公开(公告)号：US4904581A

公开(公告)日：1990-02-27

申请号：US877609

申请日：1986-06-23

申请人： Denis R. Burger ,  Andrew S. Goldstein

发明人： Denis R. Burger ,  Andrew S. Goldstein

IPC分类号： C07K16/10

CPC分类号： C07K16/1045 ,  Y10S435/974 ,  Y10S530/808 ,  Y10S530/809 ,  Y10S530/864

摘要： A method is disclosed for detecting the presence of HTLV III infected cells in a medium. The method comprises contacting the medium with monoclonal antibodies against an antigen produced as a result of the infection and detecting the binding of the antibodies to the antigen. The antigen may be a gene product of the HTLV III virus or may be bound to such gene product. On the other hand the antigen may not be a viral gene product but may be produced as a result of the infection and may further be bound to a lymphocyte. The medium may be a human body fluid or a culture medium. A particular embodiment of the present method involves a method for determining the presence of a AIDS virus in a person. The method comprises combining a sample of a body fluid from the person with a monoclonal antibody that binds to an antigen produced as a result of the infection and detecting the binding of the monoclonal antibody to the antigen. The presence of the binding indicates the presence of a AIDS virus infection. Also disclosed are novel monoclonal antibodies, noval compositions of matter, and novel diagnostic kits.

摘要翻译： 公开了一种用于检测培养基中HTLV III感染细胞的存在的方法。 所述方法包括使所述培养基与针对感染结果产生的抗原的单克隆抗体接触并检测抗体与抗原的结合。 抗原可以是HTLV III病毒的基因产物，或者可以与该基因产物结合。 另一方面，抗原可能不是病毒基因产物，但可以作为感染的结果产生，并且可能进一步结合淋巴细胞。 培养基可以是人体液或培养基。 本方法的一个具体实施方案涉及一种用于确定个体中AIDS病毒存在的方法。 该方法包括将来自该人的体液样品与结合与感染结果产生的抗原结合的单克隆抗体并检测单克隆抗体与抗原的结合。 结合物的存在表明艾滋病病毒感染的存在。 还公开了新型单克隆抗体，物质的noval组合物和新型诊断试剂盒。

公开(公告)号：US4917998A

公开(公告)日：1990-04-17

申请号：US860169

申请日：1986-05-06

申请人： Denis R. Burger ,  Andrew S. Goldstein

发明人： Denis R. Burger ,  Andrew S. Goldstein

IPC分类号： C07K14/195 ,  A61K39/395 ,  C07K14/005 ,  C07K14/155 ,  C07K16/00 ,  C07K16/10 ,  C07K19/00 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577

CPC分类号： C07K16/1045 ,  G01N33/56988 ,  Y10S435/81 ,  Y10S435/974 ,  Y10S435/975

摘要： A method is disclosed for detecting the presence of HTLV III infected cells in a medium. The method comprises contacting the medium with monoclonal antibodies against an antigen produced as a result of the infection and detecting the binding of the antibodies to the antigen. The antigen may be a gene product of the HTLV III virus or may be bound to such gene product. On the other hand the antigen may not be a viral gene product by may be produced as a result of the infection and may further be bound to a lymphocyte. The medium may be a human body fluid or a culture medium. A particular embodiment of the present method involves a method for determining the presence of an AIDS virus in a person. The method comprises combining a sample of a body fluid from the person with a monoclonal antibody that binds to an antigen produced as a result of the infection and detecting the binding of the monoclonal antibody to the antigen. The presence of the binding indicates the presence of an AIDS virus infection. Also disclosed are novel monoclonal antibodies, novel compositions of matter, and novel diagnostic kits.

摘要翻译： 公开了一种用于检测培养基中HTLV III感染细胞的存在的方法。 所述方法包括使所述培养基与针对感染结果产生的抗原的单克隆抗体接触并检测抗体与抗原的结合。 抗原可以是HTLV III病毒的基因产物，或者可以与该基因产物结合。 另一方面，作为感染的结果，抗原可能不是病毒基因产物，并且可能进一步结合淋巴细胞。 培养基可以是人体液或培养基。 本方法的一个具体实施方案涉及一种用于确定个体中AIDS病毒存在的方法。 该方法包括将来自该人的体液样品与结合与感染结果产生的抗原结合的单克隆抗体并检测单克隆抗体与抗原的结合。 结合物的存在表明艾滋病病毒感染的存在。 还公开了新型单克隆抗体，新物质组合物和新型诊断试剂盒。

公开(公告)号：US5736322A

公开(公告)日：1998-04-07

申请号：US608431

申请日：1996-02-28

申请人： Andrew S. Goldstein

发明人： Andrew S. Goldstein

IPC分类号： G01N33/50 ,  C12Q1/70 ,  A61K38/47 ,  A61K7/28 ,  A01N59/08

CPC分类号： G01N33/50

摘要： A substitute oral fluid standard for testing, calibration, and standardization of devices and methods for collection, storage, and analysis of oral fluids. The oral fluid standard comprises a mucin and a protease inhibitor.

