-
1.发明授权公开(公告)号：US07597932B2
公开(公告)日：2009-10-06
申请号：US11855374
申请日：2007-09-14
申请人： Jainamma Krotz , Daniel Smolko , Howard R. Reese , Thomas J. Onofrey , Daguang Wang , Theodore M. Winger , John R. Havens
发明人： Jainamma Krotz , Daniel Smolko , Howard R. Reese , Thomas J. Onofrey , Daguang Wang , Theodore M. Winger , John R. Havens
CPC分类号： C12Q1/002 , B01D67/003 , B01D69/125 , B01D2323/18 , B01D2323/34 , B01D2323/42 , C09D123/26 , C12Q1/003 , G01N33/5438 , G01N33/545
摘要： The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array and providing a polymerization solution that includes a porogen. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker. The porogen is then removed from the permeation layer, thereby creating void spaces in the permeation layer.
摘要翻译： 本发明提供了用于生物测定的活性电子基质装置上的改进的合成聚合物水凝胶渗透层。 本发明包括在微电极阵列上形成渗透层的方法，包括将接头连接到阵列的表面并提供包含致孔剂的聚合溶液的步骤。 然后将阵列的表面与聚合溶液接触，然后聚合溶液在阵列的表面上聚合，形成通过接头连接在阵列表面上的渗透层。 然后从渗透层除去致孔剂，从而在渗透层中产生空隙。
-
2.发明授权公开(公告)号：US06960298B2
公开(公告)日：2005-11-01
申请号：US10014895
申请日：2001-12-10
申请人： Jainamma Krotz , Daniel Smolko , Howard R. Reese , Thomas J. Onofrey , Daguang Wang , Theodore M. Winger , John R. Havens
发明人： Jainamma Krotz , Daniel Smolko , Howard R. Reese , Thomas J. Onofrey , Daguang Wang , Theodore M. Winger , John R. Havens
IPC分类号： G01N33/53 , A61K20060101 , B01D29/00 , B01D39/14 , B01D63/08 , B01J19/00 , C08J5/12 , C12M1/34 , C12Q1/68 , G01N27/26 , G01N27/30 , G01N27/447 , G01N33/543 , G01N33/547 , G01N33/566
CPC分类号： C12Q1/002 , B01D67/003 , B01D69/125 , B01D2323/18 , B01D2323/34 , B01D2323/42 , C09D123/26 , C12Q1/003 , G01N33/5438 , G01N33/545
摘要： The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The permeation layers have a defined porous character, with mesopores in a size range between about 100 nanometers and about 1000 nanometers, and may also have micropores in the micrometer size range. The mesoporous synthetic hydrogel permeation layers demonstrate improved signal intensity and linearity characteristics as compared to nanoporous synthetic hydrogel permeation layers on active electronic matrix devices. In addition, the present invention also provides synthetic polymer hydrogel permeation layers which contain copolymerized attachment sites for nucleic acid probes or other biomolecules.
摘要翻译： 本发明提供了用于生物测定的活性电子基质装置上的改进的合成聚合物水凝胶渗透层。 渗透层具有限定的多孔性质，其中介孔的尺寸范围在约100纳米至约1000纳米之间，并且也可以具有微米尺寸范围内的微孔。 与有源电子矩阵器件上的纳米多孔合成水凝胶渗透层相比，介孔合成水凝胶渗透层显示出改进的信号强度和线性特性。 此外，本发明还提供含有用于核酸探针或其他生物分子的共聚附着位点的合成聚合物水凝胶渗透层。
-
3.发明授权公开(公告)号：US07270850B2
公开(公告)日：2007-09-18
申请号：US11082325
申请日：2005-03-16
申请人： Jainamma Krotz , Daniel J. Smolko , Howard R. Reese , Thomas J. Onofrey , Daguang Wang , Theodore M. Winger , John R. Havens
发明人： Jainamma Krotz , Daniel J. Smolko , Howard R. Reese , Thomas J. Onofrey , Daguang Wang , Theodore M. Winger , John R. Havens
CPC分类号： C12Q1/002 , B01D67/003 , B01D69/125 , B01D2323/18 , B01D2323/34 , B01D2323/42 , C09D123/26 , C12Q1/003 , G01N33/5438 , G01N33/545
摘要： The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array by treating the surface with a linker by vapor deposition and providing a polymerization solution that includes at least one monomer having a polymerizable moiety, a modified streptavidin, a surfactant or porogen, and a cross-linking agent. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker.
