公开(公告)号：US20070254378A1

公开(公告)日：2007-11-01

申请号：US11682655

申请日：2007-03-06

Applicant: Hongmin Zhang ,  Egisto Boschetti ,  Lee Lomas ,  Pil-Je Um

Inventor： Hongmin Zhang ,  Egisto Boschetti ,  Lee Lomas ,  Pil-Je Um

IPC: G01N24/00 ,  B01D3/00 ,  B01D59/44 ,  C08F30/04

CPC classification number: C08F220/36 ,  B01D71/28 ,  C02F1/683 ,  C02F2101/20 ,  C08F220/30 ,  C08F222/1006 ,  Y10T436/24

Abstract: This invention provides chelating moieties that comprise an aryl group. Monomers that include the chelating moieties can be polymerized into chelating polymers. Chelating polymers are useful to chelate metals. Chelating polymers in the form of metal chelates are useful for binding analytes, such as polypeptides that comprise histidine residues. Chelating polymers can be includes in articles such as chips and chromatographic materials.

Abstract translation: 本发明提供了包含芳基的螯合部分。 包含螯合部分的单体可以聚合成螯合聚合物。 螯合聚合物可用于螯合金属。 金属螯合物形式的螯合聚合物可用于结合分析物，例如包含组氨酸残基的多肽。 螯合聚合物可以包括在诸如芯片和色谱材料的制品中。

公开(公告)号：US20060216751A1

公开(公告)日：2006-09-28

申请号：US11388181

申请日：2006-03-22

Applicant: Egisto Boschetti ,  Lee Lomas

Inventor： Egisto Boschetti ,  Lee Lomas

IPC: C40B40/04 ,  C40B40/08 ,  C40B40/10

CPC classification number: C07K1/047 ,  C07K1/22

Abstract: The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.

Abstract translation: 本发明提供了用于纯化靶蛋白质组的方法和试剂盒。 该方法包括以下步骤：将包含至少95％的目标蛋白质组和至多5％的污染蛋白质的样品与具有不同结合部分的结合部分的文库结合，结合污染蛋白质和少数靶蛋白质组 到结合部分的文库，将未结合的靶蛋白基团与结合到结合部分文库的蛋白质分离并收集未结合的靶蛋白质。 收集的目标蛋白质比样品中的目标蛋白质组更为纯净。

公开(公告)号：US20090247421A1

公开(公告)日：2009-10-01

申请号：US12162125

申请日：2006-03-22

Applicant: Egisto Boschetti ,  Lee Lomas

Inventor： Egisto Boschetti ,  Lee Lomas

IPC: C40B30/04 ,  C40B50/14 ,  C40B40/10 ,  C40B40/04 ,  C40B40/14

CPC classification number: C07K1/047 ,  G01N33/54326 ,  G01N33/6848

Abstract: The present invention provides diverse chemical libraries bound to small particle with paramagnetic properties. Typically, the chemical structures comprise a plurality of different chemical moieties, the particles are paramagnetic and have a diameter between about 100 nm and about 10 microns, the chemical structures bound to each particular particle have substantially the same structure and the combinatorial library comprises at least 100,000 different chemical structures.

Abstract translation: 本发明提供了与具有顺磁性质的小颗粒结合的不同化学文库。 通常，化学结构包括多个不同的化学部分，颗粒是顺磁性的并且具有约100nm至约10微米之间的直径，与每个特定颗粒结合的化学结构具有基本上相同的结构，组合文库至少包含 100,000种不同的化学结构。

公开(公告)号：US20070059776A1

公开(公告)日：2007-03-15

申请号：US11499898

申请日：2006-08-03

Applicant: Egisto Boschetti ,  Lisa Bradbury ,  Huw Davies ,  Lee Lomas ,  Thang Pham ,  Vanitha Thulasiraman ,  Tai-Tung Yip

Inventor： Egisto Boschetti ,  Lisa Bradbury ,  Huw Davies ,  Lee Lomas ,  Thang Pham ,  Vanitha Thulasiraman ,  Tai-Tung Yip

IPC: G01N33/567 ,  G01N33/53

CPC classification number: G01N33/6842 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  G01N33/6803 ,  G01N2030/8411 ,  H01J49/00 ,  H01J49/0418 ,  G01N30/7233

Abstract: The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.

