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104 条记录 (耗时 0.025 秒)

    Dioxetane-precursor-labeled probes and detection assays employing the same
    1.
    发明授权
    Dioxetane-precursor-labeled probes and detection assays employing the same 失效
    二氧杂环丁烷 - 前体标记的探针和使用其的检测测定

    公开(公告)号：US5800999A

    公开(公告)日：1998-09-01

    申请号：US767479

    申请日：1996-12-16

    申请人： Irena Bronstein ,  Brooks Edwards ,  Christopher Martin ,  John Voyta

    发明人： Irena Bronstein ,  Brooks Edwards ,  Christopher Martin ,  John Voyta

    IPC分类号： G01N33/532 ,  C07D321/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  C12N15/11

    CPC分类号： C07H21/00 ,  C07D321/00 ,  C12Q1/6816 ,  C12Q1/686 ,  Y02P20/55

    摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.

    摘要翻译： 用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。 探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。 探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。 通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。 这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。 触发可能是pH温度的变化，或者是除去保护基团的试剂。 这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

    Dioxetane labeled probes and detection assays employing the same
    3.
    发明授权
    Dioxetane labeled probes and detection assays employing the same 有权
    二氧杂环丁烷标记的探针和使用其的检测测定

    公开(公告)号：US06451531B1

    公开(公告)日：2002-09-17

    申请号：US09340726

    申请日：1999-06-29

    申请人： Irena Bronstein ,  Brooks Edwards ,  Christopher Martin ,  John Voyta

    发明人： Irena Bronstein ,  Brooks Edwards ,  Christopher Martin ,  John Voyta

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  C07D321/00 ,  C12Q1/6816 ,  C12Q1/686 ,  Y02P20/55 ,  C12Q2561/101 ,  C12Q2565/113

    摘要： Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.

    摘要翻译： 用1,2-二氧杂环丁烷前体标记的探针可用于多种测定。 探针可以是核酸，肽核酸，蛋白质，包括酶，抗体或抗原，类固醇，碳水化合物，药物或非药物半抗原。 探针具有与其结合的1,2-二氧杂环丁烷前体，通常共价或强配体键。 通过暴露于单线态氧将二氧杂环丁烷前体部分转化为结合的1,2-二氧环乙烷。 这些二氧杂环丁烷（标记）会自发分解，或被适当的触发剂诱导分解以释放光。 触发可能是pH温度的变化，或者是除去保护基团的试剂。 这些1,2-二氧杂环丁烷标记的探针和指示物可用于包括杂交测定，免疫测定，基于凝胶的测定和毛细管区域电泳的测定形式。

    Multiple enzyme assays
    5.
    发明授权
    Multiple enzyme assays 有权
    多重酶测定

    公开(公告)号：US06586196B1

    公开(公告)日：2003-07-01

    申请号：US09459982

    申请日：1999-12-14

    申请人： Irena Bronstein ,  Christopher Martin ,  Corinne Olesen ,  John Voyta ,  Yu-xin Yan

    发明人： Irena Bronstein ,  Christopher Martin ,  Corinne Olesen ,  John Voyta ,  Yu-xin Yan

    IPC分类号： C12Q142

    CPC分类号： C12Q1/6897 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/66 ,  G01N2333/916 ,  G01N2333/924 ,  G01N2333/95

    摘要： The present invention discloses multiple enzyme assays which measure the activity of at least one endogenous enzyme in a single aliquot and a method of measuring the activity of multiple enzymes in an aliquot of a cell extract, wherein at least one of the enzymes is an endogenous enzyme. In one embodiment of the invention the activity of a first enzyme is quantified by measuring the light signal produced by degradation of a first enzyme substrate by the first enzyme and the activity of the second enzyme is quantified by measuring the light signal produced by the degradation of a second substrate. In the method of the present invention, both quantifications are performed on the same aliquot of cell extract. Different embodiments of the present invention provide for the detection of more than one endogenous enzyme and for the detection of at least one reporter enzyme and at least one endogenous enzyme. The present invention also discloses kits for detecting the activity of multiple enzymes.

