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    Analyte detection method and analyte detection integrated circuit
    1.
    发明授权
    Analyte detection method and analyte detection integrated circuit 有权

    公开(公告)号：US10041940B2

    公开(公告)日：2018-08-07

    申请号：US13570864

    申请日：2012-08-09

    申请人： Filip Frederix ,  Friso Jacobus Jedema ,  David Van Steenwinckel ,  Hilco Suy

    发明人： Filip Frederix ,  Friso Jacobus Jedema ,  David Van Steenwinckel ,  Hilco Suy

    IPC分类号： G01N33/543 ,  C12Q1/6825 ,  G01N27/414

    摘要： A method for providing an integrated circuit such that first and second sensing electrodes respectively have at their surfaces first and second receptor molecules for selectively binding to first and second analytes of interest; exposing the integrated circuit to a sample potentially comprising at least one of the first and second analytes, providing a first bead having a first electrical signature attached to a first molecule having a conformation/affinity for binding to the first sensing electrode dependent on the presence of the first analyte; providing a second bead having a second electrical signature attached to a second molecule having a conformation/affinity for binding to the second sensing electrode dependent on the presence of the second analyte; and determining the presence of the electrical signature of the first and/or second bead(s) on the first and second sensing electrodes respectively. An IC for implementing this method.

    Molecular sensor using temporal discrimination
    2.
    发明申请
    Molecular sensor using temporal discrimination 审中-公开
    分子传感器使用时间歧视

    公开(公告)号：US20120238473A1

    公开(公告)日：2012-09-20

    申请号：US13420133

    申请日：2012-03-14

    申请人： Evelyne Gridelet ,  Hilco Suy ,  Filip Frederix

    发明人： Evelyne Gridelet ,  Hilco Suy ,  Filip Frederix

    IPC分类号： C40B40/00 ,  B82Y15/00

    CPC分类号： G01N33/557 ,  G01N27/127 ,  G01N33/5438

    摘要： A sensor device is disclosed, which depends on discrimination in time between groups of binding events of target particles to nano-electrodes. The target particles may be in the liquid phase or in suspension. The nano-electrodes form part of a sensor arrangement having a plurality of sensors. The sensor device is arranged such that different species of target particles arrive at the nano-electrodes at different times, using techniques such as chromatography or application of a field such as an electric, magnetic, or gravitational field. The particles may be labelled or unlabeled. The invention is particularly suited, but not limited, to sensing bioparticles.

    摘要翻译： 公开了一种传感器装置，其取决于目标颗粒与纳米电极的结合事件组之间的时间差异。 目标颗粒可以是液相或悬浮液。 纳米电极形成具有多个传感器的传感器装置的一部分。 传感器装置布置成使得不同种类的目标颗粒在不同时间到达纳米电极，使用诸如色谱法或诸如电，磁场或重力场的场的应用的技术。 颗粒可以被标记或未标记。 本发明特别适用于但不限于感测生物颗粒。

    Thiol or disulfide molecules having poly(ethylene oxide) groups for use in a self assembled monolayer bound to a metal layer for covalently immobilizing biomolecules in a biosensor
    5.
    发明授权
    Thiol or disulfide molecules having poly(ethylene oxide) groups for use in a self assembled monolayer bound to a metal layer for covalently immobilizing biomolecules in a biosensor 有权
    具有聚（环氧乙烷）基团的硫醇或二硫化物分子用于与金属层结合的自组装单层，用于在生物传感器中共价固定生物分子

    公开(公告)号：US07770437B2

    公开(公告)日：2010-08-10

    申请号：US11637390

    申请日：2006-12-12

    申请人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

    发明人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

    IPC分类号： G01N37/00

    CPC分类号： G01N33/54353 ,  B05D1/185 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G65/329 ,  C08G65/334 ,  G01N33/54373 ,  G01N2610/00 ,  Y10T29/49002 ,  Y10T428/24

    摘要： An article is provided for immobilizing functional organic biomolecules (e.g. proteins, DNA, and the like) through a covalent bond to a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional biomolecules or bioreceptors could be avoided. The article comprises mixed self-assembled monolayers of thiol or disulfide molecules of formula X1—(CH2)c—O—([CH2]t—CH2—O)n—R1—S—X2 incorporating poly(ethylene oxide) groups and two functional groups, X1 and X2. Preferably, one functional group resists nonspecific adsorption and the other functional group directly (without activation) reacts with functional groups on the biomolecules. The functional group X1 is selected from the group consisting of flurophenyl, fluorobenzoyl, fluorophenoxycarbonyl, nitrophenoxycarbonyl, C2-12 alkenyl, sulfonyl halide, isothiocyanato, isocyanato, carbonyl halide, haloalkylcarbonyl, and diazonium carbonyl. The poly(ethylene oxide) groups resist nonspecific adsorption and enhance the specific affinity interactions. A particularly preferred molecule is 2-(2-{2-[2-(2-{2-[2-(11-mercaptoyldisulfanyl-undecyloxy)ethoxy]ethoxy}ethoxy)ethoxy]ethoxy} acetic acid pentafluorophenyl ester. A sensor device including these monolayers is also provided to perform reproducible, sensitive, specific and stable bioanalysis.

