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公开(公告)号：US20100122360A1
公开(公告)日：2010-05-13
申请号：US12583559
申请日：2009-08-21
申请人： Hiromitsu Nakauchi , Toshihiro Kobayashi , Younsu Lee , Joichi Usui , Tomoyuki Yamaguchi , Sanae Hamanaka
发明人： Hiromitsu Nakauchi , Toshihiro Kobayashi , Younsu Lee , Joichi Usui , Tomoyuki Yamaguchi , Sanae Hamanaka
CPC分类号： A01K67/0271 , A01K2207/20 , A61L27/3834
摘要： An object of the present invention is to produce a mammalian organ having a complicated cellular composition composed of multiple kinds of cells, such as kidney, pancreas, thymus and hair, in the living body of a non-human animal. The inventors of the present invention applied the chimeric animal assay described above, to a novel solid organ production method. More specifically, the inventors has shown that a model mouse which is deficient of kidney, pancreas, thymus or hair due to the dysfunction of the metanephric mesenchyme that is differentiated into most of an adult kidney, is rescued by blastocyst complementation by the chimeric animal assay, and whereby a kidney, a pancreas, thymus or hair can be newly produced.
摘要翻译： 本发明的目的是在非人动物的活体中制备由多种细胞如肾，胰腺，胸腺和毛发组成的细胞组成复杂的哺乳动物器官。 本发明的发明人将上述嵌合动物实验应用于新型的固体器官生产方法。 更具体地，本发明人已经表明，由分化成大部分成年肾脏的间质间质的功能障碍引起的肾，胰腺，胸腺或毛发缺陷的模型小鼠通过嵌合动物试验通过囊胚互补来拯救 ，由此可以新生产肾，胰腺，胸腺或毛发。
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2.发明申请METHOD FOR PRODUCING FOUNDER ANIMAL FOR REPRODUCING ANIMAL HAVING LETHAL PHENOTYPE CAUSED BY GENE MODIFICATION 审中-公开生产基因动物的方法，用于复制由基因修饰引起的具有原始基因的动物公开(公告)号：US20110067125A1
公开(公告)日：2011-03-17
申请号：US12918694
申请日：2009-02-23
IPC分类号： A01K67/027 , C12N15/06
CPC分类号： A61K31/7088 , A01K67/0271 , A01K67/0275 , A01K2207/00 , A01K2217/00 , A01K2227/105 , A01K2267/03 , C12N15/8509
摘要： An object of the present invention is to provide a method which constantly enables organ regeneration for the purpose of achieving organ regeneration with higher efficiency. It has been discovered that, in a blastocyst complementation method, a next generation is born when a deficiency in an organ, such as pancreas and kidney, is complemented by injection of ES cells into a generated blastocyst, and further discovered that a transgenic animal having a pancreas or a kidney thus complemented can transmit the phenotype to the next generation as a founder. This discovery has revealed that organ regeneration can be accomplished by using such a founder. Thus, the present invention achieved the above-described object.
摘要翻译： 本发明的目的是提供一种能够以更高效率实现器官再生的目的，能够使器官再生持续的方法。 已经发现，在囊胚互补方法中，当通过将ES细胞注射到产生的囊胚中来补充器官如胰腺和肾脏的缺乏时，产生下一代，并且还发现具有 如此补充的胰腺或肾脏可以将表型传播给下一代作为创始人。 这一发现揭示了器官再生可以通过使用这样的创始人来完成。 因此，本发明实现了上述目的。
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3.发明申请METHOD FOR PRODUCING HETEROGENOUS EMBRYONIC CHIMERIC ANIMAL USING A STEM CELL 审中-公开使用干细胞生产异构胚胎动物的方法公开(公告)号：US20110283374A1
公开(公告)日：2011-11-17
申请号：US13146977
申请日：2010-01-29
申请人： Hiromitsu Nakauchi , Toshihiro Kobayashi , Tomoyuki Yamaguchi , Sanae Hamanaka , Masumi Hirabayashi , Megumi Kato
发明人： Hiromitsu Nakauchi , Toshihiro Kobayashi , Tomoyuki Yamaguchi , Sanae Hamanaka , Masumi Hirabayashi , Megumi Kato
IPC分类号： A01K67/027
CPC分类号： C12N15/8775 , A01K2267/025 , C12N15/877
摘要： A heterologous chimeric animal can be produced by a method comprising the steps of: (A) injecting a stem cell into a blastocyst cavity in a blastocyst stage of an animal heterologous to that of the stem cell, or mixing the stem cell with a divided fertilized egg of the animal heterologous to that of the stem cell; and (B) growing a cell mass including the stem cell prepared in the step (A) into a chimeric animal between a species of the stem cell and a species of the heterologous animal.
