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公开(公告)号：US08736824B2
公开(公告)日：2014-05-27
申请号：US13561820
申请日：2012-07-30
申请人： Eriko Matsui , Suguru Dowaki , Hirokazu Tatsuta , Takeshi Kunihiro , Akira Niwa , Megumu Saito , Tatsutoshi Nakahata
发明人： Eriko Matsui , Suguru Dowaki , Hirokazu Tatsuta , Takeshi Kunihiro , Akira Niwa , Megumu Saito , Tatsutoshi Nakahata
CPC分类号： G01N15/05 , G01N21/31 , G01N21/33 , G01N33/49 , G01N2021/3144 , G01N2021/3155
摘要： A blood analysis apparatus includes an analysis section. The analysis section is configured to compare a measurement spectrum obtained regarding red blood cells with a standard spectrum of heme, biliverdin, and bilirubin, and cause the measurement spectrum to belong to any one of the standard spectra.
摘要翻译： 血液分析装置包括分析部。 分析部将比较红细胞的测定光谱与血红素，胆红素，胆红素的标准光谱进行比较，使测定光谱属于任一标准光谱。
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公开(公告)号：US20130038860A1
公开(公告)日：2013-02-14
申请号：US13561820
申请日：2012-07-30
申请人： Eriko Matsui , Suguru Dowaki , Hirokazu Tatsuta , Takeshi Kunihiro , Akira Niwa , Megumu Saito , Tatsutoshi Nakahata
发明人： Eriko Matsui , Suguru Dowaki , Hirokazu Tatsuta , Takeshi Kunihiro , Akira Niwa , Megumu Saito , Tatsutoshi Nakahata
IPC分类号： G01N33/49
CPC分类号： G01N15/05 , G01N21/31 , G01N21/33 , G01N33/49 , G01N2021/3144 , G01N2021/3155
摘要： A blood analysis apparatus includes an analysis section. The analysis section is configured to compare a measurement spectrum obtained regarding red blood cells with a standard spectrum of heme, biliverdin, and bilirubin, and cause the measurement spectrum to belong to any one of the standard spectra.
摘要翻译： 血液分析装置包括分析部。 分析部将比较红细胞的测定光谱与血红素，胆红素，胆红素的标准光谱进行比较，使测定光谱属于任一标准光谱。
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公开(公告)号：US09499789B2
公开(公告)日：2016-11-22
申请号：US14001004
申请日：2012-02-23
IPC分类号： C12N5/0784
CPC分类号： C12N5/0639 , C12N2500/25 , C12N2500/90 , C12N2501/145 , C12N2501/155 , C12N2501/22 , C12N2501/26 , C12N2506/02 , C12N2506/45
摘要： A dendritic cell is produced from pluripotent stem cells by culturing pluripotent stem cells by the following steps: (1) performing adherent culture in a medium which comprises a BMP family protein but does not comprise serum; (2) performing adherent culture in a medium which comprises VEGF but does not comprise serum; (3) performing adherent culture in a medium which comprises a hematopoietic factor but does not comprise serum; and (4) performing suspension culture in a medium which does not comprise serum.
摘要翻译： 通过以下步骤培养多能干细胞从多能干细胞产生树突状细胞：（1）在包含BMP家族蛋白但不包含血清的培养基中进行贴壁培养; （2）在包含VEGF但不包含血清的培养基中进行贴壁培养; （3）在包含造血因子但不包含血清的培养基中进行贴壁培养; 和（4）在不含血清的培养基中进行悬浮培养。
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公开(公告)号：US20130330822A1
公开(公告)日：2013-12-12
申请号：US14001004
申请日：2012-02-23
IPC分类号： C12N5/0784
CPC分类号： C12N5/0639 , C12N2500/25 , C12N2500/90 , C12N2501/145 , C12N2501/155 , C12N2501/22 , C12N2501/26 , C12N2506/02 , C12N2506/45
摘要： A dendritic cell is produced from pluripotent stem cells by culturing pluripotent stem cells by the following steps: (1) performing adherent culture in a medium which comprises a BMP family protein but does not comprise serum; (2) performing adherent culture in a medium which comprises VEGF but does not comprise serum; (3) performing adherent culture in a medium which comprises a hematopoietic factor but does not comprise serum; and (4) performing suspension culture in a medium which does not comprise serum.
摘要翻译： 通过以下步骤培养多能干细胞从多能干细胞产生树突状细胞：（1）在包含BMP家族蛋白但不包含血清的培养基中进行贴壁培养; （2）在包含VEGF但不包含血清的培养基中进行贴壁培养; （3）在包含造血因子但不包含血清的培养基中进行贴壁培养; 和（4）在不含血清的培养基中进行悬浮培养。
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公开(公告)号：US09557321B2
公开(公告)日：2017-01-31
申请号：US13885950
申请日：2011-11-17
CPC分类号： G01N33/5055 , C12N5/0696 , G01N33/6869 , G01N33/6872 , G01N2333/545 , G01N2500/10
摘要： The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene.
摘要翻译： 本发明提供了使用衍生自具有突变体NLRP3基因的诱导多能干细胞（iPS细胞）的巨噬细胞来筛选用于抑制炎症性活性的药物的方法。
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公开(公告)号：US20130273588A1
公开(公告)日：2013-10-17
申请号：US13885950
申请日：2011-11-17
IPC分类号： G01N33/50
CPC分类号： G01N33/5055 , C12N5/0696 , G01N33/6869 , G01N33/6872 , G01N2333/545 , G01N2500/10
摘要： The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene.
