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    Purified thermostable enzyme
    1.
    发明授权
    Purified thermostable enzyme 失效
    纯化热稳定酶

    公开(公告)号：US4889818A

    公开(公告)日：1989-12-26

    申请号：US063509

    申请日：1987-06-17

    申请人： David H. Gelfand ,  Susanne Stoffel ,  Frances C. Lawyer ,  Randall K. Saiki

    发明人： David H. Gelfand ,  Susanne Stoffel ,  Frances C. Lawyer ,  Randall K. Saiki

    IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12N9/1276

    摘要： A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.

    摘要翻译： 获得了具有独特特征的纯化的热稳定酶。 优选地，酶从水生栖热菌属物种分离并且具有约86,000-90,000道尔顿的分子量。 热稳定酶可以是天然的或重组的，并且可以用于温度循环链式反应，其中借助于所选择的引物和三磷酸核苷酸，从现有序列中至少一个核酸序列的量被扩增。 该酶优选储存在提供酶稳定性的非离子型洗涤剂的缓冲液中。

    Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
    2.
    发明授权
    Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents 失效
    含有非离子聚合物洗涤剂的稳定的耐热核酸聚合酶组合物

    公开(公告)号：US6127155A

    公开(公告)日：2000-10-03

    申请号：US873897

    申请日：1992-04-24

    申请人： David H. Gelfand ,  Susanne Stoffel ,  Randall K. Saiki

    发明人： David H. Gelfand ,  Susanne Stoffel ,  Randall K. Saiki

    IPC分类号： C12N9/12 ,  C12N15/70 ,  C12P19/34 ,  C12Q1/68 ,  C12N9/96 ,  C12N9/00 ,  C12P21/06

    CPC分类号： C12P19/34 ,  C12N15/70 ,  C12N9/1252 ,  C12Q1/686 ,  Y10S435/814

    摘要： A purified thermostable nucleic acid polymerase is obtained that has unique characteristics. Preferably the nucleic acid polymerase is DNA polymerase isolated from a Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable nucleic acid polymerase may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The nucleic acid polymerase is preferably stored in a buffer containing non-ionic detergents that lends stability to the nucleic acid polymerase. A preferred buffer contains glycerol, polyoxyethylated sorbitan monolaurate, ethoxylated nonyl phenol and gelatin.

    摘要翻译： 获得了具有独特特征的纯化的热稳定性核酸聚合酶。 优选地，所述核酸聚合酶是从栖热栖热菌属物种分离的DNA聚合酶，其分子量约为86,000-95,000道尔顿。 热稳定性核酸聚合酶可以是天然的或重组的，并且可以用于温度循环链式反应，其中借助于选定的引物和三磷酸核苷酸，从现存的序列中扩增至少一个核酸序列。 核酸聚合酶优选储存在含有使核酸聚合酶具有稳定性的非离子型清净剂的缓冲液中。 优选的缓冲液含有甘油，聚氧乙基化脱水山梨糖醇单月桂酸酯，乙氧基化壬基苯酚和明胶。