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1.发明授权公开(公告)号：US07704752B2
公开(公告)日：2010-04-27
申请号：US12144277
申请日：2008-06-23
IPC分类号： G01N21/76 , G01N33/533 , C12Q1/28 , C07F5/02 , C07D321/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O− is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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公开(公告)号：US20070190526A1
公开(公告)日：2007-08-16
申请号：US11706547
申请日：2007-02-15
申请人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
发明人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
CPC分类号： C12Q1/6806
摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.
摘要翻译： 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。
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3.发明授权Processing and synthetic intermediates for preparing N-arylacridancarboxylic acid derivatives 失效用于制备N-芳基吖啶羧酸衍生物的加工和合成中间体公开(公告)号：US06410732B2
公开(公告)日：2002-06-25
申请号：US09770015
申请日：2001-01-25
IPC分类号： C07D21902
CPC分类号： C07D417/04 , C07D219/04 , C07D219/06
摘要： Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermediates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了合成方法和中间体用于制备N-芳基吖啶羧酸衍生物。 衍生物是酯，硫酯，酰胺和磺酰亚胺。 该方法的一个关键特征是通过在吖啶环的氮原子和另一芳族或杂芳族环化合物的碳原子之间形成键而制备N-芳基取代的中间体。 芳基化反应由钯催化剂催化。 N-芳基吖啶羧酸衍生物可用于生产光的方法和用于过氧化物酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。
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公开(公告)号：US08137990B2
公开(公告)日：2012-03-20
申请号：US12541245
申请日：2009-08-14
IPC分类号： G01N33/533 , G01N21/76 , C07D321/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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5.发明申请公开(公告)号：US20080261318A1
公开(公告)日：2008-10-23
申请号：US12144277
申请日：2008-06-23
IPC分类号： G01N33/84
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O - 的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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公开(公告)号：US20100055800A1
公开(公告)日：2010-03-04
申请号：US12541245
申请日：2009-08-14
IPC分类号： G01N33/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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公开(公告)号：US07390670B2
公开(公告)日：2008-06-24
申请号：US10371053
申请日：2003-02-20
IPC分类号： G01N21/76 , G01N33/533 , C12Q1/28 , C07F5/02 , C07D321/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig—OH or Sig—O− is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O - 的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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8.发明授权公开(公告)号：US06919463B2
公开(公告)日：2005-07-19
申请号：US10600928
申请日：2003-06-20
IPC分类号： C07D305/00 , C07D321/00 , C07F5/02 , C12N9/96 , C12Q1/26 , G01N20060101 , G01N21/76 , G01N33/53 , G01N33/533 , G01N33/534
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Compounds useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is an aromatic or heteroaromatic ring group, B is a boron atom, and R5 and R6 are independently selected from hydrogen and lower alkyl groups and can be joined together as a straight or branched alkylene chain forming a five or six-membered ring. A detectable product compound of the formula Sig-OH Is produced and detected by measuring color, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The compounds can be used for detection in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的化合物，其中下式的信号化合物与过氧化物反应。 Sig是芳族或杂芳族环基团，B是硼原子，R 5和R 6独立地选自氢和低级烷基，并且可以以 形成五元或六元环的直链或支链亚烷基链。 通过测量颜色，荧光，化学发光或生物发光产生和检测式Sig-OH的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该化合物可用于检测过氧化物或过氧化物产生酶的检测中，以及使用酶标记的特异性结合对的检测。
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9.发明授权Uses of improved polymer-supported photosensitizers in the generation of singlet oxygen 有权改进的聚合物负载的光敏剂在产生单线态氧中的应用公开(公告)号：US06774249B2
公开(公告)日：2004-08-10
申请号：US09965046
申请日：2001-09-27
IPC分类号： C01B1332
CPC分类号： C01B13/02 , C08F8/18 , C08F8/40 , C08F8/26 , C08F212/08 , C08F212/14 , C08F212/36
摘要： Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.
摘要翻译： 公开了改进的聚合物固定化光敏剂以及制备和使用它们的方法。 聚合物固定的光敏剂包括交联的聚合物主链，共价结合到聚合物主链上的多个阳离子铵或鏻基团和固定的光敏剂。 铵或鏻基团中的平均总碳原子数为至少四个，优选至少12.光敏剂可以共价或离子地结合到聚合物上。 本发明的聚合物负载的光敏剂在催化含有碳 - 碳双键的化合物的光敏化氧化方面出人意料地优异。
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10.发明授权Enzyme-catalyzed chemiluminescent detection of proteins and nucleic acids using hydroxyaryl cyclic diacylhydrazide compounds 失效使用羟基芳基环二酰基酰肼化合物的酶催化化学发光检测蛋白质和核酸
公开(公告)号：US5601977A
公开(公告)日：1997-02-11
申请号：US996110
申请日：1992-12-23
CPC分类号： G01N33/581 , C12Q1/28 , C12Q1/6816 , Y10S435/966 , Y10S435/968 , Y10S436/819
摘要： A method and test kit using a hydroxyaryl cyclic diacylhydrazide, a peroxide and a peroxidase enzyme on a blotting membrane for detecting DNA, RNA or proteins (polypeptides) is described. The method and kit provides enhanced chemiluminescence in an assay because of the use of the membrane. The method and test kit is particularly useful in Western, Southern and Northern blotting type assays and DNA sequencing.
摘要翻译： 描述了在用于检测DNA，RNA或蛋白质（多肽）的印迹膜上使用羟基芳基环二酰肼，过氧化物和过氧化物酶的方法和测试试剂盒。 该方法和试剂盒由于使用膜而在分析中提供增强的化学发光。 该方法和测试试剂盒在Western，Southern和Northern印迹法测定和DNA测序中特别有用。
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