    摘要翻译： 本发明公开了测量单一等分试样中至少一种内源性酶的活性的多种酶测定法和测量细胞提取物等分试样中多种酶的活性的方法，其中至少一种酶是内源性酶 。 在本发明的一个实施方案中，通过测量由第一酶降解第一酶底物产生的光信号来定量第一酶的活性，并且通过测量由第一酶降解产生的光信号来量化第二酶的活性 第二基板。 在本发明的方法中，两种定量在相同等分的细胞提取物上进行。 本发明的不同实施方案提供了多于一种内源性酶的检测和用于检测至少一种报道酶和至少一种内源性酶。 本发明还公开了用于检测多种酶的活性的试剂盒。

    Closure device
    9.
    发明授权
    Closure device 有权

    公开(公告)号：US09610070B2

    公开(公告)日：2017-04-04

    申请号：US12139926

    申请日：2008-06-16

    申请人： Christopher Martin

    发明人： Christopher Martin

    IPC分类号： A61B17/08 ,  A61B17/00 ,  A61B17/04

    CPC分类号： A61B17/0057 ,  A61B17/0469

    摘要： An interventional medical closure device comprises a first engagement element for engagement with an external surface of a vessel on a first side of an opening through the vessel wall and a second engagement element for engagement with an external surface on a second side of the opening. A closure element extends from the first engagement element, into the vessel, and to the second engagement element to assist in closure of the opening. The device may include a foot element to engage the internal surface of the vessel on both sides of the opening. The foot element may provide a guide with a pathway for the closure element.

    Method and apparatus for non-destructive evaluation of materials
    10.
    发明授权
    Method and apparatus for non-destructive evaluation of materials 有权
    材料无损评估的方法和装置

    公开(公告)号：US08928316B2

    公开(公告)日：2015-01-06

    申请号：US13297933

    申请日：2011-11-16

    申请人： Neil J. Goldfine ,  Andrew P. Washabaugh ,  Robert Lyons ,  Zachary Thomas ,  David A. Jablonski ,  Christopher Martin

    发明人： Neil J. Goldfine ,  Andrew P. Washabaugh ,  Robert Lyons ,  Zachary Thomas ,  David A. Jablonski ,  Christopher Martin

    IPC分类号： G01R33/12 ,  G01N27/82 ,  G01R33/10 ,  G01R33/00

    CPC分类号： G01N27/90 ,  G01N27/72 ,  G01N27/9046 ,  G01R33/0064 ,  G01R33/0094 ,  G01R33/093 ,  G01R33/10

    摘要： Methods and apparatus for characterizing composite materials for manufacturing quality assurance (QA), periodic inspection during the useful life, or for forensic analysis/material testing. System are provided that relate eddy-current sensor responses to the fiber layup of a composite structure, the presence of impact damage on a composite structure with or without a metal liner, volumetric stress within the composite, fiber tow density, and other NDE inspection requirements. Also provided are systems that determine electromagnetic material properties and material dimensions of composite materials from capacitive sensor inspection measurements. These properties are related to the presence of buried defects in non-conductive composite materials, moisture ingress, aging of the material due to service or environmental/thermal exposure, or changes in manufacturing quality.

    摘要翻译： 表征用于制造质量保证（QA）的复合材料，使用寿命期间的定期检查或法医分析/材料测试的方法和装置。 提供了将涡电流传感器响应与复合结构的纤维叠层相关联的系统，在具有或不具有金属衬垫的复合结构上的冲击损伤的存在，复合材料内的体积应力，纤维丝束密度和其他NDE检查要求 。 还提供了从电容传感器检查测量确定复合材料的电磁材料性质和材料尺寸的系统。 这些性质与非导电复合材料中存在的掩埋缺陷有关，水分进入，由于使用或环境/热暴露引起的材料老化或制造质量的变化。