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    Nucleic acid sequence identification
    1.
    发明申请
    Nucleic acid sequence identification 审中-公开
    核酸序列鉴定

    公开(公告)号：US20060246460A1

    公开(公告)日：2006-11-02

    申请号：US10569698

    申请日：2004-08-26

    申请人： Duncan Graham ,  William Smith ,  Ljiljana Fruk

    发明人： Duncan Graham ,  William Smith ,  Ljiljana Fruk

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： G01N21/658 ,  G01J3/44 ,  G01N2021/653

    摘要： The invention provides modified molecular beacons detectable by surface enhanced Raman spectroscopy (SERS) and related materials, processes, and methods of use. Examples methods provide for the determination of the presence or absence of a target nucleotide sequence in a sample nucleic acid by (a) providing a detection agent, which agent comprises: (i) a probe comprising a target complement sequence (TCS) being complementary to the target sequence and flanking the TCS, first and second oligonucleotide arms, said first and second oligonucleotide arms forming a stem duplex, and said first arm incorporating a first label moiety being detectable by SERS (e.g. a fluoroscein dye) and said second arm terminating in a second label moiety being detectable by SERS, which second arm further includes a surface seeking group (SSG—e.g. an azo-benzotriazole) capable of promoting association of the second label onto an enhancing surface ii) associated with said probe via said SSG, an enhancing surface, such that said first and second label moieties are in close proximity to each other and to the enhancing surface (b) exposing the sample to the detection agent, (c) detecting hybridisation of the TCS to any target sequence present in the sample by a change in the SERS spectra of said agent.

    摘要翻译： 本发明提供通过表面增强拉曼光谱（SERS）和相关材料，方法和使用方法可检测的修饰分子信标。 实例方法通过（a）提供检测剂来确定样品核酸中靶核苷酸序列的存在或不存在，所述检测剂包括：（i）包含与SEQ ID NO：1的互补序列的靶互补序列（TCS）的探针 所述靶序列和所述TCS第一和第二寡核苷酸臂的侧翼，所述第一和第二寡核苷酸臂形成茎双相，并且所述第一臂结合第一标记部分可被SERS（例如氟代染料染料）检测，所述第二臂终止于 第二标记部分可由SERS检测，该第二臂还包括能够促进第二标记与经由所述SSG与所述探针相关联的增强表面ii）的表面寻找组（SSG，例如偶氮 - 苯并三唑）， 使得所述第一和第二标签部分彼此靠近并且与所述增强表面（b）将样品暴露于检测剂 ，（c）通过所述试剂的SERS光谱的变化来检测TCS与样品中存在的任何靶序列的杂交。

    Reclining Chair Assembly
    5.
    发明申请
    Reclining Chair Assembly 有权

    公开(公告)号：US20220257018A1

    公开(公告)日：2022-08-18

    申请号：US17178305

    申请日：2021-02-18

    申请人： William Smith

    发明人： William Smith

    IPC分类号： A47C1/024 ,  A47C7/50

    摘要： A reclining chair assembly includes a reclining chair that has a backrest portion and a chair portion for having a user sit therein. A pair of footrests is each disposed on the chair portion of the reclining chair and each of the footrests is positionable in a lowered position or a raised position to raise the respective user's leg. Moreover, the pair of footrests is independently positionable in the raised position to facilitate one of the user's legs to be lifted while the other of the user's legs is not lifted. A pair of lifting units is each in mechanical communication with a respective one of the pair of footrests. Each of the lifting units independently urges the respective footrest into the raised position or the lowered position.

    Transient Protection Filter Circuit that Minimizes the Effects of Thermal Runaway
    8.
    发明申请
    Transient Protection Filter Circuit that Minimizes the Effects of Thermal Runaway 审中-公开
    瞬态保护滤波电路，尽可能减少热失控的影响

    公开(公告)号：US20140362485A1

    公开(公告)日：2014-12-11

    申请号：US13911305

    申请日：2013-06-06

    申请人： William Smith

    发明人： William Smith

    IPC分类号： H02H9/04

    CPC分类号： H02H9/04 ,  H02H9/001 ,  H02H9/041 ,  H02H9/042

    摘要： Commonly, a transient protection filter circuit is integrated between power sources and the circuits that follow to suppress voltage transients. Pre-existing transient protection filters are implemented such that when a transient is detected, transistors in the path become linear regulators. Instead, this invention uses bridge resistors to form a voltage divider to dissipate the extra power. The novelty of this invention is that it protects the load not by dissipating the extra power in front of the load as other transient protection filter circuits do, but by providing energy from the transient to the load through a resistor. This implementation solves the problem of thermal runaway that is commonly caused when transient voltages are filtered by dissipating power within the transistors in the path of the transient and the electric circuits after the transistors.

    摘要翻译： 通常，瞬态保护滤波器电路集成在电源和随后的电路之间以抑制电压瞬变。 实现了现有的瞬态保护滤波器，使得当检测到瞬态时，路径中的晶体管变为线性稳压器。 相反，本发明使用桥式电阻器来形成分压器以耗散额外功率。 本发明的新颖之处在于，其它瞬态保护滤波器电路不是通过消耗负载前面的额外功率来保护负载，而是通过电阻将瞬态能量提供给负载。 该实现解决了当瞬态电压通过在晶体管之后的瞬态路径和电路中的晶体管内消耗功率而被滤波时通常引起的热失控的问题。