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    METHOD OF PRODUCING A LIPOLYTIC ENZYME
    8.
    发明申请
    METHOD OF PRODUCING A LIPOLYTIC ENZYME 有权
    生产脂肪酶的方法

    公开(公告)号：US20120196002A1

    公开(公告)日：2012-08-02

    申请号：US13265712

    申请日：2010-04-23

    申请人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    发明人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    IPC分类号： A23L1/48 ,  C12N1/15 ,  C12N9/20

    CPC分类号： C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

    摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.

    摘要翻译： 本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中 核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。

    Method of producing a lipolytic enzyme
    9.
    发明授权
    Method of producing a lipolytic enzyme 有权
    产生脂肪分解酶的方法

    公开(公告)号：US08940519B2

    公开(公告)日：2015-01-27

    申请号：US13265712

    申请日：2010-04-23

    申请人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    发明人： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

    IPC分类号： C12N1/20 ,  C12N9/20 ,  C12N15/00 ,  A23L1/03 ,  A23K1/165 ,  C12N15/80

    CPC分类号： C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

    摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

    摘要翻译： 本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中 核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码脂肪分解酶的异源核苷酸序列的条件下培养细胞。