公开(公告)号：US08184298B2

公开(公告)日：2012-05-22

申请号：US12454660

申请日：2009-05-21

申请人： Gabriel Popescu ,  Zhuo Wang

发明人： Gabriel Popescu ,  Zhuo Wang

IPC分类号： G01B9/02 ,  G01J3/45 ,  G02B21/00

CPC分类号： G01N15/1434 ,  G01N15/0211 ,  G01N15/1459 ,  G01N2015/0038 ,  G01N2015/0222 ,  G01N2015/025 ,  G01N2015/1454 ,  G02B21/0056 ,  G02B21/008 ,  G03H2001/0491 ,  G03H2001/2244 ,  Y10S977/881

摘要： Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.

摘要翻译： 通过透明样品绘制定量相位图的方法和装置。 结合物镜，傅立叶光学元件和可编程的二维相位调制器采用宽带源，以获得图像平面中的幅度和相位信息。 称为傅里叶变换光散射（FTLS）的方法测量样品的角散射光谱。 FTLS结合光学显微镜和光散射来研究不均匀和动态的介质。 FTLS依赖于量化与相干图像场相关联的光学相位和振幅，并将其数值传播到散射平面。 仅由显微镜物镜限制的全角度信息是从极微弱的散射体，如单个微米尺寸的粒子获得的。 流式细胞仪可采用FTLS分选。

公开(公告)号：US08174697B2

公开(公告)日：2012-05-08

申请号：US13245366

申请日：2011-09-26

申请人： John Mitchell ,  Jon Sandberg ,  Karen R. Sandberg, legal representative ,  Dwight A. Sehler

发明人： John Mitchell ,  Jon Sandberg ,  Dwight A. Sehler

IPC分类号： G01N15/02 ,  G01N21/00

CPC分类号： G01N15/1463 ,  G01N15/1459 ,  G01N2015/0038 ,  G01N2015/025 ,  G01N2015/1402

摘要： Described herein is a particle detection system capable of spatially resolving the interaction of particles with a beam of electromagnetic radiation. Using a specific electromagnetic beam cross sectional shape and orientation, the detection sensitivity of a particle detection system can be improved. Also provided are methods for detecting and sizing particles in a manner that has low background signal and allows for spatially resolving the scattering or emission of electromagnetic radiation from particles.

摘要翻译： 这里描述了能够空间分辨粒子与电磁辐射束的相互作用的粒子检测系统。 使用特定的电磁波束截面形状和取向，可以提高粒子检测系统的检测灵敏度。 还提供了以具有低背景信号并且允许空间分辨来自颗粒的电磁辐射的散射或发射的方式来检测和调整颗粒的方法。

公开(公告)号：US08171777B2

公开(公告)日：2012-05-08

申请号：US12212219

申请日：2008-09-17

申请人： Adam Richard Schilffarth

发明人： Adam Richard Schilffarth

IPC分类号： G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N21/64

CPC分类号： G01N15/1429 ,  G01N15/0205 ,  G01N15/1012 ,  G01N15/1031 ,  G01N15/1425 ,  G01N15/1427 ,  G01N15/1459 ,  G01N2015/025 ,  G01N2015/1018 ,  G01N2015/1438 ,  G01N2015/1477

摘要： Methods, storage mediums, and systems for correlating pulses generated from multiple interrogation regions in a flow cytometer to particular particles flowing through the flow cytometer are provided. Embodiments of the methods, storage mediums, and systems include configurations for calibrating a flow cytometer using a calibration particle having a unique signature to determine a time-of-flight for particles flowing through the flow cytometer. Based on the calculated time-of-flight and relative positions of interrogation regions corresponding to collectors of the flow cytometer, the methods, storage mediums, and systems may further include configurations for associating other signal pulses to particles of one or more different particle sets.

