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1.发明授权Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus 失效衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶公开(公告)号：US06255081B1
公开(公告)日：2001-07-03
申请号：US09175973
申请日：1998-10-21
IPC分类号： C12P1934
CPC分类号： C12N9/22
摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译： 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质 由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。 根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
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2.发明授权Thermostable flap endonuclease derived from a hyperthermophile bacterium belonging to the genus pyrococcus 失效衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶公开(公告)号：US06251649B1
公开(公告)日：2001-06-26
申请号：US09146319
申请日：1998-09-03
IPC分类号： C12N922
CPC分类号： C07K14/195 , C12N9/22
摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译： 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质 由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。 根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
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公开(公告)号：US07846704B2
公开(公告)日：2010-12-07
申请号：US11658096
申请日：2005-01-21
申请人： Eriko Matsui , Ikuo Matsui
发明人： Eriko Matsui , Ikuo Matsui
CPC分类号： C12N9/22
摘要： A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.
摘要翻译： 将突变引入皮瓣内切核酸酶的底物结合位点以制备具有修饰的底物特异性的突变体。 使用突变体作为遗传多态性分析的试剂，与常规方法相比，遗传多态性的分析可以更准确，更容易和灵敏地进行。
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公开(公告)号：US20080118922A1
公开(公告)日：2008-05-22
申请号：US11658096
申请日：2005-01-21
申请人： Eriko Matsui , Ikuo Matsui
发明人： Eriko Matsui , Ikuo Matsui
CPC分类号： C12N9/22
摘要： A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.
摘要翻译： 将突变引入皮瓣内切核酸酶的底物结合位点以制备具有修饰的底物特异性的突变体。 使用突变体作为遗传多态性分析的试剂，与常规方法相比，遗传多态性的分析可以更准确，更容易和灵敏地进行。
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公开(公告)号：US08736824B2
公开(公告)日：2014-05-27
申请号：US13561820
申请日：2012-07-30
申请人： Eriko Matsui , Suguru Dowaki , Hirokazu Tatsuta , Takeshi Kunihiro , Akira Niwa , Megumu Saito , Tatsutoshi Nakahata
发明人： Eriko Matsui , Suguru Dowaki , Hirokazu Tatsuta , Takeshi Kunihiro , Akira Niwa , Megumu Saito , Tatsutoshi Nakahata
CPC分类号： G01N15/05 , G01N21/31 , G01N21/33 , G01N33/49 , G01N2021/3144 , G01N2021/3155
摘要： A blood analysis apparatus includes an analysis section. The analysis section is configured to compare a measurement spectrum obtained regarding red blood cells with a standard spectrum of heme, biliverdin, and bilirubin, and cause the measurement spectrum to belong to any one of the standard spectra.
摘要翻译： 血液分析装置包括分析部。 分析部将比较红细胞的测定光谱与血红素，胆红素，胆红素的标准光谱进行比较，使测定光谱属于任一标准光谱。
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公开(公告)号：US08582823B2
公开(公告)日：2013-11-12
申请号：US13269239
申请日：2011-10-07
IPC分类号： G06K9/00
CPC分类号： G06T7/0016 , G06T7/223 , G06T2207/10016 , G06T2207/20021 , G06T2207/30024
摘要： An image processing apparatus includes: a motion detection unit detecting a motion of a subject to be evaluated by using an image of the subject to be evaluated; a correlation calculation unit calculating a temporal change correlation between motion amounts of a plurality of portions of the subject to be evaluated, by using a motion vector indicating the motion of the subject to be evaluated, which is detected by the motion detection unit; and an evaluation value calculation unit calculating an evaluation value to evaluate cooperativity of the motion of the subject to be evaluated, by using the correlation calculated by the correlation calculation unit.
摘要翻译： 一种图像处理装置，包括：运动检测单元，通过使用待评估对象的图像来检测待评估对象的运动; 相关计算单元，通过使用由运动检测单元检测到的指示被评估对象的运动的运动矢量来计算待评估对象的多个部分的运动量之间的时间变化相关性; 以及评估值计算单元，通过使用由相关计算单元计算的相关性来计算评估值，以评估待评估对象的运动的协调性。
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7.发明申请RESISTIVE ELEMENT, NEURON ELEMENT, AND NEURAL NETWORK INFORMATION PROCESSING DEVICE 审中-公开电阻元件，神经元元件和神经网络信息处理器件公开(公告)号：US20100217735A1
公开(公告)日：2010-08-26
申请号：US12664593
申请日：2008-05-27
申请人： Hajime Matsumura , Eriko Matsui
发明人： Hajime Matsumura , Eriko Matsui
CPC分类号： G06N3/002 , B82Y10/00 , G06N3/063 , H01C7/006 , H01C17/06586 , H01L51/0591
摘要： A neuron element is provided having an input part that receives a plurality of input signals and, in response to the respective input signals, creates weighted signals as the products of the input signals and connection weights corresponding to the input signals, an addition part that obtains the total sum of the plurality of weighted signals, and an output part that outputs an output signal as a function of the total sum, a functional molecular element having a current-voltage characteristic in which the current flowing through the element is represented as a function having an inflection point with respect to the applied voltage is used as an element that defines the output signal, and the output signal is defined as a function of the total sum based on the current-voltage characteristic of this element in the region including the inflection point. Thereby, a neuron element that includes an output part formed of a simple circuit and has an analogous output characteristic, and a neural network information processing device employing it are provided.
