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    Flow immunosensor method and apparatus
    1.
    发明授权
    Flow immunosensor method and apparatus 失效
    流动免疫传感器方法和装置

    公开(公告)号：US5183740A

    公开(公告)日：1993-02-02

    申请号：US486024

    申请日：1990-02-23

    申请人： Frances S. Ligler ,  Gaber Bruce P. ,  Anne W. Kusterbeck ,  Gregory A. Wemhoff

    发明人： Frances S. Ligler ,  Gaber Bruce P. ,  Anne W. Kusterbeck ,  Gregory A. Wemhoff

    IPC分类号： G01N33/536 ,  G01N33/53 ,  G01N33/543 ,  G01N35/02

    CPC分类号： G01N33/54366 ,  G01N33/53 ,  G01N33/54306 ,  Y10S435/80 ,  Y10S435/804 ,  Y10S435/961 ,  Y10S435/968

    摘要： A method of detecting a target moiety comprising the steps of (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label.Also, the invention is accomplished by a flow immunosensor comprising: a liquid flowing through the immunosensor for moving a sample through the immunosensor; a receiving means for introducing the sample to the liquid stream; a flow control means for moving the liquid stream through the immunosensor; an exchanger connected to the sample receiving means in which the sample is brought in contact with labelled antigens of the target and whereby any target present is allowed to displace the labelled antigen; a detection apparatus connected to the exchanger for detecting any labelled antigen which is released; and a disposal means connected to the detection apparatus for disposing of or collecting of waste coming from the detection apparatus.

    摘要翻译： 一种检测靶部分的方法，其包括以下步骤：（a）提供对靶特异性的抗体，（b）用靶标的标记形式使抗体的结合位点饱和，（c）使包含靶标的液体流过 饱和抗体，从而（d）使靶标置换标记的抗原，（e）用标记的检测器检测置换的标记的抗原。 此外，本发明通过流动免疫传感器实现，该流动免疫传感器包括：流过免疫传感器的液体，用于使样品移动通过免疫传感器; 用于将样品引入液体流的接收装置; 用于使液体流移动通过免疫传感器的流量控制装置; 连接到样品接收装置的交换器，其中样品与靶标的标记抗原接触，并且由此允许存在的任何靶标置换标记抗原; 连接到交换器的检测装置，用于检测被释放的任何标记的抗原; 以及与检测装置连接的处置装置，用于处置或收集来自检测装置的废物。

    Flow immunosensor apparatus
    3.
    发明授权
    Flow immunosensor apparatus 失效
    流量免疫传感器

    公开(公告)号：US06245296B1

    公开(公告)日：2001-06-12

    申请号：US07860965

    申请日：1992-03-31

    申请人： Frances S. Ligler ,  Bruce P. Gaber ,  Anne W. Kusterbeck ,  Gregory A. Wemhoff

    发明人： Frances S. Ligler ,  Bruce P. Gaber ,  Anne W. Kusterbeck ,  Gregory A. Wemhoff

    IPC分类号： G01N3348

    CPC分类号： G01N33/54366 ,  G01N33/53 ,  G01N33/54306 ,  Y10S435/80 ,  Y10S435/804 ,  Y10S435/961 ,  Y10S435/968

    摘要： Target moiety is detected by (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label. This assay is performed by a flow immunosensor comprising: a liquid flowing through the immunosensor for moving a sample through the immunosensor; a sample receiver for introducing the sample to the liquid stream; a flow controller for moving the liquid stream through the immunosensor; an exchanger connected to the sample receiving area in which the sample is brought in contact with labelled antigens of the target and where any target present is allowed to displace the labelled antigen; a detection apparatus connected to the exchanger for detecting any labelled antigen which is released; and a disposal means connected to the detection apparatus for disposing of or collecting of waste coming from the detection apparatus.

    摘要翻译： 通过（a）提供对靶特异性的抗体，（b）用靶标的标记形式使抗体的结合位点饱和，（c）使含有靶标的液体流过饱和抗体，从而（ d）允许目标物置换标记的抗原，和（e）用标记的检测器检测置换的标记的抗原。 该测定由流动免疫传感器进行，包括：流过免疫传感器的液体，用于使样品移动通过免疫传感器; 用于将样品引入液体流的样品接收器; 用于使液体流通过免疫传感器的流量控制器; 连接到样品接收区域的交换器，其中样品与靶标的标记抗原接触，并且允许存在的任何靶标置换标记的抗原; 连接到交换器的检测装置，用于检测被释放的任何标记的抗原; 以及与检测装置连接的处置装置，用于处置或收集来自检测装置的废物。

    Displacement assay on a porous membrane
    5.
    发明授权
    Displacement assay on a porous membrane 失效
    多孔膜上的位移测定

