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    CUVETTE-BASED APPARATUS FOR BLOOD COAGULATION MEASUREMENT AND TESTING
    1.
    发明申请
    CUVETTE-BASED APPARATUS FOR BLOOD COAGULATION MEASUREMENT AND TESTING 审中-公开
    基于CUVETTE的设备用于血液凝固测量和测试

    公开(公告)号：US20100028207A1

    公开(公告)日：2010-02-04

    申请号：US12503665

    申请日：2009-07-15

    申请人： Gregory M. Colella ,  Henry D. Huang ,  Anthony F. Kuklo, JR. ,  Dimitri V. Shishkin ,  Maria Figueroa ,  James A. Mawhirt

    发明人： Gregory M. Colella ,  Henry D. Huang ,  Anthony F. Kuklo, JR. ,  Dimitri V. Shishkin ,  Maria Figueroa ,  James A. Mawhirt

    IPC分类号： G01N33/49 ,  B01L3/00

    CPC分类号： G01N33/4905 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/16 ,  B01L2300/027 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/165 ,  B01L2400/0487 ,  B01L2400/086

    摘要： An apparatus for measuring blood clotting time includes a blood clot detection instrument and a cuvette for use with the blood clot detection instrument. The cuvette includes a blood sample receptor-inlet; a channel arrangement including at least one test channel for performing a blood clotting time measurement, a sampling channel having at least one surface portion that is hydrophilic, communicating with the blood sample receptor-inlet and the at least one test channel, and a waste channel having at least one surface portion that is hydrophilic, communicating with the sampling channel; and a vent opening communicating with the sampling channel. The sampling channel, the vent opening and the waste channel, coact to automatically draw a requisite volume of a blood sample deposited at the blood receptor-inlet, into the sampling channel. More specifically, air compressed within the blood clot detection instrument, the at least one test channel of the cuvette, and the section of the sampling channel extending beyond the vent opening of the cuvette, coacts with the waste channel to cause a leading edge of the blood sample drawn into the sampling channel from the blood receptor-inlet, to pull back within the sampling channel and uncover an optical sensor in of the blood clot detection instrument. The uncovering of the optical sensor activates a pump module of the blood clot detection instrument, which draws the requisite volume of the blood sample into the at least one test channel.

    摘要翻译： 用于测量血液凝固时间的装置包括血块检测仪器和与血块检测仪器一起使用的试管。 比色皿包括血液样本受体 - 入口; 包括用于进行血液凝固时间测量的至少一个测试通道的通道布置，具有至少一个亲水性表面部分，与血液样本受体入口和至少一个测试通道连通的采样通道，以及废物通道 具有至少一个亲水的表面部分，与采样通道连通; 以及与采样通道连通的通风口。 采样通道，通气口和废气通道共同作用，将沉积在血液受体入口处的血液样本的必需体积自动绘制到采样通道中。 更具体地，在血块检测仪器内压缩的空气，比色皿的至少一个测试通道以及延伸超过比色杯通气口的采样通道的部分与废物通道共同作用， 血液样本从血液受体入口吸入采样通道，在采样通道内拉回，并露出血块检测仪器中的光学传感器。 光学传感器的露出激活血块检测仪器的泵模块，其将血样的必要体积吸入至少一个测试通道。

    Methods for the identification and characterization of reticulocytes in whole blood
    3.
    发明授权
    Methods for the identification and characterization of reticulocytes in whole blood 失效
    全血中网织红细胞的鉴定和表征方法

    公开(公告)号：US5350695A

    公开(公告)日：1994-09-27

    申请号：US802593

    申请日：1991-12-05

    申请人： Gregory M. Colella ,  Daniel Ben-David ,  Albert Cupo ,  Sophie S. Fan ,  Gena Fischer ,  Grace E. Martin ,  Leonard Ornstein

    发明人： Gregory M. Colella ,  Daniel Ben-David ,  Albert Cupo ,  Sophie S. Fan ,  Gena Fischer ,  Grace E. Martin ,  Leonard Ornstein

    IPC分类号： G01N15/14 ,  G01N1/30 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N33/5094 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

    摘要： Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include organic cationic dyes for staining the reticulocytes in the blood sample and buffer solutions for maintaining a pH of about 6 to about 9. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. One method also includes adjusting the measured absorption signals for pseudo-absorption.

