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    MONOMERS CAPABLE OF DIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME
    5.
    发明申请
    MONOMERS CAPABLE OF DIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME 审中-公开
    可以在水溶液中二维分离的单体及其使用方法

    公开(公告)号：US20140194383A1

    公开(公告)日：2014-07-10

    申请号：US14110056

    申请日：2012-04-09

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    IPC分类号： C07D211/26 ,  C07F5/04

    CPC分类号： C07D211/26 ,  C07D405/06 ,  C07D409/06 ,  C07D491/107 ,  C07F5/025 ,  C07F5/04

    摘要： Described herein are monomers capable of forming a biologically useful multimer when in contact with one, two, three or more other monomers in an aqueous media. In one aspect, such monomers may be capable of binding to another monomer in an aqueous media (e.g. in vivo) to form a multimer, (e.g. a dimer). Contemplated monomers may include a ligand moiety, a linker element, and a connector element that joins the ligand moiety and the linker element. In an aqueous media, such contemplated monomers may join together via each linker element and may thus be capable of modulating one or more biomolecules substantially simultaneously, e.g., modulate two or more binding domains on a protein or on different proteins.

    摘要翻译： 本文描述的是当在水性介质中与一种，两种，三种或更多种其它单体接触时，能够形成生物有用的多聚体的单体。 在一个方面，这样的单体可以能够在水性介质（例如体内）中结合另一单体以形成多聚体（例如二聚体）。 考虑的单体可以包括配体部分，连接体元件和连接配体部分和连接体元件的连接器元件。 在水性介质中，这样预期的单体可以经由每个连接体元件连接在一起，因此可以基本上同时调节一种或多种生物分子，例如调节蛋白质上或不同蛋白质上的两个或多个结合结构域。

    SILYL MONOMERS CAPABLE OF MULTIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME
    6.
    发明申请
    SILYL MONOMERS CAPABLE OF MULTIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME 审中-公开
    可在水溶液中多元化的SILYL单体及其使用方法

    公开(公告)号：US20140163229A1

    公开(公告)日：2014-06-12

    申请号：US14110060

    申请日：2012-04-09

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    IPC分类号： C07F7/10

    CPC分类号： A61K31/695 ,  A61K47/55 ,  C07F7/10

    摘要： Described herein are silyl monomers capable of forming a biologically useful multimer when in contact with one, two, three or more other monomers in an aqueous media. Such multimer forming associations of monomers may be promoted by the proximal binding of the monomers to their target biomolecule(s). In one aspect, such monomers may be capable of binding to another monomer in an aqueous media (e.g. in vivo) to form a multimer, (e.g. a dimer). Contemplated monomers may include a ligand moiety, a linker element, and a connector element that joins the ligand moiety and the linker element. In an aqueous media, such contemplated monomers may join together via each linker element and may thus be capable of modulating one or more biomolecules substantially simultaneously, e.g., modulate two or more binding domains on a protein or on different proteins.

    摘要翻译： 本文描述的是当在水性介质中与一种，两种，三种或更多种其它单体接触时能够形成生物学上有用的多聚体的甲硅烷基单体。 单体的这种多聚体形成关联可以通过单体与其目标生物分子的近端结合来促进。 在一个方面，这样的单体可以能够在水性介质（例如体内）中结合另一单体以形成多聚体（例如二聚体）。 考虑的单体可以包括配体部分，连接体元件和连接配体部分和连接体元件的连接器元件。 在水性介质中，这样预期的单体可以经由每个连接体元件连接在一起，因此可以基本上同时调节一种或多种生物分子，例如调节蛋白质上或不同蛋白质上的两个或多个结合结构域。

    COFLUORONS AND METHODS OF MAKING AND USING THEM
    7.
    发明申请
    COFLUORONS AND METHODS OF MAKING AND USING THEM 审中-公开
    COFLORONS AND METHODS OF MAKING AND USING THEM

    公开(公告)号：US20140161729A1

    公开(公告)日：2014-06-12

    申请号：US14110019

    申请日：2012-04-09

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    IPC分类号： G01N33/573 ,  C07D405/06 ,  C07D491/107 ,  C07D211/26 ,  C07D263/06 ,  C07F5/02 ,  C07D401/06

    CPC分类号： G01N33/573 ,  C07D211/26 ,  C07D263/06 ,  C07D401/06 ,  C07D405/06 ,  C07D491/107 ,  C07F5/025 ,  C40B30/04 ,  C40B30/06 ,  C40B50/10 ,  C40B50/16

    摘要： The present invention is directed to method of using a collection of monomers capable of forming multimers as a fluorescence reporter in different applications such as ligand detection/screening, disease diagnosis, drug discovery or screening, fluorescent labeling and imaging, or other fluorescent methodologies. Each monomer in the collection includes one or more ligand elements useful for binding to a target molecule with a dissociation constant of less than 300 μM and a linker element connected to the ligand elements directly or indirectly through a connector. Association of linker elements of different combinations of monomers, with their ligand elements bound to the target molecule to form a multimer, will generate a unique fluorescent signature different from that produced by those monomers either alone or in association with each other in the absence of the target molecule, when subjected to electromagnetic excitement.