摘要翻译： 提供了用于将在流式细胞仪中从多个询问区产生的脉冲相关联的特定颗粒流过流式细胞仪的方法，存储介质和系统。 方法，存储介质和系统的实施方案包括用于使用具有独特标记的校准颗粒来校准流式细胞仪的结构，以确定流过流式细胞仪的颗粒的飞行时间。 基于计算出的与流式细胞仪的收集器对应的询问区域的飞行时间和相对位置，方法，存储介质和系统可进一步包括用于将其它信号脉冲与一个或多个不同粒子集合的粒子相关联的配置。

公开(公告)号：US08125643B2

公开(公告)日：2012-02-28

申请号：US11414839

申请日：2006-05-01

申请人： Frans Ejner Ravn Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Myron Jeppesen

发明人： Frans Ejner Ravn Hansen ,  Martin Glensbjerg ,  Borkur Arnvidarson ,  Jesper Myron Jeppesen

IPC分类号： G01N21/00 ,  G01N33/48

CPC分类号： G01N15/1459 ,  B01L3/5027 ,  G01N15/1463 ,  G01N21/31 ,  G01N21/59 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/76 ,  G01N33/04 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2021/5957 ,  G01N2021/6491

摘要： The present invention relates to a method for the assessment of quantity and quality parameters of biological particles in a liquid analyte material. The method comprises applying a volume of a liquid sample to an exposing domain from which exposing domain electromagnetic signals from the sample in the domain can pass to the exterior, and exposing, onto an array of active detection elements such as CCD-elements, a spatial representation of electromagnetic signals having passed from the domain, the representation being detectable as an intensity by individual active detection elements, under conditions permitting processing of the intensities detected by the array of detection elements during the exposure in such a manner that representations of electromagnetic signals from the biological particles are identified as distinct from representations of electromagnetic signals from background signals. The size of the volume of the liquid sample is sufficiently large to permit the assessment of the quantity and quality parameters to fulfill a predetermined requirement to the statistical quality of the assessment based on substantially one exposure.

摘要翻译： 本发明涉及一种用于评估液体分析物质中生物颗粒的数量和质量参数的方法。 该方法包括将一定体积的液体样品施加到曝光区域，从该区域中的来自样品的曝光区域电磁信号可以从该区域传播到外部，并将其暴露于诸如CCD元件的主动检测元件阵列上，空间 在从暴露期间通过检测元件阵列检测到的强度的处理条件下，可以通过各个有源检测元件检测作为强度的表示作为强度的表示， 生物颗粒被识别为不同于来自背景信号的电磁信号的表示。 液体样品的体积大小足够大以允许评估数量和质量参数以满足基于基本上一次暴露的评估的统计质量的预定要求。

公开(公告)号：US08047055B2

公开(公告)日：2011-11-01

申请号：US12705398

申请日：2010-02-12

申请人： Xiaoliang Wang ,  Jugal K. Agarwal ,  George John Chancellor ,  James Evanstad ,  Anthony E. Hase ,  Sreenath Avula ,  James E. Farnsworth ,  David A. Lieder

发明人： Xiaoliang Wang ,  Jugal K. Agarwal ,  George John Chancellor ,  James Evanstad ,  Anthony E. Hase ,  Sreenath Avula ,  James E. Farnsworth ,  David A. Lieder

IPC分类号： B01D59/44 ,  G01N21/00 ,  B05C1/00

CPC分类号： G01N15/0205 ,  G01N15/1459 ,  G01N2015/025

摘要： A system for measuring size segregated mass concentration of an aerosol. The system includes an electromagnetic radiation source with beam-shaping optics for generation of a beam of electromagnetic radiation, an inlet sample conditioner with adjustable cut-size that selects particles of a specific size range, and an inlet nozzle for passage of an aerosol flow stream. The aerosol flow stream contains particles intersecting the beam of electromagnetic radiation to define an interrogation volume, and scatters the electromagnetic radiation from the interrogation volume. The system also includes a detector for detection of the scattered electromagnetic radiation an integrated signal conditioner coupled to the detector and generating a photometric output, and a processor coupled with the conditioner for conversion of the photometric output and cut-size to a size segregated mass distribution.

