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1.发明授权Controlled electrochemical deposition of polysaccharide films and hydrogels, and materials formed therefrom 有权多糖膜和水凝胶的控制电化学沉积及由其形成的材料公开(公告)号：US07883615B2
公开(公告)日：2011-02-08
申请号：US10541057
申请日：2004-02-11
申请人： Gregory F. Payne , Li-Qun Wu , Reza Ghodssi , William E. Bentley , Gary W. Rubloff , Hyunmin Yi , Rohan Fernandes , Tianhong Chen , David A. Small
发明人： Gregory F. Payne , Li-Qun Wu , Reza Ghodssi , William E. Bentley , Gary W. Rubloff , Hyunmin Yi , Rohan Fernandes , Tianhong Chen , David A. Small
IPC分类号： C25D9/02
CPC分类号： C25D5/022 , C09D5/4419
摘要： A method is provided for electrochemically depositing a polysaccharide mass having a selected physical state. According to an embodiment, an electrically conductive support of a substrate is contacted with an aqueous solution including a selectively insolubilizable polysaccharide, and the selectively insolubilizable polysaccharide is electrochemically deposited on the electrically conductive support while controlling deposition conditions to form the polysaccharide mass having the selected physical state, such as that of a hydrogel. Deposition may be performed in a spatially and/or temporally controlled manner.
摘要翻译： 提供了一种用于电化学沉积具有选定物理状态的多糖物质的方法。 根据一个实施方案，将基材的导电载体与包含选择性不溶性多糖的水溶液接触，并且将选择性不溶性多糖电化学沉积在导电载体上，同时控制沉积条件以形成具有选定物理性质的多糖物质 状态，例如水凝胶的状态。 沉积可以在空间和/或时间上控制的方式进行。
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公开(公告)号：US07820227B2
公开(公告)日：2010-10-26
申请号：US10581905
申请日：2004-02-20
申请人： Gregory F. Payne , Gary W. Rubloff , Hyunmin Yi , Rohan Fernandes , Li-Qun Wu , Reza Ghodssi , William E. Bentley
发明人： Gregory F. Payne , Gary W. Rubloff , Hyunmin Yi , Rohan Fernandes , Li-Qun Wu , Reza Ghodssi , William E. Bentley
IPC分类号： A61L33/00
CPC分类号： G03F7/40 , A61L31/10 , A61L31/14 , Y10T428/31504 , Y10T428/31536 , Y10T428/31971 , C08L5/08
摘要： A method for biolithographical deposition of molecules is provided. According to an embodiment of the method, a reactive layer (e.g., a polysaccharide mass) having a surface region coated with a biologically compatible resist is provided. A portion of the biologically compatible resist is selectively removed to expose an exposed portion of the reactive layer. Molecules, such as biomolecules and/or cellular species, are then conjugated to the exposed portion of the reactive layer. Also provided are materials and devices related to the method.
摘要翻译： 提供了分子生物层析沉积的方法。 根据该方法的一个实施方案，提供具有涂覆有生物相容性抗蚀剂的表面区域的反应层（例如，多糖质量）。 选择性地除去生物相容性抗蚀剂的一部分以暴露反应层的暴露部分。 分子，例如生物分子和/或细胞物质，然后与反应层的暴露部分共轭。 还提供了与该方法相关的材料和装置。
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3.发明授权Spatially selective deposition of polysaccharide layer onto patterned template 有权多糖层在图案化模板上的空间选择性沉积公开(公告)号：US07790010B2
公开(公告)日：2010-09-07
申请号：US11155116
申请日：2005-06-17
申请人： William E. Bentley , Reza Ghodssi , Gregory F. Payne , Gary W. Rubloff , Li-Qun Wu , Hyunmin Yi , Wolfgang Losert , Douglas S. English
发明人： William E. Bentley , Reza Ghodssi , Gregory F. Payne , Gary W. Rubloff , Li-Qun Wu , Hyunmin Yi , Wolfgang Losert , Douglas S. English
CPC分类号： C09D105/08 , C08B37/003 , C09D5/4419 , C25D9/02
摘要： A method is provided for electrochemically depositing a polymer with spatial selectivity. A substrate having a substrate surface is contacted with an aqueous solution containing a selectively insolubilizable polysaccharide, such as chitosan, which is subjected to electrochemically treatment to deposit, with spatial selectivity, the selectively insolubilizable polysaccharide on a patterned electrically conductive portion of the substrate surface.