摘要翻译： 本发明提供了使用衍生自具有突变体NLRP3基因的诱导多能干细胞（iPS细胞）的巨噬细胞来筛选用于抑制炎症性活性的药物的方法。
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7.发明申请公开(公告)号：US20130011924A1
公开(公告)日：2013-01-10
申请号：US13635679
申请日：2011-03-18
申请人： Akira Niwa , Tatsutoshi Nakahata , Toshio Heike
发明人： Akira Niwa , Tatsutoshi Nakahata , Toshio Heike
IPC分类号： C12N5/078 , C12N5/0787 , C12N5/077 , C12N5/071 , C12N5/0789
CPC分类号： C12N5/0647 , C12N2501/125 , C12N2501/155 , C12N2501/165 , C12N2501/415 , C12N2506/02
摘要： The present invention provides a method for inducing differentiation of pluripotent stem cells into mesodermal cells, comprising the step of culturing pluripotent stem cells in a serum-free medium without forming an embryoid body and without coculturing with cells from a different species.
摘要翻译： 本发明提供了诱导多能干细胞分化为中胚层细胞的方法，包括在无血清培养基中培养多能干细胞而不形成胚状体并且不与来自不同物种的细胞共同培养的步骤。
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8.发明授权Method for producing mesodermal cells by culturing under adherent conditions and without co-culture with cells from a different species in a serum-free medium 有权通过在粘附条件下培养而不与无血清培养基中的不同物种的细胞共培养来生产中胚层细胞的方法公开(公告)号：US08652845B2
公开(公告)日：2014-02-18
申请号：US13635679
申请日：2011-03-18
申请人： Akira Niwa , Tatsutoshi Nakahata , Toshio Heike
发明人： Akira Niwa , Tatsutoshi Nakahata , Toshio Heike
IPC分类号： C12N5/095
CPC分类号： C12N5/0647 , C12N2501/125 , C12N2501/155 , C12N2501/165 , C12N2501/415 , C12N2506/02
摘要： The present invention provides a method for inducing differentiation of pluripotent stem cells into mesodermal cells, comprising the step of culturing pluripotent stem cells in a serum-free medium without forming an embryoid body and without coculturing with cells from a different species.
摘要翻译： 本发明提供了一种诱导多能干细胞分化为中胚层细胞的方法，包括在无血清培养基中培养多能干细胞而不形成胚状体并且不与来自不同物种的细胞共同培养的步骤。
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9.发明申请公开(公告)号：US20150104820A1
公开(公告)日：2015-04-16
申请号：US13990991
申请日：2011-12-02
IPC分类号： C12N5/0787 , G01N33/50
CPC分类号： C12N5/0642 , C12N5/0696 , C12N2501/125 , C12N2501/145 , C12N2501/165 , C12N2501/20 , C12N2501/22 , C12N2501/2303 , C12N2501/2305 , C12N2501/2306 , C12N2501/26 , C12N2502/02 , C12N2506/02 , C12N2506/45 , G01N33/5029 , G01N33/5047 , G01N33/505 , G01N2500/04 , G01N2500/10
摘要： The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.
摘要翻译： 本发明涉及从人多能干细胞生产人嗜酸性粒细胞的方法。 更具体地说，本发明提供从人多能干细胞生产人嗜酸性粒细胞的方法，该方法包括以下步骤：（1）在VEGF的存在下，将人多能干细胞与从AGM区域分离的细胞共培养 的哺乳动物胎儿; （2）使用包含IL-3，IL-6，Flt3配体，SCF，TPO和血清的培养基进行悬浮培养; （3）使用包含IL-3，SCF，GM-CSF和血清的培养基进行悬浮培养; 和（4）使用包含IL-3，IL-5和血清的培养基进行悬浮培养。
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10.发明授权Hematopoietic stem cell growth-promoting compositions containing a fibroblast-derived fragment of fibronectin and a growth factor, and methods employing them in vitro or in vivo 失效含有成纤维细胞的纤连蛋白片段和生长因子的造血干细胞生长促进组合物，以及在体外或体内使用它们的方法
公开(公告)号：US5668104A
公开(公告)日：1997-09-16
申请号：US142449
申请日：1994-04-26
IPC分类号： A61K38/00 , C07K14/475 , C07K14/47 , A61K38/17 , A61K38/39
CPC分类号： C07K14/475 , A61K38/00
摘要： An 18 kDa protein, denominated FDF-3, has been isolated from cultures of human fibroblasts and has been found to potentiate the growth- and differentiation-promoting activities of certain cytokines toward hematopoietic progenitor cells. The amino acid sequence of the FDF-3 protein, shown in SEQ ID NO: 14, corresponds to the sequence of a C-terminal domain of human fibronectin. The protein is useful in combination with IL-3, G-CSF, GM-CSF, or SCF (stem cell factor) in the expansion of hematopoietic cell populations in vitro or in therapeutic methods to promote recovery from leukopenia or myelosuppresion in vivo.
摘要翻译： PCT No.PCT / JP93 / 00404 Sec。 371日期1994年04月26日 102（e）1994年4月26日PCT 1993年3月31日PCT公布。 出版物WO93 / 20228 日期1993年10月14日已经从人成纤维细胞的培养物中分离出18kDa蛋白，称为FDF-3，并且已经发现某些细胞因子对造血祖细胞的生长和分化促进活性的增强。 SEQ ID NO：14所示的FDF-3蛋白的氨基酸序列对应于人纤连蛋白的C末端结构域的序列。 在体外造血细胞群体的扩增或治疗方法中，该蛋白质可与IL-3，G-CSF，GM-CSF或SCF（干细胞因子）结合使用以促进体内从白细胞减少或骨髓间质减少的恢复。
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