摘要翻译： 用于测量气溶胶的尺寸分离质量浓度的系统。 该系统包括具有用于产生电磁辐射束的光束成形光学器件的电磁辐射源，可选择尺寸的入口样品调节器，其选择特定尺寸范围的颗粒，以及用于通过气溶胶流动流的入口喷嘴 。 气溶胶流流含有与电磁辐射束相交的颗粒，以限定询问体积，并散射来自询问体积的电磁辐射。 该系统还包括用于检测散射电磁辐射的检测器，耦合到检测器并产生测光输出的集成信号调节器，以及与调节器耦合的处理器，用于将光度输出和切割尺寸转换成尺寸分离的质量分布 。

公开(公告)号：US07804594B2

公开(公告)日：2010-09-28

申请号：US11934277

申请日：2007-11-02

申请人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

发明人： Giacomo Vacca ,  Norman R. Goldblatt ,  Michael W. Yee

IPC分类号： G01N21/00

CPC分类号： G01N15/0205 ,  G01N15/0227 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/025 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/1493

摘要： A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

摘要翻译： 通过利用激光扫描技术提高流式细胞仪或使用流式细胞仪的血液分析仪的生产量，精度或精度以及吞吐量的方法。 激光扫描涉及通过血液分析仪中的流动样品流扫描激光束。 适用于实施本发明方法的装置包括一个包括光源的光学模块，一个扫描装置，一个透镜或透镜系统，一个流通池，检测器和过滤器; 以及包括前置放大器，模拟信号调节元件，模拟 - 数字转换器，现场可编程门阵列，数字信号处理元件和数据存储元件的电子模块。

公开(公告)号：US20100044586A1

公开(公告)日：2010-02-25

申请号：US12515641

申请日：2007-11-20

申请人： Stefan Duhr ,  Philipp Baaske

发明人： Stefan Duhr ,  Philipp Baaske

IPC分类号： G01J1/58 ,  G01K3/00

CPC分类号： G01N33/6803 ,  B01F13/0079 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0442 ,  B01L2400/0451 ,  B01L2400/0454 ,  C12Q1/68 ,  G01K11/12 ,  G01N15/0205 ,  G01N15/1434 ,  G01N15/1475 ,  G01N21/171 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/6845 ,  G01N2015/0065 ,  G01N2015/0092 ,  G01N2015/025 ,  G01N2021/6439 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0638 ,  Y10T436/143333

摘要： The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).

摘要翻译： 本发明涉及用于快速热光学表征颗粒的方法和装置。 特别地，本发明涉及测量（生物）分子的稳定性，特别是生物分子与分子的相互作用的方法和装置。 进一步（生物）分子，特别是改性（生物）分子，颗粒，珠粒和/或单个（生物）分子，颗粒，珠子的长度/尺寸（例如流体动力学半径）的确定和/或长度/ 尺寸（例如流体动力学半径）。

公开(公告)号：US20090323061A1

公开(公告)日：2009-12-31

申请号：US11710976

申请日：2007-02-27

申请人： Lukas Novotny ,  Filipp Ignatovich

发明人： Lukas Novotny ,  Filipp Ignatovich

IPC分类号： G01N15/02 ,  G01B9/02

CPC分类号： G01N15/1456 ,  G01N15/0211 ,  G01N2015/0038 ,  G01N2015/0088 ,  G01N2015/0233 ,  G01N2015/025 ,  G01N2015/1454 ,  G01N2015/1493

摘要： A nanoparticle sensor is capable of detecting and recognizing single nanoparticles in an aqueous environment. Such sensor may find applications in broad areas of science and technology, from the analysis of diesel engine emissions to the detection of biological warfare agents. Particle detection is based on interferometric detection of multi-color light, scattered by the particle. On the fundamental level, the detected signal has a weaker dependence on particle size (ÿ R3), compared to standard detection methods (ÿ R6). This leads to a significantly larger signal-to-noise ratio for smaller particles. By using a multi-color or white excitation light, particle dielectric properties are probed at different frequencies. This scheme samples the frequency dependence of the particle's polarizability thereby making it possible to predict the composition of the particle material. The detection scheme also employs a heterodyne or pseudoheterodyne detection configuration, which allows it to reduce or eliminate noise contribution from phase variations, which appear in any interferometric measurements.

