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公开(公告)号：US08258464B2
公开(公告)日：2012-09-04
申请号：US12785945
申请日：2010-05-24
申请人： Chung-Hsuan Chen , Chien-Hsun Chen , Jung-Lee Lin , Ming-Lee Chu
发明人： Chung-Hsuan Chen , Chien-Hsun Chen , Jung-Lee Lin , Ming-Lee Chu
IPC分类号： H01J49/34 , H01J37/244
CPC分类号： H01J49/025 , H01J49/0027
摘要： A mass spectrometer and methods for obtaining the mass spectrum of a single macromolecular or biomolecular ion in a mass spectrometer. The methods include creating single macromolecular or biomolecular primary ions in an ion trap by ionization of a macromolecule or biomolecule; ejecting half of the primary ions for detection with a first charge detector; ejecting half of the primary ions to impact upon a conversion dynode, thereby creating secondary ions for detection with charge amplification detector such as a channeltron or an electromultiplier or an MCP.
摘要翻译： 质谱仪和在质谱仪中获得单个大分子或生物分子离子的质谱的方法。 该方法包括通过大分子或生物分子的离子化在离子阱中产生单个大分子或生物分子一级离子; 用第一电荷检测器喷射一半的初级离子用于检测; 喷射一半的初级离子以冲击转换倍增极，从而产生二次离子，以便用电荷放大检测器（例如通道电极或电倍增管或MCP）进行检测。
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公开(公告)号：US20110284733A1
公开(公告)日：2011-11-24
申请号：US12785945
申请日：2010-05-24
申请人： Chung-Hsuan Chen , Chien-Hsun Chen , Jung-Lee Lin , Ming Lee Chu
发明人： Chung-Hsuan Chen , Chien-Hsun Chen , Jung-Lee Lin , Ming Lee Chu
CPC分类号： H01J49/025 , H01J49/0027
摘要： A mass spectrometer and methods for obtaining the mass spectrum of a single macromolecular or biomolecular ion in a mass spectrometer. The methods include creating single macromolecular or biomolecular primary ions in an ion trap by ionization of a macromolecule or biomolecule; ejecting half of the primary ions for detection with a first charge detector; ejecting half of the primary ions to impact upon a conversion dynode, thereby creating secondary ions for detection with charge amplification detector such as a channeltron or an electromultiplier or an MCP.
摘要翻译： 质谱仪和在质谱仪中获得单个大分子或生物分子离子的质谱的方法。 该方法包括通过大分子或生物分子的离子化在离子阱中产生单个大分子或生物分子一级离子; 用第一电荷检测器喷射一半的初级离子用于检测; 喷射一半的初级离子以冲击转换倍增极，从而产生二次离子，以便用电荷放大检测器（例如通道电极或电倍增管或MCP）进行检测。
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公开(公告)号：US09224586B2
公开(公告)日：2015-12-29
申请号：US12976543
申请日：2010-12-22
申请人： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
发明人： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
CPC分类号： H01J49/0022 , H01J49/424 , H01J49/429
摘要： Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and/or are configured to obtain mass spectra of a large analyte, such as analyte with an m/z ratio of at least 105, or analyte with a molecular weight of at least 105 Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.
摘要翻译： 公开了便携式质谱法的方法和装置。 该装置包括至少一个离子化分析物源，至少一个频率扫描子系统，至少一个检测器和可选的至少一个真空泵，并且是便携式的。 在一些实施方案中，装置包括多个电离分析物源和/或配置成获得大分析物的质谱，例如m / z比至少为105的分析物，或分子量至少为105的分析物 Da，以及小分子分析物的质谱。 在一些实施例中，所述方法包括使用上述便携式装置获得质谱。
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公开(公告)号：US08507846B2
公开(公告)日：2013-08-13
申请号：US13566352
申请日：2012-08-03
申请人： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
发明人： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
CPC分类号： H01J49/4285 , H01J49/0031 , H01J49/424
摘要： An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by step scanning the driving frequency in frequency increments over a bandwidth, wherein for each step a specific frequency is held for a fixed number of complete cycles, wherein each specific frequency is changed continuously to the frequency in the next step, and wherein each specific frequency in each step starts at phase zero position.
