公开(公告)号：US09890404B2

公开(公告)日：2018-02-13

申请号：US13992242

申请日：2011-12-08

申请人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

发明人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

IPC分类号： C12P13/00 ,  C12N15/77 ,  C07K14/34 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12R1/15

CPC分类号： C12P13/001 ,  C07K14/34 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/88 ,  C12R1/15 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y203/01035 ,  C12Y206/01013 ,  C12Y207/02008 ,  C12Y401/01017

摘要： The present invention relates to a putrescine-producing microorganism and a method for producing putrescine using the same. To be more specific, the present invention is directed to a microorganism given the ability to produce putrescine which is generated by blocking a biosynthetic pathway from ornithine to arginine, increasing the intracellular level of glutamate, enhancing the biosynthetic pathway of ornithine from glutamate, and introducing extracellular ornithine decarboxylase; and a method for producing putrescine by using the microorganism.

公开(公告)号：US20130344545A1

公开(公告)日：2013-12-26

申请号：US13992241

申请日：2011-12-08

申请人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

发明人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

IPC分类号： C12P13/10

CPC分类号： C12P13/10 ,  C07K14/34 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12R1/15 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y203/01035 ,  C12Y206/01013 ,  C12Y207/02008

摘要： The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.

摘要翻译： 本发明涉及具有改善的鸟氨酸生产能力的微生物，其中精氨酸形成鸟氨酸的生物合成途径被阻断，细胞内谷氨酸水平提高，鸟氨酸从谷氨酸生物合成途径增强， 鸟氨酸使用微生物。

公开(公告)号：US20140004577A1

公开(公告)日：2014-01-02

申请号：US13992242

申请日：2011-12-08

申请人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

发明人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C07K14/34 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/88 ,  C12R1/15 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y203/01035 ,  C12Y206/01013 ,  C12Y207/02008 ,  C12Y401/01017

摘要： The present invention relates to a putrescine-producing microorganism and a method for producing putrescine using the same. To be more specific, the present invention is directed to a microorganism given the ability to produce putrescine which is generated by blocking a biosynthetic pathway from ornithine to arginine, increasing the intracellular level of glutamate, enhancing the biosynthetic pathway of ornithine from glutamate, and introducing extracellular ornithine decarboxylase; and a method for producing putrescine by using the microorganism.

摘要翻译： 本发明涉及产生腐胺的微生物和使用其的腐胺的制造方法。 更具体地，本发明涉及一种微生物，其赋予通过阻断从鸟氨酸到精氨酸的生物合成途径产生腐胺的能力，增加谷氨酸的细胞内水平，增强鸟氨酸从谷氨酸的生物合成途径，并引入 细胞外鸟氨酸脱羧酶; 以及使用微生物制造腐胺的方法。

公开(公告)号：US09303277B2

公开(公告)日：2016-04-05

申请号：US13992241

申请日：2011-12-08

申请人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

发明人： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

IPC分类号： C12P13/10 ,  C07K14/34 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12

CPC分类号： C12P13/10 ,  C07K14/34 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12R1/15 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y203/01035 ,  C12Y206/01013 ,  C12Y207/02008

摘要： The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.

摘要翻译： 本发明涉及具有改善的鸟氨酸生产能力的微生物，其中精氨酸形成鸟氨酸的生物合成途径被阻断，细胞内谷氨酸水平提高，鸟氨酸从谷氨酸生物合成途径增强， 鸟氨酸使用微生物。

公开(公告)号：US09689009B2

公开(公告)日：2017-06-27

申请号：US13278106

申请日：2011-10-20

申请人： Jin Sook Chang ,  Jae Hyun Jo ,  Hyun Ae Bae ,  Byeong Cheol Song ,  Sol Kim ,  Hye Won Kim ,  Hye Won Um ,  Sung Hoo Jhon ,  Yong Uk Shin ,  Eun Bin Yang ,  Kyoung Min Lee ,  Soo An Shin