摘要翻译： 本发明提供了用于生物测定的活性电子基质装置上的改进的合成聚合物水凝胶渗透层。 本发明包括在微电极阵列上形成渗透层的方法，包括以下步骤：通过气相沉积用接头处理该表面，并提供包含至少一种具有至少一个单体的聚合溶液，将接头连接到阵列的表面上 可聚合部分，修饰的链霉抗生物素蛋白，表面活性剂或致孔剂和交联剂。 然后将阵列的表面与聚合溶液接触，然后聚合溶液在阵列的表面上聚合，形成通过接头连接在阵列表面上的渗透层。
-
4.发明授权公开(公告)号：US08288155B2
公开(公告)日：2012-10-16
申请号：US11777919
申请日：2007-07-13
申请人： John R. Havens , Thomas J. Onofrey , Charles H. Greef , Gregory J. Kevorkian , Jain Krotz , Kristie L. Lykstad , Daniel E. Raymond , Howard R. Reese , Regina Rooney , John J. Scott
发明人： John R. Havens , Thomas J. Onofrey , Charles H. Greef , Gregory J. Kevorkian , Jain Krotz , Kristie L. Lykstad , Daniel E. Raymond , Howard R. Reese , Regina Rooney , John J. Scott
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00641 , B01J2219/00653 , B01J2219/00659 , B01J2219/00713 , B01J2219/0072 , C07B2200/11 , C07C319/02 , C07H21/00 , C40B40/06 , G01N33/54353 , G01N33/545 , C07C323/60
摘要： Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.
摘要翻译： 描述了将生物分子寻址到选择性寻址电极的方法。 提供了覆盖多个选择性寻址电极的渗透层。 渗透层包括适于结合生物分子的反应性基团，并且在结合到生物分子之前需要通过化学转化的活化。 至少一个选择性寻址的电极被偏压，使得在所述至少一个选择性寻址电极的上覆溶液中发生pH变化。 作为pH变化的结果，将至少一个可选寻址电极上方的渗透层的一部分中的反应基团化学转化成活化的反应性基团。 然后将生物分子与穿过活化的反应性基团的至少一个选择性寻址电极重叠的渗透层结合。
-
5.发明申请公开(公告)号：US20090069198A1
公开(公告)日：2009-03-12
申请号：US11777919
申请日：2007-07-13
申请人： John R. Havens , Thomas J. Onofrey , Charles H. Greef , Gregory J. Kevorkian , Jain Krotz , Kristie L. Lykstad , Daniel E. Raymond , Howard R. Reese , Regina Rooney , John J. Scott
发明人： John R. Havens , Thomas J. Onofrey , Charles H. Greef , Gregory J. Kevorkian , Jain Krotz , Kristie L. Lykstad , Daniel E. Raymond , Howard R. Reese , Regina Rooney , John J. Scott
IPC分类号： C40B50/18
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00641 , B01J2219/00653 , B01J2219/00659 , B01J2219/00713 , B01J2219/0072 , C07B2200/11 , C07C319/02 , C07H21/00 , C40B40/06 , G01N33/54353 , G01N33/545 , C07C323/60
摘要： Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.
摘要翻译： 描述了将生物分子寻址到选择性寻址电极的方法。 提供了覆盖多个选择性寻址电极的渗透层。 渗透层包括适于结合生物分子的反应性基团，并且在结合到生物分子之前需要通过化学转化的活化。 至少一个选择性寻址的电极被偏压，使得在所述至少一个选择性寻址电极的上覆溶液中发生pH变化。 作为pH变化的结果，将至少一个选择性寻址电极上方的渗透层的一部分中的反应基团化学转化成活化的反应性基团。 然后将生物分子与穿过活化的反应性基团的至少一个选择性寻址电极重叠的渗透层结合。
-
-
-
-
搜索结果
5 条记录 (耗时 0.038 秒)
