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公开(公告)号：US09090902B2
公开(公告)日：2015-07-28
申请号：US13060458
申请日：2009-08-25
申请人： Jörg Bauer , Toralf Senger , Thorsten Zank , Xiao Qiu , Guohai Wu
发明人： Jörg Bauer , Toralf Senger , Thorsten Zank , Xiao Qiu , Guohai Wu
CPC分类号： C12N15/8247 , C12N5/14 , C12N9/00 , C12N9/0004
摘要： The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.
摘要翻译： 本发明涉及来自Drechslera tritici-repentis，Cylindorcarpon herteronema，Diploida natalensis，Stagonospora nodorum，Microdochium nivalae和Periplaneta americana的核酸。 本发明还涉及个体编码序列和由这些序列与其它序列组合编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法，以及花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸的生产 酸在植物中。
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公开(公告)号：US10190131B2
公开(公告)日：2019-01-29
申请号：US12280090
申请日：2007-02-21
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
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公开(公告)号：US20110162105A1
公开(公告)日：2011-06-30
申请号：US13060458
申请日：2009-08-25
申请人： Jörg Bauer , Toralf Senger , Thorsten Zank , Xiao Qiu , Guohai Wu
发明人： Jörg Bauer , Toralf Senger , Thorsten Zank , Xiao Qiu , Guohai Wu
IPC分类号： A01H1/06 , C07H21/04 , C12N15/63 , C12N5/10 , C12N1/19 , C12N1/15 , A01H5/00 , A01H5/10 , C12N9/02 , C12P7/64 , C07C57/03
CPC分类号： C12N15/8247 , C12N5/14 , C12N9/00 , C12N9/0004
摘要： The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.
摘要翻译： 本发明涉及来自Drechslera tritici-repentis，Cylindorcarpon herteronema，Diploida natalensis，Stagonospora nodorum，Microdochium nivalae和Periplaneta americana的核酸。 本发明还涉及个体编码序列和由这些序列与其它序列组合编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法，以及花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸的生产 酸在植物中。
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4.发明授权Isolation and characterization of a novel pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains 有权对所有长于18个碳链的所有ω-6脂肪酸具有特异性的新型ium ium ium ome 3去饱和酶的分离和表征公开(公告)号：US09029111B2
公开(公告)日：2015-05-12
申请号：US12438373
申请日：2007-08-16
申请人： Jörg Bauer , Guohai Wu , Xiao Qiu
发明人： Jörg Bauer , Guohai Wu , Xiao Qiu
CPC分类号： C12P7/6472 , A61K8/64 , A61K8/922 , A61K31/202 , A61K2800/10 , A61Q19/00 , C12N9/0006 , C12N9/0083 , C12N9/0093 , C12N15/52 , C12P7/6427 , C12P7/6436
摘要： The present invention relates to a polynucleotide encoding an omega 3 (ω-3) desaturase from Pythium irregulare with specificity to long chain polyunsaturated omega 6 (ω-6) fatty acids as well as a vector containing said polynucleotide, and a host cell containing the vector or the polynucleotide. Moreover, the present invention pertains to a polypeptide encoded by the said polynucleotide, antibodies against the polypeptide as well as a method for the manufacture of the polypeptide. Further, encompassed by the present invention are transgenic non-human organisms. Finally, the present invention relates to methods for the manufacture of compounds and oil- fatty acid- or lipid-containing compositions.
摘要翻译： 本发明涉及编码具有对长链多不饱和ω-6（ω-6）脂肪酸的特异性的不锈菌的ω-3（ω-3）去饱和酶的多核苷酸以及含有所述多核苷酸的载体和含有 载体或多核苷酸。 此外，本发明涉及由所述多核苷酸编码的多肽，针对多肽的抗体以及制备多肽的方法。 此外，本发明包括转基因非人生物体。 最后，本发明涉及制备化合物和含油脂肪酸或含脂质的组合物的方法。
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5.发明授权公开(公告)号：US09458436B2
公开(公告)日：2016-10-04
申请号：US10590457
申请日：2005-02-23
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla
CPC分类号： C12N9/0071 , A23D9/00 , A61K8/361 , A61K8/922 , A61K31/202 , A61K2800/86 , A61Q19/00 , C12N9/0083 , C12N9/1029 , C12N15/8247 , C12Y114/19
摘要： The present invention relates to a process for the production of polyunsaturated fatty acids in the seed of transgenic plants by introducing, into the organism, nucleic acids which encode polypeptides with a ω3-desaturase, Δ12-desaturase, Δ6-desaturase, Δ6-elongase, Δ5-desaturase, Δ5-elongase and/or Δ4-desaturase activity. The invention furthermore relates to recombinant nucleic acid molecules comprising the nucleic acid sequences which encode the aforementioned polypeptides, either jointly or individually, and transgenic plants which comprise the aforementioned recombinant nucleic acid molecules. Furthermore, the invention relates to the generation of a transgenic plant and to oils, lipids and/or fatty acids with an elevated content of polyunsaturated fatty acids, in particular arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, as the result of the expression of the elongases and desaturases used in the process according to the invention.