发明人： Jin Sook Chang ,  Jae Hyun Jo ,  Hyun Ae Bae ,  Byeong Cheol Song ,  Sol Kim ,  Hye Won Kim ,  Hye Won Um ,  Sung Hoo Jhon ,  Yong Uk Shin ,  Eun Bin Yang ,  Kyoung Min Lee ,  Soo An Shin

IPC分类号： C12P13/12 ,  C12P13/06 ,  C12N9/10 ,  C12N9/16 ,  C12N15/70 ,  C12N15/77

CPC分类号： C12P13/12 ,  C12N9/1085 ,  C12N9/16 ,  C12N15/70 ,  C12N15/77 ,  C12P13/06 ,  C12Y205/01065 ,  C12Y301/03003

摘要： The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and the activity of PhnC, PhnD, and PhnE is reduced, and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having these reduced and enhanced properties noted above are also provided herein.

公开(公告)号：US08455237B2

公开(公告)日：2013-06-04

申请号：US12812203

申请日：2009-01-07

申请人： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

发明人： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

IPC分类号： C12N1/20

CPC分类号： C12P13/08 ,  C12N9/1085 ,  C12N9/88

摘要： An L-threonine-producing Escherichia coli in which a promoter of a phosphoenolpyruvate carboxylase (ppc) gene on the chromosome is substituted with a promoter of a cysteine synthase (cysK) gene and a method of producing L-threonine by using the same are disclosed. The recombinant Escherichia coli may produce L-threonine in a high yield, and thus may be widely used in medical, pharmaceutical, and feed industries, particularly for an animal feed.

摘要翻译： 公开了其中染色体上的磷酸烯醇式丙酮酸羧化酶（ppc）基因的启动子被半胱氨酸合成酶（cysK）基因的启动子取代的产生L-苏氨酸的大肠杆菌以及通过使用它们产生L-苏氨酸的方法 。 重组大肠杆菌可以以高产率生产L-苏氨酸，因此可广泛用于医药，制药和饲料工业，特别是用于动物饲料。

公开(公告)号：US08486670B2

公开(公告)日：2013-07-16

申请号：US13658369

申请日：2012-10-23

申请人： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

发明人： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

IPC分类号： C12P13/08

CPC分类号： C12P13/08 ,  C12N9/1085 ,  C12N9/88

摘要： An L-threonine-producing Escherichia coli in which a promoter of a phosphoenolpyruvate carboxylase (ppc) gene on the chromosome is substituted with a promoter of a cysteine synthase (cysK) gene and a method of producing L-threonine by using the same are disclosed. The recombinant Escherichia coli may produce L-threonine in a high yield, and thus may be widely used in medical, pharmaceutical, and feed industries, particularly for an animal feed.

摘要翻译： 公开了其中染色体上的磷酸烯醇式丙酮酸羧化酶（ppc）基因的启动子被半胱氨酸合成酶（cysK）基因的启动子取代的产生L-苏氨酸的大肠杆菌以及通过使用它们产生L-苏氨酸的方法 。 重组大肠杆菌可以以高产率生产L-苏氨酸，因此可广泛用于医药，制药和饲料工业，特别是用于动物饲料。

公开(公告)号：US20110189739A1

公开(公告)日：2011-08-04

申请号：US12812203

申请日：2009-01-07

申请人： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

发明人： Kwang Ho Lee ,  Jae Yeong Ju ,  Ji Sun Lee ,  Young Bin Hwang ,  Sung Hoo Jhon ,  Eun Sung Koh ,  Chul Ha Kim ,  Soo An Shin

IPC分类号： C12P13/08 ,  C12N1/21

CPC分类号： C12P13/08 ,  C12N9/1085 ,  C12N9/88

摘要： An L-threonine-producing Escherichia coli in which a promoter of a phosphoenolpyruvate carboxylase (ppc) gene on the chromosome is substituted with a promoter of a cysteine synthase (cysK) gene and a method of producing L-threonine by using the same are disclosed. The recombinant Escherichia coli may produce L-threonine in a high yield, and thus may be widely used in medical, pharmaceutical, and feed industries, particularly for an animal feed.

