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    Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use
    1.
    发明申请
    Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use 审中-公开
    具有封闭的超薄二氧化硅层的磁性颗粒，其生产方法及其用途

    公开(公告)号：US20100063263A1

    公开(公告)日：2010-03-11

    申请号：US11917922

    申请日：2006-06-13

    申请人： Guido Hennig ,  Karlheinz Brand

    发明人： Guido Hennig ,  Karlheinz Brand

    IPC分类号： C07H21/04 ,  H01F1/00 ,  B05D5/12 ,  C07H21/02

    CPC分类号： C12N15/1013 ,  C12Q1/6806 ,  H01F1/01 ,  H01F1/112 ,  H01F1/36 ,  H01F41/005

    摘要： The invention relates to magnetic particles coated with silica (Si02), whereby the silicate layer is closed and tight and is characterized by having an extremely small thickness on the scale of a few nanometers—hereafter also referred to as a silica nanolayer. This invention also relates to an improved method for producing these silicate-containing magnetic particles that, in comparison to the prior art, lead to a product having a closed silicate layer and thus entail a highly improved purity. In addition, the novel method prevents an uncontrolled formation of aggregates and clusters of silicates on the magnetite surface, thereby having a positive influence on the properties and biological applications cited below. The novel method also enables the depletion of nanoparticulate solid substance particles on the basis of a fractionated centrifugation. The inventive magnetic particles exhibit an optimized magnetization and suspension behavior as well as a very advantageous run-off behavior from plastic surfaces. These highly pure magnetic particles coated with silicon dioxide are preferably used for isolating nucleic acids from cell and tissue samples, whereby the separating out from a sample matrix ensues by means of magnetic fields. These particles are particularly well suited for the automatic purification of nucleic acids, mostly from biological body samples for the purpose of analyzing them with different amplification methods.

    摘要翻译： 本发明涉及涂覆有二氧化硅（SiO 2）的磁性颗粒，由此硅酸盐层封闭且紧密，其特征在于具有几纳米尺度的极小厚度（以下也称为二氧化硅纳米层）。 本发明还涉及一种生产这些含硅酸盐的磁性颗粒的改进方法，与现有技术相比，其导致具有封闭的硅酸盐层的产品，从而需要高度改善的纯度。 此外，该新颖的方法防止在磁铁矿表面上不受控制地形成聚集体和硅酸盐簇，从而对以下引用的性质和生物应用产生积极的影响。 该新颖的方法还能够在分级离心的基础上消耗纳米颗粒固体物质颗粒。 本发明的磁性颗粒表现出优化的磁化和悬浮性能以及来自塑料表面的非常有利的流失特性。 涂覆有二氧化硅的这些高度纯的磁性颗粒优选用于从细胞和组织样品中分离核酸，由此通过磁场分离出样品基质。 这些颗粒特别适用于核酸的自动纯化，主要来自生物体样品，以便用不同的扩增方法分析它们。

    Method For Purification Of Nucleic Acids, Particularly From Fixed Tissue
    2.
    发明申请
    Method For Purification Of Nucleic Acids, Particularly From Fixed Tissue 有权
    核酸纯化方法，特别是固定组织的纯化方法

    公开(公告)号：US20110245483A1

    公开(公告)日：2011-10-06

    申请号：US13133182

    申请日：2009-11-20

    申请人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

    发明人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

    IPC分类号： C07H1/06 ,  B65G47/22

    CPC分类号： C12Q1/6806 ,  C07B63/00 ,  C12N15/1013 ,  Y10T137/8376

    摘要： The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

    摘要翻译： 本发明涉及核酸纯化方法，用于实施本发明方法的试剂盒和用于纯化生物样品的磁性颗粒的新应用。 根据本发明的方法包括以下步骤：a）将样品容纳在水溶液中的第一样品容器中并在非离液状态下裂解样品; 在溶液中悬浮第一磁性颗粒并将第一样品容器插入样品容器保持器中，其中样品容器插入与样品容器保持器相关联的环形磁体的环形内部空间中; 将溶液与磁性颗粒分离; 并从溶液中分离核酸。

    Process for producing an intensity distribution over a working laser beam and a device for this purpose
    3.
    发明授权
    Process for producing an intensity distribution over a working laser beam and a device for this purpose 失效
    用于在工作激光束上产生强度分布的工艺和用于此目的的装置

    公开(公告)号：US06462307B1

    公开(公告)日：2002-10-08

    申请号：US09613828

    申请日：2000-07-11

    申请人： Guido Hennig ,  Max Datwyler

    发明人： Guido Hennig ,  Max Datwyler

    IPC分类号： B23K2600

    CPC分类号： B23K26/073

    摘要： In a process for producing a stipulated intensity distribution of a working laser beam on an object surface, at least one first and one second component beam with a stipulated first and second intensity distribution are formed and the power of each component beam is changed in a controlled manner with switching times down to submicroseconds. The component beams at a third intensity distribution superimposed as the working beam are pointed at or into the object in order to achieve a different action as a result of a varied third intensity distribution of the working beam. In contrast to conventional processes the first intensity distribution Ib=a×fb(x, y, t) differs from the following one In=a×fn(x, y, t) by the value of the function fu(x, y, t) and no longer only by an altered linear value a. The process as claimed in the invention can be preferably used in the production of screen cells on an engraved cylinder.