摘要翻译： 本发明涉及通过向生物体中引入编码具有ω3-去饱和酶，Δ12-去饱和酶，Δ6-去饱和酶，Δ6-延伸酶等的多肽的核酸而在转基因植物的种子中生产多不饱和脂肪酸的方法。 Δ5-去饱和酶，Δ5延伸酶和/或Δ4-去饱和酶活性。 本发明还涉及包含共同或单独编码前述多肽的核酸序列的重组核酸分子以及包含上述重组核酸分子的转基因植物。 此外，本发明涉及转基因植物的产生以及具有升高的多不饱和脂肪酸含量的油，脂和/或脂肪酸，特别是花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸，作为表达的结果 的根据本发明的方法中使用的延伸酶和去饱和酶。
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6.发明授权Method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants 有权在有用的转基因植物中生产花生四烯酸和/或二十碳五烯酸的方法公开(公告)号：US08134046B2
公开(公告)日：2012-03-13
申请号：US11990244
申请日：2006-08-01
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A61K8/361 , A61Q19/00 , C12N9/0083 , C12N9/1029
摘要： The present invention relates to a process for producing arachidonic acid and/or eicosapentaenoic acid in transgenic useful plants by introducing into the plant nucleic acids coding for polypeptides having Δ6-desaturase, Δ6-elongase or Δ5-desaturase activity. Furthermore, a gene coding for an ω3-desaturase is advantageously expressed in said useful plants. In another advantageous embodiment of the process, further nucleic acid sequences coding for polypeptides of fatty acid or lipid metabolism biosynthesis may be expressed in the plants. Particularly advantageous nucleic acid sequences here are those coding for a Δ8-desaturase, Δ12-desaturase, Δ15-desaturase, Δ4-desaturase, Δ9-elongase and/or Δ5-elongase activity.The invention further relates to the use of the oils, lipids and/or fatty acids produced in the process according to the invention in feedstuffs or foodstuffs, cosmetics or pharmaceuticals.
摘要翻译： 本发明涉及在转基因有用植物中通过向植物核酸编码具有＆Dgr.6-去饱和酶，6'-延伸酶或者5'-去饱和酶活性的多肽的方法来生产花生四烯酸和/或二十碳五烯酸的方法。 此外，编码ω3-去饱和酶的基因有利地在所述有用植物中表达。 在该方法的另一有利实施方案中，可以在植物中表达编码脂肪酸多肽或脂质代谢生物合成的其它核酸序列。 这里特别有利的核酸序列是那些编码“去饱和酶”，“去饱和酶”，“去饱和酶”，“去饱和酶”，“去饱和酶”，“去饱和酶”和“Dgr”延长酶和/或“ 本发明还涉及根据本发明的方法中生产的油，脂和/或脂肪酸在饲料或食品，化妆品或药物中的用途。
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7.发明申请公开(公告)号：US20100263088A1
公开(公告)日：2010-10-14
申请号：US12747670
申请日：2008-12-15
申请人： Jörg Bauer , Tom Wetjen , Xiao Qiu , Guohai Wu
发明人： Jörg Bauer , Tom Wetjen , Xiao Qiu , Guohai Wu
CPC分类号： C12N15/8234
摘要： The present invention is concerned with means and methods for allowing tissue specific and, in particular, seed specific expression of genes. The present invention, accordingly, relates to a polynucleotide comprising an expression control sequence which allows seed specific expression of a nucleic acid of interest being operatively linked thereto. Moreover, the present invention contemplates vectors, host cells, non-human transgenic organisms comprising the aforementioned polynucleotide as well as methods and uses of such a polynucleotide.