摘要翻译： 纳米颗粒传感器能够在水性环境中检测和识别单个纳米颗粒。 这种传感器可以在广泛的科学和技术领域应用，从柴油机排放分析到生物战剂的检测。 粒子检测是基于粒子散射的多色光的干涉检测。 在基本水平上，与标准检测方法（ÿR6）相比，检测到的信号对粒度（ÿR3）的依赖性较弱。 这导致较小颗粒的信噪比显着更大。 通过使用多色或白色激发光，探测不同频率的粒子介电特性。 该方案对粒子极化率的频率依赖性进行了采样，从而可以预测粒子材料的组成。 检测方案还采用外差或伪外差检测配置，其允许其减少或消除出现在任何干涉测量中的相位变化的噪声贡献。

公开(公告)号：US20090071225A1

公开(公告)日：2009-03-19

申请号：US12212219

申请日：2008-09-17

申请人： Adam Richard Schilffarth

发明人： Adam Richard Schilffarth

IPC分类号： G01N15/00 ,  G01N21/05

CPC分类号： G01N15/1429 ,  G01N15/0205 ,  G01N15/1012 ,  G01N15/1031 ,  G01N15/1425 ,  G01N15/1427 ,  G01N15/1459 ,  G01N2015/025 ,  G01N2015/1018 ,  G01N2015/1438 ,  G01N2015/1477

摘要： Methods, storage mediums, and systems for correlating pulses generated from multiple interrogation regions in a flow cytometer to particular particles flowing through the flow cytometer are provided. Embodiments of the methods, storage mediums, and systems include configurations for calibrating a flow cytometer using a calibration particle having a unique signature to determine a time-of-flight for particles flowing through the flow cytometer. Based on the calculated time-of-flight and relative positions of interrogation regions corresponding to collectors of the flow cytometer, the methods, storage mediums, and systems may further include configurations for associating other signal pulses to particles of one or more different particle sets.

摘要翻译： 提供了用于将在流式细胞仪中从多个询问区产生的脉冲相关联的特定颗粒流过流式细胞仪的方法，存储介质和系统。 方法，存储介质和系统的实施方案包括用于使用具有独特标记的校准颗粒来校准流式细胞仪的结构，以确定流过流式细胞仪的颗粒的飞行时间。 基于计算出的与流式细胞仪的收集器对应的询问区域的飞行时间和相对位置，方法，存储介质和系统可进一步包括用于将其它信号脉冲与一个或多个不同粒子集合的粒子相关联的配置。

公开(公告)号：US20080008623A1

公开(公告)日：2008-01-10

申请号：US11706458

申请日：2007-02-14

申请人： Wayne Reed

发明人： Wayne Reed

IPC分类号： G01N35/10

CPC分类号： G01N35/1097 ,  G01N15/0211 ,  G01N15/14 ,  G01N15/1434 ,  G01N21/49 ,  G01N21/51 ,  G01N21/53 ,  G01N30/02 ,  G01N30/74 ,  G01N35/08 ,  G01N35/085 ,  G01N2011/0046 ,  G01N2015/0092 ,  G01N2015/0216 ,  G01N2015/025 ,  G01N2015/0693 ,  G01N2021/4711 ,  G01N2021/4716 ,  G01N2021/4719 ,  G01N2021/513 ,  G01N2201/0218 ,  B01D15/34

摘要： The present invention provides an automatic sampling and dilution apparatus for use in a polymer analysis system. The apparatus comprises (a) a primary mixing chamber; (b) a primary pump capable of continuously withdrawing a variable viscosity liquid from a reactor at a selectable, fixed withdrawal rate over a varying viscosity range of about 50 to about 5,000,000 centipoise (cP) for continuously conveying the variable viscosity polymer-containing liquid into the primary mixing chamber; (c) a first dilution pump for continuously delivering a first dilution solvent into the primary mixing chamber at a selectable, fixed flow rate to mix with the variable viscosity liquid in the mixing chamber and thereby form a diluted polymer-containing liquid therein; and (d) a secondary pump for continuously conveying the diluted polymer-containing liquid into a flow-through detector. A polymer analysis system utilizing the automatic sampling and dilution apparatus is also provided.

摘要翻译： 本发明提供一种用于聚合物分析系统的自动取样和稀释装置。 该装置包括（a）主混合室; （b）能够以约50至约5,000,000厘泊（cP）的变化的粘度范围以可选择的固定的抽出速率从反应器连续抽出可变粘度液体的初级泵，用于将可变粘度的含聚合物的液体连续地输送到 主混合室; （c）第一稀释泵，用于以可选择的固定流速连续地将第一稀释溶剂输送到主混合室中，以与混合室中的可变粘度液体混合，从而在其中形成稀释的含聚合物的液体; 和（d）用于将稀释的含聚合物的液体连续输送到流通检测器中的次级泵。 还提供了利用自动取样和稀释装置的聚合物分析系统。