公开(公告)号：US5275951A

公开(公告)日：1994-01-04

申请号：US939570

申请日：1992-09-03

申请人： Herbert S. Chow ,  John J. Kotlarik ,  Mieczyslaw Wroblewski ,  Thomas J. Wilson ,  Jimmy D. McCoy

发明人： Herbert S. Chow ,  John J. Kotlarik ,  Mieczyslaw Wroblewski ,  Thomas J. Wilson ,  Jimmy D. McCoy

IPC分类号： G01N21/17 ,  B01L3/00 ,  B01L3/02 ,  G01N15/14 ,  G01N21/82 ,  G01N33/48 ,  G01N33/483 ,  G01N33/543 ,  G01N33/80 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G02B7/28 ,  G06T1/00 ,  G01F23/00 ,  G01F23/28

CPC分类号： G01N35/109 ,  B01L3/0262 ,  B01L3/5085 ,  G01N15/1468 ,  G01N21/82 ,  G01N35/0095 ,  G01N35/0098 ,  G01N35/028 ,  G02B7/28 ,  G01N2035/042 ,  G01N2035/0425 ,  G01N2035/0491 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1034 ,  G01N2201/0446 ,  G01N2201/0461 ,  G01N2201/0492 ,  G01N35/1004 ,  Y10T137/7306 ,  Y10T436/115831

摘要： An apparatus is provided which includes a reaction cartridge having a plurality of reaction wells having different reagents disposed thereon, at least one well adapted to receive a sample, a well containing particles adapted to bind to the sample and which have the capability of being separated from cells which are not bound to the separation particles and at least one fluorophore adapted to bind to a specific type of cell in the sample, and a wash area adapted for washing a probe. An image forming device is provided to detect images which indicate whether specific reactions have occurred in each of the reaction wells. The apparatus also includes a mechanism for dispensing and aspirating liquids including a mechanism for detecting liquid levels. The device further includes logic for analyzing the information received from the image detection apparatus and processing the information to generate a visual indication of the results of the assays being performed. A microprocessor is provided to assist in the operation of the device as well as in the image processing.

摘要翻译： 提供了一种装置，其包括具有多个反应孔的反应筒，反应孔具有设置在其上的不同试剂，至少一个适于接收样品的孔，含有适于结合样品的颗粒的孔，并具有与样品分离的能力 不结合分离颗粒的细胞和至少一种适于结合样品中特定类型的细胞的荧光团和适于洗涤探针的洗涤区域。 提供图像形成装置以检测指示每个反应孔中是否发生特定反应的图像。 该装置还包括用于分配和抽吸液体的机构，包括用于检测液位的机构。 该装置还包括用于分析从图像检测装置接收的信息并处理信息以生成正在执行的测定结果的视觉指示的逻辑。 提供微处理器以帮助设备的操作以及图像处理。

公开(公告)号：US5795784A

公开(公告)日：1998-08-18

申请号：US716079

申请日：1996-09-19

申请人： David C. Arnquist ,  Grady Barnes, III ,  Chadwick M. Dunn ,  Richard C. East, Jr. ,  Patrick P. Fritchie ,  Gregory E. Gardner ,  Cass J. Grandone ,  Robert C. Gray ,  James T. Holen ,  Jimmy D. McCoy ,  James E. Mitchell ,  Adrian John Murray ,  David W. Murray ,  Jack F. Ramsey ,  Neal T. Sleszynski

发明人： David C. Arnquist ,  Grady Barnes, III ,  Chadwick M. Dunn ,  Richard C. East, Jr. ,  Patrick P. Fritchie ,  Gregory E. Gardner ,  Cass J. Grandone ,  Robert C. Gray ,  James T. Holen ,  Jimmy D. McCoy ,  James E. Mitchell ,  Adrian John Murray ,  David W. Murray ,  Jack F. Ramsey ,  Neal T. Sleszynski

IPC分类号： G01N33/483 ,  G01N33/49 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04

CPC分类号： G01N35/04 ,  G01N35/0092 ,  G01N2035/00534 ,  G01N2035/00752 ,  G01N2035/0465 ,  G01N2035/0467 ,  G01N35/025 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/115831

摘要： Embodiments described herein provide methods of performing a process for determining an item of interest in a sample. In one embodiment, a container for holding the sample is accepted in a process lane where a process step is selectively automatically performed on the sample in the container. The process step is selectively automatically performed on the sample in the container. An effective length of the process lane is maintained constant while a physical length of the process lane is selectively varied.

摘要翻译： 本文描述的实施例提供了执行用于确定样本中的感兴趣项目的过程的方法。 在一个实施例中，用于保持样品的容器在处理通道中被接受，其中对容器中的样品选择性地自动执行处理步骤。 选择性地自动对容器中的样品进行处理步骤。 过程车道的有效长度保持恒定，同时选择性地改变过程车道的物理长度。

公开(公告)号：US06562298B1

公开(公告)日：2003-05-13

申请号：US09298711

申请日：1999-04-23

申请人： David C. Arnquist ,  Grady Barnes, III ,  Richard D. Button ,  Chadwick M. Dunn ,  Richard C. East, Jr. ,  Patrick P. Fritchie ,  Charles M. Galitz ,  Gregory E. Gardner ,  Cass J. Grandone ,  Robert C. Gray ,  James T. Holen ,  Robert P. Luoma, II ,  Jimmy D. McCoy ,  James E. Mitchell ,  Adrian John Murray ,  David W. Murray ,  Jack F. Ramsey ,  Neal T. Sleszynski ,  Julius J. Toth

