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公开(公告)号：US06846654B1
公开(公告)日：2005-01-25
申请号：US08090245
申请日：1993-07-09
IPC分类号： C07K16/10 , C07K16/42 , C07K16/44 , C12N9/00 , C12P1/00 , C12P13/22 , C12P17/10 , C12P19/34 , C12P41/00 , C12Q1/25 , G01N33/535 , G01N33/542 , G01N33/543 , G01N33/544 , G01N33/53 , C07K16/00
CPC分类号： B82Y5/00 , C07K16/1063 , C07K16/4241 , C07K16/44 , C12N9/0002 , C12P1/00 , C12P13/227 , C12P17/10 , C12P19/34 , C12P41/004 , C12Q1/25 , G01N33/535 , G01N33/542 , G01N33/5438 , G01N33/544 , G01N2333/16 , G01N2333/96472 , Y10S435/817
摘要： A system for detecting physical or chemical changes in an environment is disclosed. The system comprises means for conducting a chemical reaction; an active biological molecule whose physiological function is noncatalytic which is capable of catalyzing a chemical reaction wherein at least one reactant is converted to one product; and means for sensing and processing information relating to changes in said environment. A method for detecting physical or chemical changes in an environment is also disclosed.
摘要翻译： 公开了一种用于检测环境中物理或化学变化的系统。 该系统包括用于进行化学反应的装置; 活性生物分子，其生理功能是非催化的，其能够催化化学反应，其中至少一种反应物转化为一种产物; 以及用于感测和处理与所述环境的变化有关的信息的装置。 还公开了一种用于检测环境中的物理或化学变化的方法。
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2.发明授权公开(公告)号：US06881589B1
公开(公告)日：2005-04-19
申请号：US08465443
申请日：1995-06-05
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/543 , G01N33/58 , G01N33/53 , G01N21/00 , G01N33/544 , G01N33/566
CPC分类号： C12Q1/6825 , C07H21/00 , C12Q1/6816 , C12Q1/686 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N2458/30 , Y10S436/805 , Y10S436/806 , C12Q2565/628 , C12Q2563/113 , C12Q2563/103
摘要： The invention provides compositions and kits for performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence. The compositions and kits comprise an electrochemiluminescent label, collectable particles, binding reagents, and an electrolyte.
摘要翻译： 本发明提供了基于电化学发光对样品中存在的感兴趣分析物进行结合测定的组合物和试剂盒。 组合物和试剂盒包括电化学发光标记，可收集颗粒，结合试剂和电解质。
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3.发明授权Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets 失效包括多个磁体的基于磁性微粒的发光测定方法和装置
公开(公告)号：US5705402A
公开(公告)日：1998-01-06
申请号：US255824
申请日：1994-06-08
申请人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
CPC分类号： G01N21/69 , C12Q1/6825 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： Disclosed and claimed are methods and apparatus for performing a binding assay for an analyte of interest present in a sample based upon measurement of electrochemiluminescence at an electrode. The method uses magnetically responsive particles. The method and apparatus call for a plurality of electromagnets or permanent magnets in north-south orientation for imposing a magnetic field so as to collect the particles.
摘要翻译： 公开并要求保护的是基于电极上的电化学发光测量，对样品中存在的感兴趣分析物进行结合测定的方法和装置。 该方法使用磁响应颗粒。 所述方法和装置要求用于施加磁场以收集颗粒的南北取向的多个电磁体或永磁体。
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公开(公告)号：US6078782A
公开(公告)日：2000-06-20
申请号：US473313
申请日：1995-06-07
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/58 , G01N33/553
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/686 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemiluminescence is related to the amount of analyte in the sample.
摘要翻译： 本发明涉及基于感兴趣的电极处的电化学发光对样品中存在的感兴趣分析物进行结合测定的方法。 在该方法中使用颗粒，然后将其收集在可以诱导电化学发光的区域中，其中诱导的电化学发光的量与样品中分析物的量相关。
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5.发明授权Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence and chemiluminescence detection 失效用于使用颗粒浓度，化学发光和化学发光检测的电化学产生的改进发光测定的装置
公开(公告)号：US5746974A
公开(公告)日：1998-05-05
申请号：US467028
申请日：1995-06-06
CPC分类号： G01N21/69 , C12Q1/6825 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有所述样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物特异性结合的多个包被的磁性颗粒， /或所述测定性能物质; 孵育所述组合物以形成包含颗粒和所述标记组分的复合物; 在电极表面磁性收集所述复合物; 通过使其与触发器接触来诱导所述标签发光，所述触发器通过在引入电化学能量时转化前体分子而原地形成; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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公开(公告)号：US07462322B2
公开(公告)日：2008-12-09
申请号：US10912574
申请日：2004-08-06
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： G01N21/76 , G01N33/553 , G01N27/26 , C12M1/00
CPC分类号： G01N33/582 , C07H21/00 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438
摘要： An apparatus for performing a binding assay for an analyte of interest present in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising a cell defining a sample containing volume intersecting with inlet and outlet means, an electrode having a substantially horizontally positioned surface exposed to and positioned below a portion of the sample containing volume, means for impressing electrochemical energy upon said electrode sufficient to generate luminescence, means for magnetically collecting particles along said surface and means for measuring the luminescence generated at said electrode.
