摘要:
The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.
摘要:
The present invention provides a protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for proteins of the amino acid sequences as shown in SEQ ID NOS: 4 to 6), and a process for industrially advantageously producing a compound that inhibits HMG-CoA reductase and has an action to decrease serum cholesterol, using DNA encoding the protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for DNA encoding the protein comprising the amino acid sequence as shown in SEQ ID NO: 4).
摘要翻译:本发明提供了包含SEQ ID NO:1〜3中任一项所示的氨基酸序列的蛋白质(除了SEQ ID NO:4〜6所示的氨基酸序列的蛋白质)和工业上的工序 有利地产生抑制HMG-CoA还原酶并具有降低血清胆固醇的作用的化合物,使用编码包含SEQ ID NO:1至3中任一项所示的氨基酸序列的蛋白的DNA(除了编码包含 如SEQ ID NO:4所示的氨基酸序列)。
摘要:
The present invention relates to processes for producing compounds by using a protein derived from a microorganism belonging to the genus Bacillus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and R2 represents a substituted or unsubstituted alkyl or substituted or unsubstituted aryl, or a ring-closed lactone from thereof.
摘要:
The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.
摘要:
The present invention provides a protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for proteins of the amino acid sequences as shown in SEQ ID NOS: 4 to 6), and a process for industrially advantageously producing a compound that inhibits HMG-CoA reductase and has an action to decrease serum cholesterol, using DNA encoding the protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for DNA encoding the protein comprising the amino acid sequence as shown in SEQ ID NO: 4).
摘要翻译:本发明提供了包含SEQ ID NO:1〜3中任一项所示的氨基酸序列的蛋白质(除了SEQ ID NO:4〜6所示的氨基酸序列的蛋白质)和工业上的工序 有利地产生抑制HMG-CoA还原酶并具有降低血清胆固醇的作用的化合物,使用编码包含SEQ ID NO:1至3中任一项所示的氨基酸序列的蛋白的DNA(除了编码包含 如SEQ ID NO:4所示的氨基酸序列)。
摘要:
The present invention relates to a protein derived from a microorganism belonging to the genus Bacillus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and R2 represents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl, or a ring-closed lactone form thereof; a DNA encoding the protein; and a recombinant DNA comprising the DNA.
摘要:
According to the present invention, it is possible to provide a microorganism belonging to the genus Corynebacterium, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.
摘要翻译:根据本发明,可以提供属于棒状杆菌属的微生物,其中(1)具有SEQ ID NO:1〜3或(2)中任一项所示的氨基酸序列的蛋白质的活性, 与SEQ ID NO:1〜3中任一项所示的氨基酸序列具有80%以上的同源性的蛋白质,并且具有与具有下述任一项所示的氨基酸序列的蛋白质的活性基本上相同的活性 SEQ ID NO:1至3已经被还原或丧失,并且其中(3)具有SEQ ID NO:4所示氨基酸序列的蛋白质的活性,或(4)具有80%同源性的蛋白质或 更多地与SEQ ID NO:4所示的氨基酸序列相比,具有与SEQ ID NO:4所示的氨基酸序列的蛋白质的活性基本上相同的活性已被减少或丧失, 使用微生物等的L-谷氨酰胺。
摘要:
The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.
摘要:
The present invention provides a process for producing a dipeptide which comprises: allowing an enzyme source and a diketopiperazine wherein one or two kinds of α-amino acids are condensed with each other to be present in an aqueous medium, said enzyme source being a culture of a microorganism having the ability to produce a dipeptide from a diketopiperazine wherein two kinds of α-amino acids are condensed with each other or a treated matter of the culture; allowing the dipeptide to form and accumulate in the aqueous medium; and recovering the dipeptide from the aqueous medium (provided that the case in which the diketopiperazine is a diketopiperazine wherein aspartic acid and phenylalanine are condensed with each other and the dipeptide is aspartylphenylalanine is excluded).
摘要:
The present invention relates to a process for producing a compound (II-a) or a compound (II-b), each of which is a hydroxylated product of a compound represented by the formula (I-a) (hereinafter referred to as compound (I-a)): wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and R2 represents a substituted or unsubstituted alkyl, or a substituted or unsubstituted aryl; or a ring-closed lactone form thereof (hereinafter referred to as compound (I-b)). wherein the process comprises: treating the compound (I-a) or compound (I-b) in an aqueous medium comprising with a microorganism acting to hydroxylate compound (I-a) or compound (I-b), having no ability to sporulate and showing no hyphal growth, a culture of the microorganism, or a treated product of the culture, as an enzyme source; and collecting a hydroxylated product of compound (I-a) or compound (I-b) from the aqueous medium.