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公开(公告)号：US20050079510A1
公开(公告)日：2005-04-14
申请号：US10767899
申请日：2004-01-28
申请人： Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
发明人： Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US20060134633A1
公开(公告)日：2006-06-22
申请号：US10768729
申请日：2004-01-28
申请人： Yi-Ju Chen , John Leamon , Kenton Lohman , Michael Ronan , Jonathan Rothberg , Maithreyan Srinivasan , Michael Weiner
发明人： Yi-Ju Chen , John Leamon , Kenton Lohman , Michael Ronan , Jonathan Rothberg , Maithreyan Srinivasan , Michael Weiner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
摘要翻译： 本发明涉及DNA测序方法。 更具体地，本发明涉及通过使用封闭和未封闭的测序引物对DNA的有义链和反义链进行测序的方法。 简而言之，这些方法包括将未封闭引物退火至第一条核酸的步骤; 将第二封闭引物退火至核酸的第二链; 用聚合酶沿着第一链延长核酸; 终止第一个测序引物; 解封第二底漆; 并沿着第二链延长核酸。
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公开(公告)号：US20050130173A1
公开(公告)日：2005-06-16
申请号：US10767779
申请日：2004-09-22
申请人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
发明人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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公开(公告)号：US20060040297A1
公开(公告)日：2006-02-23
申请号：US11195254
申请日：2005-08-01
申请人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
发明人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
IPC分类号： C12Q1/68
CPC分类号： B01L3/502761 , B01L3/5027 , B01L3/5085 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G06F19/22
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
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5.发明申请公开(公告)号：US20060228721A1
公开(公告)日：2006-10-12
申请号：US11104781
申请日：2005-04-12
申请人： John Leamon , William Lee , Jan Simons , Brian Desany , Michael Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
发明人： John Leamon , William Lee , Jan Simons , Brian Desany , Michael Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2565/301 , C12Q2531/107 , C12Q2531/101 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114
摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.
摘要翻译： 所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。 本公开中提供的技术允许在一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。 结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。
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6.发明申请公开(公告)号：US20120264632A1
公开(公告)日：2012-10-18
申请号：US13443629
申请日：2012-04-10
申请人： John Harris Leamon , William Lun Lee , Jan Fredrik Simons , Brian Desany , Michael Todd Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
发明人： John Harris Leamon , William Lun Lee , Jan Fredrik Simons , Brian Desany , Michael Todd Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2565/301 , C12Q2531/107 , C12Q2531/101 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114
摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.
摘要翻译： 所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。 本公开中提供的技术允许在一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。 结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。
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