摘要翻译： 用于口腔液收集，储存和分析的装置和方法的测试，校准和标准化的替代口服液标准。 口服液标准品包括粘蛋白和蛋白酶抑制剂。

公开(公告)号：US5339829A

公开(公告)日：1994-08-23

申请号：US935845

申请日：1992-08-25

申请人： Thomas R. Thieme ,  Andrew S. Goldstein ,  Stephen C. Piacentini ,  Nanette M. Klimkow

发明人： Thomas R. Thieme ,  Andrew S. Goldstein ,  Stephen C. Piacentini ,  Nanette M. Klimkow

IPC分类号： A61B10/00 ,  B01L3/00 ,  G01N1/14 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/68 ,  A61B5/00

CPC分类号： B01L3/502 ,  A61B10/0051 ,  G01N33/5302 ,  G01N33/54366 ,  G01N33/569 ,  G01N33/56988 ,  G01N33/6854 ,  A61B2010/0003 ,  A61B2010/0009 ,  G01N2001/149

摘要： A device for obtaining oral fluid containing substances for testing. In one embodiment, the device includes a syringe having a plunger at the end of which an absorbent pad is attached. A test assembly containing the device is also disclosed.

摘要翻译： 一种用于获得含有测试物质的口腔液的装置。 在一个实施例中，该装置包括具有柱塞的注射器，其末端附接有吸收垫。 还公开了一种包含该装置的测试组件。

公开(公告)号：US5234001A

公开(公告)日：1993-08-10

申请号：US863756

申请日：1992-04-06

申请人： Andrew S. Goldstein ,  David F. Zogg

发明人： Andrew S. Goldstein ,  David F. Zogg

IPC分类号： A61B10/00 ,  B01L3/00 ,  G01N33/53 ,  G01N33/68

CPC分类号： B01L3/502 ,  A61B10/0051 ,  G01N33/5302 ,  G01N33/6854 ,  A61B2010/0003 ,  A61B2010/0009

摘要： A container for storing collected substances for subsequent testing having an open upper end adapted to be sealed with a removable stopper and a lower end having an opening communicating the interior of the container with the outside. The opening is selectively sealed by a frangible nipple during storage of the substances and unsealed for removal of the collected substances for subsequent testing. The distal end of the frangible nipple is enlarged, preferably in the form of a ball.

摘要翻译： 用于储存用于随后测试的收集物质的容器，其具有适于用可移除塞子密封的敞开的上端，下端具有将容器的内部与外部连通的开口。 在储存物质期间，开口由易碎的乳头选择性地密封，并且用于去除所收集的物质用于随后的测试。 易碎乳头的远端被放大，优选地以球的形式。

公开(公告)号：US08611992B2

公开(公告)日：2013-12-17

申请号：US13541641

申请日：2012-07-03

申请人： Andrew S. Goldstein ,  Frank Bellizzi

发明人： Andrew S. Goldstein ,  Frank Bellizzi

IPC分类号： A61B5/00

CPC分类号： A61B5/4875 ,  A61B5/4277 ,  A61B5/4848 ,  A61B5/682 ,  A61B10/0051 ,  A61B2562/0209 ,  A61B2562/029 ,  G01N33/48707

摘要： Timed sensing of collection of saliva in a liquid collection element of predetermined volumetric capacity may be used to determine salivary secretion rate, as may be indicative of state of euhydration or dehydration. Sensing of salivary flow rate may be further augmented by sensing concentration of at least one analyte in saliva (e.g., with an immunochromatographic assay performed in a lateral flow device) in order to determine a state of euhydration or dehydration. Production of saliva may be stimulated, and collected saliva may be analyzed to generate an analyte detection signal that indicative of presence and/or correlative of concentration of at least one analyte in the collected saliva to sense a state of euhydration or dehydration.

摘要翻译： 在预定体积容量的液体收集元件中收集唾液的定时感测可以用于确定唾液分泌速率，这可以指示脱水或脱水的状态。 通过感测唾液中至少一种分析物的浓度（例如，通过在侧流装置中进行的免疫色谱测定）来进一步增强唾液流速的感测，以确定脱水或脱水的状态。 可以刺激唾液的产生，并且可以分析收集的唾液以产生指示所收集的唾液中至少一种分析物的浓度的存在和/或相关性的分析物检测信号，以感测脱水或脱水的状态。

公开(公告)号：US07544468B2

公开(公告)日：2009-06-09

申请号：US09983735

申请日：2001-10-25

申请人： Andrew S. Goldstein ,  Richard K. Bestwick

发明人： Andrew S. Goldstein ,  Richard K. Bestwick

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12Q1/6883 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10S436/811 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/2525 ,  Y10T436/25375 ,  C12Q2565/625

摘要： This invention provides for a rapid and convenient method of simultaneous collection of both genomic and diagnostic information from a single sample on a bibulous pad by differential extraction of the diagnostic information from the genomic information. It is a surprising discovery of this invention that a PCR assay on the contents of the bibulous pad provides results comparable in reliability, specificity, and sensitivity to the best available serum (blood) based assays. The assays of this invention can be used to confirm each other, either by detecting the genomic information leading to the diagnostic information, or by detecting in the genomic information, a predisposition to a disease and confirming the presence of the disease through diagnostic testing.

