公开(公告)号：US20110195133A1

公开(公告)日：2011-08-11

申请号：US12703102

申请日：2010-02-09

Applicant: Chung-Hsuan Chen ,  Wei-Chao Chang ,  Michael Hsiao ,  Ming-Shyan Huang ,  Chih-Jen Yang

Inventor： Chung-Hsuan Chen ,  Wei-Chao Chang ,  Michael Hsiao ,  Ming-Shyan Huang ,  Chih-Jen Yang

IPC: A61K33/24 ,  C07K7/06 ,  A61K31/7088 ,  A61P35/00 ,  C12Q1/00

CPC classification number: C07K14/4723 ,  A61K31/7088 ,  A61K33/24 ,  A61K38/04 ,  A61K45/06 ,  G01N30/72 ,  G01N33/57423 ,  G01N33/57488 ,  G01N33/6851 ,  A61K2300/00

Abstract: Methods for diagnosis of non-small cell lung cancers by detection of endogenous peptides in exhaled breath condensate (EBC) are provided. Diagnostic peptides derived from dermcidin (DCD) are provided. A specific dermcidin-derived peptide E-R11, having the sequence ENAGEDPGLAR, is provided. E-R11 peptide levels in EBC, as measured by mass spectrometry (MS), are highly diagnostic of non-small cell lung cancers. A method for inhibiting growth of lung cancer cells by inhibiting DCD expression by RNA interference also is provided.

Abstract translation: 提供了通过检测呼出气体浓缩物（EBC）中的内源肽来诊断非小细胞肺癌的方法。 提供衍生自皮肤霉素（DCD）的诊断肽。 提供具有ENAGEDPGLAR序列的具体的皮肤霉素衍生肽E-R11。 通过质谱（MS）测量，EBC中的E-R11肽水平是非小细胞肺癌的高度诊断。 还提供了通过RNA干扰抑制DCD表达来抑制肺癌细胞生长的方法。

公开(公告)号：US5184015A

公开(公告)日：1993-02-02

申请号：US766542

申请日：1991-09-27

Applicant: Steve L. Allman ,  Chung-Hsuan Chen ,  Fang C. Chen

Inventor： Steve L. Allman ,  Chung-Hsuan Chen ,  Fang C. Chen

IPC: G01N27/64

CPC classification number: G01N27/622

Abstract: A method for analyzing a gaseous electronegative species comprises the steps of providing an analysis chamber; providing an electric field of known potential within the analysis chamber; admitting into the analysis chamber a gaseous sample containing the gaseous electronegative species; providing a pulse of free electrons within the electric field so that the pulse of free electrons interacts with the gaseous electronegative species so that a swarm of electrically charged particles is produced within the electric field; and, measuring the mobility of the electrically charged particles within the electric field.

Abstract translation: 用于分析气体电负性物质的方法包括提供分析室的步骤; 在分析室内提供已知电位的电场; 进入分析室中含有气态电负性物质的气态样品; 在电场内提供自由电子脉冲，使得自由电子的脉冲与气体电负性物质相互作用，从而在电场内产生带电粒子群; 并测量电场内的带电粒子的迁移率。

公开(公告)号：US09458555B2

公开(公告)日：2016-10-04

申请号：US12687044

申请日：2010-01-13

Applicant: Chung-Hsuan Chen ,  Chen-Ren Hsiao

Inventor： Chung-Hsuan Chen ,  Chen-Ren Hsiao

IPC: C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  G01N33/53 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  A61K38/00 ,  C07K1/00 ,  C40B30/04 ,  G01N33/58

CPC classification number: C40B30/04 ,  G01N33/587

Abstract: Methods for determining the quality of a biomolecular microarray to determine suitability of the microarray for performing specific binding reactions, such as hybridization, are provided. Methods are based on staining a microarray with a solution of detectable nanoparticles that reversibly stain the biomolecules through an electrostatic interaction to select microarrays that meet quality standards for hybridization reactions. A gold nanoparticle solution based staining method for DNA microarrays is provided. Destaining methods allowing multiple rounds of hybridization of nanogold stained microarrays are provided. Microarrays selected by methods of the invention are provided.

