公开(公告)号：US08163495B2

公开(公告)日：2012-04-24

申请号：US11853604

申请日：2007-09-11

申请人： Hans-Jorg Buhring ,  Reiner Lammers ,  Sabrina Treml ,  Venkata Lokesh Battula

发明人： Hans-Jorg Buhring ,  Reiner Lammers ,  Sabrina Treml ,  Venkata Lokesh Battula

IPC分类号： G01N33/53 ,  C12N5/00 ,  C12N5/071

CPC分类号： G01N33/577 ,  A61K35/28 ,  C07K16/28 ,  C07K16/2863 ,  C07K16/3015 ,  C07K16/32 ,  G01N33/6887 ,  G01N2333/705

摘要： The present invention concerns antibodies produced from hybridoma cell lines chosen from the group comprising W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, for isolating and/or identifying homogenous mesenchymal stem cells. Furthermore a method is presented with which mesenchymal stem cells from adult primary tissue, for example bone marrow, can be identified and isolated with a high level of purity.

摘要翻译： 本发明涉及从选自W8B2，W1C3，W7C6，W5C4,24D2,28D4，HEK-3D6，W4A5，W3D5，W5C5,9A3G9,5BB1，F9-3C2F1,399D5的杂交瘤细胞系产生的抗体，用于分离和 /或鉴定均质间充质干细胞。 此外，可以以高纯度鉴定和分离来自成年原代组织（例如骨髓）的间充质干细胞的方法。

公开(公告)号：US20080075699A1

公开(公告)日：2008-03-27

申请号：US11853604

申请日：2007-09-11

申请人： Hans-Jorg Buhring ,  Reiner Lammers ,  Sabrina Treml ,  Venkata Battula

发明人： Hans-Jorg Buhring ,  Reiner Lammers ,  Sabrina Treml ,  Venkata Battula

IPC分类号： A61K35/12 ,  C12N5/08

CPC分类号： G01N33/577 ,  A61K35/28 ,  C07K16/28 ,  C07K16/2863 ,  C07K16/3015 ,  C07K16/32 ,  G01N33/6887 ,  G01N2333/705

摘要： The present invention concerns antibodies produced from hybridoma cell lines chosen from the group comprising W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, for isolating and/or identifying homogenous mesenchymal stem cells. Furthermore a method is presented with which mesenchymal stem cells from adult primary tissue, for example bone marrow, can be identified and isolated with a high level of purity.

摘要翻译： 本发明涉及从选自W8B2，W1C3，W7C6，W5C4,24D2,28D4，HEK-3D6，W4A5，W3D5，W5C5,9A3G9,5BB1，F9-3C2F1,399D5的杂交瘤细胞系产生的抗体，用于分离和 /或鉴定均质间充质干细胞。 此外，可以以高纯度鉴定和分离来自成年原代组织（例如骨髓）的间充质干细胞的方法。

公开(公告)号：US07364863B2

公开(公告)日：2008-04-29

申请号：US11077486

申请日：2005-03-09

申请人： Hans-Jorg Buhring ,  Reiner Lammers ,  Wichard Vogel

发明人： Hans-Jorg Buhring ,  Reiner Lammers ,  Wichard Vogel

IPC分类号： G01N33/53 ,  C12N5/00 ,  C07K16/00 ,  C12P21/08

CPC分类号： G01N33/6887 ,  C07K16/28 ,  G01N33/56966

摘要： The invention relates to a monoclonal antibody, or fragments thereof, for isolating and/or identifying mesenchymal stem cells. In this connection, the antibody, or fragments thereof, bind to an antigen which is the same as that bound to by an antibody which is produced by the hybridoma cell line W8B2, which was deposited on 14.08.2002 in the DSMZ [German collection of microorganisms and cell cultures] under the number DSM ACC2567.