摘要翻译： 本发明涉及允许组织特异性，特别是基因的种子特异性表达的方法和方法。 因此，本发明涉及包含表达控制序列的多核苷酸，所述表达控制序列允许与其有效连接的目的核酸的种子特异性表达。 此外，本发明考虑了包含上述多核苷酸的载体，宿主细胞，非人转基因生物以及这种多核苷酸的方法和用途。
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公开(公告)号：US20100227924A1
公开(公告)日：2010-09-09
申请号：US12280090
申请日：2007-02-21
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
CPC分类号： C12N15/8247 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , C12Y114/19
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
摘要翻译： 本发明涉及在转基因植物中生产二十烷酸，二十二烷酸和/或二十二酸的方法。 根据所述方法，给植物提供至少一个编码具有＆Dgr .6去饱和酶活性的多肽的核酸序列，至少一个编码具有＆Dgr .6延长酶活性的多肽的核酸序列，至少一个核酸 编码具有＆Dgr。5去饱和酶活性的多肽的酸序列和编码具有＆Dgr.5延伸酶活性的多肽的至少一个核酸序列，编码具有＆Dgr。5延伸酶活性的多肽的核酸序列被修饰 相对于来自该序列的生物体中的核酸序列，使得其适于至少一种类型的植物中的密码子使用。 为了生产二十二碳六烯酸，还将至少一种编码具有＆Dgr。4去饱和酶活性的多肽的核酸序列引入植物中。
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9.发明申请ISOLATION AND CHARACTERIZATION OF A NOVEL PYTHIUM OMEGA 3 DESATURASE WITH SPECIFICITY TO ALL OMEGA 6 FATTY ACIDS LONGER THAN 18 CARBON CHAINS 有权一个新的磷酸三钙脱硫剂的分离和表征对所有欧米茄6脂肪酸长18个碳链具有特异性公开(公告)号：US20100068761A1
公开(公告)日：2010-03-18
申请号：US12438373
申请日：2007-08-16
申请人： Jörg Bauer , Guohai Wu , Xiao Qiu
发明人： Jörg Bauer , Guohai Wu , Xiao Qiu
IPC分类号： C12P21/00 , C07H21/00 , C12N15/63 , C12N1/00 , C12N9/00 , C07K16/00 , A01K67/00 , A01H5/00 , C12P7/64
CPC分类号： C12P7/6472 , A61K8/64 , A61K8/922 , A61K31/202 , A61K2800/10 , A61Q19/00 , C12N9/0006 , C12N9/0083 , C12N9/0093 , C12N15/52 , C12P7/6427 , C12P7/6436
摘要： The present invention relates to a polynucleotide encoding an omega 3 (ω-3) desaturase from Pythium irregulare with specificity to long chain polyunsaturated omega 6 (ω-6) fatty acids as well as a vector containing said polynucleotide, and a host cell containing the vector or the polynucleotide. Moreover, the present invention pertains to a polypeptide encoded by the said polynucleotide, antibodies against the polypeptide as well as a method for the manufacture of the polypeptide. Further, encompassed by the present invention are transgenic non-human organisms. Finally, the present invention relates to methods for the manufacture of compounds and oil- fatty acid- or lipid-containing compositions.
摘要翻译： 本发明涉及编码具有对长链多不饱和ω-6（ω-6）脂肪酸的特异性的不锈菌的ω-3（ω-3）去饱和酶的多核苷酸以及含有所述多核苷酸的载体和含有 载体或多核苷酸。 此外，本发明涉及由所述多核苷酸编码的多肽，针对多肽的抗体以及制备多肽的方法。 此外，本发明包括转基因非人生物体。 最后，本发明涉及制备化合物和含油脂肪酸或含脂质的组合物的方法。
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10.发明申请Method for Producing Arachidonic Acid and/or Eicosapentaenoic Acid in Useful Transgenic Plants 有权在有用的转基因植物中生产花生四烯酸和/或二十碳五烯酸的方法公开(公告)号：US20090234006A1
公开(公告)日：2009-09-17
申请号：US11990244
申请日：2006-08-01
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A61K8/361 , A61Q19/00 , C12N9/0083 , C12N9/1029
摘要： The invention relates to a method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants, according to which nucleic acids, which encode polypeptides with Δ-6-desaturase activity, Δ-6-elongase activity or Δ-5-desaturase activity, are introduced into the plants. As an added advantage, a gene that encodes a Δ-3-desaturase is expressed in the useful plants. In an additional embodiment of the method, additional nucleic acid sequences, which encode polypeptides involved in the biosynthesis of the fatty acid or lipid metabolism, can be expressed in the plants. Particularly advantageous nucleic acid sequences are those that encode Δ-8-desaturase activity, Δ-12-desaturase activity, Δ-15-desaturase activity, Δ-4-desaturase activity, Δ-9-elongase activity and/or Δ-5-elongase activity. The invention also relates to the use of the oils, lipids and/or fatty acids that have been produced by said method in animal feed or foodstuffs, cosmetics or pharmaceutical products.
摘要翻译： 本发明涉及在有用的转基因植物中生产花生四烯酸和/或二十碳五烯酸的方法，根据该方法编码具有Δ-6-去饱和酶活性的多肽，Δ-6-延伸酶活性或Δ-5-去饱和酶活性 ，被引入到植物中。 作为另外的优点，编码Δ-3-去饱和酶的基因在有用植物中表达。 在该方法的另外的实施方案中，编码参与脂肪酸或脂质代谢的生物合成的多肽的另外的核酸序列可以在植物中表达。 特别优选的核酸序列是编码Δ-8-去饱和酶活性，Δ-12-去饱和酶活性，Δ-15-去饱和酶活性，Δ-4-去饱和酶活性，Δ-9-延伸酶活性和/或Delta-5- 延长酶活性。 本发明还涉及通过所述方法在动物饲料或食品，化妆品或药物产品中产生的油，脂和/或脂肪酸的用途。
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