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公开(公告)号：US20110045574A1
公开(公告)日：2011-02-24
申请号：US12988707
申请日：2009-04-21
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
IPC分类号： C12N7/02 , H01F1/00 , C12N1/20 , C12N15/63 , B01J20/00 , B01J20/26 , C07K16/00 , C07K1/14 , C07H21/00 , C11B3/00
CPC分类号： B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
摘要翻译： 本发明在色谱领域内。 更准确地说，它涉及一种新颖的色谱介质，即由于疏水介质的孔隙率和/或盖的孔隙度，提供了不同盖子的疏水介质，其中排除了一定尺寸的分子。 本发明还涉及用于纯化不分离介质的大分子的分离介质以及进入分离介质并从其中洗脱的小分子的用途。
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公开(公告)号：US08070958B2
公开(公告)日：2011-12-06
申请号：US12692671
申请日：2010-01-25
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。
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公开(公告)号：US20070125711A1
公开(公告)日：2007-06-07
申请号：US10575945
申请日：2004-11-01
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。
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公开(公告)号：US10562007B2
公开(公告)日：2020-02-18
申请号：US12667873
申请日：2008-07-22
申请人： Andreas Axen , Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Nils Norrman
发明人： Andreas Axen , Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Nils Norrman
IPC分类号： B01D15/36 , B01J20/289 , B01J20/32
摘要： The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.
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公开(公告)号：US20100320149A1
公开(公告)日：2010-12-23
申请号：US12667873
申请日：2008-07-22
申请人： Andreas Axen , Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Nils Norrman
发明人： Andreas Axen , Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Nils Norrman
摘要： The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.
摘要翻译： 本发明涉及一种分离基质，其包括载体; 联接到所述支撑件的外部的延伸器; 和与所述增量剂偶联的配体，其中与所述增量剂偶联的载体的部分构成小于分离基质体积的50％。 本发明还包括制备这种分离基质的方法，以及其中使用分离介质的方法。
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公开(公告)号：US08574437B2
公开(公告)日：2013-11-05
申请号：US13575750
申请日：2011-02-16
IPC分类号： B01D15/08
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
摘要翻译： 本发明涉及生产新型色谱介质的方法及其用于生物分子如蛋白质的纯化的用途。 色谱介质包括具有内多孔芯和外壳的壳珠。 该方法包括在珠的核心中提供缓冲配体，并提供靶向用于珠粒外壳中的生物分子结合的结合配体。 该方法使得可以彼此独立地优化结合性质和缓冲性质，这特别有利于生产色谱聚焦介质。
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公开(公告)号：US20130244229A1
公开(公告)日：2013-09-19
申请号：US13990765
申请日：2011-11-29
IPC分类号： G01N1/28
CPC分类号： G01N1/28 , B01J20/28009 , B01J20/286 , B01J20/3208 , B01J20/3217 , B01J20/327 , B01J20/3274 , B01J20/3285 , B01J20/3293 , Y10T436/2525
摘要： The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment.The composition comprises buffering agent(s) enclosed in a first porous matrix with an impenetrability corresponding to a fractionation range for globular proteins and peptides of
摘要翻译： 本发明涉及一种缓冲组合物，更接近地包含当在例如水性环境中施用时提供pH稳定化的多孔基质封闭的缓冲剂的组合物。 组合物包含封闭在第一多孔基质中的缓冲剂，其不透性对应于球状蛋白和肽的分数范围<10000g / mol，优选<5000g / mol，更优选<700g / mol。
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公开(公告)号：US09278297B2
公开(公告)日：2016-03-08
申请号：US13809179
申请日：2011-06-28
CPC分类号： B01D15/361 , C07K1/18
摘要： The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.
摘要翻译： 本发明涉及一种用于在包含具有内多孔芯和外壳的壳珠的介质上运行离子交换色谱的方法，其中内核具有其电荷随pH变化并且壳被带有带电离子交换的配体 配体，该方法包括以下步骤：a）在第一pH下将样品分子吸附在壳配体上; b）通过添加能够增加其与核配体相同符号/类型的电荷的缓冲物质，在第二pH下引起内核配体的排出，同时使离子从 内部核心配体，从而使离子强度的增加从壳体配体取代样品分子即引起洗脱。
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公开(公告)号：US09259729B2
公开(公告)日：2016-02-16
申请号：US13642937
申请日：2011-04-20
IPC分类号： B01J39/16 , B01J47/00 , B01J20/286 , B01J20/32 , B01J20/28
CPC分类号： B01J39/165 , B01J20/28004 , B01J20/286 , B01J20/3242 , B01J20/3293 , B01J39/17 , B01J47/018
摘要： The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalization as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles/D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads/Total volume beads] is in the range 0.05-0.9.
摘要翻译： 本发明涉及产生具有改进的压力 - 流动性质的色谱介质的方法和方法。 更具体地说，本发明涉及双峰粒径分布和层官能化作为改变色谱介质压力流动性质的手段。 本发明涉及一种用于制备具有改进的压力 - 流动性质的色谱介质的方法，包括将具有较小珠粒/颗粒的包含内核和外部功能化壳/盖的大珠粒/颗粒混合，其中颗粒尺寸 大珠粒：[D50V为大颗粒/ D50V为小颗粒]> 1.2，其中柱中大小珠的体积比为[大珠/总体积珠的总体积]在0.05 -0.9。
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公开(公告)号：US20130115709A1
公开(公告)日：2013-05-09
申请号：US13809179
申请日：2011-06-28
IPC分类号： G01N30/02
CPC分类号： B01D15/361 , C07K1/18
摘要： The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.
摘要翻译： 本发明涉及一种用于在包含具有内多孔芯和外壳的壳珠的介质上运行离子交换色谱的方法，其中内核具有其电荷随pH变化并且壳被带有带电离子交换的配体 配体，该方法包括以下步骤：a）在第一pH下将样品分子吸附在壳配体上; b）通过添加能够增加其与核配体相同符号/类型的电荷的缓冲物质，在第二pH下引起内核配体的排出，同时使离子从 内部核心配体，从而使离子强度的增加从壳体配体取代样品分子即引起洗脱。
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