摘要翻译： 本发明涉及用于分离和/或鉴定间充质干细胞的单克隆抗体或其片段。 在这方面，抗体或其片段与抗原结合，该抗原与通过由DSMZ [德国专利公开说明书]中保藏的杂交瘤细胞系W8B2产生的抗体相结合 微生物和细胞培养物]，号码为DSM ACC2567。

公开(公告)号：US09052316B2

公开(公告)日：2015-06-09

申请号：US12980092

申请日：2010-12-28

申请人： Hans-Joerg Buehring ,  Sabrina Treml ,  Reiner Lammers

发明人： Hans-Joerg Buehring ,  Sabrina Treml ,  Reiner Lammers

IPC分类号： G01N33/531 ,  G01N33/569 ,  C07K16/28 ,  C07K16/40 ,  C12N5/0775

CPC分类号： G01N33/56966 ,  C07K16/2803 ,  C07K16/40 ,  C12N5/0663 ,  G01N33/531

摘要： The present invention relates to a method for isolating and/or identifying stem cells having adipocytic, chondrocytic and pancreatic differentiation potential, wherein an antibody is used that binds to the antigen TNAP, alone or in combination with an antibody that binds to the cell surface antigen CD56. The invention also relates to stem cells isolated by the method according to the invention for treating defects or damages or diseases in bone or cartilage of a patient in need thereof.

摘要翻译： 本发明涉及用于分离和/或鉴定具有脂肪细胞，软骨细胞和胰腺分化潜能的干细胞的方法，其中使用与抗原TNAP单独或与结合细胞表面抗原的抗体结合的抗体 CD56。 本发明还涉及通过根据本发明的方法分离的用于治疗有需要的患者的骨或软骨中的缺陷或损伤或疾病的干细胞。

公开(公告)号：US20110117575A1

公开(公告)日：2011-05-19

申请号：US12980092

申请日：2010-12-28

申请人： Hans-Joerg Buehring ,  Sabrina Treml ,  Reiner Lammers

发明人： Hans-Joerg Buehring ,  Sabrina Treml ,  Reiner Lammers

IPC分类号： G01N33/566 ,  C12N5/077 ,  C12N5/074

CPC分类号： G01N33/56966 ,  C07K16/2803 ,  C07K16/40 ,  C12N5/0663 ,  G01N33/531

摘要： The present invention relates to a method for isolating and/or identifying stem cells having adipocytic, chondrocytic and pancreatic differentiation potential, wherein an antibody is used that binds to the antigen TNAP, alone or in combination with an antibody that binds to the cell surface antigen CD56. The invention also relates to stem cells isolated by the method according to the invention for treating defects or damages or diseases in bone or cartilage of a patient in need thereof.

摘要翻译： 本发明涉及用于分离和/或鉴定具有脂肪细胞，软骨细胞和胰腺分化潜能的干细胞的方法，其中使用与抗原TNAP单独或与结合细胞表面抗原的抗体结合的抗体 CD56。 本发明还涉及通过根据本发明的方法分离的用于治疗有需要的患者的骨或软骨中的缺陷或损伤或疾病的干细胞。

公开(公告)号：US06331555B1

公开(公告)日：2001-12-18

申请号：US09363237

申请日：1999-07-27

申请人： Klaus P. Hirth ,  Elaina Mann ,  Laura K. Shawyer ,  Axel Ullrich ,  Istvan Szekely ,  Tamas Bajor ,  Janis Haimichael ,  Laszlo Orfi ,  Alex Levitzki ,  Aviv Gazit ,  Peng Cho Tang ,  Reiner Lammers

发明人： Klaus P. Hirth ,  Elaina Mann ,  Laura K. Shawyer ,  Axel Ullrich ,  Istvan Szekely ,  Tamas Bajor ,  Janis Haimichael ,  Laszlo Orfi ,  Alex Levitzki ,  Aviv Gazit ,  Peng Cho Tang ,  Reiner Lammers

IPC分类号： A61K3142

CPC分类号： A61K31/135 ,  A61K31/12 ,  A61K31/13 ,  A61K31/415 ,  A61K31/42

摘要： The present invention concerns compounds which can inhibit platelet derived growth factor receptor (PDGF-R) activity, preferably such compounds also inhibit the activity other members of the PDGF-R super family and are selective for members of the PDGF-R super family. The PDGF-R super family includes PDGF-R and PDGF-R related kinases Flt, and KDR. The featured compounds are active on cell cultures to reduce the activity of the PDGF-R and preferably one or more PDGF-R related kinases. An example of a featured compound, A10 (see FIG. 1a), and its ability to inhibit growth of tumor cells in vivo is described below. Using the present application as guide other compounds able to inhibit PDGF-R and preferably Flt and/or KDR can be obtained. Such compounds are preferably used to treat patients suffering from cell proliferative disorders characterized by inappropriate PDGF-R activity.