摘要翻译： 异源嵌合动物可以通过包括以下步骤的方法产生：（A）将干细胞注射到与干细胞异源的动物的胚泡期的胚泡腔中，或将干细胞与分配的受精 动物的卵与干细胞的异源; 和（B）将包含步骤（A）中制备的干细胞的细胞团生长成干细胞种和异源动物物种之间的嵌合动物。
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4.发明申请ORGAN REGENERATION METHOD UTILIZING iPS CELL AND BLASTOCYST COMPLEMENTATION 审中-公开使用iPS细胞和BLASTOCYST补充的组织再生方法公开(公告)号：US20110258715A1
公开(公告)日：2011-10-20
申请号：US13059941
申请日：2009-08-21
IPC分类号： A01K67/027 , A01K67/00 , C12N15/87
CPC分类号： A01K67/0271 , A01K67/0275 , A01K67/0276 , A01K2207/12 , A01K2227/105 , A01K2267/025 , A61L27/3834 , A61L27/3839 , A61L27/3895
摘要： It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.
摘要翻译： 通过利用囊胚互补方法将发生的多能干细胞（iPS细胞）注射到发育的囊胚中，通过利用器官的不足补充了这一事实，能够再生胰脏等器官。 因此，本发明解决了上述目的。 这提供了使用iPS细胞在发育阶段中与目标器官缺乏发育相关的异常的非人哺乳动物的活体中产生靶器官的方法，所产生的靶器官来自 作为与非人哺乳动物不同的个体的不同个体哺乳动物。
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公开(公告)号：US20050130302A1
公开(公告)日：2005-06-16
申请号：US10952460
申请日：2004-09-28
IPC分类号： A61K35/12 , A61K48/00 , C07K14/82 , C07K16/28 , C12N5/076 , C12N5/0789 , C12N5/0797 , C12N5/08
CPC分类号： C12N5/061 , A61K48/00 , A61K2035/124 , C07K14/82 , C07K16/28 , C12N5/0623 , C12N5/0647 , C12N2501/125 , C12N2501/145 , C12N2501/26 , C12N2501/998
摘要： A method and substance for regulating expansion of a stem cell, such as a hematopoietic stem cell, is provided. Therefore, a method is provided for regulating expansion of a stem cell, such as a hematopoietic stem cell, a germ line stem cell, or a neural stem cell, comprising the steps of (A) providing, to the stem cell, Bmi-1 or a variant or fragment thereof and/or a Bmi-1 regulating agent in an amount sufficient for regulation of the expansion of the stem cell and (B) culturing the stem cell for a time sufficient for the regulation of expansion.
摘要翻译： 提供了用于调节干细胞扩增的方法和物质，例如造血干细胞。 因此，提供了一种用于调节干细胞如造血干细胞，胚系干细胞或神经干细胞的扩增的方法，包括以下步骤：（A）向干细胞提供Bmi-1 或其变体或片段和/或Bmi-1调节剂，其量足以调节干细胞的扩增，和（B）培养干细胞足以调节扩张的时间。
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公开(公告)号：US07150990B2
公开(公告)日：2006-12-19
申请号：US10093311
申请日：2002-03-06
CPC分类号： C12N5/0676 , A61K35/12 , C12N5/0672 , C12N2506/02 , C12N2506/14 , Y02A50/463
摘要： The present invention provides clonal pluripotent hepatic stem cells using flow cytometry and in vitro single-cell-based assays. These cells possess multilineage differentiation potential and self-renewing capability. These cells may be clonally propagated in culture, to continuously produce hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When expanded cells are transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes, with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. Thus, the self-renewing multipotent stem cells persist in the developing mouse liver and can be induced to become cells of other organs of endodermal origin under appropriate microenvironment, providing new insight into therapies for diseases of the digestive system.
摘要翻译： 本发明使用流式细胞术和体外基于单细胞的测定法提供克隆多能肝干细胞。 这些细胞具有多谱分化潜能和自我更新能力。 这些细胞可以在培养物中克隆繁殖，以连续产生肝细胞和胆管细胞作为后代，同时保持原始干细胞。 当将扩增的细胞移植到受体动物中时，其形态和功能上分化为肝细胞和胆管细胞，重组肝细胞和胆管结构。 此外，当移植到胰腺或十二指肠壁时，这些细胞分化为胰腺导管和腺泡细胞或肠上皮细胞。 因此，自我更新的多能干细胞持续存在于发育中的小鼠肝脏中，并可在适当的微环境下诱导成为内胚层起源的其他器官的细胞，为消化系统疾病的治疗提供新的见解。
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公开(公告)号：US09200254B2
公开(公告)日：2015-12-01
申请号：US13496321
申请日：2010-09-15
申请人： Koji Eto , Naoya Takayama , Sou Nakamura , Hiromitsu Nakauchi
发明人： Koji Eto , Naoya Takayama , Sou Nakamura , Hiromitsu Nakauchi
CPC分类号： C12N5/0641 , A61K35/00 , C12N5/0644 , C12N2501/40 , C12N2501/48 , C12N2501/60 , C12N2501/606 , C12N2506/02 , C12N2506/45
摘要： The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.
