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    Systems and methods for nucleic acid sequencing
    1.
    发明授权
    Systems and methods for nucleic acid sequencing 有权

    公开(公告)号：US09797010B2

    公开(公告)日：2017-10-24

    申请号：US12809120

    申请日：2008-12-19

    申请人： David A. Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

    发明人： David A. Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q2537/143 ,  C12Q2563/149

    摘要： The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.

    SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
    2.
    发明申请
    SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING 有权
    核酸序列的系统和方法

    公开(公告)号：US20110267457A1

    公开(公告)日：2011-11-03

    申请号：US12809120

    申请日：2008-12-19

    申请人： David A Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

    发明人： David A Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

    IPC分类号： C40B30/04 ,  C07H1/00 ,  H04N7/18 ,  G01N33/53

    CPC分类号： C12Q1/6874 ,  C12Q2537/143 ,  C12Q2563/149

    摘要： The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.

    摘要翻译： 本发明涉及用于测序核酸的系统和方法，包括以流体液滴测序核酸。 在一组实施方案中，该方法采用使用微滴（例如微流控液滴）的杂交进行测序。 在一些实施方案中，形成液滴，其包括靶核酸，核酸探针和至少一种识别元件，例如荧光颗粒。 在某些情况下，通过确定至少一个识别元件来确定与靶核酸杂交的核酸探针。 与靶核酸杂交的核酸探针可用于测定靶核酸的序列。 在某些情况下，向微流体液滴提供修饰核酸探针的试剂。 在一些情况下，诸如上述那些的液滴变形，使得液滴的组分分别通过目标区域。

    FLUID INJECTION
    4.
    发明申请
    FLUID INJECTION 有权
    流体注射

    公开(公告)号：US20120132288A1

    公开(公告)日：2012-05-31

    申请号：US13379782

    申请日：2010-06-25

    申请人： David A. Weitz ,  Adam R. Abate ,  Tony Hung ,  Pascaline Mary

    发明人： David A. Weitz ,  Adam R. Abate ,  Tony Hung ,  Pascaline Mary

    IPC分类号： F15C1/00

    CPC分类号： B01F5/0471 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/00862 ,  B01J2219/00889 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2300/0867 ,  B01L2400/0415 ,  F16K99/0042 ,  F16K99/0051 ,  G01N27/44791 ,  G01N2035/1034 ,  Y10T137/0391 ,  Y10T137/206

    摘要： The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

    摘要翻译： 本发明一般涉及用于控制流体的系统和方法，并且在一些情况下涉及将流体流入和/或流出其它流体的系统和方法。 作为示例，流体可以被注入到包含在流体通道内的液滴中，或者可以将流体注入到流体通道中以产生液滴。 在一些实施例中，可以使用电极将电场施加到一个或多个流体通道，例如至少两个流体通道的相交处。 例如，可以通过电场促进第一流体进入和/或流出第二流体。 在一些情况下，电场可能破坏第一流体和至少一种其它流体之间的界面。 可以通过控制流体和/或流体液滴的各种性质（例如流体液滴的曲率）来控制被推入和/或流出第二流体的第一流体的体积，流速等的性质 ，和/或控制所施加的电场。

    Digital golf score device
    9.
    外观设计
    Digital golf score device 失效
    数位高尔夫评分装置

    公开(公告)号：USD467510S1

    公开(公告)日：2002-12-24

    申请号：US29155355

    申请日：2002-02-05

    申请人： Tony Hung

    设计人： Tony Hung