    摘要翻译： 表征和区分网织红细胞和成熟红细胞的方法使用试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。染料可以是蓝色吸收染料恶嗪 750或新亚甲基蓝。 当用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂时，并且将试剂组合物和全血样品混合物通过流式细胞仪的感测区域，测量每个细胞散射和吸收的光， 红细胞可以与网织红细胞区分开，并从测定的细胞 - 细胞计算出网织红细胞或红细胞的体积，血红蛋白浓度和每种网织红细胞或红细胞的血红蛋白含量，平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白， 细胞内体积和血红蛋白浓度。 一种方法还包括调整测量的吸收信号用于伪吸收。

    Methods for the identification and characterization of reticulocytes in whole blood
    7.
    发明授权
    Methods for the identification and characterization of reticulocytes in whole blood 失效
    全血中网织红细胞的鉴定和表征方法

    公开(公告)号：US5360739A

    公开(公告)日：1994-11-01

    申请号：US802585

    申请日：1991-12-05

    申请人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    发明人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N15/1459 ,  G01N33/5094 ,  G01N1/38 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1486 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

    摘要： Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

    摘要翻译： 表征和区分网织红细胞和成熟红细胞的方法使用试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。染料可以是红色可激发 荧光染料Oxazine 750，或蓝色可激发荧光染料吖啶橙或吖啶橙衍生物。 当用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂时，试剂组合物和全血样品混合物通过流式细胞计数器的感测区域时，散射在至少一个角度间隔的光， 测量每个细胞发荧光的红细胞，网织红细胞和网织红细胞或红细胞的体积，血红蛋白浓度和血红蛋白含量以及网织红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白和/ 或红细胞从测量的细胞逐个体积和血红蛋白浓度计算。

    Highly sensitive, accurate, and precise automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples
    8.
    发明授权
    Highly sensitive, accurate, and precise automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples 失效
    用于识别和定量血小板以及使用全血样品测定血小板激活状态的高度灵敏，准确和精确的自动化方法和装置

    公开(公告)号：US6025201A

    公开(公告)日：2000-02-15

    申请号：US884595

    申请日：1997-06-27

    申请人： David Zelmanovic ,  Gregory M. Colella ,  Edward J. Hetherington ,  Evelyn Sabrinah Chapman ,  Lynn Paseltiner

    发明人： David Zelmanovic ,  Gregory M. Colella ,  Edward J. Hetherington ,  Evelyn Sabrinah Chapman ,  Lynn Paseltiner

    IPC分类号： G01N15/14 ,  G01N33/48

    CPC分类号： G01N15/1459 ,  G01N15/12 ,  G01N2015/0084 ,  G01N2015/1037 ,  G01N2015/1402

    摘要： The present invention provides a highly sensitive and accurate method and system for the discrimination and quantification of platelets in a whole blood sample using automated hematology instruments. The method and system of the invention provide the accurate measurements of platelet dry mass and platelet component concentration in both normal blood samples and in abnormal blood samples, such as those from thrombocytopenic patients. The determination of platelet dry mass and platelet component concentration can serve to assess the activation state of platelets since activated platelets possess measurably lower component concentrations and refractive indices than do non-activated platelets. The method and system of the invention also allows the clinician or skilled practitioner to determine the age of a blood sample on the basis of the measured parameter of platelet component concentration.

    摘要翻译： 本发明提供了一种用于使用自动血液仪器鉴别和定量全血样品中血小板的高灵敏度和准确的方法和系统。 本发明的方法和系统提供正常血液样品和异常血液样品（例如来自血小板减少症患者）的血小板干质量和血小板成分浓度的准确测量。 血小板干质量和血小板成分浓度的测定可用于评估血小板的活化状态，因为活性血小板具有比非活化血小板低的成分浓度和折射指数。 本发明的方法和系统还允许临床医师或熟练医师根据血小板成分浓度的测量参数确定血液样本的年龄。

    Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood
    10.
    发明授权
    Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood 失效
    试剂组合物及其在全血中鉴定和表征网织红细胞的用途

    公开(公告)号：US5411891A

    公开(公告)日：1995-05-02

    申请号：US961582

    申请日：1992-10-15

    申请人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    发明人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N15/1459 ,  G01N33/5094 ,  G01N1/38 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1486 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

    摘要： A reagent composition which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9 is provided. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. A zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes. The reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

    摘要翻译： 提供了一种试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。 染料可以是红色可激发荧光染料Oxazine 750，或蓝色可激发荧光染料吖啶橙或吖啶橙衍生物。 用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂。 试剂组合物和全血样品混合物通过流式细胞仪的感测区域，测量散布在至少一个角度间隔内并由每个细胞发荧光的光，红细胞可与网织红细胞和体积，血红蛋白浓度 并根据测定的细胞间细胞体积和血红蛋白浓度计算网织红细胞或红细胞的血红蛋白含量，以及网织红细胞和/或红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白。