摘要翻译： 本发明涉及可以抑制血小板衍生生长因子受体（PDGF-R）活性的化合物，优选地，这些化合物也抑制PDGF-R超家族的其他成员的活性，并且对PDGF-R超家族的成员是选择性的。 PDGF-R超家族包括PDGF-R和PDGF-R相关的激酶Flt和KDR。 特征化合物在细胞培养物中是有活性的，以降低PDGF-R，优选一种或多种PDGF-R相关激酶的活性。 以下描述特征化合物A10（参见图1a）及其抑制体内肿瘤细胞生长的能力的实例。 使用本申请作为指导，可以获得能够抑制PDGF-R，优选Flt和/或KDR的其它化合物。 这些化合物优选用于治疗患有以不适当的PDGF-R活性为特征的细胞增殖性疾病的患者。

公开(公告)号：US5990141A

公开(公告)日：1999-11-23

申请号：US370574

申请日：1995-01-06

申请人： Klaus Peter Hirth ,  Donna Pruess Schwartz ,  Elaina Mann ,  Laura Kay Shawver ,  Gyorgi Keri ,  Istvan Szekely ,  Tamas Bajor ,  Janis Haimichael ,  Laszlo Orfi ,  Alex Levitzki ,  Aviv Gazit ,  Axel Ullrich ,  Reiner Lammers ,  Fairooz F. Kabbinavar ,  Dennis Slamon ,  Peng Cho Tang

发明人： Klaus Peter Hirth ,  Donna Pruess Schwartz ,  Elaina Mann ,  Laura Kay Shawver ,  Gyorgi Keri ,  Istvan Szekely ,  Tamas Bajor ,  Janis Haimichael ,  Laszlo Orfi ,  Alex Levitzki ,  Aviv Gazit ,  Axel Ullrich ,  Reiner Lammers ,  Fairooz F. Kabbinavar ,  Dennis Slamon ,  Peng Cho Tang

IPC分类号： A61K9/00 ,  A61K31/165 ,  A61K31/167 ,  A61K31/275 ,  A61K31/277 ,  A61K31/38 ,  A61K31/40 ,  A61K31/415 ,  A61K31/42 ,  A61K31/421 ,  A61K31/425 ,  A61K31/44 ,  A61K31/47 ,  A61K31/495 ,  A61K31/505 ,  A61K31/535 ,  A61K45/06 ,  A61K47/10 ,  A61K47/26 ,  C07C255/23 ,  C07D231/38 ,  C07D239/91 ,  C07D241/38 ,  C07D241/42 ,  C07D487/04 ,  C07D498/04 ,  G01N33/50

CPC分类号： C07D231/38 ,  A61K31/165 ,  A61K31/167 ,  A61K31/275 ,  A61K31/277 ,  A61K31/38 ,  A61K31/40 ,  A61K31/415 ,  A61K31/42 ,  A61K31/421 ,  A61K31/425 ,  A61K31/44 ,  A61K31/47 ,  A61K31/495 ,  A61K31/505 ,  A61K31/535 ,  A61K45/06 ,  A61K47/10 ,  A61K47/26 ,  A61K9/0019 ,  C07C255/23 ,  C07D239/91 ,  C07D241/38 ,  C07D241/42 ,  C07D487/04 ,  G01N33/5011 ,  Y10S977/904 ,  Y10S977/911 ,  Y10S977/912 ,  Y10S977/915

摘要： The present invention concerns compounds which can inhibit platelet derived growth factor receptor (PDGF-R) activity, preferably such compounds also inhibit the activity other members of the PDGF-R super family and are selective for members of the PDGF-R super family. The PDGF-R super family includes PDGF-R and PDGF-R related kinases Flt, and KDR. The featured compounds are active on cell cultures to reduce the activity of the PDGF-R and preferably one or more PDGF-R related kinases. An example of a featured compound, A10 (see FIG. 1a), and its ability to inhibit growth of tumor cells in vivo is described below. Using the present application as guide other compounds able to inhibit PDGF-R and preferably Flt and/or KDR can be obtained. Such compounds are preferably used to treat patients suffering from cell proliferative disorders characterized by inappropriate PDGF-R activity.

