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1.发明申请Automated system for isolating, amplifying and detecting a target nucleic acid sequence 审中-公开用于分离，扩增和检测靶核酸序列的自动化系统公开(公告)号：US20090155808A1
公开(公告)日：2009-06-18
申请号：US12379053
申请日：2009-02-11
CPC分类号： G01N35/0099 , B01L3/50855 , B01L2300/0829 , G01N35/0098 , G01N35/026 , G01N35/028 , G01N35/1065 , G01N35/1074 , G01N2035/00366 , G01N2035/0405 , G01N2035/1025 , G01N2035/1053 , Y10T436/143333
摘要： A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases.
摘要翻译： 提出了一种用于制备和测试靶向核酸的系统和方法。 该系统集成了移液器，提取器，测定读数器和其他组件，包括选择性兼容的铰接机器人臂（SCARA）。 以前分离的诊断工具的这种协同整合创造了一种需要最少的人为干预的系统和方法。 所得到的系统提供了用于诊断疾病的分离，扩增和检测靶核酸的基本更准确和精确的方法。
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公开(公告)号：US20090149337A1
公开(公告)日：2009-06-11
申请号：US12062602
申请日：2008-04-04
CPC分类号： C12Q1/6806 , C12Q1/689 , C12Q2545/114 , C12Q2531/113 , C12Q2545/101
摘要： The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.
摘要翻译： 本发明涉及确保来自核酸生物样品的提取处理的有效性的方法。 本发明的方法能够区分来自样品的核酸提取的可能缺陷以及随后扩增步骤中的可能缺陷。 本发明还涉及用于从生物样品中提取核酸的包装阵列。
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公开(公告)号：US20080311564A1
公开(公告)日：2008-12-18
申请号：US11573119
申请日：2005-08-05
申请人： Thomas L. Fort
发明人： Thomas L. Fort
CPC分类号： C12Q1/705 , C12Q2600/158
摘要： Amplification primers and methods for specific amplification and detection of a CMV target are disclosed. The primer-target binding sequences are useful for amplification and detection of the CMV target in a variety of amplification and detection reactions.
摘要翻译： 公开了用于特异性扩增和检测CMV靶的扩增引物和方法。 引物 - 靶结合序列可用于扩增和检测多种扩增和检测反应中的CMV靶。
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公开(公告)号：US06241980B1
公开(公告)日：2001-06-05
申请号：US08963934
申请日：1997-11-04
IPC分类号： A61K970
CPC分类号： C12Q1/6816 , C12Q1/6806 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , C12Q2527/125
摘要： The present invention relates to methods in which ion exchange resins are used to reduce the amount of substances which interfere with nucleic acid hybridization in samples. The methods also stabilize the samples. Kits containing the ion exchange resins render the methods convenient to use.
摘要翻译： 本发明涉及使用离子交换树脂来减少干扰样品中的核酸杂交的物质的量的方法。 这些方法也使样品稳定。 含有离子交换树脂的试剂盒使方法使用方便。
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公开(公告)号：US06197514B1
公开(公告)日：2001-03-06
申请号：US09289752
申请日：1999-04-12
申请人： Qimin You , Thomas L. Fort
发明人： Qimin You , Thomas L. Fort
IPC分类号： C12Q168
CPC分类号： C07H21/00 , B01J2219/00608 , B01J2219/00614 , B01J2219/0063 , B01J2219/00653 , B01J2219/00722 , C40B40/06
摘要： Amplification primers and methods for specific amplification and detection of a Yersinia enterocolitica target are disclosed. The primer-target binding sequences are useful for amplification and detection of Y. enterocolitica target in a variety of amplification and detection reactions.
摘要翻译： 公开了用于特异性扩增和检测小肠结肠炎耶尔森氏菌的扩增引物和方法。 引物 - 靶结合序列可用于在多种扩增和检测反应中扩增和检测小肠结肠杆菌靶标。
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公开(公告)号：US09696328B2
公开(公告)日：2017-07-04
申请号：US13551830
申请日：2012-07-18
CPC分类号： G01N35/0099 , B01L3/50855 , B01L2300/0829 , G01N35/0098 , G01N35/026 , G01N35/028 , G01N35/1065 , G01N35/1074 , G01N2035/00366 , G01N2035/0405 , G01N2035/1025 , G01N2035/1053 , Y10T436/143333
摘要： A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases.
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7.发明授权Pretreatment method for extraction of nucleic acid from biological samples and kits therefor 失效从生物样品中提取核酸的预处理方法及其试剂盒公开(公告)号：US07601491B2
公开(公告)日：2009-10-13
申请号：US10359179
申请日：2003-02-06
CPC分类号： C12Q1/6806 , C12Q2523/113 , C12Q2527/101 , C12Q2527/125
摘要： The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
摘要翻译： 本发明涉及用于预处理提取核酸的生物样品的方法本发明采用至少一种蛋白质变性剂与一种或多种以下元素的组合形成用于提取核酸的反应混合物：（1） 至少一种非质子溶剂，（2）逐步加热，和（3）样品稀释。
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公开(公告)号：US06583279B1
公开(公告)日：2003-06-24
申请号：US09770532
申请日：2001-01-26
IPC分类号： C07H2100
CPC分类号： C12Q1/706
摘要： Primers and probes derived from the HBV DNA polymerase gene which facilitate detection and/or quantification of all presently known genotypes of HBV. Disclosed sequences may be used in a variety of primer and probe constructs for detection of HBV nucleic acids.
摘要翻译： 源自HBV DNA聚合酶基因的引物和探针，其便于检测和/或定量所有目前已知的HBV基因型。 公开的序列可以用于各种用于检测HBV核酸的引物和探针构建体。
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公开(公告)号：US06265224B1
公开(公告)日：2001-07-24
申请号：US09328575
申请日：1999-06-09
IPC分类号： G01N118
CPC分类号： C12Q1/6816 , C12Q1/6806 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , C12Q2527/125
摘要： The present invention relates to methods in which ion exchange resins are used to reduce the amount of substances which interfere with nucleic acid hybridization in samples. The methods also stabilize the samples. Kits containing the ion exchange resins render the methods convenient to use.
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公开(公告)号：US6166196A
公开(公告)日：2000-12-26
申请号：US503804
申请日：2000-02-14
申请人： Ray A. McMillian , Thomas L. Fort , Qimin You
发明人： Ray A. McMillian , Thomas L. Fort , Qimin You
CPC分类号： C12Q1/689 , Y02A50/451
摘要： Amplification primers and methods for specific amplification and detection of a Campylobacter jejuni and C. coli target are disclosed. The primer-target binding sequences are useful for amplification and detection of C jejuni and C. coli target in a variety of amplification and detection reactions.
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