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公开(公告)号：US20100047533A1
公开(公告)日：2010-02-25
申请号：US12309547
申请日：2007-07-17
申请人： Eva Almansa , Artur Cavaco-Paulo , Harald Chrobaczek , Anita Eberl , Anton Glieder , Georg Gübitz , Andrea Hasmann , Sonja Heumann , Rule Niederstadt , Kai-Uwe Schöning
发明人： Eva Almansa , Artur Cavaco-Paulo , Harald Chrobaczek , Anita Eberl , Anton Glieder , Georg Gübitz , Andrea Hasmann , Sonja Heumann , Rule Niederstadt , Kai-Uwe Schöning
CPC分类号： D06M16/003 , D06M2101/20 , Y10T428/24802 , Y10T442/60
摘要： A new process for enhancing the hydrophilicity of the surface of a polyolefin or polyolefin copolymer article is characterized in that the surface is treated with an enzyme selected from oxidoreductases, especially of the cytochrome P450 family or enzymes classified as EC 1.13 or EC 1.14. The process is especially useful for the treatment of polypropylene films, fibres, or fabrics, inter alia for use in sanitary articles, threads, yarns, fabrics, textiles, garments, technical or household articles, printed or dyed cover films or packaging films.
摘要翻译： 用于增强聚烯烃或聚烯烃共聚物制品的表面的亲水性的新方法的特征在于，用选自氧化还原酶，特别是细胞色素P450家族的酶或分类为EC 1.13或EC 1.14的酶处理表面。 该方法尤其用于处理聚丙烯薄膜，纤维或织物，尤其用于卫生用品，丝线，纱线，织物，织物，服装，技术或家庭用品，印刷或染色的覆盖薄膜或包装膜中。
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2.发明申请R-HNL RANDOM VARIANTS AND THEIR USE FOR PREPARING OPTICALLY PURE, STERICALLY HINDERED CYANOHYDRINS 有权R-HNL随机变量及其用于制备光学纯的，异味的氰基氰公开(公告)号：US20100143986A1
公开(公告)日：2010-06-10
申请号：US12519095
申请日：2007-12-11
申请人： Richard Gaisberger , Anton Glieder , Zhibin Liu , Beate Pscheidt
发明人： Richard Gaisberger , Anton Glieder , Zhibin Liu , Beate Pscheidt
CPC分类号： C12N9/88 , C12P13/004 , C12P41/001
摘要： The invention relates to R-hydroxynitrile lyases with improved substrate acceptance, increased activity and increased selectivity, obtainable by introducing random mutations with the aid of random mutagenesis and/or saturation mutagenesis techniques, identifying by means of screening or selection and, where appropriate, subsequently combining advantageous mutations, and to the use thereof.
摘要翻译： 本发明涉及具有改进的底物接受性，增加的活性和增加的选择性的R-羟基腈裂解酶，其可通过借助于随机诱变和/或饱和诱变技术引入随机突变而获得，通过筛选或选择鉴定，并且在适当的情况下随后 结合有利的突变及其用途。
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公开(公告)号：US20080153126A1
公开(公告)日：2008-06-26
申请号：US11817005
申请日：2006-02-23
申请人： Franz Hartner , Anton Glieder
发明人： Franz Hartner , Anton Glieder
CPC分类号： C12N15/815 , C12Y101/03013
摘要： A mutant Pichia pastoris alcohol oxidase 1 (AOX1) promoter of the wild type Pichia pastoris AOX1 promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of: a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (−784 to −719), nucleotides 170 to 191 (−784 to −763), nucleotides 192 to 213 (−762 to −741), nucleotides 192 to 210 (−762 to −744), nucleotides 207 to 209 (−747 to −745), nucleotides 214 to 235 (−740 to −719), nucleotides 304 to 350 (−650 to −604), nucleotides 364 to 393 (−590 to −561), nucleotides 434 to 508 (−520 to −446), nucleotides 509 to 551 (−445 to −403), nucleotides 552 to 560 (−402 to −394), nucleotides 585 to 617 (−369 to −337), nucleotides 621 to 660 (−333 to −294), nucleotides 625 to 683 (−329 to −271), nucleotides 736 to 741 (−218 to −213), nucleotides 737 to 738 (−217 to −216), nucleotides 726 to 755 (−228 to −199), nucleotides 784 to 800 (−170 to −154) or nucleotides 823 to 861 (−131 to −93) of Seq ID No. 1, and combinations thereof.