Abstract translation: 提供了用于确定生物分子微阵列的质量以确定用于进行特异性结合反应（例如杂交）的微阵列适合性的方法。 方法是基于用可检测纳米颗粒的溶液染色微阵列，其通过静电相互作用可逆地染色生物分子，以选择满足杂交反应质量标准的微阵列。 提供了一种基于金纳米颗粒溶液的DNA微阵列染色方法。 提供了允许纳米金染色的微阵列进行多轮杂交的脱色方法。 提供了通过本发明的方法选择的微阵列。

公开(公告)号：US09224586B2

公开(公告)日：2015-12-29

申请号：US12976543

申请日：2010-12-22

Applicant: Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

Inventor： Chung-Hsuan Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

IPC: H01J49/42 ,  H01J49/00

CPC classification number: H01J49/0022 ,  H01J49/424 ,  H01J49/429

Abstract: Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and/or are configured to obtain mass spectra of a large analyte, such as analyte with an m/z ratio of at least 105, or analyte with a molecular weight of at least 105 Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.

Abstract translation: 公开了便携式质谱法的方法和装置。 该装置包括至少一个离子化分析物源，至少一个频率扫描子系统，至少一个检测器和可选的至少一个真空泵，并且是便携式的。 在一些实施方案中，装置包括多个电离分析物源和/或配置成获得大分析物的质谱，例如m / z比至少为105的分析物，或分子量至少为105的分析物 Da，以及小分子分析物的质谱。 在一些实施例中，所述方法包括使用上述便携式装置获得质谱。

公开(公告)号：US08344317B2

公开(公告)日：2013-01-01

申请号：US13365105

申请日：2012-02-02

Applicant: Chung-Hsuan Chen ,  Jung-Lee Lin ,  Nien-Yeen Hsu ,  Yi-Sheng Wang

Inventor： Chung-Hsuan Chen ,  Jung-Lee Lin ,  Nien-Yeen Hsu ,  Yi-Sheng Wang

IPC: H01J49/26 ,  H01J49/40

CPC classification number: H05H5/047 ,  H01J49/06 ,  H01J49/40 ,  H01J49/403

Abstract: A novel system and methods for accelerating analytes including, without limitation, molecular ions, biomolecules, polymers, nano- and microparticles, is provided. The invention can be useful for increasing detection sensitivity in applications such as mass spectrometry, performing collision-induced dissociation molecular structure analysis, and probing surfaces and samples using accelerated analyte.

Abstract translation: 提供了一种用于加速分析物的新系统和方法，包括但不限于分子离子，生物分子，聚合物，纳米和微粒。 本发明可用于在诸如质谱法，进行碰撞诱导的解离分子结构分析和使用加速分析物的探测表面和样品等应用中提高检测灵敏度。

公开(公告)号：US06309451B1

公开(公告)日：2001-10-30

申请号：US09491773

申请日：2000-01-26

Applicant: Chung-Hsuan Chen

Inventor： Chung-Hsuan Chen

IPC: B01D5304

CPC classification number: B01D46/0036 ,  B01D46/10 ,  B01D53/0407 ,  B01D2253/102 ,  B01D2259/4566 ,  B01D2275/10 ,  B01D2279/60

Abstract: An air filtering device includes an inner seat member, an outer seat member, and a multi-layer filter medium. The inner seat member has opposed first and second sides and a plurality of first air passages extending therethrough from the first side to the second side. The outer seat member has opposed third and fourth sides and a plurality of second air passages extending therethrough from the third side to the fourth side. The multi-layer filter medium is sandwiched between the second side of the inner seat member and the third side of the outer seat member. The multi-layer filter medium has a polypropylene fabric layer, a far-infrared radiation material layer, and an activated carbon fiber layer connected to one another. The first air passages are registered with the second air passages.

