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    Use and production of storage-stable neutral metalloprotease
    2.
    发明授权
    Use and production of storage-stable neutral metalloprotease 有权

    公开(公告)号：US11091750B2

    公开(公告)日：2021-08-17

    申请号：US16668635

    申请日：2019-10-30

    Applicant: DANISCO US INC

    Inventor： Andrew Shaw ,  David A Estell ,  Eugene S Sadlowski ,  Hiroshi Oh ,  Louise Wallace ,  Ronaldus Wilhelmus Joannes Hommes ,  Sang-Kyu Lee

    IPC: C12N9/54 ,  C11D3/386 ,  C11D1/02

    Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

    USE AND PRODUCTION OF STORAGE-STABLE NEUTRAL METALLOPROTEASE
    3.
    发明申请
    USE AND PRODUCTION OF STORAGE-STABLE NEUTRAL METALLOPROTEASE 审中-公开
    使用和生产储存稳定的中性金属蛋白酶

    公开(公告)号：US20170022490A1

    公开(公告)日：2017-01-26

    申请号：US15094761

    申请日：2016-04-08

    Applicant: Danisco US Inc.

    Inventor： Andrew Shaw ,  Louise Wallace ,  David A. Estell ,  Ronaldus W.J. Hommes ,  Sang-Kyu Lee ,  Hiroshi Oh ,  Eugene S. Sadlowski

    IPC: C12N9/54 ,  C11D1/02 ,  C11D3/386

    CPC classification number: C12N9/54 ,  C11D1/02 ,  C11D3/386 ,  C11D3/38663 ,  C12Y304/24

    Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

    Abstract translation: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。

    USE AND PRODUCTION OF STORAGE-STABLE NEUTRAL METALLOPROTEASE
    5.
    发明申请
    USE AND PRODUCTION OF STORAGE-STABLE NEUTRAL METALLOPROTEASE 有权
    使用和生产储存稳定的中性金属蛋白酶

    公开(公告)号：US20140134708A1

    公开(公告)日：2014-05-15

    申请号：US14037883

    申请日：2013-09-26

    Applicant: Danisco US Inc.

    Inventor： Andrew Shaw ,  Louise Wallace ,  David A. Estell ,  Ronaldus W.J. Hommes ,  Sang-Kyu Lee ,  Hiroshi Oh ,  Eugene S. Sadlowski

    IPC: C11D3/386 ,  C12N9/54

    CPC classification number: C12N9/54 ,  C11D1/02 ,  C11D3/386 ,  C11D3/38663 ,  C12Y304/24

    Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

    Abstract translation: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。

    Use and production of storage-stable neutral metalloprotease
    6.
    发明授权
    Use and production of storage-stable neutral metalloprotease 有权
    使用和生产储存稳定的中性金属蛋白酶

    公开(公告)号：US09334467B2

    公开(公告)日：2016-05-10

    申请号：US14037883

    申请日：2013-09-26

    Applicant: Danisco US Inc.

    Inventor： Andrew Shaw ,  Louise Wallace ,  David A. Estell ,  Ronaldus W. J. Hommes ,  Sang-Kyu Lee ,  Hiroshi Oh ,  Eugene S. Sadlowski

    IPC: C12N9/54 ,  C12N9/50 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/20 ,  C11D3/00 ,  C07H21/04 ,  C07K1/00 ,  C11D3/386

    CPC classification number: C12N9/54 ,  C11D1/02 ,  C11D3/386 ,  C11D3/38663 ,  C12Y304/24

    Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

    Abstract translation: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中，本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中，中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中，中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁，漂白和消毒的应用。

    Multiple mutation variants of serine protease
    8.
    发明授权
    Multiple mutation variants of serine protease 有权
    丝氨酸蛋白酶的多重突变变体

    公开(公告)号：US08865449B2

    公开(公告)日：2014-10-21

    申请号：US13888300

    申请日：2013-05-06

    Applicant: Danisco US Inc.

    Inventor： Wolfgang Aehle ,  David A. Estell ,  Ronaldus W. J. Hommes ,  Brian E. Jones ,  Marc Kolkman ,  Chris Leeflang ,  Hiroshi Oh ,  Ayrookaran J. Poulose ,  Andrew Shaw ,  Wilhelmus A. H. Van der Kley ,  Leonardus P. M. Van Marrewijk

    IPC: C12N9/52 ,  A23K1/165 ,  A23K1/18 ,  C11D3/386 ,  D06M16/00

    CPC classification number: C12N9/52 ,  A23K20/189 ,  A23K50/75 ,  C11D3/386 ,  C12N9/96 ,  C12Y304/21 ,  D06M16/003

    Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

    Abstract translation: 本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。 特别地，本发明提供了具有多个取代的丝氨酸蛋白酶，编码这些蛋白酶的DNA，包含编码蛋白酶的DNA的载体，用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物（例如洗涤剂组合物），动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。 在特别优选的实施方案中，本发明提供衍生自本文所述的野生型蛋白酶的突变体（即，变体）蛋白酶。 这些变体蛋白酶也可用于许多应用。

    MULTIPLE MUTATION VARIANTS OF SERINE PROTEASE
    9.
    发明申请
    MULTIPLE MUTATION VARIANTS OF SERINE PROTEASE 有权
    丝氨酸蛋白酶的多个突变变体

    公开(公告)号：US20140005094A1

    公开(公告)日：2014-01-02

    申请号：US13888300

    申请日：2013-05-06

    Applicant: Danisco US Inc.

    Inventor： Wolfgang Aehle ,  David A. Estell ,  Ronaldus W.J. Hommes ,  Brian E. Jones ,  Marc Kolkman ,  Chris Leeflang ,  Hiroshi Oh ,  Ayrookaran J. Poulose ,  Andrew Shaw ,  Wilhelmus A.H. Van der Kley ,  Leonardus P.M. Van Marrewijk

    IPC: C12N9/52

    CPC classification number: C12N9/52 ,  A23K20/189 ,  A23K50/75 ,  C11D3/386 ,  C12N9/96 ,  C12Y304/21 ,  D06M16/003

    Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

    Abstract translation: 本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。 特别地，本发明提供了具有多个取代的丝氨酸蛋白酶，编码这些蛋白酶的DNA，包含编码蛋白酶的DNA的载体，用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物（例如洗涤剂组合物），动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。 在特别优选的实施方案中，本发明提供衍生自本文所述的野生型蛋白酶的突变体（即，变体）蛋白酶。 这些变体蛋白酶也可用于许多应用。

    Use and production of storage-stable neutral metalloprotease
    10.
    发明授权
    Use and production of storage-stable neutral metalloprotease 有权

    公开(公告)号：US10577595B2

    公开(公告)日：2020-03-03

    申请号：US15788079

    申请日：2017-10-19

    Applicant: DANISCO US INC

    Inventor： Andrew Shaw ,  David A Estell ,  Eugene S Sadlowski ,  Hiroshi Oh ,  Louise Wallace ,  Ronaldus Wilhelmus Joannes Hommes ,  Sang-Kyu Lee

    IPC: C12N9/54 ,  C11D1/02 ,  C11D3/386

    Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

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