摘要翻译： 包含选自以下的至少一个突变的野生型巴斯德毕赤酵母AOX1启动子（SEQ ID No.1）的突变型巴斯德毕赤酵母醇氧化酶1（AOX1）启动子：a）转录因子结合位点（TFBS），b ）核苷酸170至235（-784至-719），核苷酸170至191（-784至-763），核苷酸192至213（-762至-741），核苷酸192至210（-762至-744），核苷酸 207至209（-747至-745），核苷酸214至235（-740至-719），核苷酸304至350（-650至-604），核苷酸364至393（-590至-561），核苷酸434至 508（-520至-446），核苷酸509至551（-445至-403），核苷酸552至560（-402至-394），核苷酸585至617（-369至-337），核苷酸621至660（ -333至-294），核苷酸625至683（-329至-271），核苷酸736至741（-218至-213），核苷酸737至738（-217至-216），核苷酸726至755（-228 至-199），Seq ID No.1的核苷酸784至800（-170至-154）或核苷酸823至861（-131至-93） 及其组合。
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公开(公告)号：US08367386B2
公开(公告)日：2013-02-05
申请号：US13323398
申请日：2011-12-12
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
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公开(公告)号：US20120184015A1
公开(公告)日：2012-07-19
申请号：US13323398
申请日：2011-12-12
IPC分类号： C12N9/02
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译： 提供了编码细胞色素P450变体的核酸。 细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。 优选的野生型细胞色素P450是细胞色素P450 BM-3。 优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。 优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。
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公开(公告)号：US20100196913A1
公开(公告)日：2010-08-05
申请号：US12759763
申请日：2010-04-14
申请人： Franz Hartner , Anton Glieder
发明人： Franz Hartner , Anton Glieder
CPC分类号： C12N15/815 , C12Y101/03013
摘要： A mutant Pichia pastoris alcohol oxidase 1 (AOX1) promoter of the wild type Pichia pastoris AOX1 promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of: a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (−784 to −719), nucleotides 170 to 191 (−784 to −763), nucleotides 192 to 213 (−762 to −741), nucleotides 192 to 210 (−762 to −744), nucleotides 207 to 209 (−747 to −745), nucleotides 214 to 235 (−740 to −719), nucleotides 304 to 350 (−650 to −604), nucleotides 364 to 393 (−590 to −561), nucleotides 434 to 508 (−520 to −446), nucleotides 509 to 551 (−445 to −403), nucleotides 552 to 560 (−402 to −394), nucleotides 585 to 617 (−369 to −337), nucleotides 621 to 660 (−333 to −294), nucleotides 625 to 683 (−329 to −271), nucleotides 736 to 741 (−218 to −213), nucleotides 737 to 738 (−217 to −216), nucleotides 726 to 755 (−228 to −199), nucleotides 784 to 800 (−170 to −154) or nucleotides 823 to 861 (−131 to −93) of Seq ID No. 1, and combinations thereof.
摘要翻译： 一种突变巴斯德毕赤酵母醇氧化酶1（AOX1）野生型毕赤酵母AOX1启动子的启动子（序列号1），其包括选自由以下组成的组中选择的至少一个突变：a）转录因子结合位点（TFBS），B ）核苷酸170到235（-784到-719），核苷酸170至191（-784到-763），核苷酸192至213（-762到-741），核苷酸192至210（-762到-744），核苷酸 207至209（-747到-745），核苷酸214至235（-740到-719），核苷酸304至350（-650到-604），核苷酸364至393（-590到-561），核苷酸434到 508（-520到-446），核苷酸509至551（-445到-403），核苷酸552至560（-402到-394），核苷酸585至617（-369到-337），核苷酸621至660（ -333到-294），核苷酸625至683（-329到-271），核苷酸736至741（-218到-213），核苷酸737至738（-217到-216），核苷酸726至755（-228 至-199），核苷酸784至800（ 170至-154）或核苷酸SEQ ID No.1中，和它们的组合的823至861（-131到-93）。
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公开(公告)号：US07691616B2
公开(公告)日：2010-04-06
申请号：US11800970
申请日：2007-05-07
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译： 提供了编码细胞色素P450变体的核酸。 细胞色素P450变体具有比相应的野生型或亲本细胞色素P450酶更高的烷烃氧化能力，烯烃氧化能力和/或较高的有机溶剂耐受性。 优选的野生型细胞色素P450是细胞色素P450 BM-3。 优选的细胞色素P450变体包括具有改善含有少于8个碳原子的烷烃和环氧化烯烃羟基化能力以及对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些变体。 优选的细胞色素P450变体还包括在包含共溶剂如DMSO和THF的溶液中具有改善的羟基化活性的那些，并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代。
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公开(公告)号：US09012175B2
公开(公告)日：2015-04-21
申请号：US11817005
申请日：2006-02-23
申请人： Franz Hartner , Anton Glieder
发明人： Franz Hartner , Anton Glieder
CPC分类号： C12N15/815 , C12Y101/03013
摘要： A mutant Pichia pastoris alcohol oxidase 1 (AOX1) promoter of the wild type Pichia pastoris AOX1 promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of: a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (−784 to −719), nucleotides 170 to 191 (−784 to −763), nucleotides 192 to 213 (−762 to −741), nucleotides 192 to 210 (−762 to −744), nucleotides 207 to 209 (−747 to −745), nucleotides 214 to 235 (−740 to −719), nucleotides 304 to 350 (−650 to −604), nucleotides 364 to 393 (−590 to −561), nucleotides 434 to 508 (−520 to −446), nucleotides 509 to 551 (−445 to −403), nucleotides 552 to 560 (−402 to −394), nucleotides 585 to 617 (−369 to −337), nucleotides 621 to 660 (−333 to −294), nucleotides 625 to 683 (−329 to −271), nucleotides 736 to 741 (−218 to −213), nucleotides 737 to 738 (−217 to −216), nucleotides 726 to 755 (−228 to −199), nucleotides 784 to 800 (−170 to −154) or nucleotides 823 to 861 (−131 to −93) of Seq ID No. 1, and combinations thereof.
