公开(公告)号：US20210322970A1

公开(公告)日：2021-10-21

申请号：US16605267

申请日：2018-07-17

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  John Lahmann ,  Alexander Govyadinov

IPC: B01L3/02 ,  B01F5/00 ,  B01F15/02

Abstract: An example device includes a first droplet ejector including a first nozzle to eject droplets of a first fluid, a second droplet ejector including a second nozzle to eject droplets of a second fluid, and a target medium. The example device further includes a mixing volume positioned between the first and second droplet ejectors and the target medium. The mixing volume is to receive the droplets of the first fluid and the droplets of the second fluid, provide mixing of the droplets of the first fluid and the droplets of the second fluid, and provide a mixture to the target medium.

公开(公告)号：US11547993B2

公开(公告)日：2023-01-10

申请号：US16608289

申请日：2018-07-17

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  John Lahmann ,  Alexander Govyadinov ,  Diane R Hammerstad

IPC: B41J2/14 ,  B41J2/175 ,  B01L3/02 ,  B41J2/045

Abstract: An example device includes a droplet ejector including a nozzle to eject droplets of a fluid and a target medium to receive the droplets of the fluid. The target medium is separated from the droplet ejector by a gap to be traversed by the droplets. The example device further includes a frame affixing the target medium to the droplet ejector. The target medium is immovably held with respect to the droplet ejector.

公开(公告)号：US20250135458A1

公开(公告)日：2025-05-01

申请号：US19009745

申请日：2025-01-03

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Si-lam Choy ,  Hilary Ely ,  John Lahmann

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6844

Abstract: Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.

公开(公告)号：US12233415B2

公开(公告)日：2025-02-25

申请号：US17417306

申请日：2019-04-29

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Si-lam Choy ,  Hilary Ely ,  John Lahmann

IPC: B01L3/00 ,  A61L27/16 ,  A61L27/56 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B01L7/00 ,  B01L9/00 ,  B23Q17/24 ,  C07K14/705 ,  C08F220/56 ,  C08L33/26 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N1/31 ,  G01N21/17 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N21/77 ,  G01N21/78 ,  G01N27/414 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74 ,  G01N35/00 ,  G01N35/10 ,  G06T7/00 ,  G06T7/90 ,  H10K10/46 ,  H10K85/00 ,  H10K85/20

Abstract: Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.

公开(公告)号：US11931738B2

公开(公告)日：2024-03-19

申请号：US17045873

申请日：2018-11-22

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： John Lahmann ,  Pavel Kornilovich ,  Silam J Choy

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2300/0645 ,  B01L2400/0406 ,  B01L2400/0442

Abstract: An example method includes providing fluid to a chamber. The chamber feeds a first channel terminating at a first droplet ejector and a second channel terminating at a second droplet ejector. The method further includes sequencing ejection of droplets at the first droplet ejector and the second droplet ejector to induce negative pressure to provide a sequenced output flow of the fluid through the first channel to a first target microfluidic network and through the second channel to a second target microfluidic network, and controlling the first and second target microfluidic networks to perform an analytical process with the fluid.

公开(公告)号：US11325380B2

公开(公告)日：2022-05-10

申请号：US16605255

申请日：2018-07-17

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  Alexander Govyadinov ,  John Lahmann ,  Paul J. Benning

IPC: B41J2/14 ,  B41J2/175

Abstract: An example device includes a first droplet ejector including a first nozzle to eject droplets of a first fluid, and a first target medium positioned relative to the first droplet ejector to receive the droplets of the first fluid from the first droplet ejector. The example device further includes a second droplet ejector in fluid communication with the first target medium to receive a second fluid from the first target medium. The second droplet ejector includes a second nozzle to eject droplets of the second fluid.

公开(公告)号：US20210331468A1

公开(公告)日：2021-10-28

申请号：US16605255

申请日：2018-07-17

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  Alexander Govyadinov ,  John Lahmann ,  Paul J. Benning

IPC: B41J2/14 ,  B41J2/175

Abstract: An example device includes a first droplet ejector including a first nozzle to eject droplets of a first fluid, and a first target medium positioned relative to the first droplet ejector to receive the droplets of the first fluid from the first droplet ejector. The example device further includes a second droplet ejector in fluid communication with the first target medium to receive a second fluid from the first target medium. The second droplet ejector includes a second nozzle to eject droplets of the second fluid.