发明人： David C. Arnquist ,  Grady Barnes, III ,  Richard D. Button ,  Chadwick M. Dunn ,  Richard C. East, Jr. ,  Patrick P. Fritchie ,  Charles M. Galitz ,  Gregory E. Gardner ,  Cass J. Grandone ,  Robert C. Gray ,  James T. Holen ,  Robert P. Luoma, II ,  Jimmy D. McCoy ,  James E. Mitchell ,  Adrian John Murray ,  David W. Murray ,  Jack F. Ramsey ,  Neal T. Sleszynski ,  Julius J. Toth

IPC分类号： G01N3504

CPC分类号： G01N35/0092 ,  G01N35/025 ,  G01N35/04 ,  G01N2035/00534 ,  G01N2035/00752 ,  G01N2035/0465 ,  G01N2035/0467 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/115831

摘要： The embodiments disclosed relate to determination of an item of interest in a sample. One embodiment relates to a structure which comprises a process path. The process path comprises a process lane including a process step performance lane where a process step is performed, and a process step avoidance lane where the process step is avoided. A first prime mover is operatively connected with the process path for moving a container holding the sample along the process path. A first pipetting system is operatively associated with the process path for introducing the sample to the container. A second pipetting system is operatively associated with the process path for introducing a reagent to the container. A device is operatively connected with the process path and is selectively engagable with the container for mixing the sample and the reagent in the container. A second prime mover is operatively connected with the process path for selectively positioning the container in a selected one of the process step performance lane and the process step avoidance lane. A reader is operatively connected with the process path for determining the item of interest in the sample based upon a reaction between the sample and the reagent.

摘要翻译： 所公开的实施例涉及确定样品中的感兴趣物品。 一个实施例涉及包括处理路径的结构。 处理路径包括包括执行处理步骤的处理步骤执行通道的处理通道，以及避免处理步骤的处理步骤回避通道。 第一原动机与工艺路径可操作地连接，用于沿着处理路径移动容纳样品的容器。 第一移液系统与用于将样品引入容器的过程路径可操作地相关联。 第二移液系统与用于将试剂引入容器的过程路径可操作地相关联。 一种装置与工艺路径可操作地连接，并且与该容器选择性地接合，用于将样品和试剂混合在容器中。 第二原动机与工艺路径可操作地连接，用于将容器选择性地定位在所选择的一个工艺步骤性能车道和工艺步骤回避车道中。 读取器可操作地与处理路径连接，用于基于样品和试剂之间的反应来确定样品中的感兴趣物品。

公开(公告)号：US5856194A

公开(公告)日：1999-01-05

申请号：US715780

申请日：1996-09-19

申请人： David C. Arnquist ,  Grady Barnes, III ,  Richard D. Button ,  Chadwick M. Dunn ,  Richard C. East, Jr. ,  Patrick P. Fritchie ,  Charles M. Galitz ,  Gregory E. Gardner ,  Cass J. Grandone ,  Robert C. Gray ,  James T. Holen ,  Robert P. Luoma, II ,  Jimmy D. McCoy ,  James E. Mitchell ,  Adrian John Murray ,  David W. Murray ,  Jack F. Ramsey ,  Neal T. Sleszynski ,  Julius J. Toth

发明人： David C. Arnquist ,  Grady Barnes, III ,  Richard D. Button ,  Chadwick M. Dunn ,  Richard C. East, Jr. ,  Patrick P. Fritchie ,  Charles M. Galitz ,  Gregory E. Gardner ,  Cass J. Grandone ,  Robert C. Gray ,  James T. Holen ,  Robert P. Luoma, II ,  Jimmy D. McCoy ,  James E. Mitchell ,  Adrian John Murray ,  David W. Murray ,  Jack F. Ramsey ,  Neal T. Sleszynski ,  Julius J. Toth

IPC分类号： G01N33/483 ,  G01N33/49 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04

CPC分类号： G01N35/0092 ,  G01N35/04 ,  G01N2035/00534 ,  G01N2035/00752 ,  G01N2035/0465 ,  G01N2035/0467 ,  G01N35/025 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/115831

摘要： The embodiments disclosed relate to determination of an item of interest in a sample. In one method, a process path comprising a process lane including a process step performance lane where a process step is performed, and a process step avoidance lane where the process step is avoided is provided. A container holding the sample is moved along the process path. The sample is introduced to the container. A reagent is introduced to the container. The sample and the reagent are mixed in the container. The container is selectively positioned in a selected one of the process step performance lane and the process step avoidance lane. The item of interest in the sample is determined based upon a reaction between the sample and the reagent.

摘要翻译： 所公开的实施例涉及确定样品中的感兴趣物品。 在一种方法中，提供了一种处理路径，其包括包括执行处理步骤的处理步骤执行通道的处理通道，以及避免处理步骤的处理步骤回避通道。 保持样品的容器沿着处理路径移动。 将样品引入容器。 将试剂引入容器。 样品和试剂在容器中混合。 容器选择性地定位在所选择的一个工艺步骤性能车道和工艺步骤回避车道中。 基于样品和试剂之间的反应确定样品中的目标物。