Abstract translation: 一种空气过滤装置，包括内座椅构件，外座椅构件和多层过滤介质。 内座椅构件具有相对的第一和第二侧面以及从第一侧向第二侧延伸穿过的多个第一空气通道。 外座构件具有相对的第三和第四侧面以及从第三侧向第四侧延伸穿过的多个第二空气通道。 多层过滤介质被夹在内座构件的第二侧和外座构件的第三侧之间。 多层过滤介质具有聚丙烯织物层，远红外辐射材料层和彼此连接的活性碳纤维层。 第一空气通道与第二空气通道对准。

公开(公告)号：US08309913B2

公开(公告)日：2012-11-13

申请号：US12689506

申请日：2010-01-19

Applicant: Yi-Sheng Wang ,  Chung-Hsuan Chen

Inventor： Yi-Sheng Wang ,  Chung-Hsuan Chen

IPC: H01J49/10

CPC classification number: H01J49/40 ,  H01J49/0095

Abstract: An angled dual-polarity mass spectrometer includes a dual-polarity ion generator, a first mass analyzer, and a second mass analyzer. The dual-polarity ion generator includes an ion source to generate positive ions and negative ions from a sample, and electrodes to generate electric fields for guiding the negative ions into a beam of negative ions and guiding the positive ions into a beam of positive ions. The first mass analyzer can analyze the negative ions, and the second mass analyzer can analyze the positive ions. The central axes of the first and the second mass analyzers are at an angle between 0 to 179 degrees.

Abstract translation: 角度双极性质谱仪包括双极性离子发生器，第一质量分析器和第二质量分析器。 双极性离子发生器包括从样品产生正离子和负离子的离子源，以及用于产生电场的电极，用于将负离子引导到负离子束中并将正离子引导到正离子束中。 第一个质谱仪可以分析负离子，第二个质谱仪可以分析正离子。 第一和第二质量分析器的中心轴线在0至179度之间的角度。

公开(公告)号：US08258464B2

公开(公告)日：2012-09-04

申请号：US12785945

申请日：2010-05-24

Applicant: Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

Inventor： Chung-Hsuan Chen ,  Chien-Hsun Chen ,  Jung-Lee Lin ,  Ming-Lee Chu

IPC: H01J49/34 ,  H01J37/244

CPC classification number: H01J49/025 ,  H01J49/0027

Abstract: A mass spectrometer and methods for obtaining the mass spectrum of a single macromolecular or biomolecular ion in a mass spectrometer. The methods include creating single macromolecular or biomolecular primary ions in an ion trap by ionization of a macromolecule or biomolecule; ejecting half of the primary ions for detection with a first charge detector; ejecting half of the primary ions to impact upon a conversion dynode, thereby creating secondary ions for detection with charge amplification detector such as a channeltron or an electromultiplier or an MCP.

Abstract translation: 质谱仪和在质谱仪中获得单个大分子或生物分子离子的质谱的方法。 该方法包括通过大分子或生物分子的离子化在离子阱中产生单个大分子或生物分子一级离子; 用第一电荷检测器喷射一半的初级离子用于检测; 喷射一半的初级离子以冲击转换倍增极，从而产生二次离子，以便用电荷放大检测器（例如通道电极或电倍增管或MCP）进行检测。

公开(公告)号：US07649170B2

公开(公告)日：2010-01-19

申请号：US11542568

申请日：2006-10-03

Applicant: Yi-Sheng Wang ,  Chung-Hsuan Chen ,  Shang-Ting Tsai ,  Chiu Wen Chen

Inventor： Yi-Sheng Wang ,  Chung-Hsuan Chen ,  Shang-Ting Tsai ,  Chiu Wen Chen

IPC: H01J49/10

CPC classification number: H01J49/40 ,  H01J49/0095

Abstract: A dual-polarity mass spectrometer includes an ion source, a negative ion mass analyzer, and a positive ion mass analyzer to measure both the negative and positive ion spectra of a sample material simultaneously. The ion source includes a sample surface on which the sample material is positioned, the sample material providing positive ions and negative ions when excited by a laser beam or an energetic particle stream. A first extraction electrode is connected to a voltage higher than the sample surface to attract the negative ions from the sample electrode. A second extraction electrode is connected to a voltage lower than the sample surface to attract the positive ions from the sample electrode. The negative and positive ions are analyzed simultaneously by the negative ion mass analyzer and the positive ion mass analyzer, respectively.