    摘要翻译： 提供了一种用于将功能性有机生物分子（例如蛋白质，DNA等）通过共价键固定到硫醇盐或二硫化物单层到金属表面的物品，其中表面层的额外活化步骤或功能性生物分子的活化步骤 或生物受体可以避免。 该制品包括式X1-（CH2）c-O - （[CH2] t-CH2-O）n-R1-S-X2的结合聚（环氧乙烷）基团的二硫或二硫化物分子的混合自组装单层和两 官能团，X1和X2。 优选地，一个官能团抵抗非特异性吸附，另一个官能团直接（无活化）与生物分子上的官能团反应。 官能团X1选自氟苯基，氟苯甲酰基，氟苯氧基羰基，硝基苯氧基羰基，C2-12烯基，磺酰卤，异硫氰酸酯，异氰酸酯基，羰基卤，卤代烷基羰基和重氮基羰基。 聚（环氧乙烷）基团抵抗非特异性吸附并增强特异性亲和力相互作用。 特别优选的分子是2-（2- {2- [2-（2- {2- [2-（11-巯基二硫烷基 - 十一烷氧基）乙氧基]乙氧基}乙氧基）乙氧基]乙氧基}乙酸五氟苯酯。 还提供了这些单层以进行可重复，敏感，特异和稳定的生物分析。

    Molecules suitable for binding to a metal layer for covalently immobilizing biomolecules
    6.
    发明申请
    Molecules suitable for binding to a metal layer for covalently immobilizing biomolecules 有权
    分子适合与金属层结合，共价固定生物分子

    公开(公告)号：US20070272003A1

    公开(公告)日：2007-11-29

    申请号：US11637390

    申请日：2006-12-12

    申请人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

    发明人： Filip Frederix ,  Kristien Bonroy ,  Karolien Jans

    IPC分类号： G01N37/00 ,  B05D1/00 ,  B32B7/00 ,  C07C291/00 ,  C07C381/00 ,  C07C61/00 ,  C07C61/40 ,  C07D303/02

    CPC分类号： G01N33/54353 ,  B05D1/185 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G65/329 ,  C08G65/334 ,  G01N33/54373 ,  G01N2610/00 ,  Y10T29/49002 ,  Y10T428/24

    摘要： An article is provided for immobilizing functional organic biomolecules (e.g. proteins, DNA, and the like) through a covalent bond to a thiolate or disulfide monolayer to a metal surface wherein an extra activation step of the surface layer or an activation step of the functional biomolecules or bioreceptors could be avoided. The monolayer can contain, but is not limited to, two moieties. One has a group that resists nonspecific adsorption and another has a group that directly (without activation) reacts with functional groups on the biomolecules. In addition, poly(ethylene oxide) groups are incorporated in the monolayer surfaces to resist the nonspecific adsorption and to enhance the specific affinity interactions. A sensor device including these monolayers is also provided to perform reproducible, sensitive, specific and stable bioanalysis.

    摘要翻译： 提供了一种用于将功能性有机生物分子（例如蛋白质，DNA等）通过共价键固定到硫醇盐或二硫化物单层到金属表面的物品，其中表面层的额外活化步骤或功能性生物分子的活化步骤 或生物受体可以避免。 单层可以包含但不限于两个部分。 一个具有抵抗非特异性吸附的基团，另一个具有直接（不活化）与生物分子上的官能团反应的基团。 此外，聚（环氧乙烷）基团并入单层表面以抵抗非特异性吸附并增强特异性亲和力相互作用。 还提供了包括这些单层的传感器装置，以执行可重复，灵敏，特异和稳定的生物分析。

    Method For Detecting An Analyte
    8.
    发明申请
    Method For Detecting An Analyte 审中-公开
    检测分析物的方法

    公开(公告)号：US20080131869A1

    公开(公告)日：2008-06-05

    申请号：US11769237

    申请日：2007-06-27

    申请人： Filip Frederix ,  Gustaaf Borghs ,  Jean-Michel Friedt

    发明人： Filip Frederix ,  Gustaaf Borghs ,  Jean-Michel Friedt

    IPC分类号： C12Q1/70 ,  G01N21/62 ,  G01N33/53 ,  C12Q1/68

    CPC分类号： G01N21/554 ,  B82Y30/00 ,  G01N21/77 ,  G01N33/54373 ,  G01N2021/258 ,  G01N2021/7783

    摘要： The present invention discloses an improved method for detecting an analyte. The present invention may be used for sensing devices which have a higher sensitivity and which can be used to detect very low concentration of analyte. In one embodiment, the method comprises the steps of providing a substrate, said substrate comprising a conductive region and a recognition layer, said conductive region having at least a first surface and a second surface, wherein said first surface is operatively associated with said recognition layer; subjecting said substrate to said analyte such that an interaction occurs between said analyte and said recognition layer; directing radiation through said substrate such that said radiation incidents on said conductive region and said recognition layer; and measuring the intensity of said radiation absorbed or transmitted by said substrate as a function of the wavelength in order to determine the presence of an analyte.

