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    Methods of Reprogramming Animal Somatic Cells
    5.
    发明申请
    Methods of Reprogramming Animal Somatic Cells 审中-公开
    重新编程动物体细胞的方法

    公开(公告)号：US20100167404A1

    公开(公告)日：2010-07-01

    申请号：US11989988

    申请日：2006-08-03

    申请人： Michael D. West ,  Karen B. Chapman ,  Roy Geoffrey Sargent

    发明人： Michael D. West ,  Karen B. Chapman ,  Roy Geoffrey Sargent

    IPC分类号： C12N15/00 ,  C12N5/10

    CPC分类号： C12N5/0606 ,  A01K67/0271 ,  C12N5/0676 ,  C12N5/16 ,  C12N15/873 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/72 ,  C12N2506/00 ,  C12N2506/11 ,  C12Q1/6881 ,  C12Q2600/158

    摘要： This invention generally relates to methods to obtain mammalian cells and tissues with patterns of gene expression similar to that of a developing mammalian embryo or fetus, and the use of such cells and tissues in the treatment of human disease and age-related conditions. More particularly, the invention relates to methods for identifying, expanding in culture, and formulating mammalian pluripotent stem cells and differentiated cells that differ from cells in the adult human in their pattern of gene expression, and therefore offer unique characteristics that provide novel therapeutic strategies in the treatment of degenerative disease.

    摘要翻译： 本发明一般涉及获得具有与发育中的哺乳动物胚胎或胎儿相似的基因表达模式的哺乳动物细胞和组织的方法，以及这些细胞和组织在治疗人类疾病和年龄相关病症中的用途。 更具体地说，本发明涉及用于鉴定，扩增培养和配制哺乳动物多能干细胞和分化细胞的方法，所述哺乳动物多能干细胞和分化细胞在成年人的细胞基因表达模式中不同，因此提供独特的特征，其提供新颖的治疗策略 退行性疾病的治疗。

    Methods of modifying transcriptional regulatory networks in stem cells
    8.
    发明授权
    Methods of modifying transcriptional regulatory networks in stem cells 有权

    公开(公告)号：US09732128B2

    公开(公告)日：2017-08-15

    申请号：US13279123

    申请日：2011-10-21

    申请人： Michael D. West ,  Karen B. Chapman

    发明人： Michael D. West ,  Karen B. Chapman

    IPC分类号： C12N5/00 ,  C12N5/02 ,  A01N63/00 ,  C07K14/47 ,  C12N5/074

    CPC分类号： C07K14/4702 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603

    摘要： The vast differentiation potential of human embryonic and induced pluripotent stem cells, including their potential to cascade through all of the somatic cell lineages and to display the complete transcriptional regulatory network of human biology, has generated interest in deriving scalable, purified, and identified cell types and methods of discovering the precise structure of the human regulatory network. However, the innate capacity of pluripotent cells to display all these lineages is not necessarily reflected during their culture in vitro. The clonal isolation and propagation of progenitors greatly facilitates the generation of highly purified and identified formulations for research and therapeutic purposes. Nevertheless, other cell types have yet to be isolated and propagated from normal cells and methods of isolating said novel cell types as well as methods for introducing perturbations into the transcriptional regulatory network in order to construct a computer model of the entire human transcriptional regulatory network would greatly benefit basic research as well as manufacturing technology for cell-based therapies.

    Methods of Modifying Transcriptional Regulatory Networks in Stem Cells
    9.
    发明申请
    Methods of Modifying Transcriptional Regulatory Networks in Stem Cells 有权
    修改干细胞转录调控网络的方法

    公开(公告)号：US20120129262A1

    公开(公告)日：2012-05-24

    申请号：US13279123

    申请日：2011-10-21

    申请人： Michael D. West ,  Karen B. Chapman

    发明人： Michael D. West ,  Karen B. Chapman

    IPC分类号： C12N5/071 ,  C12N15/85 ,  C12N15/867 ,  C12N5/02

    CPC分类号： C07K14/4702 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603

    摘要： The vast differentiation potential of human embryonic and induced pluripotent stem cells, including their potential to cascade through all of the somatic cell lineages and to display the complete transcriptional regulatory network of human biology, has generated interest in deriving scalable, purified, and identified cell types and methods of discovering the precise structure of the human regulatory network. However, the innate capacity of pluripotent cells to display all these lineages is not necessarily reflected during their culture in vitro. The clonal isolation and propagation of progenitors greatly facilitates the generation of highly purified and identified formulations for research and therapeutic purposes. Nevertheless, other cell types have yet to be isolated and propagated from normal cells and methods of isolating said novel cell types as well as methods for introducing perturbations into the transcriptional regulatory network in order to construct a computer model of the entire human transcriptional regulatory network would greatly benefit basic research as well as manufacturing technology for cell-based therapies.

    摘要翻译： 人类胚胎和诱导的多能干细胞的巨大分化潜力，包括其通过所有体细胞谱系层叠的潜力和显示人类生物学的完整转录调控网络的巨大分化潜力，已经引起人们对推出可扩展，纯化和鉴定的细胞类型的兴趣 以及发现人类监管网络精确结构的方法。 然而，多能细胞显示所有这些谱系的先天能力在体外培养过程中不一定被反映出来。 祖细胞的克隆分离和繁殖极大地促进了用于研究和治疗目的的高度纯化和鉴定的制剂的产生。 然而，其他细胞类型尚未被分离并从正常细胞传播，并且分离所述新型细胞类型的方法以及将扰动引入转录调控网络的方法以构建整个人转录调控网络的计算机模型 大大有利于基础研究以及基于细胞的疗法的制造技术。

    METHODS FOR IDENTIFYING LIGANDS FOR STEM CELLS AND CELLS DERIVED THEREFROM
    10.
    发明申请
    METHODS FOR IDENTIFYING LIGANDS FOR STEM CELLS AND CELLS DERIVED THEREFROM 审中-公开
    用于识别干细胞和其衍生细胞的配体的方法

    公开(公告)号：US20090215640A1

    公开(公告)日：2009-08-27

    申请号：US11915696

    申请日：2006-05-26

    申请人： Michael D. West ,  Karen B. Chapman ,  David Larocca

    发明人： Michael D. West ,  Karen B. Chapman ,  David Larocca

    IPC分类号： C40B30/04 ,  C12N5/06

    CPC分类号： G01N33/56966

    摘要： The present invention provides methods for the identification of novel ligands to pluripotent stem cells such as human embryonic stem cells, human embryo-derived cells, and from cells differentiated from such cells, and the use of such ligands in identifying differentiation conditions, purifying cells, and for eliminating such cells from mixtures of varied cell types. The invention also provides methods for the identification of target progenitor cells and cells identified thereby.

    摘要翻译： 本发明提供用于鉴定多能干细胞（例如人胚胎干细胞，人胚胎源细胞）和从这些细胞分化的细胞的新配体的方法，以及这些配体在鉴定分化条件，纯化细胞， 并且用于从不同细胞类型的混合物中消除这种细胞。 本发明还提供用于鉴定由此鉴定的靶祖细胞和细胞的方法。