摘要翻译： 基于在包括限定与入口和出口装置相交的体积的样品的细胞的电极表面上测量电化学发光，对样品中存在的感兴趣分析物进行结合测定的装置，具有暴露于 并且位于所述样品容纳体积的一部分下方，用于在所述电极上施加足以产生发光的电化学能量的装置，用于沿着所述表面磁性收集颗粒的装置和用于测量在所述电极处产生的发光的装置。
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公开(公告)号：US5962218A
公开(公告)日：1999-10-05
申请号：US160063
申请日：1993-11-24
申请人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Gary F. Blackburn , Richard J. Massey
发明人： Jonathan K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Gary F. Blackburn , Richard J. Massey
IPC分类号： C12Q1/68 , G01N1/40 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/553
CPC分类号： G01N21/69 , C12Q1/6825 , G01N21/76 , G01N33/5438 , G01N1/40
摘要： The invention relates to methods, apparatus, reagents, and kits for performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. In the method, reagents and kits particles can be employed; for instance, for settling upon the electrode surface by gravity, centrifugation or magnetic attraction. The apparatus can include a magnet for generating a magnetic field in a region proximate the electrode.
摘要翻译： 本发明涉及用于基于感兴趣的电极处的电化学发光对样品中存在的感兴趣分析物进行结合测定的方法，装置，试剂和试剂盒。 在该方法中，可以使用试剂和试剂盒颗粒; 例如，通过重力，离心或磁吸引来沉积在电极表面上。 该装置可以包括用于在靠近电极的区域中产生磁场的磁体。
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8.发明授权Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection 失效用于使用粒子浓度化学发光检测改进发光测定的方法和装置公开(公告)号：US06448091B1
公开(公告)日：2002-09-10
申请号：US08335183
申请日：1994-11-07
IPC分类号： G01N2176
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6825 , G01N1/40 , G01N21/69 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N33/585 , G01N2458/30 , C12Q2565/102 , C12Q2563/131 , C12Q2563/103
摘要： Methods and apparatus for performing a binding assay for an analyte of interest present in a sample are described. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物和/或 测定性能物质; 孵育组合物以形成包含颗粒和标记组分的复合物; 在收集区收集复合物; 向收集区引入能够触发标签以使标签发光的触发器; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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公开(公告)号：US06325973B1
公开(公告)日：2001-12-04
申请号：US09253558
申请日：1999-02-19
申请人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
发明人： John K. Leland , Haresh P. Shah , John H. Kenten , Jack E. Goodman , George E. Lowke , Yuzaburo Namba , Gary F. Blackburn , Richard J. Massey
IPC分类号： G01N2726
摘要： An apparatus for performing a binding assay for an analyte of interest in a sample based upon measurement of clectrochemiluminescence at an electrode surface comprising: (a) a cell defining a sample; (b) an electrode adjacent a portion of the sample containing volume; (c) a voltage control device for impressing electrochemical energy upon the electrode sufficient to generate luminescence; (d) means for magnetically collecting particles along the electrode surface; and (e) a light detection device for measuring the luminescence.
摘要翻译： 一种用于基于电极表面上的电化学发光测量来对样品中感兴趣的分析物进行结合测定的装置，包括：（a）限定样品的细胞; （b）邻近样品容纳体积的一部分的电极; （c）电压控制装置，用于在足以产生发光的电极上施加电化学能; （d）沿着电极表面磁性收集颗粒的装置; 和（e）用于测量发光的光检测装置。
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10.发明授权Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection 失效用于使用粒子浓度和化学发光检测改善发光测定的装置
公开(公告)号：US5798083A
公开(公告)日：1998-08-25
申请号：US469464
申请日：1995-06-06
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/58
CPC分类号： G01N21/69 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N33/585 , G01N1/40 , G01N2458/30
摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物和/或 测定性能物质; 孵育组合物以形成包含颗粒和标记组分的复合物; 在收集区收集复合物; 向收集区引入能够触发标签以使标签发光的触发器; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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