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    Culture of undifferentiated chicken cells
    4.
    发明授权
    Culture of undifferentiated chicken cells 失效
    未分化鸡细胞培养

    公开(公告)号：US07422897B2

    公开(公告)日：2008-09-09

    申请号：US09757054

    申请日：2001-01-08

    申请人： James N. Petitte ,  Yi Guo Zhang

    发明人： James N. Petitte ,  Yi Guo Zhang

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N15/00

    CPC分类号： C12N5/0606 ,  C12N2502/13 ,  C12N2506/04

    摘要： A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype. The method includes the steps of collecting avian gonadal cells including primordial germ cells from an avian embryo after the formation of the primitive streak; depositing the avian gonadal cells in contact with a preconditioned feeder matrix; and growing the avian gonadal cells on the pre-conditioned feeder matrix in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.

    摘要翻译： 一种产生表达胚胎干细胞表型的未分化禽类细胞的方法。 该方法包括在原始条纹形成之后从禽类胚胎收集包括原始生殖细胞的禽类性腺细胞的步骤; 使禽类细胞与预处理的饲养基质接触; 并在培养基存在下在预处理饲养基质上生长足够长的时间，以产生基本上由表达胚胎干细胞表型的未分化禽类细胞组成的禽类细胞培养物。

    Pluripotent mammalian cells
    7.
    发明申请
    Pluripotent mammalian cells 审中-公开
    多能哺乳动物细胞

    公开(公告)号：US20020090722A1

    公开(公告)日：2002-07-11

    申请号：US09881204

    申请日：2001-06-15

    发明人： Tanja Dominko ,  Raymond L. Page ,  Alan Colman ,  Todd Vaught ,  Vivienne Marshall

    IPC分类号： C12N005/08

    CPC分类号： C12N15/873 ,  A61K48/00 ,  C12N5/16 ,  C12N2506/04 ,  C12N2517/04 ,  C12N2517/10

    摘要： The invention relates to a method of making pluripotent stem cells that does not involve the formation of early preimplantation embryos or fetal tissue. The method has general utility in the production of pluripotent stem cells from many mammalian species but has particular application in man where pluripotent stem cell production can be customized to particular human individual. The method involves the fusion of donor somatic or stem cells (or their karyoplasts) with cytoplasmic, membrane-delimited fragments of mammalian oocytes or zygotes. After the initial genomic reprogramming occurs, the cells can proliferate and thus multiply in vitro yielding a large number of autologous cells for cell therapy application. The result of this process is a cell population genomically identical to the somatic, differentiated cells derived from an individual patient. However, these cells are pluripotent in that upon application of specific growth factors, the cells are capable of differentiating into specific cell types as required by the sought clinical indication.

    摘要翻译： 本发明涉及一种制造不涉及早期植入前胚胎或胎儿组织的形成的多能干细胞的方法。 该方法在许多哺乳动物物种的多能干细胞的生产中具有普遍的用途，但在人类中具有特殊应用，其中多能干细胞的生产可以根据具体的个体进行定制。 该方法涉及供体体细胞或干细胞（或它们的核型）与哺乳动物卵母细胞或合子的细胞质膜分离的片段的融合。 在初始基因组重编程发生后，细胞可以增殖并因此在体外繁殖产生大量用于细胞治疗应用的自体细胞。 该过程的结果是与来自个体患者的体细胞分化细胞基因相同的细胞群体。 然而，这些细胞是多能的，因为在应用特定生长因子时，细胞能够根据寻求的临床适应症的要求将其分化成特定的细胞类型。