公开(公告)号：US10393642B2

公开(公告)日：2019-08-27

申请号：US15474118

申请日：2017-03-30

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Yann A. Astier ,  Robert L. Bruce ,  Joshua T. Smith ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N15/00 ,  G03F7/20 ,  G01N15/10 ,  B01L3/00 ,  B03B5/48 ,  B81B1/00 ,  B81C1/00 ,  B82B3/00 ,  G03F7/00

Abstract: A technique related to sorting entities is provided. An inlet is configured to receive a fluid, and an outlet is configured to exit the fluid. A nanopillar array, connected to the inlet and the outlet, is configured to allow the fluid to flow from the inlet to the outlet. The nanopillar array includes nanopillars arranged to separate entities by size. The nanopillars are arranged to have a gap separating one nanopillar from another nanopillar. The gap is constructed to be in a nanoscale range.

公开(公告)号：US09835539B2

公开(公告)日：2017-12-05

申请号：US14749202

申请日：2015-06-24

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N15/10 ,  G01N27/327 ,  G01N33/487 ,  G01N15/02 ,  G01N15/00 ,  B01L3/00

CPC classification number: G01N15/1031 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2300/161 ,  G01N15/02 ,  G01N27/3278 ,  G01N33/48721 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

公开(公告)号：US09835538B2

公开(公告)日：2017-12-05

申请号：US14697269

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N15/10 ,  G01N27/327 ,  G01N33/487 ,  G01N15/02 ,  G01N15/00 ,  B01L3/00

CPC classification number: G01N15/1031 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2300/161 ,  G01N15/02 ,  G01N27/3278 ,  G01N33/48721 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

公开(公告)号：US09776184B2

公开(公告)日：2017-10-03

申请号：US15074701

申请日：2016-03-18

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N27/44791 ,  Y10T29/49002

Abstract: A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.

公开(公告)号：US09733188B2

公开(公告)日：2017-08-15

申请号：US14859608

申请日：2015-09-21

Applicant: International Business Machines Corporation

Inventor： Yann Andre Nicolas Astier ,  Ning Li ,  Devendra K. Sadana ,  Chao Wang

IPC: G01N21/64 ,  G01N21/05

CPC classification number: G01N21/6486 ,  G01N21/05 ,  G01N21/648 ,  G01N2021/058 ,  G01N2021/6482 ,  G01N2201/068 ,  G01N2201/0686

Abstract: Apparatus for enhancing on-chip fluorescence detection. For example, an apparatus comprises a microfluidic channel, an excitation signal enhancing structure formed on a first side of the microfluidic channel and a photodetector structure formed on a second side of the microfluidic channel. For example, the excitation signal enhancing structure enhances an excitation signal and the enhanced excitation signal excites one or more samples in the microfluidic channel to emit signals at a fluorescence wavelength at a higher rate.

公开(公告)号：US20170227464A1

公开(公告)日：2017-08-10

申请号：US15498569

申请日：2017-04-27

Applicant: International Business Machines Corporation

Inventor： Yann Andre Nicolas Astier ,  Ning Li ,  Devendra K. Sadana ,  Chao Wang

IPC: G01N21/64 ,  G01N21/05

CPC classification number: G01N21/645 ,  G01N21/05 ,  G01N21/648 ,  G01N21/6486 ,  G01N2021/058 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N2201/068 ,  G01N2201/0686

Abstract: Apparatus for enhancing on-chip fluorescence detection. For example, an apparatus comprises a microfluidic channel, an excitation signal enhancing structure formed on a first side of the microfluidic channel and a photodetector structure formed on a second side of the microfluidic channel. For example, the excitation signal enhancing structure enhances an excitation signal and the enhanced excitation signal excites one or more samples in the microfluidic channel to emit signals at a fluorescence wavelength at a higher rate.

公开(公告)号：US20160146778A1

公开(公告)日：2016-05-26

申请号：US14697269

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: G01N33/487 ,  G01N27/327 ,  G01N15/10

CPC classification number: G01N15/1031 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0864 ,  B01L2300/161 ,  G01N15/02 ,  G01N27/3278 ,  G01N33/48721 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

公开(公告)号：US09012329B2

公开(公告)日：2015-04-21

申请号：US13856471

申请日：2013-04-04

Applicant: International Business Machines Corporation

Inventor： Yann Astier ,  Jingwei Bai ,  Michael F. Lofaro ,  Satyavolu S. Papa Rao ,  Joshua T. Smith ,  Chao Wang

IPC: H01L21/311 ,  H01L21/306

CPC classification number: H01L21/30625 ,  B81B2201/0214 ,  B81C1/00087 ,  G01N33/48721

Abstract: A nanogap of controlled width in-between noble metals is produced using sidewall techniques and chemical-mechanical-polishing. Electrical connections are provided to enable current measurements across the nanogap for analytical purposes. The nanogap in-between noble metals may also be formed inside a Damascene trench. The nanogap in-between noble metals may also be inserted into a crossed slit nanopore framework. A noble metal layer on the side of the nanogap may have sub-layers serving the purpose of multiple simultaneous electrical measurements.

Abstract translation: 使用侧壁技术和化学机械抛光产生在贵金属之间的受控宽度的纳米隙。 提供电连接以实现跨越纳米隙的电流测量用于分析目的。 贵金属之间的纳米隙也可以形成在镶嵌槽内。 贵金属之间的纳米隙也可以插入交叉的狭缝纳米孔框架中。 在纳米隙侧面上的贵金属层可以具有用于多次同时电测量的子层。

公开(公告)号：US20150037843A1

公开(公告)日：2015-02-05

申请号：US14012665

申请日：2013-08-28

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Binquan Luan ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/11 ,  C12Q1/6806 ,  Y10T436/143333 ,  C12Q2565/131 ,  C12Q2565/631

Abstract: A mixed polynucleotide includes a first double stranded (ds) portion, a second portion including at least one single stranded (ss) portion, and a third ds portion. The second portion connects the first ds portion and the third ds portion to provide a modified polynucleotide.

Abstract translation: 混合多核苷酸包括第一双链（ds）部分，第二部分包括至少一个单链（ss）部分和第三ds部分。 第二部分连接第一个ds部分和第三个ds部分，以提供修饰的多核苷酸。

公开(公告)号：US09975147B2

公开(公告)日：2018-05-22

申请号：US14749309

申请日：2015-06-24

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: B07B1/00 ,  B07B13/04 ,  B07B1/46 ,  G01N15/00 ,  B82Y30/00 ,  G01N15/02

CPC classification number: B07B13/04 ,  B07B1/4609 ,  B82Y30/00 ,  G01N15/02 ,  G01N2015/0065 ,  G01N2015/0288 ,  Y10S977/84

Abstract: A technique relates sorting entities. The entities are introduced into a nanopillar array. The entities include a first population and a second population, and the nanopillar array includes nanopillars arranged to have a gap separating one from another. The nanopillars are ordered to have an array angle relative to a fluid flow direction. The entities are sorted through the nanopillar array by transporting the first population of the entities less than a predetermined size in a first direction and by transporting the second population of the entities at least the predetermined size in a second direction different from the first direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the entities having a sub-100 nanometer size.