摘要:
The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译:本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的DNA,或(b)具有瓣内切核酸酶活性的蛋白质 由氨基酸序列(a),一个或多个氨基酸缺失,取代或添加的氨基酸序列组成。 根据本发明,提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外,该酶是热稳定的,因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
摘要:
The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译:本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的DNA,或(b)具有瓣内切核酸酶活性的蛋白质 由氨基酸序列(a),一个或多个氨基酸缺失,取代或添加的氨基酸序列组成。 根据本发明,提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外,该酶是热稳定的,因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
摘要:
Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.
摘要:
The invention relates to a thermophilic enzyme having β-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.
摘要翻译:本发明涉及具有β-糖苷酶活性的嗜热酶,其包含SEQ ID NO:2的氨基酸序列,其中可以缺失,替换或添加一个或多个氨基酸残基。
摘要:
A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.
摘要:
An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.
摘要:
The reaction of a lipase upon a fatty acid is effected by an improved method which comprises bringing the lipase bonded at multiple points to an anion-exchange residue or a carrier and a carrier having a free anion-exchange group admixed therewith into contact with a reaction mixture containing an oily substance including the fatty acid and a water-soluble substance thereby forming an oily product and a water-soluble product in the reaction mixture and subsequently separating the two reaction products.
摘要:
An engine control device and method that prevents fuel injection quantity from becoming unstable due to a rapid change of a correction quantity even in a predetermined operation state such as acceleration time and thus stabilizes an air-fuel ratio. Under a predetermined operating state, even when feedback control of oxygen in exhaust gas is stopped, a correction quantity immediately before the predetermined operating state is held by a correction quantity storage part and the correction of the fuel injection time is performed based on the correction quantity.
摘要:
A novel olefin production process for forming at least one olefin from two or more olefins in the presence of a catalyst containing at least one selected from the group consisting of nickel, aluminum, manganese, iron and copper which is supported by a regular mesoporous material. In the above olefin production process, propylene can be formed from ethylene and butene. In the above olefin production process, water vapor can be contained in a reaction gas in a molar ratio based on the total of the raw material olefins of 0.001 to 1.
摘要:
A water-soluble substrate and an oily substrate are continuously reacted with immobilized lipase in a reaction vessel having vertically maintained apart upper and lower conically-shaped regions, respectively, for separation of a water-soluble product and an oily product, a plurality of lipase reaction zones each containing immobilized lipase capable of being fluidized and an agitating means, and a plurality of intermediate separation zones for separation of an oily substance and a water-soluble substance. The lipase reaction zones and the intermediate separation zones are disposed alternately between the upper and lower conically-shaped separation regions. Boundaries between the lipase reaction zones and intermediate separation zones are pervious to liquid but impervious to the immobilized lipase. The water-soluble substrate and oily substrate are passed in counterflow contact through the lipase reaction zones and intermediate separation zones and mutually contact the immobilized lipase which has been fluidized. An oily product is recovered from the upper conically-shaped separation region and a water-soluble product is recovered from the lower conically-shaped separation region.