    摘要翻译： 在用于在物体表面上产生工作激光束的规定的强度分布的过程中，形成具有规定的第一和第二强度分布的至少一个第一和第二分量光束，并且每个分量光束的光焦度被控制 方式与切换时间下降到亚微秒。 作为工作光束叠加的第三强度分布的分量光束被指向物体或物体，以便由于工作光束的变化的第三强度分布而实现不同的动作。 与常规处理相比，第一强度分布Ib = axfb（x，y，t）与下面的一个In = axfn（x，y，t）的不同在于函数fu（x，y，t）的值和no 只有通过改变的线性值a才更长。 本发明所述的方法可以优选用于生产雕刻圆柱体上的筛孔。

    Method For Filtering Nucleic Acids, In Particular From Fixed Tissue
    4.
    发明申请
    Method For Filtering Nucleic Acids, In Particular From Fixed Tissue 有权
    用于过滤核酸的方法，特别是来自固定组织的方法

    公开(公告)号：US20110092691A1

    公开(公告)日：2011-04-21

    申请号：US12997903

    申请日：2009-06-18

    申请人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

    发明人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

    IPC分类号： C07H21/00 ,  H01F1/00

    CPC分类号： C12N15/1013

    摘要： The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

    摘要翻译： 本发明涉及一种过滤核酸的方法，用于实施本发明方法的试剂盒以及用于过滤生物样品的磁性颗粒的新用途。 根据本发明的方法包括以下步骤：a）将样品保持在水溶液中; b）裂解样品; c）分离细胞碎片; 和d）从溶液中分离核酸，步骤（a）至（c）在非离液性条件下进行。

    Method for filtering nucleic acids, in particular from fixed tissue
    5.
    发明授权
    Method for filtering nucleic acids, in particular from fixed tissue 有权
    用于过滤核酸，特别是固定组织的方法

    公开(公告)号：US08846897B2

    公开(公告)日：2014-09-30

    申请号：US12997903

    申请日：2009-06-18

    申请人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

    发明人： Heike Euting ,  Guido Hennig ,  Kerstin Bohmann

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12N15/10

    CPC分类号： C12N15/1013

    摘要： The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

    摘要翻译： 本发明涉及一种过滤核酸的方法，用于实施本发明方法的试剂盒以及用于过滤生物样品的磁性颗粒的新用途。 根据本发明的方法包括以下步骤：a）将样品保持在水溶液中; b）裂解样品; c）分离细胞碎片; 和d）从溶液中分离核酸，步骤（a）至（c）在非离液性条件下进行。

    Method for purification of nucleic acids, particularly from fixed tissue
    6.
    发明授权
    Method for purification of nucleic acids, particularly from fixed tissue 有权
    核酸特别是固定组织的纯化方法

    公开(公告)号：US08703931B2

    公开(公告)日：2014-04-22

    申请号：US13133182

    申请日：2009-11-20

    申请人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

    发明人： Heike Euting ,  Guido Hennig ,  Alexandre Izmailov

    IPC分类号： C07H21/00

    CPC分类号： C12Q1/6806 ,  C07B63/00 ,  C12N15/1013 ,  Y10T137/8376

    摘要： The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

    摘要翻译： 本发明涉及核酸纯化方法，用于实施本发明方法的试剂盒和用于纯化生物样品的磁性颗粒的新应用。 根据本发明的方法包括以下步骤：a）将样品容纳在水溶液中的第一样品容器中并在非离液状态下裂解样品; 在溶液中悬浮第一磁性颗粒并将第一样品容器插入样品容器保持器中，其中样品容器插入与样品容器保持器相关联的环形磁体的环形内部空间中; 将溶液与磁性颗粒分离; 并从溶液中分离核酸。

    Method and apparatus for producing wells in the surface of a rotogravure cyclinder with a laser
    10.
    发明授权
    Method and apparatus for producing wells in the surface of a rotogravure cyclinder with a laser 失效
    用于在具有激光的轮转凹版旋转器的表面中生产孔的方法和装置

    公开(公告)号：US5856648A

    公开(公告)日：1999-01-05

    申请号：US765089

    申请日：1996-12-20

    申请人： Jakob Frauchiger ,  Andreas C. Brockelt ,  Guido Hennig

    发明人： Jakob Frauchiger ,  Andreas C. Brockelt ,  Guido Hennig

    IPC分类号： B41C1/05 ,  B23K26/0622 ,  B23K26/073 ,  B23K26/08

    CPC分类号： B41C1/05 ,  B23K26/0622 ,  B23K26/0823

    摘要： A method and a device for particularly rapidly producing wells (18) in the surface of a rotating rotogravure cylinder (2) by a pulsed laser beam (13), which is deflected and directed via an optical lens (7) onto the surface of the rotogravure cylinder (2) to be processed. The power of a single laser beam pulse is adjusted in such a way that it is sufficient for removing the maximum volume needed for a well (18). Furthermore, the laser beam (13) is deflected by an optoacoustic modulator (8) by diffraction onto the optical axis of the lens (7), and the individual laser beam pulses are controlled in their pulse power by the same modulator (8) in accordance with the volumes of the wells to be formed.

    摘要翻译： PCT No.PCT / CH96 / 00130 Sec。 371日期1996年12月20日第 102（e）1996年12月20日PCT PCT 1996年4月12日PCT公布。 第WO96 / 34718号公报 日期1996年11月7日一种用于通过脉冲激光束（13）在旋转凹版凹版滚筒（2）的表面特别快速地生产孔（18）的方法和装置，该脉冲激光束通过光学透镜（7）偏转和定向， 到待处理的凹版凹版滚筒（2）的表面上。 调整单个激光束脉冲的功率使其足以去除井（18）所需的最大体积。 此外，激光束（13）被光声调制器（8）通过衍射偏转到透镜（7）的光轴上，并且各个激光束脉冲通过相同的调制器（8）在其脉冲功率中被控制 根据要形成的井的体积。