摘要翻译： 一种离子阱质谱仪和用于通过在带宽上以频率增量扫描驱动频率来获得离子质谱的方法，其中对于每个步骤，特定频率保持固定数量的完整循环，其中每个特定频率被改变 在下一步骤中连续地进入频率，并且其中每个步骤中的每个特定频率从相位零位置开始。
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公开(公告)号：US20110147581A1
公开(公告)日：2011-06-23
申请号：US12976543
申请日：2010-12-22
申请人： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
发明人： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
IPC分类号： H01J49/26
CPC分类号： H01J49/0022 , H01J49/424 , H01J49/429
摘要： Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and/or are configured to obtain mass spectra of a large analyte, such as analyte with an m/z ratio of at least 105, or analyte with a molecular weight of at least 105 Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.
摘要翻译： 公开了便携式质谱法的方法和装置。 该装置包括至少一个离子化分析物源，至少一个频率扫描子系统，至少一个检测器和可选的至少一个真空泵，并且是便携式的。 在一些实施方案中，装置包括多个电离分析物源和/或配置成获得大分析物的质谱，例如m / z比至少为105的分析物，或分子量至少为105的分析物 Da，以及小分子分析物的质谱。 在一些实施例中，所述方法包括使用上述便携式装置获得质谱。
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公开(公告)号：US08344317B2
公开(公告)日：2013-01-01
申请号：US13365105
申请日：2012-02-02
申请人： Chung-Hsuan Chen , Jung-Lee Lin , Nien-Yeen Hsu , Yi-Sheng Wang
发明人： Chung-Hsuan Chen , Jung-Lee Lin , Nien-Yeen Hsu , Yi-Sheng Wang
CPC分类号： H05H5/047 , H01J49/06 , H01J49/40 , H01J49/403
摘要： A novel system and methods for accelerating analytes including, without limitation, molecular ions, biomolecules, polymers, nano- and microparticles, is provided. The invention can be useful for increasing detection sensitivity in applications such as mass spectrometry, performing collision-induced dissociation molecular structure analysis, and probing surfaces and samples using accelerated analyte.
摘要翻译： 提供了一种用于加速分析物的新系统和方法，包括但不限于分子离子，生物分子，聚合物，纳米和微粒。 本发明可用于在诸如质谱法，进行碰撞诱导的解离分子结构分析和使用加速分析物的探测表面和样品等应用中提高检测灵敏度。
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公开(公告)号：US20110195133A1
公开(公告)日：2011-08-11
申请号：US12703102
申请日：2010-02-09
IPC分类号： A61K33/24 , C07K7/06 , A61K31/7088 , A61P35/00 , C12Q1/00
CPC分类号： C07K14/4723 , A61K31/7088 , A61K33/24 , A61K38/04 , A61K45/06 , G01N30/72 , G01N33/57423 , G01N33/57488 , G01N33/6851 , A61K2300/00
摘要： Methods for diagnosis of non-small cell lung cancers by detection of endogenous peptides in exhaled breath condensate (EBC) are provided. Diagnostic peptides derived from dermcidin (DCD) are provided. A specific dermcidin-derived peptide E-R11, having the sequence ENAGEDPGLAR, is provided. E-R11 peptide levels in EBC, as measured by mass spectrometry (MS), are highly diagnostic of non-small cell lung cancers. A method for inhibiting growth of lung cancer cells by inhibiting DCD expression by RNA interference also is provided.
摘要翻译： 提供了通过检测呼出气体浓缩物（EBC）中的内源肽来诊断非小细胞肺癌的方法。 提供衍生自皮肤霉素（DCD）的诊断肽。 提供具有ENAGEDPGLAR序列的具体的皮肤霉素衍生肽E-R11。 通过质谱（MS）测量，EBC中的E-R11肽水平是非小细胞肺癌的高度诊断。 还提供了通过RNA干扰抑制DCD表达来抑制肺癌细胞生长的方法。
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公开(公告)号：US5184015A
公开(公告)日：1993-02-02
申请号：US766542
申请日：1991-09-27
申请人： Steve L. Allman , Chung-Hsuan Chen , Fang C. Chen
发明人： Steve L. Allman , Chung-Hsuan Chen , Fang C. Chen
IPC分类号： G01N27/64
CPC分类号： G01N27/622
摘要： A method for analyzing a gaseous electronegative species comprises the steps of providing an analysis chamber; providing an electric field of known potential within the analysis chamber; admitting into the analysis chamber a gaseous sample containing the gaseous electronegative species; providing a pulse of free electrons within the electric field so that the pulse of free electrons interacts with the gaseous electronegative species so that a swarm of electrically charged particles is produced within the electric field; and, measuring the mobility of the electrically charged particles within the electric field.