摘要翻译： 本发明的目的是提供通过在期望的分化阶段中扩增细胞来产生特定细胞的方法。 本发明提供通过诱导细胞分化来产生特异性细胞的方法，其中癌细胞在期望的分化阶段的细胞中被强制表达以扩增所需分化阶段的细胞。 本发明还提供了一种制备特定细胞的方法，其中抑制了在所需分化阶段中在细胞中表达的癌基因诱导的致癌基因诱导的衰老（OIS）。
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公开(公告)号：US09034922B2
公开(公告)日：2015-05-19
申请号：US13824325
申请日：2011-09-16
申请人： Koji Eto , Ryoko Ohnishi , Hiromitsu Nakauchi , Takahiko Murata
发明人： Koji Eto , Ryoko Ohnishi , Hiromitsu Nakauchi , Takahiko Murata
IPC分类号： A61K31/27 , A01N1/02 , A61K31/165 , A61K31/18 , A61K31/4453 , A61K31/5375 , C07D295/135 , C07C317/28 , C12N5/078 , A61K39/00
CPC分类号： A01N1/0226 , A61K31/165 , A61K31/18 , A61K31/4453 , A61K31/5375 , A61K2039/55555 , C07C317/28 , C07D295/135 , C12N5/0644 , C12N2501/70 , C12N2501/734
摘要： A composition for maintaining a function of platelets, the composition comprising, as an active ingredient, a compound represented by the following general formula (I) or a salt thereof, or a solvate thereof: wherein X represents a phenylene group; Y represents any one of a hydrogen atom and —(CH2)mR1; wherein m represents an integer of any one of 0 to 4; and R1 is any one of —NR5COR2, —NR5SO2R2, and —NR3R4; wherein R2 represents any one of a C1 to C6 alkyl group, an aryl group, a C1 to C6 alkoxy group, and the like; R3 and R4 represent a C1 to C6 alkyl group or the like; and R5 represents any one of a hydrogen atom, a C1 to C6 alkyl group, and the like; and Z represents any one of a hydrogen atom and a C1 to C6 alkyl group.
摘要翻译： 用于维持血小板功能的组合物，所述组合物包含作为活性成分的由以下通式（I）表示的化合物或其盐或其溶剂合物：其中X表示亚苯基; Y表示氢原子和 - （CH 2）m R 1中的任一个; 其中m表示0至4中任一个的整数; 并且R 1是-NR 5 COR 2，-NR 5 SO 2 R 2和-NR 3 R 4中的任一个; 其中R2表示C1至C6烷基，芳基，C1至C6烷氧基等中的任一个; R3和R4表示C1〜C6烷基等; R5表示氢原子，C1〜C6烷基等中的任意一种， Z表示氢原子和C1〜C6烷基中的任一个。
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公开(公告)号：US20140127815A1
公开(公告)日：2014-05-08
申请号：US14117465
申请日：2012-05-11
申请人： Koji Eto , Hiromitsu Nakauchi , Naoya Takayama , Sou Nakamura
发明人： Koji Eto , Hiromitsu Nakauchi , Naoya Takayama , Sou Nakamura
CPC分类号： C12N15/85 , A61K35/19 , C12N5/0644 , C12N2501/165 , C12N2501/48 , C12N2506/02
摘要： An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.
摘要翻译： 本发明的目的是提供一种促进巨核细胞多倍体化，从而产生高度多倍体的巨核细胞的方法，一种从多倍体巨核细胞中有效产生血小板的方法等。 本发明提供多倍体巨核细胞的制造方法，其特征在于，在多倍体化前，在巨核细胞中强制表达凋亡抑制基因的步骤，培养所得细胞。
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公开(公告)号：US20120238023A1
公开(公告)日：2012-09-20
申请号：US13496321
申请日：2010-09-15
申请人： Koji Eto , Naoya Takayama , Sou Nakamura , Hiromitsu Nakauchi
发明人： Koji Eto , Naoya Takayama , Sou Nakamura , Hiromitsu Nakauchi
CPC分类号： C12N5/0641 , A61K35/00 , C12N5/0644 , C12N2501/40 , C12N2501/48 , C12N2501/60 , C12N2501/606 , C12N2506/02 , C12N2506/45
摘要： The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.
摘要翻译： 本发明的目的是提供通过在期望的分化阶段中扩增细胞来产生特定细胞的方法。 本发明提供通过诱导细胞分化来产生特异性细胞的方法，其中癌细胞在期望的分化阶段的细胞中被强制表达以扩增所需分化阶段的细胞。 本发明还提供了一种制备特定细胞的方法，其中抑制了在所需分化阶段中在细胞中表达的癌基因诱导的致癌基因诱导的衰老（OIS）。
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