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11 条记录 (耗时 0.017 秒)

    Process for obtaining cholesterol esterase from microorganisms
    1.
    发明授权
    Process for obtaining cholesterol esterase from microorganisms 失效
    从微生物获得胆固醇酯酶的方法

    公开(公告)号：US4615981A

    公开(公告)日：1986-10-07

    申请号：US663149

    申请日：1984-10-22

    申请人： Herbert von der Eltz ,  Helmgard Gauhl ,  Hans Seidel

    发明人： Herbert von der Eltz ,  Helmgard Gauhl ,  Hans Seidel

    IPC分类号： C12N9/16 ,  C12N9/18 ,  C12R1/38

    CPC分类号： C12N9/18 ,  Y10S435/874

    摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: ##STR1## in which R and R.sub.1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R.sub.1 can also be a hydrogen atom and R.sub.2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula --CH.sub.2 --(CHOH).sub.n --CH.sub.2 OH, in which n is a whole number of from 1 to 4.

    摘要翻译： 本发明提供从微生物获得胆固醇酯酶的方法，通过在电感器存在下培养能够在合适营养培养基中形成胆固醇酯酶的微生物，并从培养液和/或细胞中获得酶 其中所用的电感是通式为：其中R和R 1为含有14至18个碳原子的烷基或烷氧基的化合物，R或R ​​1也可以是氢原子，R 2是含有2个烷基氨基的化合物 至8个碳原子，含有3至8个碳原子的烷基 - 三甲基铵基团，烷基部分含有至多4个碳原子的烷基吡啶基团或通式-CH 2 - （CHOH）n -CH 2 OH基团，其中n 是从1到4的整数。

    Process for obtaining maltose phosphorylase and .beta.-phosphoglucomutase and method of determining .alpha.-amylase using same
    3.
    发明授权
    Process for obtaining maltose phosphorylase and .beta.-phosphoglucomutase and method of determining .alpha.-amylase using same 失效
    获得麦芽糖磷酸化酶和β-磷酸果糖变位酶的方法和使用其确定α-淀粉酶的方法

    公开(公告)号：US4237221A

    公开(公告)日：1980-12-02

    申请号：US953723

    申请日：1978-10-20

    申请人： Helmgard Gauhl ,  Hans Seidel

    发明人： Helmgard Gauhl ,  Hans Seidel

    IPC分类号： C12N9/10 ,  C12N9/12 ,  C12Q1/40 ,  C12R1/225 ,  C12R1/24 ,  C12R1/25 ,  C12R1/46 ,  C12Q1/32 ,  C12Q1/48

    CPC分类号： C12N9/1051 ,  C12N9/12 ,  C12Q1/40

    摘要： The present invention provides a process for obtaining maltose phosphorylase and/or .beta.-phosphoglucomutase from micro-organisms, wherein the starting material used is selected from Lactobacillus brevis DSM 20054, NCIB 8836, 8561 and 8562, Lactobacillus plantarum DSM 20174 and 43, Lactobacillus reuteri DSM 20016, Lactobacillus fermentum DSM 20052, Streptococcus spec. DSM 1118, DSM 119, DSM 1120 and DSM 1121.The present invention also provides a composition and process for determining .alpha.-amylase, wherein maltose phosphorylase and .beta.-phosphoglucomutase obtained by such a process is used, as a crude extract or in an enriched form, optionally with the addition of .alpha.-glucose-1,6-diphosphate and of divalent manganese ions.

    摘要翻译： 本发明提供从微生物获得麦芽糖磷酸化酶和/或β-磷酸果糖调蛋白酶的方法，其中使用的起始材料选自短乳杆菌DSM 20054，NCIB8836,8561和8562，植物乳杆菌DSM 20174和43，罗伊氏乳杆菌（Lactobacillus reuteri） DSM 20016，Lactobacillus fermentum DSM 20052，Streptococcus spec。 DSM 1118，DSM 119，DSM 1120和DSM 1121.本发明还提供了用于测定α-淀粉酶的组合物和方法，其中使用通过这种方法获得的麦芽糖磷酸化酶和β-磷酸果糖调蛋白酶作为粗提取物或富集 形式，任选加入α-葡萄糖-1,6-二磷酸酯和二价锰离子。

    Determination of NAD(P)H or salicylate
    6.
    发明授权
    Determination of NAD(P)H or salicylate 失效
    测定NAD（P）H或水杨酸

    公开(公告)号：US4416983A

    公开(公告)日：1983-11-22

    申请号：US328312

    申请日：1981-12-07

    申请人： Albert Roder ,  Joachim Siedel ,  Hans Mollering ,  Hans Seidel ,  Helmgard Gauhl

    发明人： Albert Roder ,  Joachim Siedel ,  Hans Mollering ,  Hans Seidel ,  Helmgard Gauhl

    IPC分类号： G01N33/66 ,  C12Q1/00 ,  C12Q1/26 ,  C12N9/02 ,  C12Q1/14 ,  C12Q1/32 ,  C12Q1/38 ,  C12Q1/40 ,  C12Q1/54 ,  C12Q1/56

    CPC分类号： C12Q1/26 ,  C12Q1/008 ,  Y10S435/805 ,  Y10S435/81

    摘要： The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

    摘要翻译： 本发明提供了测定NAD（P）H或水杨酸盐的方法，其中在NAD（P）H依赖性反应中，水杨酸盐被水杨酸羟化酶脱羧，并且着色材料由 通过与合适的着色材料组分的氧化偶联来存在酪氨酸酶，形成的有色材料然后被光度测定。 本发明还提供了用于测定NADH或NADPH的试剂，其中含有水杨酸盐，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液，以及用于测定水杨酸盐的试剂，其中它含有NAD（P ）H，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液。

    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof
    10.
    发明授权
    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
    核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途

    公开(公告)号：US4816393A

    公开(公告)日：1989-03-28

    申请号：US704712

    申请日：1985-02-25

    申请人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    发明人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    IPC分类号： C12N9/14 ,  C12N9/86 ,  C12Q1/34 ,  C12R1/01 ,  C12R1/06 ,  C12R1/13 ,  C12R1/265 ,  G01N33/70 ,  C12Q1/26 ,  C12Q1/32

    CPC分类号： C12N9/86 ,  C12Q1/34 ,  G01N33/70 ,  G01N2333/986 ,  Y10S435/81

    摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.

    摘要翻译： 本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。 本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。 此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解， 测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。