摘要翻译： 本发明涉及可以抑制血小板衍生生长因子受体（PDGF-R）活性的化合物，优选地，这些化合物也抑制PDGF-R超家族的其他成员的活性，并且对PDGF-R超家族的成员是选择性的。 PDGF-R超家族包括PDGF-R和PDGF-R相关的激酶Flt和KDR。 特征化合物在细胞培养物中是有活性的，以降低PDGF-R，优选一种或多种PDGF-R相关激酶的活性。 下面描述特征化合物A10（参见图1a）及其抑制体内肿瘤细胞生长的能力的实例。 使用本申请作为指导，可以获得能够抑制PDGF-R，优选Flt和/或KDR的其它化合物。 这些化合物优选用于治疗患有以不适当的PDGF-R活性为特征的细胞增殖性疾病的患者。

公开(公告)号：US5958959A

公开(公告)日：1999-09-28

申请号：US457051

申请日：1995-06-01

申请人： Klaus Peter Hirth ,  Donna Pruess Schwartz ,  Elaina Mann ,  Laura Kay Shawver ,  Axel Ullrich ,  Reiner Lammers

发明人： Klaus Peter Hirth ,  Donna Pruess Schwartz ,  Elaina Mann ,  Laura Kay Shawver ,  Axel Ullrich ,  Reiner Lammers

IPC分类号： A61K9/00 ,  A61K31/165 ,  A61K31/167 ,  A61K31/275 ,  A61K31/277 ,  A61K31/38 ,  A61K31/40 ,  A61K31/415 ,  A61K31/42 ,  A61K31/421 ,  A61K31/425 ,  A61K31/44 ,  A61K31/47 ,  A61K31/495 ,  A61K31/505 ,  A61K31/535 ,  A61K45/06 ,  A61K47/10 ,  A61K47/26 ,  C07C255/23 ,  C07D231/38 ,  C07D239/91 ,  C07D241/38 ,  C07D241/42 ,  C07D487/04 ,  C07D498/04 ,  G01N33/50

CPC分类号： C07D231/38 ,  A61K31/165 ,  A61K31/167 ,  A61K31/275 ,  A61K31/277 ,  A61K31/38 ,  A61K31/40 ,  A61K31/415 ,  A61K31/42 ,  A61K31/421 ,  A61K31/425 ,  A61K31/44 ,  A61K31/47 ,  A61K31/495 ,  A61K31/505 ,  A61K31/535 ,  A61K45/06 ,  A61K47/10 ,  A61K47/26 ,  A61K9/0019 ,  C07C255/23 ,  C07D239/91 ,  C07D241/38 ,  C07D241/42 ,  C07D487/04 ,  G01N33/5011 ,  Y10S977/904 ,  Y10S977/911 ,  Y10S977/912 ,  Y10S977/915

摘要： The present invention concerns compounds which can inhibit platelet derived growth factor receptor (PDGF-R) activity, preferably such compounds also inhibit the activity other members of the PDFG-R superfamily and are selective for members of the PDGF-R superfamily. The PDGF-R superfamily includes PDGF-R and PDGF-R related kinases Flt, and KDR. The featured compounds are active on cell cultures to reduce the activity of the PDGF-R and preferably one or more PDGF-R related kinases. An example of a featured compound, A10 (see FIG. 1a), and its ability to inhibit growth of tumor cells in vivo is described below. Using the present application as guide other compounds able to inhibit PDGF-R and preferably Flt and/or KDR can be obtained. Such compounds are preferably used to treat patients suffering from cell proliferative disorders characterized by inappropriate PDGF-R activity.

摘要翻译： 本发明涉及可以抑制血小板衍生生长因子受体（PDGF-R）活性的化合物，优选地，这些化合物还抑制PDFG-R超家族的其他成员的活性，并且对PDGF-R超家族的成员是选择性的。 PDGF-R超家族包括PDGF-R和PDGF-R相关的激酶Flt和KDR。 特征化合物在细胞培养物中是有活性的，以降低PDGF-R，优选一种或多种PDGF-R相关激酶的活性。 以下描述特征化合物A10（参见图1a）及其抑制体内肿瘤细胞生长的能力的实例。 使用本申请作为指导，可以获得能够抑制PDGF-R，优选Flt和/或KDR的其它化合物。 这些化合物优选用于治疗患有以不适当的PDGF-R活性为特征的细胞增殖性疾病的患者。

