公开(公告)号：US20180275043A1

公开(公告)日：2018-09-27

申请号：US15958480

申请日：2018-04-20

Applicant: EMD Millipore Corporation

Inventor： Philip Janmin Lee ,  Paul Ju-Sung Hung ,  Narendra Maheshri

IPC: G01N15/14 ,  B01L3/00 ,  G01N33/487 ,  G01N33/543 ,  G01N15/00

CPC classification number: G01N15/1484 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0867 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/147 ,  G01N15/1475 ,  G01N33/487 ,  G01N33/54366 ,  G01N2015/0065 ,  G01N2015/1493

Abstract: The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.

公开(公告)号：US10174278B2

公开(公告)日：2019-01-08

申请号：US15175449

申请日：2016-06-07

Applicant: EMD Millipore Corporation

Inventor： Paul Ju-Sung Hung ,  Philip Janmin Lee

IPC: C12M1/32 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  F16K99/00 ,  B29C65/00 ,  G01N33/543 ,  F16K27/02 ,  C12Q1/02 ,  G01N33/50 ,  G01N35/00

Abstract: A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

公开(公告)号：US10054536B2

公开(公告)日：2018-08-21

申请号：US15163398

申请日：2016-05-24

Applicant: EMD Millipore Corporation

Inventor： Philip Janmin Lee ,  Paul Ju-Sung Hung ,  Narendra Maheshri

IPC: G01N15/14 ,  B01L3/00 ,  G01N33/487 ,  G01N33/543 ,  G01N15/00

CPC classification number: G01N15/1484 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0867 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/147 ,  G01N15/1475 ,  G01N33/487 ,  G01N33/54366 ,  G01N2015/0065 ,  G01N2015/1493

Abstract: The device and method relate to microfluidic particle analysis. The device is designed for trapping particles for solution analysis. Exemplary particles include beads and cells. The device has a microfluidics body, and a microfluidics channel formed in the body for receiving particles at an upstream region thereof. The channel has a deformable wall portion that defines a particle-capture region, and is responsive to a change in fluid pressure applied thereto, to selectively vary the particle-flow dimensions of the capture region. In this way, particles having a given size may be selectively retained in the capture region. In one embodiment, the deformable wall portion is expandable in response to a positive fluid pressure applied within the channel. In another embodiment, the deformable wall portion is expandable in response to a negative pressure applied to a cavity communicating with the wall portion, external to the channel.

公开(公告)号：US20160333297A1

公开(公告)日：2016-11-17

申请号：US15175449

申请日：2016-06-07

Applicant: EMD Millipore Corporation

Inventor： Paul Ju-Sung Hung ,  Philip Janmin Lee

IPC: C12M1/32 ,  C12M3/06 ,  G01N33/50 ,  B01L3/00 ,  C12Q1/02 ,  C12M1/00 ,  C12M1/36

CPC classification number: C12M23/12 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/10 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B29C65/002 ,  C12M23/16 ,  C12M29/10 ,  C12M29/14 ,  C12M41/00 ,  C12M41/48 ,  C12Q1/025 ,  F16K27/0236 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  G01N33/5008 ,  G01N33/54366 ,  G01N2035/00158 ,  Y10T137/2202

Abstract: A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

Abstract translation: 公开了一种带阀的微流体装置，微流体细胞培养装置和结合装置的系统。 带阀的微流体装置包括基底，微通道，通过该微通道液体可以在装置内从一个站移动到另一个站;以及气动微型阀，其适于在打开和关闭状态之间切换以控制通过微通道的流体流动。 微型阀由三个柔性膜形成，其中一个柔性膜响应于施加到阀上的气压，另外两个变形以产生更可密封的通道横截面。 细胞培养装置提供阀门以允许将细胞控制加载到装置的个体井中，以及在孔中交换细胞培养组分。

公开(公告)号：US20160327470A1

公开(公告)日：2016-11-10

申请号：US15163398

申请日：2016-05-24

Applicant: EMD Millipore Corporation

Inventor： Philip Janmin Lee ,  Paul Ju-Sung Hung ,  Narendra Maheshri

IPC: G01N15/14 ,  G01N33/543 ,  B01L3/00 ,  G01N33/487

CPC classification number: G01N15/1484 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0867 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/147 ,  G01N15/1475 ,  G01N33/487 ,  G01N33/54366 ,  G01N2015/0065 ,  G01N2015/1493

Abstract: The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.

