公开(公告)号：US08541220B2

公开(公告)日：2013-09-24

申请号：US12810067

申请日：2008-12-22

申请人： David P. Weiner ,  Ellen G. Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin M. Cuenca ,  Mervyn L. De Souza ,  Sherry Kollmann

发明人： David P. Weiner ,  Ellen G. Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin M. Cuenca ,  Mervyn L. De Souza ,  Sherry Kollmann

IPC分类号： C12N9/90 ,  C12N9/88 ,  C12N9/48 ,  C12N9/42 ,  C12N9/16 ,  C02F3/34 ,  A62D3/00 ,  C07H21/04 ,  C07K1/00 ,  A21D2/00 ,  A23L1/10 ,  D21C3/20

CPC分类号： C12N9/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/10 ,  A23G3/366 ,  A23G4/123 ,  A23G4/14 ,  A23K20/189 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  A61K38/52 ,  C12Q1/533 ,  C12Y501/00 ,  C12Y501/01

摘要： This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids. Thus, the invention provides enzymes, compositions, methods for production of pharmaceutical compositions, pharmaceutical intermediates, antibiotics, sweeteners, peptide enzymes, peptide hormones, fuel and fuel additive compositions, foods and food additives, beverage and beverage additives, feeds and feed additives, drugs and drug additives, dietary supplements, textiles, wood, paper, pulp, and detergents comprising the polypeptides or polynucleotides in accordance with the invention.

摘要翻译： 本发明一般涉及酶，编码酶的多核苷酸，这种多核苷酸和多肽的用途，更具体地涉及具有异构酶活性的酶，例如消旋酶活性，例如氨基酸消旋酶活性，丙氨酸消旋酶活性和/或差向异构酶活性， 和/或催化分子内原子的重新排列，催化一种异构体转化为另一种异构体，催化光学活性底物转化为光学无活性的外消旋体，催化底物对映异构体的相互转化，催化立体化学 在仅具有一个不对称中心的底物中的不对称碳原子周围反转，催化具有多于一个不对称中心的底物中不对称碳原子周围的构型的立体化学反转，和/或催化氨基酸的外消旋化。 因此，本发明提供了用于生产药物组合物，药物中间体，抗生素，甜味剂，肽酶，肽激素，燃料和燃料添加剂组合物，食品和食品添加剂，饮料和饮料添加剂，饲料和饲料添加剂的酶，组合物， 药物和药物添加剂，膳食补充剂，纺织品，木材，纸，纸浆和洗涤剂，其包含根据本发明的多肽或多核苷酸。

公开(公告)号：US20140223602A1

公开(公告)日：2014-08-07

申请号：US14039968

申请日：2013-09-27

申请人： Cathy Chang ,  Justin T. Stege ,  Nahla M. Aboushadi ,  Gordana Djordjevic ,  Ellen G. Burke ,  Peter Luginbuhl ,  Mark Dycaico ,  Toby H. Richardson ,  John Poland ,  Hefner Ying ,  Stacy M. Miles

发明人： Cathy Chang ,  Justin T. Stege ,  Nahla M. Aboushadi ,  Gordana Djordjevic ,  Ellen G. Burke ,  Peter Luginbuhl ,  Mark Dycaico ,  Toby H. Richardson ,  John Poland ,  Hefner Ying ,  Stacy M. Miles

IPC分类号： C12P19/14 ,  C12P19/02 ,  C12P7/04 ,  C12N9/42

CPC分类号： C12P19/14 ,  C12N9/2437 ,  C12N9/2445 ,  C12P7/04 ,  C12P19/02 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a β-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention.

摘要翻译： 本发明提供具有木质纤维素分解活性的多肽，例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，木糖苷酶（例如，α-氧化酶），阿拉伯呋喃糖苷酶， 和/或葡萄糖氧化酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了可以酶促加工（水解）甘蔗渣，即甘蔗渣降解或用于生物量加工的多肽，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 在一个实施方案中，本发明提供本发明的多肽的耐热性和耐热性形式。

公开(公告)号：US08343747B2

公开(公告)日：2013-01-01

申请号：US12520523

申请日：2007-12-21

申请人： Ellen G. Burke ,  Michelle H. Cayouette ,  Cathy Chang ,  Majid Ghassemian ,  Uvini Gunavardena ,  Geoff Hazlewood ,  Peter Luginbuhl ,  Aron Silverston ,  Malgorzata Slupska ,  Yan Zhang

发明人： Ellen G. Burke ,  Michelle H. Cayouette ,  Cathy Chang ,  Majid Ghassemian ,  Uvini Gunavardena ,  Geoff Hazlewood ,  Peter Luginbuhl ,  Aron Silverston ,  Malgorzata Slupska ,  Yan Zhang

IPC分类号： C12N9/34 ,  C12N9/26 ,  C12N15/00 ,  C12N1/20 ,  C12N15/82 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12N9/96 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2425 ,  C12N9/2428 ,  Y02E50/16 ,  Y02E50/17

摘要： In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

摘要翻译： 一方面，本发明涉及具有淀粉酶和/或葡糖淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化多糖，寡糖或淀粉水解成糖。 一方面，本发明提供包含通过胶乳聚合物涂层涂覆的所需成分的延迟释放组合物。 在替代实施方案中，酶用于制备生物燃料，例如乙醇，丁醇，丙醇或汽油 - 乙醇混合物，包括生物乙醇，生物丙醇，生物丁醇或生物柴油，或用于任何形式的燃料或生物质处理。