公开(公告)号：US5932602A

公开(公告)日：1999-08-03

申请号：US456735

申请日：1995-06-01

申请人： Klaus Peter Hirth ,  Laura Kay Shawver ,  Gyorgi Keri ,  Istvan Szekely ,  Janis Haimichael ,  Axel Ullrich ,  Reiner Lammers

发明人： Klaus Peter Hirth ,  Laura Kay Shawver ,  Gyorgi Keri ,  Istvan Szekely ,  Janis Haimichael ,  Axel Ullrich ,  Reiner Lammers

IPC分类号： A61K9/00 ,  A61K31/165 ,  A61K31/167 ,  A61K31/275 ,  A61K31/277 ,  A61K31/38 ,  A61K31/40 ,  A61K31/415 ,  A61K31/42 ,  A61K31/421 ,  A61K31/425 ,  A61K31/44 ,  A61K31/47 ,  A61K31/495 ,  A61K31/505 ,  A61K31/535 ,  A61K45/06 ,  A61K47/10 ,  A61K47/26 ,  C07C255/23 ,  C07D231/38 ,  C07D239/91 ,  C07D241/38 ,  C07D241/42 ,  C07D487/04 ,  C07D498/04 ,  G01N33/50 ,  A61K31/175

CPC分类号： C07D231/38 ,  A61K31/165 ,  A61K31/167 ,  A61K31/275 ,  A61K31/277 ,  A61K31/38 ,  A61K31/40 ,  A61K31/415 ,  A61K31/42 ,  A61K31/421 ,  A61K31/425 ,  A61K31/44 ,  A61K31/47 ,  A61K31/495 ,  A61K31/505 ,  A61K31/535 ,  A61K45/06 ,  A61K47/10 ,  A61K47/26 ,  A61K9/0019 ,  C07C255/23 ,  C07D239/91 ,  C07D241/38 ,  C07D241/42 ,  C07D487/04 ,  G01N33/5011 ,  Y10S977/904 ,  Y10S977/911 ,  Y10S977/912 ,  Y10S977/915

摘要： The present invention concerns compounds which can inhibit platelet derived growth factor receptor (PDGF-R) activity, preferably such compounds also inhibit the activity other members of the PDGF-R super family and are selective for members of the PDGF-R super family. The PDGF-R super family includes PDGF-R and PDGF-R related kinases Flt, and KDR. The featured compounds are active on cell cultures to reduce the activity of the PDGF-R and preferably one or more PDGF-R related kinases. An example of a featured compound, A10 (see FIG. 1a), and its ability to inhibit growth of tumor cells in vivo is described below. Using the present application as guide other compounds able to inhibit PDGF-R and preferably Flt and/or KDR can be obtained. Such compounds are preferably used to treat patients suffering from cell proliferative disorders characterized by inappropriate PDGF-R activity.

公开(公告)号：US20050214873A1

公开(公告)日：2005-09-29

申请号：US11077486

申请日：2005-03-09

申请人： Hans-Joerg Buehring ,  Reiner Lammers ,  Wichard Vogel

发明人： Hans-Joerg Buehring ,  Reiner Lammers ,  Wichard Vogel

IPC分类号： C07K16/28 ,  C12N5/06 ,  C12N5/08 ,  G01N33/53 ,  G01N33/567 ,  G01N33/569 ,  G01N33/68

CPC分类号： G01N33/6887 ,  C07K16/28 ,  G01N33/56966

摘要： The invention relates to a monoclonal antibody, or fragments thereof, for isolating and/or identifying mesenchymal stem cells. In this connection, the antibody, or fragments thereof, bind to an antigen which is the same as that bound to by an antibody which is produced by the hybridoma cell line W8B2, which was deposited on 14.08.2002 in the DSMZ [German collection of microorganisms and cell cultures] under the number DSM ACC2567.

摘要翻译： 本发明涉及用于分离和/或鉴定间充质干细胞的单克隆抗体或其片段。 在这方面，抗体或其片段与抗原结合，该抗原与通过由DSMZ [德国专利公开说明书]中保藏的杂交瘤细胞系W8B2产生的抗体相结合 微生物和细胞培养物]，号码为DSM ACC2567。