公开(公告)号：US20180265908A1

公开(公告)日：2018-09-20

申请号：US15760348

申请日：2016-06-22

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Tadashi FUKAMI ,  Hidenao YAMADA

IPC: C12Q1/04 ,  G01N21/45 ,  G02B21/36

CPC classification number: C12Q1/04 ,  C12M41/38 ,  G01N15/1434 ,  G01N15/1468 ,  G01N21/41 ,  G01N21/45 ,  G01N33/5073 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2201/063 ,  G01N2400/00 ,  G02B21/00 ,  G02B21/361 ,  G02B21/365

Abstract: The present invention relates to a cell determination method for determining a cell type based on a content of glycogen in a cell, including: an acquisition step of acquiring optical path length data by measuring an optical path length of the cell; a calculation step of calculating an optical path length indicator correlated with the optical path length of the cell from the obtained optical path length data; a comparison step of comparing the calculated optical path length indicator with a threshold; and a determination step of determining whether the cell is a cell type having a high glycogen content in the cell or a cell type having a low glycogen content in the cell, based on the comparison result.

公开(公告)号：US20160334384A1

公开(公告)日：2016-11-17

申请号：US15218369

申请日：2016-07-25

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Norikazu SUGIYAMA ,  Tadashi FUKAMI ,  Hidenao IWAI

IPC: G01N33/483 ,  G06T7/00 ,  C12N5/074

CPC classification number: G01N33/4833 ,  C12N5/0696 ,  C12Q1/04 ,  G01N15/1456 ,  G01N2015/1006 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/50 ,  G06T2207/10056 ,  G06T2207/30004

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract translation: 本发明提供了一种确定多能干细胞分化水平的方法，包括确定培养的多能干细胞的平坦度的步骤，其中平坦度是指示。

公开(公告)号：US20160349170A1

公开(公告)日：2016-12-01

申请号：US15037142

申请日：2014-10-17

Applicant: KYOTO UNIVERSITY ,  HAMAMATSU PHOTONICS K.K.

Inventor： Norio NAKATSUJI ,  Norikazu SUGIYAMA ,  Yoshinori MIZUGUCHI ,  Tadashi FUKAMI ,  Hidenao YAMADA ,  Toyohiko YAMAUCHI ,  Yumi KAKUNO

IPC: G01N15/14 ,  G06K9/00

CPC classification number: G01N15/1434 ,  C12M41/36 ,  G01N15/1475 ,  G01N33/5073 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1447 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/00147

Abstract: The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

Abstract translation: 本发明涉及一种细胞评估方法，其特征在于包括获取小细胞团的光程长度图像的获取步骤，在获取的光程长图像内提取细胞核区域的提取步骤，比较步骤 提取的细胞核区域的内侧的光路长度和光路长度，以及基于比较结果来评价细胞是否为干细胞的评价步骤。

公开(公告)号：US20140073002A1

公开(公告)日：2014-03-13

申请号：US14023735

申请日：2013-09-11

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Norikazu SUGIYAMA ,  Tadashi FUKAMI ,  Hidenao IWAI

IPC: G01N33/483

CPC classification number: G01N33/4833 ,  C12N5/0696 ,  C12Q1/04 ,  G01N15/1456 ,  G01N2015/1006 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/50 ,  G06T2207/10056 ,  G06T2207/30004

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract translation: 本发明提供了一种确定多能干细胞分化水平的方法，包括确定培养的多能干细胞的平坦度的步骤，其中平坦度是指示。

公开(公告)号：US20150219543A1

公开(公告)日：2015-08-06

申请号：US14613672

申请日：2015-02-04

Applicant: HAMAMATSU PHOTONICS K.K. ,  Kyoto University

Inventor： Toyohiko YAMAUCHI ,  Tadashi FUKAMI ,  Norikazu SUGIYAMA ,  Hidenao IWAI ,  Yumi KAKUNO ,  Kentaro GOTO ,  Norio NAKATSUJI ,  Kazuhiro AIBA

IPC: G01N15/10 ,  G01N15/00

CPC classification number: G01N15/10 ,  G01N15/00 ,  G01N15/1475 ,  G01N2015/0065 ,  G01N2015/1087 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention relates to a cell evaluation method comprising evaluating a cell differentiation degree based on a cell thickness.

Abstract translation: 本发明涉及一种细胞评估方法，其包括基于细胞厚度评估细胞分化程度。