    摘要翻译： 本发明涉及在不同应用如配体检测/筛选，疾病诊断，药物发现或筛选，荧光标记和成像或其它荧光方法中使用能够形成多聚体的单体的集合的方法。 收集物中的每个单体包括一个或多个配体元件，其用于结合具有小于300μM的解离常数的靶分子，以及通过连接器直接或间接连接到配体元件的连接体元件。 不同单体组合的连接体元件与其与靶分子结合以形成多聚体的连接体元件的关联将产生独特的荧光特征，其不同于单独或彼此相关联的单体，不存在 目标分子，当受到电磁兴奋时。

    DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING FLUORESCENCE RESONANCE ENERGY TRANSFER
    8.
    发明申请
    DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING FLUORESCENCE RESONANCE ENERGY TRANSFER 审中-公开
    使用荧光共振能量转移检测目标核酸序列

    公开(公告)号：US20110136116A1

    公开(公告)日：2011-06-09

    申请号：US12934866

    申请日：2009-04-08

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald Bergstrom

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald Bergstrom

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6827 ,  Y10T436/143333 ,  C12Q2561/125 ,  C12Q2527/107 ,  C12Q2525/301

    摘要： A method for identifying a plurality of target nucleic acid molecules in a sample. The method provides a plurality of oligonucleotide probe sets. Each set comprises a first and a second probe, each having a target-specific portion and a tunable portion with an acceptor or a donor group. The first probe further comprises an endcapped hairpin. A reaction comprises a denaturation and hybridization cycle. Under the hybridization, the set of probes hybridize in a base-specific manner to their respective target nucleotide sequences, and ligate to one another to form a ligation product. Under conditions that permit hybridization of the tunable portions of the ligation product to one another, an internally hybridized ligation product formed, which allows the detection of the fluorescence resonance energy transfer (FRET). A method comprising PCR amplification is also disclosed.

    摘要翻译： 用于鉴定样品中多个靶核酸分子的方法。 该方法提供多个寡核苷酸探针组。 每组包括第一和第二探针，每个具有目标特异性部分和具有受体或供体基团的可调部分。 第一探针还包含封端的发夹。 反应包括变性和杂交循环。 在杂交下，探针组以碱基特异性方式与其各自的靶核苷酸序列杂交，并且彼此连接以形成连接产物。 在允许连接产物的可调部分彼此杂交的条件下，形成内部杂交的连接产物，其允许检测荧光共振能量转移（FRET）。 还公开了包括PCR扩增的方法。

    Methods for identifying target nucleic acid molecules
    9.
    发明申请
    Methods for identifying target nucleic acid molecules 审中-公开
    识别靶核酸分子的方法

    公开(公告)号：US20050266417A1

    公开(公告)日：2005-12-01

    申请号：US10939294

    申请日：2004-09-10

    申请人： Francis Barany ,  Daniel Turner ,  Maneesh Pingle ,  Hanna Pincas

    发明人： Francis Barany ,  Daniel Turner ,  Maneesh Pingle ,  Hanna Pincas

    IPC分类号： C12N20060101 ,  C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  Y02A50/55 ,  Y02A50/57 ,  Y02A50/58 ,  C12Q2561/125 ,  C12Q2565/102 ,  C12Q2563/155

    摘要： The present invention relates to methods for identifying target nucleic acid molecules differing by one or more single-base changes, insertions, deletions, or translocations; and identifying one or more target mRNA molecules differing by one or more splice site variations in a plurality of mRNA molecules. Also disclosed is a method of generating a linearly amplified representation of a whole genome. Other aspects of the present invention relate to labeled detection oligonucleotide probes and translational oligonucleotide probes as well as to methods of designing such probes.

    摘要翻译： 本发明涉及用于鉴定不同于一个或多个单碱基变化，插入，缺失或易位的靶核酸分子的方法; 以及鉴定由多个mRNA分子中的一个或多个剪接位点变异而不同的一个或多个靶mRNA分子。 还公开了一种产生全基因组的线性放大表示的方法。 本发明的其它方面涉及标记的检测寡核苷酸探针和翻译寡核苷酸探针，以及设计这种探针的方法。