摘要翻译： 用于分析气体电负性物质的方法包括提供分析室的步骤; 在分析室内提供已知电位的电场; 进入分析室中含有气态电负性物质的气态样品; 在电场内提供自由电子脉冲，使得自由电子的脉冲与气体电负性物质相互作用，从而在电场内产生带电粒子群; 并测量电场内的带电粒子的迁移率。
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公开(公告)号：US09458555B2
公开(公告)日：2016-10-04
申请号：US12687044
申请日：2010-01-13
申请人： Chung-Hsuan Chen , Chen-Ren Hsiao
发明人： Chung-Hsuan Chen , Chen-Ren Hsiao
IPC分类号： C12M1/34 , C12M3/00 , C12Q1/68 , G01N33/53 , C12P19/34 , C07H21/02 , C07H21/04 , A61K38/00 , C07K1/00 , C40B30/04 , G01N33/58
CPC分类号： C40B30/04 , G01N33/587
摘要： Methods for determining the quality of a biomolecular microarray to determine suitability of the microarray for performing specific binding reactions, such as hybridization, are provided. Methods are based on staining a microarray with a solution of detectable nanoparticles that reversibly stain the biomolecules through an electrostatic interaction to select microarrays that meet quality standards for hybridization reactions. A gold nanoparticle solution based staining method for DNA microarrays is provided. Destaining methods allowing multiple rounds of hybridization of nanogold stained microarrays are provided. Microarrays selected by methods of the invention are provided.
摘要翻译： 提供了用于确定生物分子微阵列的质量以确定用于进行特异性结合反应（例如杂交）的微阵列适合性的方法。 方法是基于用可检测纳米颗粒的溶液染色微阵列，其通过静电相互作用可逆地染色生物分子，以选择满足杂交反应质量标准的微阵列。 提供了一种基于金纳米颗粒溶液的DNA微阵列染色方法。 提供了允许纳米金染色的微阵列进行多轮杂交的脱色方法。 提供了通过本发明的方法选择的微阵列。
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公开(公告)号：US06309451B1
公开(公告)日：2001-10-30
申请号：US09491773
申请日：2000-01-26
申请人： Chung-Hsuan Chen
发明人： Chung-Hsuan Chen
IPC分类号： B01D5304
CPC分类号： B01D46/0036 , B01D46/10 , B01D53/0407 , B01D2253/102 , B01D2259/4566 , B01D2275/10 , B01D2279/60
摘要： An air filtering device includes an inner seat member, an outer seat member, and a multi-layer filter medium. The inner seat member has opposed first and second sides and a plurality of first air passages extending therethrough from the first side to the second side. The outer seat member has opposed third and fourth sides and a plurality of second air passages extending therethrough from the third side to the fourth side. The multi-layer filter medium is sandwiched between the second side of the inner seat member and the third side of the outer seat member. The multi-layer filter medium has a polypropylene fabric layer, a far-infrared radiation material layer, and an activated carbon fiber layer connected to one another. The first air passages are registered with the second air passages.
摘要翻译： 一种空气过滤装置，包括内座椅构件，外座椅构件和多层过滤介质。 内座椅构件具有相对的第一和第二侧面以及从第一侧向第二侧延伸穿过的多个第一空气通道。 外座构件具有相对的第三和第四侧面以及从第三侧向第四侧延伸穿过的多个第二空气通道。 多层过滤介质被夹在内座构件的第二侧和外座构件的第三侧之间。 多层过滤介质具有聚丙烯织物层，远红外辐射材料层和彼此连接的活性碳纤维层。 第一空气通道与第二空气通道对准。
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