摘要翻译： 提供神经元元件，其具有接收多个输入信号的输入部分，并且响应于相应的输入信号，产生作为与输入信号相对应的输入信号和连接权重的乘积的加权信号，获得 多个加权信号的总和以及输出作为总和的函数的输出信号的输出部分，具有电流 - 电压特性的功能分子元件，其中流过元件的电流被表示为函数 使用具有相对于所施加的电压的拐点作为限定输出信号的元件，并且输出信号被定义为基于包括拐点的区域中该元件的电流 - 电压特性的总和的函数 点。 因此，提供了包括由简单电路形成并具有类似输出特性的输出部分和使用它的神经网络信息处理装置的神经元元件。
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公开(公告)号：US07642541B2
公开(公告)日：2010-01-05
申请号：US11538323
申请日：2006-10-03
申请人： Eriko Matsui , William Ford , Jurina Wessels , Akio Yasuda , Ryuichiro Maruyama , Tsuyonobu Hatazawa
发明人： Eriko Matsui , William Ford , Jurina Wessels , Akio Yasuda , Ryuichiro Maruyama , Tsuyonobu Hatazawa
IPC分类号： H01L49/00
CPC分类号： H01L51/0048 , B82Y10/00 , H01L51/0047 , H01L51/0049 , H01L51/0512 , H01L51/0595 , H01L51/105 , Y10S977/762 , Y10S977/938
摘要： A functional device which is composed of a nanometer-sized functional structure, which can reduce connection resistance in connecting the functional structure to an external electrode, and which includes a wiring section capable of minimizing constraints given to structural designs of various functional structures, and a method of manufacturing it are provided. A functional device in which a functional structure having contained sections in positions spaced from each other is retained by a carbon nanotube. A gap is formed in the carbon nanotube, and the carbon nanotube is segmented into a first carbon nanotube and a second carbon nanotube by the gap. One of the contained sections is contained in the first carbon nanotube at an opening of the first carbon nanotube facing the gap, and the other of the contained sections is contained in the second carbon nanotube at an opening of the second carbon nanotube facing the gap.
摘要翻译： 一种由纳米尺寸的功能结构构成的功能元件，其能够降低将功能结构与外部电极连接的连接电阻，并且具有能够使对各种功能结构的结构设计的约束最小化的布线部，以及 提供制造方法。 其中具有彼此间隔的位置中包含部分的功能结构的功能装置由碳纳米管保持。 在碳纳米管中形成间隙，通过间隙将碳纳米管分割成第一碳纳米管和第二碳纳米管。 包含的部分之一包含在第一碳纳米管的面向间隙的开口处的第一碳纳米管中，并且另一个包含的部分被包含在第二碳纳米管的面向间隙的第二碳纳米管的开口处。
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公开(公告)号：US6072455A
公开(公告)日：2000-06-06
申请号：US923403
申请日：1997-09-06
申请人： Eriko Matsui , Nobue Kataoka , Akio Yasuda
发明人： Eriko Matsui , Nobue Kataoka , Akio Yasuda
IPC分类号： G02F1/137 , G02F1/133 , G02F1/1333 , G02F1/141 , G09G3/36
CPC分类号： G09G3/3629 , G09G2310/061 , G09G2310/065 , G09G2320/0209
摘要： A driving method for a liquid crystal device includes the steps of providing a first substrate with a unidirectionally aligned data electrode group and a second substrate with a select-electrode group aligned perpendicularly to the data electrode; applying a select pulse to the select electrodes; and providing a pause corresponding to at least one line before a data-pulse sequence is applied to the data-electrode group. The select pulse can be synchronized with the data-pulse sequence by shifting them a half line relative to each other so that the select pulse and the data pulse have opposite polarity in relation to each other. Alternatively, the select pulse is applied while the data pulse sequence is applied to the data electrode group. In the latter case, the time and/or voltage is determined so as to offset the effects of the reversed electric field generated during switching of the liquid crystal.
摘要翻译： 液晶装置的驱动方法包括以下步骤：提供具有单向排列数据电极组的第一衬底和具有与数据电极垂直排列的选择电极组的第二衬底; 对选择电极施加选择脉冲; 并且在将数据脉冲序列应用于数据电极组之前提供对应于至少一行的暂停。 选择脉冲可以与数据脉冲序列同步，通过将它们相对于彼此移动一半，使得选择脉冲和数据脉冲相对于彼此具有相反的极性。 或者，当将数据脉冲序列施加到数据电极组时施加选择脉冲。 在后一种情况下，确定时间和/或电压以抵消在液晶切换期间产生的反向电场的影响。
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公开(公告)号：US10311581B2
公开(公告)日：2019-06-04
申请号：US13984672
申请日：2012-02-28
摘要： Apparatus and methods for display control and image processing that enable easy and noninvasive observation of an object are described. Display control of a cell image may include generating a motion amount for subregions of the cell image and displaying the motion amount superimposed on the cell image. Image processing of a cell image may include generating a motion amount for subregions of a cell image and evaluating a state of propagation of the motion of the cells.
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