摘要翻译： 提供了一种以空间选择性电化学沉积聚合物的方法。 具有基底表面的基底与含有选择性不溶性多糖（例如壳聚糖）的水溶液接触，其经过电化学处理以空间选择性沉积在基底表面的图案化导电部分上的选择性不溶性多糖。
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公开(公告)号：US20110081677A1
公开(公告)日：2011-04-07
申请号：US12890017
申请日：2010-09-24
申请人： Xiaolong Luo , Gary W. Rubloff , Dean L. Berlin , William E. Bentley , Susan Buckhout-White , Yi Cheng , Jordan Betz
发明人： Xiaolong Luo , Gary W. Rubloff , Dean L. Berlin , William E. Bentley , Susan Buckhout-White , Yi Cheng , Jordan Betz
CPC分类号： C08B37/003 , B01L3/502753 , B01L2200/0636 , B01L2200/0647 , B01L2300/0681 , B01L2300/0864 , B01L2300/0867 , C08L5/04 , C08L5/08 , C12M21/18 , C12M23/16 , C12N11/10
摘要： The present invention relates to a biofabricated Active Microfluidic Membrane (AMM) in a microfluidic network of a microfluidic device and a method for the in situ biofabrication of such a microfluidic network. More specifically, the invention relates to devices exhibiting (and methods of) positioning (i.e., erecting, modifying or removing a membrane matrix in situ in a microchannel of a microfluidic network of a microfluidic device. In one embodiment, the membrane comprises a single type of matrix constituent, such as chitosan, alginate, etc. Alternatively, the membrane may be composed of two or more matrix constituents, which may be integrated into one another or layered adjacent to one another.
摘要翻译： 本发明涉及微流控装置的微流体网络中的生物制造的活性微流体膜（AMM）和用于这种微流体网络的原位生物制造的方法。 更具体地说，本发明涉及在微流体装置的微流体网络的微通道中进行定位（即，在原位上架设，修饰或除去膜基质）的装置。在一个实施方案中，膜包括单一类型 的基质成分，例如壳聚糖，藻酸盐等。或者，膜可以由两种或更多种基质组分组成，其可以彼此集成或相互叠合。
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5.发明授权Phosphorylated and branched dihydroxy-pentane-dione (DPD) analogs as quorum sensing inhibitors in bacteria 有权磷酸化和支化的二羟基 - 戊烷二酮（DPD）类似物作为细菌中的群体感应抑制剂公开(公告)号：US08952192B2
公开(公告)日：2015-02-10
申请号：US13430000
申请日：2012-03-26
申请人： Herman O. Sintim , William E. Bentley , Varnika Roy , Jacqueline Smith , Reza Ghodssi , Mariana Tsacoumis Meyer
发明人： Herman O. Sintim , William E. Bentley , Varnika Roy , Jacqueline Smith , Reza Ghodssi , Mariana Tsacoumis Meyer
IPC分类号： C07C69/16 , C07C235/80 , C07C49/337 , C07D307/46 , A61K31/12 , A61K31/121 , A01N35/02 , C07F9/09 , C07C317/24 , C07C49/82 , C07C205/45 , C07C49/17 , A01N43/08 , C07C69/28 , A01N37/12 , A01N35/04 , C07F9/38 , A01N57/12 , C07D241/42 , C07D405/04
CPC分类号： C07C317/24 , A01N35/02 , A01N35/04 , A01N37/12 , A01N43/08 , A01N57/12 , C07C49/17 , C07C49/337 , C07C49/82 , C07C69/16 , C07C69/28 , C07C205/45 , C07C235/80 , C07D241/42 , C07D307/46 , C07D405/04 , C07F9/091 , C07F9/3808
摘要： Provided are compositions and methods for modulating quorum sensing in microbes. The compounds are AI-2 analogs and as such have structures similar to 4,5-dihydroxy-2,3-pentanedione that can act as agonists/antagonists of quorum sensing. The compounds are useful for modulating quorum sensing in bacteria and can be used in methods for prophylaxis or therapy of bacterial infections and for reduction of biofilms.