摘要翻译： 公开了其中染色体上的磷酸烯醇式丙酮酸羧化酶（ppc）基因的启动子被半胱氨酸合成酶（cysK）基因的启动子取代的产生L-苏氨酸的大肠杆菌以及通过使用它们产生L-苏氨酸的方法 。 重组大肠杆菌可以以高产率生产L-苏氨酸，因此可广泛用于医药，制药和饲料工业，特别是用于动物饲料。

公开(公告)号：US08609396B2

公开(公告)日：2013-12-17

申请号：US12550099

申请日：2009-08-28

申请人： So Young Kim ,  Yong Uk Shin ,  In Kyung Heo ,  Hyun Ah Kim ,  Chang Il Seo ,  Ju Eun Kim ,  Sung Kwang Son ,  Sang Mok Lee ,  Sung Hoo Jhon ,  Han Jin Lee ,  Kwang Ho Na

发明人： So Young Kim ,  Yong Uk Shin ,  In Kyung Heo ,  Hyun Ah Kim ,  Chang Il Seo ,  Ju Eun Kim ,  Sung Kwang Son ,  Sang Mok Lee ,  Sung Hoo Jhon ,  Han Jin Lee ,  Kwang Ho Na

IPC分类号： C12N1/21 ,  C12P13/06

CPC分类号： C12N9/1205 ,  C12N9/93 ,  C12P13/06 ,  C12Y207/01033 ,  C12Y602/01001

摘要： Disclosed herein are a microorganism strain capable of producing the L-methionine precursor O-acetyl homoserine in high yield and a method of producing O-acetyl homoserine using the same. The microorganism strain is a strain of Escherichia sp. in which an acetyl-CoA synthase gene (acs) and/or a pantothenate kinase gene (coaA) encoding a pantothenate kinase gene refractory to feedback inhibition by CoA is introduced and expressed.

摘要翻译： 本文公开了能够以高产率生产L-甲硫氨酸前体O-乙酰高丝氨酸的微生物菌株和使用其的O-乙酰高丝氨酸的生产方法。 微生物菌株是大肠杆菌属的菌株。 其中引入并表达了编码由CoA反馈抑制难治的泛酸激酶基因的乙酰辅酶A合酶基因（acs）和/或泛酸激酶基因（coaA）。

公开(公告)号：US06884610B2

公开(公告)日：2005-04-26

申请号：US10603260

申请日：2003-06-24

申请人： Ghee Hong Jin ,  Sung Hoo Jhon ,  Hyun Hwan Lee ,  Hyune Mo Rho

发明人： Ghee Hong Jin ,  Sung Hoo Jhon ,  Hyun Hwan Lee ,  Hyune Mo Rho

IPC分类号： C11D3/386 ,  C12N1/21 ,  C12N9/20 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/20 ,  C11D3/38627

摘要： An alkaline lipase isolated from Vibrio metschnikovii RH530 and a polynucleotide sequence encoding the same are provided. The isolated alkaline lipase has an amino acid sequence of SEQ ID NO: 5 and the polynucleotide having a base sequence of SEQ ID NO: 4 encodes the alkaline lipase. The isolated alkaline lipase exhibits an optimal activity at a high pH level, that is, at pH 10˜11, and has very high ratio of residual enzyme activity and high compatibility with a surfactant, so that it can be suitably used as an enzyme for a laundry detergent.

摘要翻译： 提供了从弧菌弧菌RH530分离的碱性脂肪酶和编码该碱性脂肪酶的多核苷酸序列。 分离的碱性脂肪酶具有SEQ ID NO：5的氨基酸序列，并且具有SEQ ID NO：4的碱基序列的多核苷酸编码碱性脂肪酶。 分离的碱性脂肪酶在高pH值，即pH 10〜11时表现出最佳活性，并且具有非常高的残留酶活性比率和与表面活性剂的高相容性，因此其可以适合用作酶 洗衣粉。