Abstract translation: 该方法，装置和系统涉及颗粒分析，特别涉及设计用于捕获颗粒进行分析的微流体装置。 颗粒包括珠粒和细胞。

公开(公告)号：US10900886B2

公开(公告)日：2021-01-26

申请号：US15958480

申请日：2018-04-20

Applicant: EMD Millipore Corporation

Inventor： Philip Janmin Lee ,  Paul Ju-Sung Hung ,  Narendra Maheshri

IPC: G01N15/14 ,  G01N33/487 ,  B01L3/00 ,  G01N33/543 ,  G01N15/00

Abstract: The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.

公开(公告)号：US09371929B2

公开(公告)日：2016-06-21

申请号：US14053688

申请日：2013-10-15

Applicant: EMD Millipore Corporation

Inventor： Paul Ju-Sung Hung ,  Philip Janmin Lee

IPC: C12M1/34 ,  F16K27/02 ,  C12M1/32 ,  C12M3/06 ,  C12M1/00 ,  C12M1/36 ,  F16K99/00 ,  B29C65/00 ,  G01N33/543 ,  B01L3/00 ,  G01N35/00

CPC classification number: C12M23/12 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/10 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B29C65/002 ,  C12M23/16 ,  C12M29/10 ,  C12M29/14 ,  C12M41/00 ,  C12M41/48 ,  C12Q1/025 ,  F16K27/0236 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  G01N33/5008 ,  G01N33/54366 ,  G01N2035/00158 ,  Y10T137/2202

Abstract: A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

Abstract translation: 公开了一种带阀的微流体装置，微流体细胞培养装置和结合装置的系统。 带阀的微流体装置包括基底，微通道，通过该微通道液体可以在装置内从一个站移动到另一个站;以及气动微型阀，其适于在打开和关闭状态之间切换以控制通过微通道的流体流动。 微型阀由三个柔性膜形成，其中一个柔性膜响应于施加到阀上的气压，另外两个变形以产生更可密封的通道横截面。 细胞培养装置提供阀门以允许将细胞控制加载到装置的个体井中，以及在孔中交换细胞培养组分。

公开(公告)号：US20140090735A1

公开(公告)日：2014-04-03

申请号：US14053688

申请日：2013-10-15

Applicant: EMD Millipore Corporation

Inventor： Paul Ju-Sung Hung ,  Philip Janmin Lee

IPC: F16K27/02

CPC classification number: C12M23/12 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/10 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B29C65/002 ,  C12M23/16 ,  C12M29/10 ,  C12M29/14 ,  C12M41/00 ,  C12M41/48 ,  C12Q1/025 ,  F16K27/0236 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  G01N33/5008 ,  G01N33/54366 ,  G01N2035/00158 ,  Y10T137/2202

Abstract: A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

Abstract translation: 公开了一种带阀的微流体装置，微流体细胞培养装置和结合装置的系统。 带阀的微流体装置包括基底，微通道，通过该微通道液体可以在装置内从一个站移动到另一个站;以及气动微型阀，其适于在打开和关闭状态之间切换以控制通过微通道的流体流动。 微型阀由三个柔性膜形成，其中一个柔性膜响应于施加到阀上的气压，另外两个变形以产生更可密封的通道横截面。 细胞培养装置提供阀门以允许将细胞控制加载到装置的个体井中，以及在孔中交换细胞培养组分。