摘要翻译： 包含选自以下的至少一个突变的野生型巴斯德毕赤酵母AOX1启动子（SEQ ID No.1）的突变型巴斯德毕赤酵母醇氧化酶1（AOX1）启动子：a）转录因子结合位点（TFBS），b ）核苷酸170到235（-784到-719），核苷酸170至191（-784到-763），核苷酸192至213（-762到-741），核苷酸192至210（-762到-744），核苷酸 207至209（-747到-745），核苷酸214至235（-740到-719），核苷酸304至350（-650到-604），核苷酸364至393（-590到-561），核苷酸434到 508（-520至-446），核苷酸509至551（-445至-403），核苷酸552至560（-402至-394），核苷酸585至617（-369至-337），核苷酸621至660（ -333至-294），核苷酸625至683（-329至-271），核苷酸736至741（-218至-213），核苷酸737至738（-217至-216），核苷酸726至755（-228 到-199），核苷酸784至800（ 170至-154）或核苷酸SEQ ID No.1中，和它们的组合的823至861（-131到-93）。
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9.发明授权Genes containing a DNA sequence coding for hydroxynitrile lyase, recombinant proteins derived therefrom and having hydroxynitrile lyase activity, and use thereof 有权含有编码羟基腈裂解酶的DNA序列的基因，由其衍生并具有羟基腈裂解酶活性的重组蛋白质及其用途公开(公告)号：US06861243B2
公开(公告)日：2005-03-01
申请号：US10046232
申请日：2002-01-16
申请人： Helmut Schwab , Anton Glieder , Christoph Kratky , Ingrid Dreveny , Peter Pöchlauer , Wolfgang Skranc , Herbert Mayrhofer , Irma Wirth , Rudolf Neuhofer , Rodolfo Bona
发明人： Helmut Schwab , Anton Glieder , Christoph Kratky , Ingrid Dreveny , Peter Pöchlauer , Wolfgang Skranc , Herbert Mayrhofer , Irma Wirth , Rudolf Neuhofer , Rodolfo Bona
CPC分类号： C12P13/004 , C12N9/88 , C12N15/52
摘要： New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5′-region of the mdl genes from Prunus serotina and from Prunus amygdalus and/or a primer 2 based on the 3′-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes from Prunus serotina or from Prunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.
摘要翻译： 含有编码羟基腈裂解酶的DNA序列的新基因，该基因可以通过引物组合基于来自黑麦白浆和来自杏仁核的md1基因的5'区域的DNA序列和/或基于 其中一个羟基腈裂解酶同工酶的一个来自山梨果皮或杏仁杏仁的DNA序列的3'区域，随后用含有编码羟基腈裂解酶的基因的来自生物体的DNA的DNA聚合酶扩增作为模板和克隆，以及 可以通过异源表达所述HNL基因的DNA序列以及从其衍生的蛋白质和融合蛋白质以及所述蛋白质用于制备（R） - 或（S） - 氰基氢化物的方法，在合适的宿主细胞中制备重组蛋白质。
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公开(公告)号：US06790644B2
公开(公告)日：2004-09-14
申请号：US10141225
申请日：2002-05-07
申请人： Grit Straganz , Anton Glieder , Lothar Brecker
发明人： Grit Straganz , Anton Glieder , Lothar Brecker
IPC分类号： C12N902
CPC分类号： C12N9/0069
摘要： An enzyme that cleaves 1,3-dicarbonyl compounds by incorporating molecular oxygen into the substrate in amounts equimolar to the amount of substrate cleaved. The enzyme from Acinetobacter johnsonii is a multimer of about 67 kilodaltons made up of subunits of about 16.6 kilodaltons. Nucleotide and amino acid sequence analysis of a cloned A. johnsonii subunit indicates little homology with other proteins. Native or recombinant enzyme can be used to decontaminate or detoxify acetylacetone or related diketones.
摘要翻译： 通过将分子氧引入底物中的量与切割的底物的量相等的量来切割1,3-二羰基化合物的酶。 来自不动杆菌属的不动杆菌属的酶是由约16.6千道尔顿的亚基组成的约67千道尔顿的多聚体。 克隆的约翰逊亚单位的核苷酸和氨基酸序列分析表明与其他蛋白质几乎没有同源性。 天然或重组酶可用于消除乙酰丙酮或相关二酮的去污或解毒。
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