    摘要翻译： 本发明公开了一种用于检测分析物的改进方法。 本发明可以用于具有较高灵敏度并可用于检测非常低浓度分析物的感测装置。 在一个实施例中，该方法包括以下步骤：提供衬底，所述衬底包括导电区域和识别层，所述导电区域具有至少第一表面和第二表面，其中所述第一表面与所述识别层可操作地相关联 ; 使所述衬底经受所述分析物，使得所述分析物与所述识别层之间发生相互作用; 引导辐射穿过所述衬底，使得所述辐射突发在所述导电区域和所述识别层上; 并测量由所述基底吸收或透射的所述辐射的强度作为波长的函数，以便确定分析物的存在。

    DEVICE HAVING SELF-ASSEMBLED-MONOLAYER
    9.
    发明申请
    DEVICE HAVING SELF-ASSEMBLED-MONOLAYER 审中-公开
    具有自组装单元的设备

    公开(公告)号：US20120088315A1

    公开(公告)日：2012-04-12

    申请号：US13378302

    申请日：2010-06-17

    申请人： Thomas Merelle ,  Magali Huguette Alice Lambert ,  Filip Frederix

    发明人： Thomas Merelle ,  Magali Huguette Alice Lambert ,  Filip Frederix

    IPC分类号： G01N33/566 ,  B05D5/12 ,  G01N30/00

    CPC分类号： G01N33/553 ,  G01N33/5438 ,  G01N2610/00

    摘要： A device for bio-sensing applications is disclosed, comprising a substrate such as a semiconductor chip having Cu electrodes thereon, and a self assembled monolayer bonded to at least one of the Cu electrodes, wherein molecules of the self-assembled monolayer comprise a head group which bonds to Cu, a carbon-comprising chain comprising a chain of at least 12 C atoms, and a terminal group which is hydrophilic and for binding a bio-receptor. The terminal group is hydrophilic to allow binding to the bio-receptor, and inclusion of the carbon-comprising chain, limits or avoids corrosion of the copper. Also disclosed is a method of providing such a device, activating the terminal group and coupling a bio-receptor to the activated terminal group. Disclosure further extends to use of such a device for bio-sensing applications.

    摘要翻译： 公开了一种用于生物感测应用的装置，其包括诸如其上具有Cu电极的半导体芯片的衬底和与至少一个Cu电极结合的自组装单层，其中自组装单层的分子包括头组 其与Cu键合，包含至少12个C原子的链的含碳链，以及亲水性并用于结合生物受体的端基。 端基是亲水的，以允许与生物受体结合，并且包含含碳链，限制或避免铜的腐蚀。 还公开了提供这种装置的方法，激活终端组并将生物受​​体与活化的末端基团偶联。 公开进一步延伸到使用这种用于生物感测应用的装置。

    Method and apparatus for detecting an analyte
    10.
    发明授权
    Method and apparatus for detecting an analyte 有权
    用于检测分析物的方法和装置

    公开(公告)号：US08304256B2

    公开(公告)日：2012-11-06

    申请号：US13280133

    申请日：2011-10-24

    申请人： Filip Frederix ,  Kristien Bonroy

    发明人： Filip Frederix ,  Kristien Bonroy

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54346 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N21/554 ,  G01N21/648 ,  G01N2021/258

    摘要： In one aspect, this disclosure provides a substrate for determining the concentration of an analyte within a sample. The substrate includes a conductive region and a recognition layer, the conductive region including at least one particle and having a first surface operatively coupled with the recognition layer, the recognition layer comprising at least one recognition molecule. The distance between the first surface of the conductive region and the recognition molecule is selected such that when the analyte is bound to the recognition layer the combination of the at least one particle and the analyte exhibits at least one of the following effects when radiation is directed through the conductive region and the recognition layer: (i) a particle plasmon effect, (ii) a particle bulk interband absorption, (iii) analyte molecular absorption, and (iv) absorption by the analyte-particle combination.

    摘要翻译： 在一方面，本公开提供了用于确定样品中分析物浓度的底物。 衬底包括导电区域和识别层，该导电区域包括至少一个颗粒并且具有与识别层可操作地耦合的第一表面，识别层包括至少一个识别分子。 选择导电区域的第一表面和识别分子之间的距离，使得当分析物结合到识别层时，当辐射被定向时，至少一个颗粒和分析物的组合显示以下效应中的至少一个 通过导电区域和识别层：（i）颗粒等离子体效应，（ii）颗粒体间带吸收，（iii）分析物分子吸收和（iv）被分析物 - 颗粒组合的吸收。