    公开(公告)号：US06750031B1

    公开(公告)日：2004-06-15

    申请号：US08583912

    申请日：1996-01-11

    申请人： Frances S. Ligler ,  Anne W. Kusterbeck ,  Sina Y. Rabbany

    发明人： Frances S. Ligler ,  Anne W. Kusterbeck ,  Sina Y. Rabbany

    IPC分类号： G01N3353

    CPC分类号： G01N33/54386

    摘要： Displacement assays, under non-equilibrium conditions, are performed by flowing a liquid sample through a membrane having binding elements with binding sites saturated with a labelled form of the analyte. Analyte in the sample displaces, under non-equilibrium conditions, the labelled form of the analyte from the membrane. The displaced labelled form of the analyte may then be detected.

    摘要翻译： 在非平衡条件下的位移测定是通过使液体样品流过具有结合位点的膜的方式进行的，所述结合元件具有用标记形式的分析物饱和的结合位点。 样品中的分析物在非平衡条件下移除来自膜的分析物的标记形式。 然后可以检测分析物的置换的标记形式。

    Affinity-based detection of biological targets
    6.
    发明授权
    Affinity-based detection of biological targets 有权
    基于亲和力的生物靶标检测

    公开(公告)号：US08945856B2

    公开(公告)日：2015-02-03

    申请号：US14188784

    申请日：2014-02-25

    申请人： Nadezhda V. Kulagina ,  Chris Rowe Taitt ,  George P. Anderson ,  Frances S. Ligler

    发明人： Nadezhda V. Kulagina ,  Chris Rowe Taitt ,  George P. Anderson ,  Frances S. Ligler

    IPC分类号： G01N33/53 ,  G01N33/569 ,  G01N33/554 ,  G01N33/543

    CPC分类号： G01N33/56916 ,  G01N33/5308 ,  G01N33/54366 ,  G01N33/554 ,  G01N33/56911 ,  G01N33/56983

    摘要： A method of biochemical identification by: providing a plurality of capture species bound to one or more substrates and suspected of having one or more biological targets affinity bound to at least one capture species; detecting which capture species contain bound biological targets to generate a binding pattern; and identifying the biological target based on the binding pattern. The capture species are independently selected from the group consisting of antimicrobial peptides, cytotoxic peptides, antibiotics, and combinations thereof. A device having the capture species bound to the substrates. At least two of the capture species are capable of multi-specific binding to one or more biological targets and may have overlapping but not identical affinity properties.

    摘要翻译： 生物化学鉴定的方法是：提供与一种或多种底物结合的多种捕获物质，并怀疑具有与至少一种捕获物质结合的一种或多种生物靶标; 检测哪些捕获物种含有结合的生物靶标以产生结合模式; 以及基于所述结合模式识别所述生物学目标。 捕获物种独立地选自抗微生物肽，细胞毒性肽，抗生素及其组合。 具有捕获物质与基底结合的装置。 至少两种捕获物质能够与一种或多种生物靶标进行多特异性结合，并且可以具有重叠但不相同的亲和性质。

    Rotationally Actuated Magnetic Bead Trap and Mixer
    7.
    发明申请
    Rotationally Actuated Magnetic Bead Trap and Mixer 有权
    旋转驱动磁珠和混合器

    公开(公告)号：US20110188339A1

    公开(公告)日：2011-08-04

    申请号：US13015731

    申请日：2011-01-28

    申请人： Peter B. Howell, JR. ,  Richard Eitel ,  Joel P. Golden ,  Frances S. Ligler

    发明人： Peter B. Howell, JR. ,  Richard Eitel ,  Joel P. Golden ,  Frances S. Ligler

    IPC分类号： B01F13/08

    CPC分类号： B01F13/0818 ,  B01F7/00908

    摘要： A magnetic bead trap-and-mixer includes a channel having openings at opposing ends, and a rotor adjacent to the channel and comprising a permanent magnet, wherein the rotor is adapted to apply a magnetic field to the channel of sufficient strength to direct the movement of magnetic beads therein. In aspects, the channel is straight and/or has narrowed end. In further aspects, the rotor generates in the channel areas of areas of strong magnetic fields alternating with areas of very weak magnetic fields and the strong magnetic fields extend entirely across the channel.

    摘要翻译： 磁珠捕集器和混合器包括在相对端处具有开口的通道和与通道相邻并包括永磁体的转子，其中转子适于向通道施加足够强度的磁场以引导运动 的磁珠。 在方面，通道是直的和/或具有变窄的端。 在另外的方面，转子在与非常弱的磁场的区域交替的强磁场的区域的通道区域中产生，并且强磁场完全延伸穿过通道。