Abstract translation: 本发明提供了通过产生从多个细胞培养物批次或从给定批次的不同时间采集的样品获取的样品的表面增强的激光解吸/电离质谱图来监测目标多肽的产生的方法。 本发明另外提供了通过产生在纯化过程中的不同时间采集的样品的表面增强的激光解吸/电离质谱图来监测来自混合物的目标多肽的纯化的方法。 此外，本发明涉及鉴定可用于增加给定纯化方法的规模的条件的方法。

公开(公告)号：US20110111978A1

公开(公告)日：2011-05-12

申请号：US13004281

申请日：2011-01-11

Applicant: Egisto Boschetti ,  Lee Lomas

Inventor： Egisto Boschetti ,  Lee Lomas

IPC: C40B30/04 ,  C40B50/14 ,  B82Y5/00

CPC classification number: C07K1/047 ,  C07K1/22

Abstract: The present invention provides diverse chemical libraries bound to small particle with paramagnetic properties. Typically, the chemical structures comprise a plurality of different chemical moieties, the particles are paramagnetic and have a diameter between about 100 nm and about 10 microns, the chemical structures bound to each particular particle have substantially the same structure and the combinatorial library comprises at least 100,000 different chemical structures.

Abstract translation: 本发明提供了与具有顺磁性质的小颗粒结合的不同化学文库。 通常，化学结构包括多个不同的化学部分，颗粒是顺磁性的并且具有约100nm至约10微米之间的直径，与每个特定颗粒结合的化学结构具有基本上相同的结构，组合文库至少包含 100,000种不同的化学结构。

公开(公告)号：US20060110819A1

公开(公告)日：2006-05-25

申请号：US10528820

申请日：2003-09-29

Applicant: Lee Lomas ,  Egisto Boschetti ,  Tai-Tung Yip

Inventor： Lee Lomas ,  Egisto Boschetti ,  Tai-Tung Yip

IPC: C12M1/34

CPC classification number: G01N33/54366 ,  B01J2219/00283 ,  B01J2219/00317 ,  B01J2219/00423 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00695 ,  B01J2219/007 ,  B01J2219/00725 ,  G01N33/54353

Abstract: The present invention relates to the fields of molecular biology, combinatorial chemistry and biochemistry. Particularly, the present invention describes apparatus and methods for the detection and isolation of binding partners and activity modulators for biomolecules. The apparatus described allows for expression, capture and analysis of one or more biomolecules in a single step.

Abstract translation: 本发明涉及分子生物学，组合化学和生物化学领域。 特别地，本发明描述了用于检测和分离用于生物分子的结合配偶体和活性调节剂的装置和方法。 所描述的装置允许在单个步骤中表达，捕获和分析一种或多种生物分子。

公开(公告)号：US20060257946A1

公开(公告)日：2006-11-16

申请号：US10559687

申请日：2004-06-04

Applicant: Jian Ding ,  Lee Lomas

Inventor： Jian Ding ,  Lee Lomas

IPC: G01N33/542 ,  G01N33/53

CPC classification number: G01N33/6887 ,  G01N33/6893 ,  G01N2333/4724 ,  G01N2333/75 ,  G01N2800/324

Abstract: Certain biomarkers and biomarker combinations are useful in a qualifying ischaemic heart disease status in a patient. A diagnostic methodology employing these biomarkers and combinations can distinguish between ischaemic heart disease and normal, as well as between cases of severe myocardial infarction versus mild myocardial infarction.

Abstract translation: 某些生物标志物和生物标志物组合在患者的合格缺血性心脏病状态中是有用的。 使用这些生物标志物和组合的诊断方法可以区分缺血性心脏病和正常，以及严重心肌梗死与轻度心肌梗死的病例。