Yang ,  Che-Liang Tsai ,  Chung-Cheng Chou

发明人： Long Hsu ,  Kuang-Lung Huang ,  William Wang ,  Cheng-Hsien Liu ,  Yuh-Shyong Yang ,  Che-Liang Tsai ,  Chung-Cheng Chou

IPC分类号： G02B21/10 ,  H05H3/00

CPC分类号： G02B21/10 ,  G02B21/32

摘要： A light module is provided. The light module applied to a dark field microscope is used for illuminating an object. The light module includes a light beam, a reflection component and a condensing component. The light beam has several lights. The reflection component is used for converting the lights radiating along a beginning direction to a circular beam substantially radiating along the beginning direction. The circular beam passes through the condensing component and is focused on the object. A part of the circular beam passing through the condensing component is scattered by the object.

摘要翻译： 提供灯模块。 应用于暗视野显微镜的光模块用于照亮物体。 光模块包括光束，反射部件和冷凝部件。 光束有几个灯。 反射分量用于将沿开始方向辐射的光转换成沿着开始方向基本辐射的圆形光束。 圆形光束通过冷凝部件并聚焦在物体上。 通过聚光部件的圆形光束的一部分被物体散射。

公开(公告)号：US20090032692A1

公开(公告)日：2009-02-05

申请号：US12219871

申请日：2008-07-30

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  William Wang ,  Chung-Cheng Chou ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  William Wang ,  Chung-Cheng Chou ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： H05H3/00 ,  G02B27/42

CPC分类号： G02B21/32 ,  Y10T436/25375

摘要： An apparatus of generating an optical tweezers with momentum and method thereof and an optical tweezers photo-image for guiding particles are provided. The apparatus generates at least one optical tweezers on an examined object that carries at least one particle. The apparatus includes a laser source, a diffractive optical element and a convergent lens. The laser beam from the laser source passes through the diffractive optical element to produce a diffractive pattern. The laser beam is then received by the convergent lens and then to be focused on a plane of the examined object. The optic axis of the convergent lens is substantially not perpendicular to the plane of the examined object, so that the laser beam is projected onto the plane of the examined object in a skewed manner for providing a lateral momentum to move the particle.

摘要翻译： 提供了一种用于产生具有动量及其方法的光学镊子的装置和用于引导颗粒的光学镊子照片。 该装置在携带至少一个颗粒的被检查物体上产生至少一个光学镊子。 该装置包括激光源，衍射光学元件和会聚透镜。 来自激光源的激光束通过衍射光学元件以产生衍射图案。 然后激光束被会聚透镜接收，然后被聚焦在被检查物体的平面上。 会聚透镜的光轴基本上不垂直于被检查物体的平面，使得激光束以倾斜的方式投射到被检查物体的平面上，以提供横向动量来移动颗粒。

公开(公告)号：US20090052038A1

公开(公告)日：2009-02-26

申请号：US12222250

申请日：2008-08-06

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： G02B27/42

CPC分类号： G02B21/32

摘要： An apparatus and a method for changing optical tweezers are provided. The apparatus includes a diffractive optical element (DOE), a mask unit and an objective lens. The DOE includes a plurality of phase delay patterns. The mask unit includes a plurality of mask patterns that correspond to the phase delay patterns, respectively, wherein at least a portion of the mask patterns are complementary. A laser beam passing through each phase diffractive pattern correspondingly passes through each mask pattern to generate a compound diffractive pattern. The objective lens receives the compound diffractive pattern and focuses it on an examining object to form an optical tweezers.

摘要翻译： 提供了一种用于改变光学镊子的设备和方法。 该装置包括衍射光学元件（DOE），掩模单元和物镜。 DOE包括多个相位延迟模式。 掩模单元包括分别对应于相位延迟图案的多个掩模图案，其中至少一部分掩模图案是互补的。 通过每个相位衍射图案的激光束相应地通过每个掩模图案以产生复合衍射图案。 物镜接收复合衍射图案并将其聚焦在检查对象上以形成光学镊子。