摘要翻译： 提供了调节微生物群体感应的组合物和方法。 化合物是AI-2类似物，因此具有类似于4,5-二羟基-2,3-戊二酮的结构，其可以作为群体感应的激动剂/拮抗剂。 该化合物可用于调节细菌中的群体感应，并可用于预防或治疗细菌感染和减少生物膜的方法。
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6.发明授权Fibrous assemblies for antibody presentation, and multiplexed antigenic analysis using same 有权用于抗体呈递的纤维组件，以及使用其的多重抗原分析公开(公告)号：US08791239B2
公开(公告)日：2014-07-29
申请号：US12990981
申请日：2009-04-07
CPC分类号： A61K9/5169 , B01J19/0046 , B01J2219/00515 , B01J2219/00524 , B01J2219/00527 , B01J2219/00657 , B01J2219/00659 , B01J2219/00666 , B01J2219/00725 , C08L5/08 , G01N33/544
摘要： Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g., in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis. In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.
摘要翻译： 包括纤维平台和组氨酸标记的蛋白质的生物功能化纤维和任选的抗体。 壳聚糖是用作纤维平台的纤维。 纤维平台可以用镍处理，或者可以直接连接到组氨酸标记的蛋白质，例如组氨酸标记的链球菌IgG结合蛋白，蛋白G，蛋白质G3T，GFP或RFP。 所得的生物功能化纤维可以通过各种生物制造方法组装成蛋白质纤维组件。 纤维组件，例如织造织物的形式，可用于（i）抗原捕获; （ii）免疫分析和/或（iii）多重分析。 在一个制造中，纤维组件的每个纤维呈现特异性抗体，并且纤维的混合和匹配，例如通过编织来自各种抗体呈递纤维的织物，允许同时捕获多个抗原进行多重分析。
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7.发明申请FIBROUS ASSEMBLIES FOR ANTIBODY PRESENTATION, AND MULTIPLEXED ANTIGENIC ANALYSIS USING SAME 有权用于抗体呈现的纤维组装和使用相同的多重抗原分析公开(公告)号：US20110195860A1
公开(公告)日：2011-08-11
申请号：US12990981
申请日：2009-04-07
CPC分类号： A61K9/5169 , B01J19/0046 , B01J2219/00515 , B01J2219/00524 , B01J2219/00527 , B01J2219/00657 , B01J2219/00659 , B01J2219/00666 , B01J2219/00725 , C08L5/08 , G01N33/544
摘要： Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g., in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis. In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.