公开(公告)号：US07192555B2

公开(公告)日：2007-03-20

申请号：US09973956

申请日：2001-10-11

申请人： Ronald W. Mink ,  Andrew S. Goldstein ,  Robert C. Bohannon

发明人： Ronald W. Mink ,  Andrew S. Goldstein ,  Robert C. Bohannon

IPC分类号： G01N33/558

CPC分类号： A61B10/0051 ,  A61B2010/0006 ,  G01N30/90 ,  Y10S435/974 ,  Y10S436/81 ,  Y10T436/25

摘要： A device for collecting and transporting aqueous fluid from the oral cavity to a lateral chromatographic strip for test is disclosed. The lateral chromatographic strip is placed within and extend along a cavity defined in a housing. At least one inspection site to the lateral chromatographic strip is provided to enable inspection of selected sites on the lateral chromatographic strip for test results. A porous wick material protrudes from the housing to a collection site exterior of the housing at one end and communicates to the lateral chromatographic strip at the other end. The porous wick material has particulate construction, the particles adsorbing aqueous oral fluid to transport the fluid from the mouth to the lateral chromatographic strip without substantial absorption. The particles of the porous wick material are bound together to define a continuous interstitial volume for the flow of oral fluid to be transported and are treated to be hydrophilic to the adsorbed oral fluids. The porous wick material readily releases oral fluid to the lateral chromatographic strip. Prevention of reverse flow to the oral cavity from the lateral chromatographic strip naturally occurs due to the circuitous flow path of the porous wick material. A bite plate is coupled to the housing and insertable between the teeth of the patient to position the porous wick in the oral cavity for collecting the oral fluid. The bite plate is typically held in place by the occlusal force of the teeth, preferably the molars and/or the bicuspids, to position the porous wick in the buccal space. By observing the lateral chromatographic strip while the test device is in the mouth immediate test results are obtained.

公开(公告)号：US5073482A

公开(公告)日：1991-12-17

申请号：US209373

申请日：1988-06-21

申请人： Andrew S. Goldstein

发明人： Andrew S. Goldstein

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N15/08

CPC分类号： G01N15/08 ,  C12Q1/6888 ,  C12Q1/70

摘要： A device for testing an elastic membrane for permeability ot submicroscopic particles. The device has a first chamber to contain the particles in a fluid medium; a second chamber to contain a reagent for detecting the presence of any of the particles; suitable structure for mounting the membrane between the chambers in a fluid-tight manner so that the membrane separates the chambers; and a mechanism for applying stress to the membrane.

摘要翻译： 用于测试弹性膜用于亚显微粒子的渗透性的装置。 该装置具有第一室以将颗粒容纳在流体介质中; 第二室，容纳用于检测任何颗粒的存在的试剂; 用于以流体密封的方式将膜安装在室之间的适当结构，使得膜分离室; 以及向膜施加应力的机构。

公开(公告)号：US08326412B2

公开(公告)日：2012-12-04

申请号：US13249521

申请日：2011-09-30

申请人： Andrew S. Goldstein ,  Frank Bellizzi

发明人： Andrew S. Goldstein ,  Frank Bellizzi

IPC分类号： A61B5/05

CPC分类号： A61B5/4875 ,  A61B5/4277 ,  A61B5/4848 ,  A61B5/682 ,  A61B10/0051 ,  A61B2562/0209 ,  A61B2562/029 ,  G01N33/48707

摘要： Timed sensing of collection of saliva in a liquid collection element of predetermined volumetric capacity may be used to determine salivary secretion rate, as may be indicative of state of euhydration or dehydration. Sensing of salivary flow rate may be further augmented by sensing concentration of at least one analyte in saliva (e.g., with an immunochromatographic assay performed in a lateral flow device) in order to determine a state of euhydration or dehydration. Production of saliva may be stimulated, and collected saliva may be analyzed to generate an analyte detection signal that indicative of presence and/or correlative of concentration of at least one analyte in the collected saliva to sense a state of euhydration or dehydration.

摘要翻译： 在预定体积容量的液体收集元件中收集唾液的定时感测可以用于确定唾液分泌速率，这可以指示脱水或脱水的状态。 通过感测唾液中至少一种分析物的浓度（例如，通过在侧流装置中进行的免疫色谱测定）来进一步增强唾液流速的感测，以便确定脱水或脱水的状态。 可以刺激唾液的产生，并且可以分析收集的唾液以产生指示所收集的唾液中至少一种分析物的浓度的存在和/或相关性的分析物检测信号，以感测脱水或脱水的状态。