Abstract translation: 双极性质谱仪包括离子源，负离子质量分析仪和正离子质量分析仪，以同时测量样品材料的负离子和正离子光谱。 离子源包括其上放置样品材料的样品表面，当由激光束或能量粒子流激发时，样品材料提供正离子和负离子。 第一引出电极连接到高于样品表面的电压以吸引来自样品电极的负离子。 第二提取电极连接到低于样品表面的电压，以吸引来自样品电极的正离子。 负离子和正离子分别通过负离子质量分析仪和正离子质量分析仪同时分析。

公开(公告)号：US4426576A

公开(公告)日：1984-01-17

申请号：US300019

申请日：1981-09-08

Applicant: G. Samuel Hurst ,  Marvin G. Payne ,  Chung-Hsuan Chen ,  James E. Parks

Inventor： G. Samuel Hurst ,  Marvin G. Payne ,  Chung-Hsuan Chen ,  James E. Parks

IPC: H01J49/02 ,  H01J49/04 ,  H01J49/16 ,  H01J49/42 ,  B01D59/44

CPC classification number: H01J49/162 ,  H01J49/0422

Abstract: Apparatus and methods of operation are described for determining, with isotopic selectivity, the number of noble gas atoms in a sample. The analysis is conducted within an evacuated chamber which can be isolated by a valve from a vacuum pumping system capable of producing a pressure of 10.sup.-8 Torr. Provision is made to pass pulses of laser beams through the chamber, these pulses having wavelengths appropriate for the resonance ionization of atoms of the noble gas under analysis. A mass filter within the chamber selects ions of a specific isotope of the noble gas, and means are provided to accelerate these selected ions sufficiently for implantation into a target. Specific types of targets are discussed. An electron measuring device produces a signal relatable to the number of ions implanted into the target and thus to the number of atoms of the selected isotope of the noble gas removed from the gas sample. The measurement can be continued until a substantial fraction, or all, of the atoms in the sample have been counted.Furthermore, additional embodiments of the apparatus are described for bunching the atoms of a noble gas for more rapid analysis, and for changing the target for repetitive cycling of the gas in the chamber. The number of repetitions of the cyclic steps depend upon the concentration of the isotope of interest, the separative efficiency of the mass filter, etc. The cycles are continued until a desired selectivity is achieved. Also described are components and a method of operation for a pre-enrichment operation for use when an introduction of a total sample would elevate the pressure within the chamber to levels in excess of those for operation of the mass filter, specifically a quadrupole mass filter.Specific examples of three noble gas isotope analyses are described.

Abstract translation: 描述了操作的装置和方法，以同位素选择性来确定样品中惰性气体原子的数量。 该分析在真空室内进行，其可以通过阀从能够产生10-8托的压力的真空泵系统隔离。 提供了将激光束的脉冲通过腔室，这些脉冲具有适于分析的惰性气体的原子的共振电离的波长。 室内的质量过滤器选择惰性气体的特定同位素的离子，并且提供了用于将这些选定的离子充分加速以植入靶中的装置。 讨论具体类型的目标。 电子测量装置产生与植入靶中的离子数相关的信号，从而产生从气体样品中除去的惰性气体的选定同位素原子数。 可以继续测量，直到已经计数了样品中的大部分原子或全部原子。 此外，描述了该设备的附加实施例，用于聚合惰性气体的原子以进行更快速的分析，并且用于改变靶室以使腔室中的气体重复循环。 循环步骤的重复次数取决于所关心的同位素的浓度，质量过滤器的分离效率等。循环持续到达到期望的选择性。 还描述了当全部样品的引入将室内的压力升高到超过用于质量过滤器，特别是四极质量过滤器的操作的水平时，用于预浓缩操作的组件和操作方法。 描述了三种惰性气体同位素分析的具体实例。