摘要翻译： 包括纤维平台和组氨酸标记的蛋白质的生物功能化纤维和任选的抗体。 壳聚糖是用作纤维平台的纤维。 纤维平台可以用镍处理，或者可以直接连接到组氨酸标记的蛋白质，例如组氨酸标记的链球菌IgG结合蛋白，蛋白G，蛋白质G3T，GFP或RFP。 所得的生物功能化纤维可以通过各种生物制造方法组装成蛋白质纤维组件。 纤维组件，例如织造织物的形式，可用于（i）抗原捕获; （ii）免疫分析和/或（iii）多重分析。 在一个制造中，纤维组件的每个纤维呈现特异性抗体，并且纤维的混合和匹配，例如通过编织来自各种抗体呈递纤维的织物，允许同时捕获多个抗原进行多重分析。
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8.发明授权公开(公告)号：US06759195B1
公开(公告)日：2004-07-06
申请号：US09534366
申请日：2000-03-24
申请人： William E. Bentley , Ryan Gill
发明人： William E. Bentley , Ryan Gill
IPC分类号： C12Q168
CPC分类号： C12N15/1096
摘要： The present invention provides a method of differential display of prokaryotic messages RNA by RTPCR, the method comprising the steps of: adding a first primer mixture to a first nucleic acid sample including a first mixture of mRNA to form a first primer/first nucleic acid sample mixture; adding the first primer mixture to a second nucleic acid sample including a first mixture of mRNA to form a first primer/second nucleic acid sample mixture; incubating the first primer/first nucleic acid sample mixture to produce a first population of cDNA; incubating the first primer/second nucleic acid sample mixture to produce a second population of cDNA; adding a second primer to the first population of cDNA to form a second primer/first population of cDNA mixture; adding the second primer mixture to the second population of cDNA to form a second primer/second population of cDNA mixture; amplifying the second primer/first population of cDNA mixture to produce a third population of cDNA; amplifying the second primer/second population of cDNA mixture to produce a fourth population of cDNA; identifying the presence or level of mRNA in the third population of cDNA; and identifying the presence or level of mRNA in the fourth population of cDNA.
摘要翻译： 本发明提供了通过RTPCR差异显示原核信息RNA的方法，所述方法包括以下步骤：将第一引物混合物加入到包含mRNA的第一混合物的第一核酸样品中以形成第一引物/第一核酸样品 混合物; 将第一引物混合物加入到包含mRNA的第一混合物的第二核酸样品中以形成第一引物/第二核酸样品混合物; 孵育第一引物/第一核酸样品混合物以产生第一个cDNA群; 孵育第一引物/第二核酸样品混合物以产生第二群cDNA; 向第一群cDNA加入第二引物以形成cDNA混合物的第二引物/第一群; 将第二引物混合物加入到第二群cDNA中以形成cDNA混合物的第二引物/第二群; 扩增cDNA混合物的第二引物/第一群体以产生cDNA的第三群; 扩增cDNA混合物的第二引物/第二群体以产生第四个cDNA群; 鉴定第三组cDNA中mRNA的存在或水平; 并鉴定第四组cDNA中mRNA的存在或水平。
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公开(公告)号：USRE39270E1
公开(公告)日：2006-09-05
申请号：US09952668
申请日：2001-09-14
IPC分类号： C12P21/06 , C12P21/02 , A61K67/033
CPC分类号： C07K14/55 , C07K14/43595
摘要： The present invention provides for a recombinant insect larvae and a process of manufacturing proteins utilizing insect larvae that allows for the selection of individual larvae for harvest at the point of their optimal expression of a protein of interest. This invention also provides for a process to manufacture proteins in larvae that does not require synchronization of the infection, growth and harvest larvae to optimally manufacture a protein of interest. The invention further provides for a process of producing interleukin-2 in larvae.
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公开(公告)号：US6153409A
公开(公告)日：2000-11-28
申请号：US927804
申请日：1997-09-11
IPC分类号： C07K14/435 , C07K14/55 , C12P21/04 , C12P21/06
CPC分类号： C07K14/55 , C07K14/43595
摘要： The present invention provides for a recombinant insect larvae and a process of manufacturing proteins utilizing insect larvae that allows for the selection of individual larvae for harvest at the point of their optimal expression of a protein of interest. This invention also provides for a process to manufacture proteins in larvae that does not require synchronization of the infection, growth and harvest larvae to optimally manufacture a protein of interest. The invention further provides for a process of producing interleukin-2 in larvae.
摘要翻译： 本发明提供重组昆虫幼虫和使用昆虫幼虫制造蛋白质的方法，其允许在目标蛋白质的最佳表达点选择单个幼虫进行收获。 本发明还提供了一种在幼虫中制造蛋白质的方法，其不需要同步感染，生长和收获幼虫以最佳地制造目标蛋白质。 本发明还提供了在幼虫中产生白细胞介素-2的方法。
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