DNA SEQUENCING USING MULTIPLE METAL LAYER STRUCTURE WITH DIFFERENT ORGANIC COATINGS FORMING DIFFERENT TRANSIENT BONDINGS TO DNA
    5.
    发明申请
    DNA SEQUENCING USING MULTIPLE METAL LAYER STRUCTURE WITH DIFFERENT ORGANIC COATINGS FORMING DIFFERENT TRANSIENT BONDINGS TO DNA 审中-公开
    使用多种金属层结构与不同有机涂层形成不同瞬态结合到DNA的DNA序列

    公开(公告)号:US20170059548A1

    公开(公告)日:2017-03-02

    申请号:US15349285

    申请日:2016-11-11

    摘要: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.

    摘要翻译: 纳米器件包括填充有导电流体的储存器和分离储存器的膜。 通过具有由绝缘层分隔的电极层的膜形成纳米孔。 某一电极层具有第一类型的有机涂层,并且一对电极层具有第二类型。 第一类型的有机涂层形成运动控制瞬态键合到用于运动控制的纳米孔中的一个分子,而第二种类型形成与分子的基底的不同键合位点的第一和第二瞬态键。 当对一对电极层施加电压时,由纳米孔中的基底产生隧穿电流,并且隧穿电流通过形成为待测量的第一和第二瞬态键作为用于区分基极的电流签名行进。 运动控制瞬态键比第一和第二瞬态键强。

    BASE-BY-BASE RATCHETING OF DNA/RNA IN A Y-SHAPED NANOCHANNEL
    7.
    发明申请
    BASE-BY-BASE RATCHETING OF DNA/RNA IN A Y-SHAPED NANOCHANNEL 有权
    在Y形NANOCHANNEL中的DNA / RNA的基碱基反应

    公开(公告)号:US20140174929A1

    公开(公告)日:2014-06-26

    申请号:US13724041

    申请日:2012-12-21

    IPC分类号: G01N27/447

    摘要: A mechanism is provided for ratcheting a double strand molecule. The double strand molecule is driven into a Y-channel of a membrane by a first voltage pulse. The Y-channel includes a stem and branches, and the branches are connected to the stem at a junction. The double strand molecule is slowed at the junction of the Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule. The double strand molecule is split into a first single strand and a second single strand by driving the double strand molecule into the junction of the Y-channel at a second voltage pulse.

    摘要翻译: 提供用于棘轮双链分子的机制。 双链分子通过第一电压脉冲被驱动到膜的Y通道中。 Y型通道包括杆和支杆,并且分支在连接处连接到杆。 基于第一电压脉冲的Y通道的连接处的双链分子比破坏双链分子的碱基对所需的力弱。 通过在第二电压脉冲下将双链分子驱动到Y通道的结中,将双链分子分成第一单链和第二单链。

    Electrical trapping and stretching of charged biomolecules by single electrode gating structure

    公开(公告)号:US10344324B2

    公开(公告)日:2019-07-09

    申请号:US14225677

    申请日:2014-03-26

    摘要: A technique is provided for controlling biomolecules in a nanodevice. A membrane has two reservoirs at opposing ends of the membrane. A nanochannel is formed in the membrane connecting the two reservoirs. A gate electrode is formed on the membrane such that the gate electrode extends laterally in a region of the nanochannel. A biomolecule is trapped in the nanochannel by applying a first voltage to the gate electrode. In response to trapping the biomolecule, the biomolecule is stretched in the nanochannel by applying a second voltage to the gate electrode. The biomolecule is stretched based on changing from the first voltage to the second voltage applied to the gate electrode.

    Nanospore sensor for detecting molecular interactions

    公开(公告)号:US10281428B2

    公开(公告)日:2019-05-07

    申请号:US15342541

    申请日:2016-11-03

    摘要: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged carbon nanotube to pass but to block a molecule attached to the carbon nanotube. The opening is filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged carbon nanotubes through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged carbon nanotubes passing into the opening, and to bias the electric field generator to determine a critical voltage related to a force of separation between the carbon nanotube and the molecule.

    Nanopore sensor for detecting molecular interactions
    10.
    发明授权
    Nanopore sensor for detecting molecular interactions 有权
    用于检测分子相互作用的纳米孔传感器

    公开(公告)号:US09588080B2

    公开(公告)日:2017-03-07

    申请号:US13968061

    申请日:2013-08-15

    摘要: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged carbon nanotube to pass but to block a molecule attached to the carbon nanotube. The opening is filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged carbon nanotubes through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged carbon nanotubes passing into the opening, and to bias the electric field generator to determine a critical voltage related to a force of separation between the carbon nanotube and the molecule.

    摘要翻译: 用于检测分子特征的纳米传感器包括具有开口的膜,该开口被配置为允许带电的碳纳米管通过,但是阻挡附着在碳纳米管上的分子。 开口充满电解液。 电场发生器被配置为相对于开口产生电场,以驱动带电的碳纳米管通过开口。 传感器电路耦合到电场发生器以感测由于带电的碳纳米管进入开口而导致的电流变化,并且偏置电场发生器以确定与碳纳米管和分子之间的分离力相关的临界电压。