公开(公告)号：US20200165650A1

公开(公告)日：2020-05-28

申请号：US16659475

申请日：2019-10-21

Applicant: Illumina, Inc.

Inventor： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC: C12P19/34 ,  C12Q1/683 ,  C12Q1/6869 ,  C12Q1/6816 ,  C12N15/10

Abstract: A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

公开(公告)号：US20160017396A1

公开(公告)日：2016-01-21

申请号：US14804068

申请日：2015-07-20

Applicant: ILLUMINA, INC.

Inventor： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/102 ,  C12Q1/6816 ,  C12Q1/683 ,  C12Q1/6869 ,  C12Q2521/301 ,  C12Q2522/101 ,  C12Q2563/131

Abstract: A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

Abstract translation: 一种富含靶核酸的方法，包括提供具有crRNA或其衍生物的内切核酸酶系统和Cas蛋白质或其变体。 所述crRNA或其衍生物含有与靶核酸区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物，从而富集靶核酸。

公开(公告)号：US10457969B2

公开(公告)日：2019-10-29

申请号：US14804068

申请日：2015-07-20

Applicant: Illumina, Inc.

Inventor： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6816 ,  C12Q1/683 ,  C12Q1/6869

Abstract: A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

公开(公告)号：US20180250672A1

公开(公告)日：2018-09-06

申请号：US15779459

申请日：2016-11-30

Applicant: Illumina, Inc.

Inventor： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC: B01L3/00 ,  C12Q1/6806

CPC classification number: B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N1/28 ,  G01N33/54333 ,  G01N2015/1006 ,  C12Q2535/122

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US20240299944A1

公开(公告)日：2024-09-12

申请号：US18664102

申请日：2024-05-14

Applicant: Illumina, Inc.

Inventor： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N1/28 ,  G01N15/10 ,  G01N33/543

CPC classification number: B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/54333 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N2015/1006

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US11203016B2

公开(公告)日：2021-12-21

申请号：US15779459

申请日：2016-11-30

Applicant: Illumina, Inc.

Inventor： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC: B01L3/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/543 ,  C12Q1/6869 ,  G01N15/10

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US12011719B2

公开(公告)日：2024-06-18

申请号：US17555013

申请日：2021-12-17

Applicant: Illumina, Inc.

Inventor： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC: B01L3/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/543 ,  C12Q1/6869 ,  G01N15/10

CPC classification number: B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/54333 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N2015/1006 ,  C12Q1/6869 ,  C12Q2535/122

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US20220184622A1

公开(公告)日：2022-06-16

申请号：US17555013

申请日：2021-12-17

Applicant: Illumina, Inc.

Inventor： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S. Fisher

IPC: B01L3/00 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/543

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.